Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fner12f11f
         (601 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC777.13 |vps35||retromer complex subunit Vps35|Schizosaccharo...   122   4e-29
SPAC890.06 |||nucleoporin Nup157/170|Schizosaccharomyces pombe|c...    29   0.69 
SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces ...    27   1.6  
SPBC4.03c |||COPII-coated vesicle component Sfb3 |Schizosaccharo...    26   4.8  
SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78 |Schizosacch...    26   4.8  
SPAC13G6.08 |||Cdc20/Fizzy family WD repeat protein|Schizosaccha...    25   6.4  
SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|...    25   6.4  
SPAC2E1P5.04c |cwg2|orb7|geranylgeranyltransferase I beta subuni...    25   8.5  

>SPCC777.13 |vps35||retromer complex subunit
           Vps35|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 785

 Score =  122 bits (294), Expect = 4e-29
 Identities = 61/111 (54%), Positives = 75/111 (67%), Gaps = 1/111 (0%)
 Frame = +2

Query: 269 LRTSLLSPKSYYELYMAITDXXXXXXXXXXXXFQKG-RKVADLYELVQYAGNIVPRLYLL 445
           +R S L+PK YYELYM   +               G   + DLYELVQYAG+IVPRLYL+
Sbjct: 1   MRNSALTPKQYYELYMFNMESLRLLGGTLLETHLNGTHNLMDLYELVQYAGSIVPRLYLM 60

Query: 446 ITVGLVYIKTNTNLRRDLLKDLVEMCRGVQHPLRGLFLRNYLLQCTRNVLP 598
           ITVG  Y++T   L R+++ DL++MCRGVQHPLRGLFLR+YLL  TR  LP
Sbjct: 61  ITVGSAYLETPNALVREIMNDLLDMCRGVQHPLRGLFLRHYLLTQTRKGLP 111


>SPAC890.06 |||nucleoporin Nup157/170|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1315

 Score = 28.7 bits (61), Expect = 0.69
 Identities = 15/59 (25%), Positives = 33/59 (55%)
 Frame = +2

Query: 32  VNAYEFSSNLQKNLIWFFNCIGLVTQAQMTNQVSPVEEQEKLLEEALNIVKVQAFQMKR 208
           V+ YE + N     + F++   +++QAQM N  SP+ +      + ++IV + A++ ++
Sbjct: 269 VSCYELTKNGVNRCV-FYSFSSMISQAQMLNATSPLLDPR--TTQIVSIVPIPAYESQQ 324


>SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 3655

 Score = 27.5 bits (58), Expect = 1.6
 Identities = 20/73 (27%), Positives = 31/73 (42%)
 Frame = -3

Query: 443  TGTI*VQCSQHIGPVHKDLQPSFPFGTPPEDTAPGDAVHLLLPYKAHNSSLVITVKFSIH 264
            T  +   C  H+  V K L     + +   + APG   HL+L    HNS  V+       
Sbjct: 1164 TSEVPTVCKDHVMDVLKQLFRKI-YASKDTEIAPGILGHLVLELSNHNS--VVRSSTQKL 1220

Query: 263  PALLMHVSGRPLI 225
             +LL  +S  P++
Sbjct: 1221 LSLLSELSNTPIV 1233


>SPBC4.03c |||COPII-coated vesicle component Sfb3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 891

 Score = 25.8 bits (54), Expect = 4.8
 Identities = 12/35 (34%), Positives = 16/35 (45%)
 Frame = -3

Query: 599 PVKRSLCTAIGNS*GKVLSKDAELRDTSQPDPSID 495
           P    +C A G   G ++   AELR    P P +D
Sbjct: 173 PATNDVCNASGIPLGMIVQPFAELRPDEAPVPVVD 207


>SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 803

 Score = 25.8 bits (54), Expect = 4.8
 Identities = 15/49 (30%), Positives = 26/49 (53%)
 Frame = +2

Query: 137 VEEQEKLLEEALNIVKVQAFQMKRCLDKSKLMDALKHASTMLGELRTSL 283
           VE +EKL  +  ++ + Q        ++ KL+D++K A T L + R  L
Sbjct: 371 VERREKLESKLTDLKEEQDKLSAAWEEERKLLDSIKKAKTELEQARIEL 419


>SPAC13G6.08 |||Cdc20/Fizzy family WD repeat
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 535

 Score = 25.4 bits (53), Expect = 6.4
 Identities = 10/33 (30%), Positives = 23/33 (69%), Gaps = 1/33 (3%)
 Frame = -2

Query: 297 LFGDNSEVLNSPSIVDACFRASINL-DLSRHLF 202
           ++G N+E  N+P+++D   + + N+  +S++LF
Sbjct: 33  VYGQNAERSNTPTLIDPSNKETANVCPISKNLF 65


>SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large
           subunit|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1752

 Score = 25.4 bits (53), Expect = 6.4
 Identities = 8/20 (40%), Positives = 14/20 (70%)
 Frame = +1

Query: 70  PYLVFQLHRISYSGPNDKPG 129
           PY ++QL  +  +GP++ PG
Sbjct: 388 PYNIYQLQELVRNGPDEHPG 407


>SPAC2E1P5.04c |cwg2|orb7|geranylgeranyltransferase I beta subunit
           Cwg2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 355

 Score = 25.0 bits (52), Expect = 8.5
 Identities = 11/33 (33%), Positives = 21/33 (63%), Gaps = 1/33 (3%)
 Frame = +2

Query: 458 LVYIKTN-TNLRRDLLKDLVEMCRGVQHPLRGL 553
           L+++  N + + RDL+K+ VE+C+  Q   R +
Sbjct: 107 LLFLGDNLSRIDRDLIKNFVELCKTSQGHFRSI 139


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,577,958
Number of Sequences: 5004
Number of extensions: 54027
Number of successful extensions: 136
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 128
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 135
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 262236260
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)