Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner12e15r
(758 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 92 7e-20
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 79 9e-16
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 74 3e-14
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 45 1e-05
SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 35 0.014
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 31 0.24
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 31 0.24
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 29 0.72
SPAC1565.07c |||TATA binding protein interacting protein |Schizo... 27 2.2
SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce... 27 2.9
SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit Pst... 26 5.1
SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain |Schizosaccha... 25 8.9
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 92.3 bits (219), Expect = 7e-20
Identities = 48/123 (39%), Positives = 74/123 (60%)
Frame = -2
Query: 757 GXGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFLPIYQAISKARSGDTA 578
G G+I IGD LRA GQNPT +++ + L P E + E FL + +
Sbjct: 19 GTGRIPKTSIGDLLRACGQNPTLAEITEIESTL-PAE-VDMEQFLQVLNRPNGFDMPGDP 76
Query: 577 NDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVH 398
+F++G + FDKD G I ELR++L++LGEKLS++E+++LL+G G +NY +FV
Sbjct: 77 EEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVKDGMVNYHDFVQ 136
Query: 397 LIM 389
+I+
Sbjct: 137 MIL 139
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 78.6 bits (185), Expect = 9e-16
Identities = 44/123 (35%), Positives = 69/123 (56%), Gaps = 3/123 (2%)
Frame = -2
Query: 751 GKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDER--ISFEVFLPIYQAISKARSGDTA 578
G I ++G +R+LGQ+PT ++++ + D I F FL + K + D
Sbjct: 27 GNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLTMMAR--KMKDTDNE 84
Query: 577 NDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLL-QGQEDSQGNINYENFV 401
+ E + FDKDGNG+I+ EL H+L++LGE+LS +EV ++ + D G INYE F
Sbjct: 85 EEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEFS 144
Query: 400 HLI 392
+I
Sbjct: 145 RVI 147
Score = 33.9 bits (74), Expect = 0.025
Identities = 17/62 (27%), Positives = 36/62 (58%), Gaps = 1/62 (1%)
Frame = -2
Query: 574 DFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLL-QGQEDSQGNINYENFVH 398
+F E FD+D +G I+S EL ++ +LG+ + E++ ++ + D G I++ F+
Sbjct: 13 EFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLT 72
Query: 397 LI 392
++
Sbjct: 73 MM 74
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 73.7 bits (173), Expect = 3e-14
Identities = 41/122 (33%), Positives = 71/122 (58%), Gaps = 1/122 (0%)
Frame = -2
Query: 751 GKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFLPIYQAISKARSGDTAND 572
G I + +G LR+LG N T++++ K + L + I + F+ +K R ++ +
Sbjct: 24 GLIPTSHVGSVLRSLGINVTDAELAKLSNEL--GDAIDEKKFMSFVS--NKLRETESEEE 79
Query: 571 FIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQ-GQEDSQGNINYENFVHL 395
+I+ R FDKD +G+I +A+ + TLGEKLSD+EV+ ++Q + G+ +Y +FV
Sbjct: 80 YIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYYDFVQR 139
Query: 394 IM 389
IM
Sbjct: 140 IM 141
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 44.8 bits (101), Expect = 1e-05
Identities = 34/122 (27%), Positives = 54/122 (44%)
Frame = -2
Query: 757 GXGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFLPIYQAISKARSGDTA 578
G G I + L +L Q+ +E + + P I+ FL ++ S
Sbjct: 61 GDGNIGREDVKTMLTSLNQDASEDSINHMFESINPP--INLAAFLTAMGSMLCRIS--PR 116
Query: 577 NDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVH 398
ND +E FD +G I + +R LS++G+++ EVE +L+ S G YE FV
Sbjct: 117 NDLLEAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVESILRSY-TSHGVFYYEKFVD 175
Query: 397 LI 392
I
Sbjct: 176 AI 177
Score = 29.9 bits (64), Expect = 0.41
Identities = 11/40 (27%), Positives = 23/40 (57%)
Frame = -2
Query: 565 EGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQ 446
E DKDG+G I +++ +L++L + S+D + + +
Sbjct: 52 EAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFE 91
>SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 621
Score = 34.7 bits (76), Expect = 0.014
Identities = 15/57 (26%), Positives = 28/57 (49%)
Frame = -2
Query: 556 RHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVHLIMQ 386
RHF+K + ++ E L++LG +E L +S+ + YE F ++M+
Sbjct: 494 RHFEKKKSNMLNEVEFYAALASLGLVYDTEEGTALFHRAANSEEGVTYERFTEIVME 550
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 30.7 bits (66), Expect = 0.24
Identities = 18/64 (28%), Positives = 31/64 (48%), Gaps = 1/64 (1%)
Frame = -2
Query: 574 DFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQG-QEDSQGNINYENFVH 398
D E + FD D + I ELR + LG EV ++L+ + +G + E+FV
Sbjct: 38 DINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVR 97
Query: 397 LIMQ 386
++ +
Sbjct: 98 VMTE 101
Score = 25.4 bits (53), Expect = 8.9
Identities = 14/59 (23%), Positives = 32/59 (54%), Gaps = 2/59 (3%)
Frame = -2
Query: 577 NDFIEGLRHFDKDGNGFISSAE-LRHLLSTLGEKLSDDEVEQLLQ-GQEDSQGNINYEN 407
++ ++ LR FDK G G++ + +R + + E+ +E+++ + +D G I+ N
Sbjct: 73 SEVLKILRDFDKTGKGYLQMEDFVRVMTEKIVERDPLEEIKRAFELFDDDETGKISLRN 131
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 30.7 bits (66), Expect = 0.24
Identities = 16/52 (30%), Positives = 27/52 (51%)
Frame = -2
Query: 550 FDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVHL 395
FD G+I +LR + LGE L+ +++ QL+ + G ++ E F L
Sbjct: 21 FDVTHKGYIDFEDLRRSCAQLGENLTKEQL-QLMLDLAGTNGKVSREEFAEL 71
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 29.1 bits (62), Expect = 0.72
Identities = 19/57 (33%), Positives = 29/57 (50%)
Frame = +2
Query: 458 LYLIITELLSESREQVSQFRRRDEPIAIFVKMAQTLNKVISSVATACFRYGLVNWQK 628
L+L+ L++ +Q + R + IAIF K + +K I SVA R+ L QK
Sbjct: 1377 LHLLFASLVAHKFDQPQHAQTRTKIIAIFFKDLYSPHKEIYSVAIDALRHVLSQNQK 1433
>SPAC1565.07c |||TATA binding protein interacting protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1220
Score = 27.5 bits (58), Expect = 2.2
Identities = 20/66 (30%), Positives = 37/66 (56%), Gaps = 4/66 (6%)
Frame = -2
Query: 634 EVFLPIYQAISKARSGDTANDFI--EGLRHFDKDGNGFISS-AELRHLLSTLGEK-LSDD 467
++F+ + I+K +G + I + RH DK GN F ++ EL +L +G+K L++
Sbjct: 643 DIFMSVTD-ITKIENGTKIYEEILQDCCRHIDKSGNEFTTAYLELLEVLLKVGQKYLAES 701
Query: 466 EVEQLL 449
+E +L
Sbjct: 702 LLEHIL 707
>SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2100
Score = 27.1 bits (57), Expect = 2.9
Identities = 16/48 (33%), Positives = 27/48 (56%)
Frame = -2
Query: 526 ISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVHLIMQG 383
I A++ LLSTL E D ++EQ+ Q + + Y +F+ ++QG
Sbjct: 42 IVKAQILFLLSTLREDQYDTKLEQIRQLINKNAPRV-YHHFLRRLIQG 88
>SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit
Pst2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1075
Score = 26.2 bits (55), Expect = 5.1
Identities = 18/49 (36%), Positives = 26/49 (53%)
Frame = +2
Query: 386 LHDEVNKVLIVDISLRVFLSLQELLYLIITELLSESREQVSQFRRRDEP 532
+HD+V+K S+ VF L LLY + E L +QVS ++R P
Sbjct: 735 MHDDVDKEQFGYSSMYVFFRLFNLLYERLYE-LQRLEDQVSIIQQRIIP 782
>SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 4196
Score = 25.4 bits (53), Expect = 8.9
Identities = 16/67 (23%), Positives = 31/67 (46%)
Frame = +2
Query: 290 LTVSHYGSNCMVTDLVSLQIKVLQQMMT*NSALHDEVNKVLIVDISLRVFLSLQELLYLI 469
L S+Y ++ D V Q+K L Q +S + + + + RV S+ + L+L+
Sbjct: 539 LETSYYNCMTVLEDEVIAQLKSLLQYSKTSSQMFTTLMRFQPLFFRTRVRTSISDCLHLL 598
Query: 470 ITELLSE 490
+ + E
Sbjct: 599 VNRIKQE 605
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,957,428
Number of Sequences: 5004
Number of extensions: 59798
Number of successful extensions: 164
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 153
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 162
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 363302114
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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