Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner12g13f
(615 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po... 232 3e-62
SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 226 2e-60
SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces... 32 0.057
SPBC15C4.04c |||amino acid permease, unknown 10|Schizosaccharomy... 31 0.10
SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pomb... 30 0.31
SPCC188.11 |prp45|cwf13, snw1, SPCC584.08|transcriptional regula... 29 0.40
SPBC3E7.11c |||DNAJ protein Caj1/Djp1-type|Schizosaccharomyces p... 28 0.93
SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pomb... 27 2.2
SPBC16H5.05c |cyp7|cwf27|cyclophilin family peptidyl-prolyl cis-... 27 2.8
SPBC839.10 |usp107|snu71|U1 snRNP-associated protein Usp107|Schi... 27 2.8
SPAC323.04 |||mitochondrial ATPase |Schizosaccharomyces pombe|ch... 26 5.0
SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces pomb... 25 6.6
SPAC1420.01c ||SPAC56E4.08c|DUF1752 family protein|Schizosacchar... 25 6.6
SPAC30D11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 25 6.6
SPBC6B1.04 |mde4||monopolin-like complex subunit Mde4|Schizosacc... 25 6.6
SPAC3A11.05c |kms1||meiotic spindle pole body protein Kms1|Schiz... 25 8.7
SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pom... 25 8.7
SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein |S... 25 8.7
>SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 232 bits (567), Expect = 3e-62
Identities = 113/183 (61%), Positives = 139/183 (75%), Gaps = 2/183 (1%)
Frame = +2
Query: 71 SVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSSW 250
+ +E+ V AKLAEQAERY+ M MK V T EL+ EERNLLSVAYKNV+GARR+SW
Sbjct: 3 TTSREDAVYLAKLAEQAERYEGMVENMKSVASTDQELTVEERNLLSVAYKNVIGARRASW 62
Query: 251 RVISSIEQKTE--GSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVF 424
R++SSIEQK E G+ + ++ KEYR K+E+EL IC D+L +L+KHLIP A++ ESKVF
Sbjct: 63 RIVSSIEQKEESKGNTAQVELIKEYRQKIEQELDTICQDILTVLEKHLIPNAASAESKVF 122
Query: 425 YLKMKGDYYRYLAEVATGETRHSVVEDSQKAYQDAFEISKAKMQPTHPIRLGLALNFSVF 604
Y KMKGDYYRYLAE A GE R + S + Y+ A EI+ A++ PTHPIRLGLALNFSVF
Sbjct: 123 YYKMKGDYYRYLAEFAVGEKRQHSADQSLEGYKAASEIATAELAPTHPIRLGLALNFSVF 182
Query: 605 YYE 613
YYE
Sbjct: 183 YYE 185
>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 226 bits (552), Expect = 2e-60
Identities = 112/184 (60%), Positives = 139/184 (75%), Gaps = 2/184 (1%)
Frame = +2
Query: 68 MSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSS 247
MS +E V AKLAEQAERY++M MK+V + +LS EERNLLSVAYKN++GARR+S
Sbjct: 1 MSNSRENSVYLAKLAEQAERYEEMVENMKKVACSNDKLSVEERNLLSVAYKNIIGARRAS 60
Query: 248 WRVISSIEQKTE--GSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKV 421
WR+ISSIEQK E G+ R+ + KEYR K+E EL +IC+DVL +L+KHLIP A+ ESKV
Sbjct: 61 WRIISSIEQKEESRGNTRQAALIKEYRKKIEDELSDICHDVLSVLEKHLIPAATTGESKV 120
Query: 422 FYLKMKGDYYRYLAEVATGETRHSVVEDSQKAYQDAFEISKAKMQPTHPIRLGLALNFSV 601
FY KMKGDYYRYLAE GE + S +AY+ A +I+ A++ PT P+RLGLALNFSV
Sbjct: 121 FYYKMKGDYYRYLAEFTVGEVCKEAADSSLEAYKAASDIAVAELPPTDPMRLGLALNFSV 180
Query: 602 FYYE 613
FYYE
Sbjct: 181 FYYE 184
>SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3131
Score = 32.3 bits (70), Expect = 0.057
Identities = 24/104 (23%), Positives = 43/104 (41%), Gaps = 2/104 (1%)
Frame = +2
Query: 50 PLPSSTMSVDKE--ELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKN 223
P+ S TMS E + R + + Y+ MA + E E ++ + LLS Y N
Sbjct: 2997 PIMSITMSDSSAYGEELMRERFEHLLKAYEKMALMVAEQEEFNAKIEDMALKLLSEKYDN 3056
Query: 224 VVGARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVEKELREIC 355
+R+ + +E+ + EY +E+ L++ C
Sbjct: 3057 EAYQAELFYRLSNCVEKVLHNKISITDLKTEYEEILEQTLKKEC 3100
>SPBC15C4.04c |||amino acid permease, unknown 10|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 542
Score = 31.5 bits (68), Expect = 0.10
Identities = 21/67 (31%), Positives = 36/67 (53%), Gaps = 3/67 (4%)
Frame = +2
Query: 71 SVDKEELVQRAKLAEQAERYDDMAAAMKEVT--ETGVELSNEERN-LLSVAYKNVVGARR 241
SV + ++ K ++ E + ++ + +K V+ ET E+SN+E N LL + YK V
Sbjct: 3 SVSNVSVNEQGKFNDKEEGFSNLKS-LKHVSHSETDFEVSNDEDNQLLELGYKPVFKREF 61
Query: 242 SSWRVIS 262
S+W S
Sbjct: 62 STWATFS 68
>SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1207
Score = 29.9 bits (64), Expect = 0.31
Identities = 37/137 (27%), Positives = 65/137 (47%), Gaps = 4/137 (2%)
Frame = +2
Query: 200 LLSVAYKNVVGARRSSWRVISSIEQKTEGSERKQQMAKEYR-VKVEKELR-EICYDVLGL 373
L+S +N A RS + + I + S + ++ + YR VK K L +IC+ +
Sbjct: 968 LVSYVRRNAETAERS--QALPFITRNL-ASIKSHELHEAYRLVKANKILEAQICFRSIIY 1024
Query: 374 LDKHLIPKASNPESKVFYLKMKGDYYRYLAEVATG-ETRHSVVEDSQKAYQDAFEISKAK 550
L L A++ E + + RY+ ++ E R ED+++A + ++ + A
Sbjct: 1025 LA--LTTVANSEEEADEISALIDECCRYIVALSCELERRRLGEEDTKRALELSYYFASAD 1082
Query: 551 MQPTHP-IRLGLALNFS 598
+QP H I L LA+N S
Sbjct: 1083 LQPMHSIIALRLAINAS 1099
>SPCC188.11 |prp45|cwf13, snw1, SPCC584.08|transcriptional regulator
Prp45|Schizosaccharomyces pombe|chr 3|||Manual
Length = 557
Score = 29.5 bits (63), Expect = 0.40
Identities = 27/93 (29%), Positives = 45/93 (48%), Gaps = 3/93 (3%)
Frame = +2
Query: 77 DKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSSWRV 256
+K+E QR + Q R D M + +G S+ + SV+ + +R S+
Sbjct: 320 EKQEKEQRLFMLAQKAREDRMG---RNAASSGP--SHAKPRSTSVSSEERSRSRAGSFSH 374
Query: 257 ISSIEQKTEGSE---RKQQMAKEYRVKVEKELR 346
S E + E SE R+Q++ +E R + EK+LR
Sbjct: 375 HSESENEDEDSEAFRRRQELRRERRRQAEKDLR 407
>SPBC3E7.11c |||DNAJ protein Caj1/Djp1-type|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 355
Score = 28.3 bits (60), Expect = 0.93
Identities = 29/100 (29%), Positives = 51/100 (51%), Gaps = 4/100 (4%)
Frame = +2
Query: 269 EQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLKM-KGD 445
E E+ Q++A+ Y+V + +LRE YD LG + +P A ++ F+ + GD
Sbjct: 43 ENPEAAREKFQKLAEAYQVLSDPKLRE-KYDKLGKVG--AVPDAGFEDAFEFFKNLFGGD 99
Query: 446 YYR-YLAEVATGETRHSVV--EDSQKAYQDAFEISKAKMQ 556
+R Y+ E+ + ++ E KA +D E SK ++Q
Sbjct: 100 SFRDYVGELNLLKELCKMINEEPELKAIEDT-EESKKQLQ 138
>SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1778
Score = 27.1 bits (57), Expect = 2.2
Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 3/49 (6%)
Frame = +2
Query: 371 LLDKHLIPKASNPESKVFYLKMKGDYYR---YLAEVATGETRHSVVEDS 508
+++K IP++ E+K Y + GD+ +L E A E H V+ D+
Sbjct: 1608 MIEKLCIPESWLNEAKALYARYVGDHLNELYFLQEAALYEDAHKVLLDT 1656
>SPBC16H5.05c |cyp7|cwf27|cyclophilin family peptidyl-prolyl
cis-trans isomerase Cyp7|Schizosaccharomyces pombe|chr
2|||Manual
Length = 463
Score = 26.6 bits (56), Expect = 2.8
Identities = 12/39 (30%), Positives = 20/39 (51%)
Frame = +2
Query: 218 KNVVGARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVE 334
KN+ S+ R +SS + K +E + M +Y K+E
Sbjct: 350 KNLENDEESTLRALSSFQSKIRNAEDEDVMDSQYGSKIE 388
>SPBC839.10 |usp107|snu71|U1 snRNP-associated protein
Usp107|Schizosaccharomyces pombe|chr 2|||Manual
Length = 695
Score = 26.6 bits (56), Expect = 2.8
Identities = 28/110 (25%), Positives = 47/110 (42%), Gaps = 3/110 (2%)
Frame = +2
Query: 77 DKEELVQRAKLAEQA-ERYDDMAAAMK--EVTETGVELSNEERNLLSVAYKNVVGARRSS 247
D ++RA A QA E+ + + +K E+ +L LL V + + R S
Sbjct: 265 DVRSRIERA--ARQAREKNEKLLQNVKTSEIPINAADLEGINPELLPVIEEEIRSFRDQS 322
Query: 248 WRVISSIEQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPK 397
+K + + KEY K +++LR+ D+ LL KH I +
Sbjct: 323 ---AMKKREKQRSKDEYASLYKEYTRKEQEKLRKQNDDLQNLLSKHRISR 369
>SPAC323.04 |||mitochondrial ATPase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 487
Score = 25.8 bits (54), Expect = 5.0
Identities = 12/45 (26%), Positives = 22/45 (48%)
Frame = +2
Query: 206 SVAYKNVVGARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVEKE 340
S +Y + G S W+ I ++ K+ G +R ++ Y +KE
Sbjct: 61 SFSYPFLKGKSDSPWQAIQLLDFKSSGQQRAAYYSERYHSFRDKE 105
>SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 952
Score = 25.4 bits (53), Expect = 6.6
Identities = 30/111 (27%), Positives = 45/111 (40%), Gaps = 7/111 (6%)
Frame = +2
Query: 80 KEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVG-ARRSSWRV 256
KE L +L E + D + A + V NLL + YKNV A +
Sbjct: 608 KEHLYSFLQLVEPSFAKSDSSNATESQISESVRKGISIFNLLFIVYKNVCSQAGINPSTK 667
Query: 257 ISSIEQKTEGSE------RKQQMAKEYRVKVEKELREICYDVLGLLDKHLI 391
+ +++ T E + Q +EY+ K E ELR + LL+ LI
Sbjct: 668 LEDLDEHTLSDELTYITKKFVQKDQEYQTK-EIELRNYKITLQSLLEDKLI 717
>SPAC1420.01c ||SPAC56E4.08c|DUF1752 family
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 580
Score = 25.4 bits (53), Expect = 6.6
Identities = 15/42 (35%), Positives = 24/42 (57%), Gaps = 1/42 (2%)
Frame = +3
Query: 15 FSPSDK-GISELVLFHRPRCPSTRKNWCNVPNWPNKLSDMTT 137
FSP +K + +L LFH + PS+++ V N + SD +T
Sbjct: 162 FSPPEKPSMKDLALFHGNKSPSSKETIPKVSN--SNSSDTST 201
>SPAC30D11.02c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 85
Score = 25.4 bits (53), Expect = 6.6
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = -3
Query: 562 CGLHFRLADFKSILVCFL*ILYNRMSGFSCGHFC 461
C L+F L + K+ L+ I+Y + GFS H C
Sbjct: 46 CLLNFSLRENKNYLI----IVYLPIEGFSANHMC 75
>SPBC6B1.04 |mde4||monopolin-like complex subunit
Mde4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 421
Score = 25.4 bits (53), Expect = 6.6
Identities = 16/43 (37%), Positives = 25/43 (58%), Gaps = 2/43 (4%)
Frame = +2
Query: 290 ERKQQMAKEYRVKVEKELREICYDV--LGLLDKHLIPKASNPE 412
E++Q A +YR+KVE+ +I V + L+ L + SNPE
Sbjct: 85 EQEQNEANDYRLKVERLEHKISDYVQEINSLNSQLQIQKSNPE 127
>SPAC3A11.05c |kms1||meiotic spindle pole body protein
Kms1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 607
Score = 25.0 bits (52), Expect = 8.7
Identities = 25/117 (21%), Positives = 45/117 (38%), Gaps = 5/117 (4%)
Frame = +2
Query: 161 TETGVELSNEERNLLSVAYKNVVGARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVEKE 340
T +G+ + +E + L V Y ++ + I + S + + ++ E
Sbjct: 278 TNSGLYSTKKELSALQVRYATLLRKFTDQTKKIEELSLAASRSSENENTIRRLALE-NHE 336
Query: 341 LREICYDVLGLLD-----KHLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRHSV 496
L+ + +D KHLI ++NP+ F D Y EV T+ SV
Sbjct: 337 LKNSNNQLNNHIDDLTREKHLIALSNNPKGDEFLSPSNLDEMVYSKEVGLSFTQPSV 393
>SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1067
Score = 25.0 bits (52), Expect = 8.7
Identities = 10/27 (37%), Positives = 14/27 (51%)
Frame = +3
Query: 30 KGISELVLFHRPRCPSTRKNWCNVPNW 110
+G+ + L RC +T CNVP W
Sbjct: 694 EGVRKSALSSLWRCATTYYKVCNVPQW 720
>SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 971
Score = 25.0 bits (52), Expect = 8.7
Identities = 13/42 (30%), Positives = 18/42 (42%)
Frame = +2
Query: 383 HLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRHSVVEDS 508
H SNP S K KG++ Y A G ++ +DS
Sbjct: 225 HTSKTYSNPSSFATRKKEKGEHLSYAEAAAVGTQAKNIKKDS 266
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,563,510
Number of Sequences: 5004
Number of extensions: 49665
Number of successful extensions: 183
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 177
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 181
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 269634532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -