Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner12h01f
(616 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 223 2e-59
SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz... 109 2e-25
SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo... 106 3e-24
SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch... 93 3e-20
SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha... 93 4e-20
SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2... 89 6e-19
SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch... 81 1e-16
SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac... 81 2e-16
SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac... 77 2e-15
SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac... 74 1e-14
SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac... 66 4e-12
SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch... 59 6e-10
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 56 5e-09
SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizos... 38 0.001
SPAC12G12.14c |pfs2||WD repeat protein Pfs2|Schizosaccharomyces ... 30 0.23
SPBC4B4.03 |rsc1||RSC complex subunit Rsc1 |Schizosaccharomyces ... 28 1.2
SPCC1682.11c |||DUF580 family protein|Schizosaccharomyces pombe|... 28 1.2
SPBC16G5.15c |fkh2||fork head transcription factor Fkh2 |Schizos... 27 2.8
SPAC824.03c |||conserved fungal protein|Schizosaccharomyces pomb... 25 8.7
SPBC609.01 |||ribonuclease II |Schizosaccharomyces pombe|chr 2||... 25 8.7
>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
Hus5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 157
Score = 223 bits (545), Expect = 2e-59
Identities = 93/142 (65%), Positives = 116/142 (81%)
Frame = +1
Query: 190 MSGIASARLAEERKAWRKDHPFGFVARPMKNPDGSLNLMTWECAIPGKKGTPWEGGLYKL 369
MS + RL EERK WR+DHPFGF A+P K+ DG L+LM W+ IPGK T WEGGLYKL
Sbjct: 1 MSSLCKTRLQEERKQWRRDHPFGFYAKPCKSSDGGLDLMNWKVGIPGKPKTSWEGGLYKL 60
Query: 370 RMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLLDEEKDWRPAITIKQILLGIQDLL 549
M F ++YP+ PPKC+F PPLFHPNVYPSGTVCLS+L+EE+ W+PAITIKQILLGIQDLL
Sbjct: 61 TMAFPEEYPTRPPKCRFTPPLFHPNVYPSGTVCLSILNEEEGWKPAITIKQILLGIQDLL 120
Query: 550 NEPNVKDPAQAEAYTIYCQNRL 615
++PN+ PAQ EAYT++ ++++
Sbjct: 121 DDPNIASPAQTEAYTMFKKDKV 142
>SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme
E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 151
Score = 109 bits (263), Expect = 2e-25
Identities = 56/141 (39%), Positives = 78/141 (55%)
Frame = +1
Query: 190 MSGIASARLAEERKAWRKDHPFGFVARPMKNPDGSLNLMTWECAIPGKKGTPWEGGLYKL 369
MS A RL + K ++D P G A P+ + N+M W I G TP+E G +KL
Sbjct: 1 MSTTARRRLMRDFKRMQQDPPAGVSASPVSD-----NVMLWNAVIIGPADTPFEDGTFKL 55
Query: 370 RMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLLDEEKDWRPAITIKQILLGIQDLL 549
+ F + YP+ PP KF +FHPNVY +G +CL +L + W P + IL IQ LL
Sbjct: 56 VLSFDEQYPNKPPLVKFVSTMFHPNVYANGELCLDIL--QNRWSPTYDVAAILTSIQSLL 113
Query: 550 NEPNVKDPAQAEAYTIYCQNR 612
N+PN PA AEA ++ +N+
Sbjct: 114 NDPNNASPANAEAAQLHRENK 134
>SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme
Ubc3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 166
Score = 106 bits (254), Expect = 3e-24
Identities = 57/149 (38%), Positives = 78/149 (52%), Gaps = 11/149 (7%)
Frame = +1
Query: 199 IASARLAEERKAWRKDHPFGFVARPMKNPDGSLNLMTWECAIPGKKGTPWEGGLYKLRMI 378
+A RL +E K ++ P G A P D TW+C I G GTP+EGGLY +
Sbjct: 5 MALRRLMKEYKELTENGPDGITAGPSNEDD----FFTWDCLIQGPDGTPFEGGLYPATLK 60
Query: 379 FKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLLDEEKD-----------WRPAITIKQI 525
F DYP PP KFE FHPNVY GTVC+S+L D W P ++++I
Sbjct: 61 FPSDYPLGPPTLKFECEFFHPNVYKDGTVCISILHAPGDDPNMYESSSERWSPVQSVEKI 120
Query: 526 LLGIQDLLNEPNVKDPAQAEAYTIYCQNR 612
LL + +L EPN + A +A ++ ++R
Sbjct: 121 LLSVMSMLAEPNDESGANIDACKMWREDR 149
>SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme
Ubc13|Schizosaccharomyces pombe|chr 1|||Manual
Length = 148
Score = 93.1 bits (221), Expect = 3e-20
Identities = 47/133 (35%), Positives = 74/133 (55%)
Frame = +1
Query: 211 RLAEERKAWRKDHPFGFVARPMKNPDGSLNLMTWECAIPGKKGTPWEGGLYKLRMIFKDD 390
R+ +E + +D P G VA P ++ NL ++ + G + + +EGG + L + D+
Sbjct: 6 RIIKEIETLTRDPPPGIVAAPTED-----NLRYFKITMEGPQQSAYEGGKFHLELFLPDE 60
Query: 391 YPSSPPKCKFEPPLFHPNVYPSGTVCLSLLDEEKDWRPAITIKQILLGIQDLLNEPNVKD 570
YP PP +F ++HPNV G +CLS L +KDW PA+ I+ +LL IQ L+ PN D
Sbjct: 61 YPMMPPNVRFLTKIYHPNVDKLGRICLSTL--KKDWSPALQIRTVLLSIQALMGAPNPDD 118
Query: 571 PAQAEAYTIYCQN 609
P + I+ +N
Sbjct: 119 PLDNDVAKIWKEN 131
>SPBC119.02 |ubc4||ubiquitin conjugating enzyme
Ubc4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 147
Score = 92.7 bits (220), Expect = 4e-20
Identities = 48/138 (34%), Positives = 74/138 (53%)
Frame = +1
Query: 199 IASARLAEERKAWRKDHPFGFVARPMKNPDGSLNLMTWECAIPGKKGTPWEGGLYKLRMI 378
+A R+ E KD P A P+ + +L W+ I G +P+ GG++ L +
Sbjct: 1 MALKRINRELADLGKDPPSSCSAGPVGD-----DLFHWQATIMGPADSPYAGGVFFLSIH 55
Query: 379 FKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLLDEEKDWRPAITIKQILLGIQDLLNEP 558
F DYP PPK F ++HPN+ +G++CL +L ++ W PA+TI ++LL I LL +P
Sbjct: 56 FPTDYPFKPPKVNFTTRIYHPNINSNGSICLDILRDQ--WSPALTISKVLLSICSLLTDP 113
Query: 559 NVKDPAQAEAYTIYCQNR 612
N DP E +Y +R
Sbjct: 114 NPDDPLVPEIAHVYKTDR 131
>SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 176
Score = 88.6 bits (210), Expect = 6e-19
Identities = 42/109 (38%), Positives = 61/109 (55%)
Frame = +1
Query: 283 PDGSLNLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGT 462
PD NL+ W I G T +EG +K+ M F +YP SPP F P++HPNV SG
Sbjct: 52 PDSDSNLLHWAGTITGPSDTYYEGLKFKISMSFPANYPYSPPTIIFTSPMWHPNVDMSGN 111
Query: 463 VCLSLLDEEKDWRPAITIKQILLGIQDLLNEPNVKDPAQAEAYTIYCQN 609
+CL +L ++ W ++ ILL +Q LL EPN P A+A ++ ++
Sbjct: 112 ICLDILKDK--WSAVYNVQTILLSLQSLLGEPNNASPLNAQAAELWSKD 158
>SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme
Ubc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 217
Score = 81.0 bits (191), Expect = 1e-16
Identities = 46/141 (32%), Positives = 71/141 (50%), Gaps = 1/141 (0%)
Frame = +1
Query: 190 MSGIASARLAEERKAWRKDHPFGFVARPMKNPDGSLNLMTWECAIPGKKGTPWEGGLYKL 369
MS S R+A+E ++D G + + L M G +GTP+EGG + +
Sbjct: 1 MSDNRSRRIAKELADVQQDKQAGIQVWTINDDISHLKGM-----FRGPEGTPYEGGYFVV 55
Query: 370 RMIFKDDYPSSPPKCKFEPPLFHPNV-YPSGTVCLSLLDEEKDWRPAITIKQILLGIQDL 546
+ DYP PPK F+ ++HPNV +G +CL +L ++ W P T+K L+ +Q L
Sbjct: 56 DIEIPIDYPFRPPKMNFDTKIYHPNVSSQTGAICLDILKDQ--WSPVYTMKSALISLQSL 113
Query: 547 LNEPNVKDPAQAEAYTIYCQN 609
L P +P A+ +Y QN
Sbjct: 114 LCTPEPSNPQDAQVAQVYLQN 134
>SPBC1105.09 |ubc15||ubiquitin conjugating enzyme
Ubc15|Schizosaccharomyces pombe|chr 2|||Manual
Length = 167
Score = 80.6 bits (190), Expect = 2e-16
Identities = 46/150 (30%), Positives = 76/150 (50%), Gaps = 11/150 (7%)
Frame = +1
Query: 193 SGIASARLAEERKAWRKDHPFGFVARPMKNPDGSLNLMTWECAIPGKKGTPWEGGLYKLR 372
S + L ++ K +K+ P GF + + ++ WE I G + T +EGG +
Sbjct: 3 SSASEQLLRKQLKEIQKNPPQGFSVGLVDDK----SIFEWEVMIIGPEDTLYEGGFFHAT 58
Query: 373 MIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLL-----------DEEKDWRPAITIK 519
+ F DYP PPK KF ++HPNV+P+G VC+S+L D + W P + +
Sbjct: 59 LSFPQDYPLMPPKMKFTTEIWHPNVHPNGEVCISILHPPGDDKYGYEDAGERWLPVHSPE 118
Query: 520 QILLGIQDLLNEPNVKDPAQAEAYTIYCQN 609
IL+ + +L+ PN + PA +A + +N
Sbjct: 119 TILISVISMLSSPNDESPANIDAAKEFREN 148
>SPCC777.10c |ubc12||ubiquitin conjugating enzyme
Ubc12|Schizosaccharomyces pombe|chr 3|||Manual
Length = 177
Score = 77.0 bits (181), Expect = 2e-15
Identities = 33/81 (40%), Positives = 49/81 (60%)
Frame = +1
Query: 346 WEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLLDEEKDWRPAITIKQI 525
++GG +K R+ D+YP PPK K ++HPN+ G VCL++L +DW P + + I
Sbjct: 65 YKGGKFKFRIQIDDNYPHDPPKVKCLNKIYHPNIDIEGNVCLNIL--RQDWNPVLNLNSI 122
Query: 526 LLGIQDLLNEPNVKDPAQAEA 588
L+G+Q L PN +DP EA
Sbjct: 123 LVGLQFLFLSPNAEDPLNKEA 143
>SPAC1250.03 |ubc14||ubiquitin conjugating enzyme
Ubc14|Schizosaccharomyces pombe|chr 1|||Manual
Length = 155
Score = 74.1 bits (174), Expect = 1e-14
Identities = 36/105 (34%), Positives = 55/105 (52%)
Frame = +1
Query: 298 NLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSL 477
NL W C G + + GG + + F DYP PP +F ++HPN G VCL++
Sbjct: 35 NLFHWACTALGPSDSVYAGGKFHFSLKFPLDYPFQPPTIEFTTRIYHPNFDSEGNVCLAI 94
Query: 478 LDEEKDWRPAITIKQILLGIQDLLNEPNVKDPAQAEAYTIYCQNR 612
L +++ ++P+I ++ +L I LL EPN DP A Y +R
Sbjct: 95 L-KQQVFKPSIKLRSVLEQILQLLREPNPDDPLVASIAEQYRNDR 138
>SPBC1198.09 |ubc16||ubiquitin conjugating enzyme
Ubc16|Schizosaccharomyces pombe|chr 2|||Manual
Length = 160
Score = 66.1 bits (154), Expect = 4e-12
Identities = 32/103 (31%), Positives = 54/103 (52%), Gaps = 1/103 (0%)
Frame = +1
Query: 283 PDGSLNLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNV-YPSG 459
P +++ W+ I G TP+EGG + L + + YP SPP F+ + HPN+ + +G
Sbjct: 28 PVTDVDMFHWKAVIEGPTETPYEGGQWVLDIHVHEGYPISPPSVYFQTKIVHPNISWTNG 87
Query: 460 TVCLSLLDEEKDWRPAITIKQILLGIQDLLNEPNVKDPAQAEA 588
VC+ +L + W PA +++ L I LL+ + P +A
Sbjct: 88 EVCMDIL--KTHWSPAWSLQSACLAIISLLSNYDASSPLNVDA 128
>SPBC211.07c |ubc8||ubiquitin conjugating enzyme
Ubc8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 58.8 bits (136), Expect = 6e-10
Identities = 31/99 (31%), Positives = 49/99 (49%), Gaps = 2/99 (2%)
Frame = +1
Query: 298 NLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYP-SGTVCLS 474
N+ + G TP+ GG++K+ + +YP P F +FHPN+ SG+VCL
Sbjct: 28 NMQEFYVRFHGPSETPYSGGIWKVHVELPSEYPWKSPSIGFVNRIFHPNIDELSGSVCLD 87
Query: 475 LLDEEKDWRPAITIKQIL-LGIQDLLNEPNVKDPAQAEA 588
++++ W P + I + + LL PN DP EA
Sbjct: 88 VINQ--TWSPMFDMINIFEVFLPQLLRYPNASDPLNGEA 124
>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 227
Score = 55.6 bits (128), Expect = 5e-09
Identities = 28/90 (31%), Positives = 41/90 (45%), Gaps = 1/90 (1%)
Frame = +1
Query: 298 NLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSL 477
N++ W I G TP+EGG Y +IF DYP PP + P + +CLS
Sbjct: 33 NILEWHYIITGPPDTPYEGGQYHGTLIFPPDYPFKPPAIRMITP--SGRFQTNTRLCLSF 90
Query: 478 LD-EEKDWRPAITIKQILLGIQDLLNEPNV 564
D K W P+ + IL+G+ + +
Sbjct: 91 SDFHPKSWNPSWMVSTILVGLVSFMTSDEI 120
>SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme
Mms2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 139
Score = 37.9 bits (84), Expect = 0.001
Identities = 31/132 (23%), Positives = 57/132 (43%), Gaps = 2/132 (1%)
Frame = +1
Query: 211 RLAEERKAWRKDHPFGFVARPMKNPDGSLNLMTWECAIPGKKGTPWEGGLYKLRMIFKDD 390
+L EE + K + + N D + L W I G + E +Y L++ +
Sbjct: 9 KLLEELEKGEKGLGESSCSYGLTNAD-DITLSDWNATILGPAHSVHENRIYSLKIHCDAN 67
Query: 391 YPSSPPKCKFEPPLFHPNV-YPSGTVCLSLLDEEKDWRPAITIKQILLGI-QDLLNEPNV 564
YP +PP F + P V +G V +D + W+ +++ +LL + +++ + N
Sbjct: 68 YPDAPPIVTFVSRINLPGVDGETGKVNPHKIDCLRHWKREYSMETVLLDLKKEMASSSNR 127
Query: 565 KDPAQAEAYTIY 600
K P E T +
Sbjct: 128 KLPQPPEGSTFF 139
>SPAC12G12.14c |pfs2||WD repeat protein Pfs2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 509
Score = 30.3 bits (65), Expect = 0.23
Identities = 20/58 (34%), Positives = 28/58 (48%), Gaps = 4/58 (6%)
Frame = -3
Query: 419 NLHFGGLDG*SSLKIIRSLYSPPSHGVPFLPGIAHSQVI-KFNEPS---GFFIGLATK 258
N F G+ L + S SP HG P +PG+ H+ + + EPS F GL +K
Sbjct: 442 NPQFSGVLNLPGLGTMPSFPSPYQHGQPQIPGMLHASLSNSYAEPSTQNSFIPGLTSK 499
>SPBC4B4.03 |rsc1||RSC complex subunit Rsc1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 803
Score = 27.9 bits (59), Expect = 1.2
Identities = 17/50 (34%), Positives = 23/50 (46%)
Frame = -1
Query: 361 TAHLPMESPFYQVSHIPKSLNSMNHLDSS*ASRQNQRDGPYAKLSVLQLN 212
T+ +P PFY S +SLN+ H + + PYAKLS N
Sbjct: 613 TSPIPAR-PFYAQSGSLQSLNTTQHSHQISGGHSGRMNVPYAKLSYTSHN 661
>SPCC1682.11c |||DUF580 family protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 574
Score = 27.9 bits (59), Expect = 1.2
Identities = 12/36 (33%), Positives = 22/36 (61%)
Frame = -3
Query: 248 WSLRQAFRSSAKRALAIPDIFYKNNSIYEPSFNQGL 141
W LR+ +S++ + ++F K NSIY +F++ L
Sbjct: 71 WQLREQEQSNSHISRNENELFSKENSIYNGNFSENL 106
>SPBC16G5.15c |fkh2||fork head transcription factor Fkh2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 642
Score = 26.6 bits (56), Expect = 2.8
Identities = 12/39 (30%), Positives = 20/39 (51%)
Frame = +1
Query: 250 PFGFVARPMKNPDGSLNLMTWECAIPGKKGTPWEGGLYK 366
P V+R + L+ TW C++ G+ G +G L+K
Sbjct: 123 PSKVVSRKHAVVEYDLDDQTWNCSVYGRNGIKVDGKLFK 161
>SPAC824.03c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 247
Score = 25.0 bits (52), Expect = 8.7
Identities = 14/34 (41%), Positives = 19/34 (55%), Gaps = 3/34 (8%)
Frame = -3
Query: 581 ACAGSFTLGSLRRS*IPNK---ICFIVIAGRQSF 489
AC GSFT SL+ I N+ +C I + G S+
Sbjct: 137 ACLGSFTSSSLKTLSISNRPLALCRIYVDGFHSY 170
>SPBC609.01 |||ribonuclease II |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1157
Score = 25.0 bits (52), Expect = 8.7
Identities = 11/31 (35%), Positives = 18/31 (58%)
Frame = -3
Query: 506 AGRQSFSSSSSDKHTVPDGYTLGWNSGGSNL 414
A +QS+ + SSD ++P L +NS G +
Sbjct: 249 ASQQSYINHSSDGASLPQSSFLNFNSSGKRV 279
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,521,153
Number of Sequences: 5004
Number of extensions: 55582
Number of successful extensions: 145
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 130
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 136
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 269634532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -