Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner12c11f
(618 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_UPI000051A52B Cluster: PREDICTED: similar to Mediator c... 192 7e-48
UniRef50_O43513 Cluster: Mediator of RNA polymerase II transcrip... 149 5e-35
UniRef50_Q95Q17 Cluster: Mediator of RNA polymerase II transcrip... 77 3e-13
UniRef50_Q9LZD7 Cluster: Transcriptional co-activator-like prote... 62 1e-08
UniRef50_Q55DQ5 Cluster: Putative uncharacterized protein; n=1; ... 51 2e-05
UniRef50_Q08278 Cluster: Mediator of RNA polymerase II transcrip... 40 3e-04
UniRef50_Q2GRJ3 Cluster: Mediator of RNA polymerase II transcrip... 44 0.003
UniRef50_UPI000023F560 Cluster: hypothetical protein FG05360.1; ... 38 0.25
UniRef50_Q0SJE8 Cluster: Transposase; n=2; Rhodococcus sp. RHA1|... 34 3.1
UniRef50_Q5BRH8 Cluster: SJCHGC08229 protein; n=1; Schistosoma j... 33 4.1
UniRef50_A2QWI7 Cluster: Mediator of RNA polymerase II transcrip... 33 5.5
UniRef50_Q0U6Z7 Cluster: Mediator of RNA polymerase II transcrip... 33 7.2
>UniRef50_UPI000051A52B Cluster: PREDICTED: similar to Mediator
complex subunit 7 CG31390-PA isoform 1; n=1; Apis
mellifera|Rep: PREDICTED: similar to Mediator complex
subunit 7 CG31390-PA isoform 1 - Apis mellifera
Length = 227
Score = 192 bits (467), Expect = 7e-48
Identities = 91/137 (66%), Positives = 97/137 (70%)
Frame = +2
Query: 206 ETAQVSSLPLPPMQYINFYTDENVRRNRAPLPPRPIHDSYSMFGNSFNADDAIIRSLESQ 385
E QVSSLPLPP+QYIN YTDENVRR RAP PP PIHD+YSMFGN FNADD IIR LE+Q
Sbjct: 5 EAIQVSSLPLPPVQYINLYTDENVRRGRAPRPPLPIHDTYSMFGNVFNADDTIIRPLEAQ 64
Query: 386 GFRRLYPMHFERRRELKKXXXXXXXXXXXXXXXXVHCPDSPKRAEKVEDXXXXXXXXXXX 565
G +RLYP HF+RRRELKK V CPDSP+RAEKVED
Sbjct: 65 GIKRLYPQHFDRRRELKKLNHSLLVNFLDLIDLLVQCPDSPRRAEKVEDLSLLFIHIHHL 124
Query: 566 XNEFRPHQARETLRVMM 616
NEFRPHQARETLRVMM
Sbjct: 125 LNEFRPHQARETLRVMM 141
>UniRef50_O43513 Cluster: Mediator of RNA polymerase II
transcription subunit 7; n=34; Eumetazoa|Rep: Mediator
of RNA polymerase II transcription subunit 7 - Homo
sapiens (Human)
Length = 233
Score = 149 bits (361), Expect = 5e-35
Identities = 73/139 (52%), Positives = 84/139 (60%)
Frame = +2
Query: 200 MSETAQVSSLPLPPMQYINFYTDENVRRNRAPLPPRPIHDSYSMFGNSFNADDAIIRSLE 379
M E QVS+LP PPMQYI YTDEN++ AP PP PI DSY MFGN F DD IIR LE
Sbjct: 1 MGEPQQVSALPPPPMQYIKEYTDENIQEGLAPKPPPPIKDSYMMFGNQFQCDDLIIRPLE 60
Query: 380 SQGFRRLYPMHFERRRELKKXXXXXXXXXXXXXXXXVHCPDSPKRAEKVEDXXXXXXXXX 559
SQG RL+PM F+ ++EL+K + P S KR EK+ED
Sbjct: 61 SQGIERLHPMQFDHKKELRKLNMSILINFLDLLDILIRSPGSIKREEKLEDLKLLFVHVH 120
Query: 560 XXXNEFRPHQARETLRVMM 616
NE+RPHQARETLRVMM
Sbjct: 121 HLINEYRPHQARETLRVMM 139
>UniRef50_Q95Q17 Cluster: Mediator of RNA polymerase II
transcription subunit 7; n=3; Caenorhabditis|Rep:
Mediator of RNA polymerase II transcription subunit 7 -
Caenorhabditis elegans
Length = 251
Score = 77.0 bits (181), Expect = 3e-13
Identities = 39/132 (29%), Positives = 62/132 (46%)
Frame = +2
Query: 218 VSSLPLPPMQYINFYTDENVRRNRAPLPPRPIHDSYSMFGNSFNADDAIIRSLESQGFRR 397
VS P PP +Y + YT + + AP PP P+ + + ++G + +D +I L++ G
Sbjct: 10 VSPFPNPP-EYASAYTSDRINNGSAPPPPHPLTE-FKVYGEEYRLEDDVIAPLKNAGVAE 67
Query: 398 LYPMHFERRRELKKXXXXXXXXXXXXXXXXVHCPDSPKRAEKVEDXXXXXXXXXXXXNEF 577
LY + E+KK + PD P R EK+ D NEF
Sbjct: 68 LYKNKNNWKTEMKKLNRSAIVAFFDLVEILIRAPDHPMREEKMVDLHTIFINMHHLINEF 127
Query: 578 RPHQARETLRVM 613
RP QAR+++R++
Sbjct: 128 RPVQARDSVRIL 139
>UniRef50_Q9LZD7 Cluster: Transcriptional co-activator-like protein;
n=13; Magnoliophyta|Rep: Transcriptional
co-activator-like protein - Arabidopsis thaliana
(Mouse-ear cress)
Length = 168
Score = 61.7 bits (143), Expect = 1e-08
Identities = 37/125 (29%), Positives = 56/125 (44%), Gaps = 2/125 (1%)
Frame = +2
Query: 236 PPMQYINFYTDENVRRNRAPLPPRPIHDSYSMFGNSFNADDAIIRSLESQGFRRLYP--M 409
PP Y Y D + + AP PP PI +Y FG ++ +D ++ SLE QG +LYP
Sbjct: 8 PPPPYYRLYKDFSENTDSAPEPPPPIEGTYVCFGGNYTTED-VLPSLEEQGVPQLYPKDS 66
Query: 410 HFERRRELKKXXXXXXXXXXXXXXXXVHCPDSPKRAEKVEDXXXXXXXXXXXXNEFRPHQ 589
+ + ++EL+ V P + A+++ + N RPHQ
Sbjct: 67 NLDYKKELRSLNRELQLHILELADVLVDRPS--QYAKRIGEISSIFKNLHHLLNSLRPHQ 124
Query: 590 ARETL 604
AR TL
Sbjct: 125 ARATL 129
>UniRef50_Q55DQ5 Cluster: Putative uncharacterized protein; n=1;
Dictyostelium discoideum AX4|Rep: Putative
uncharacterized protein - Dictyostelium discoideum AX4
Length = 345
Score = 51.2 bits (117), Expect = 2e-05
Identities = 35/121 (28%), Positives = 55/121 (45%), Gaps = 3/121 (2%)
Frame = +2
Query: 263 TDENVRRNRAPLPPRP--IHDSYSMFGNSFNADDAIIRSLESQGFRRLYPM-HFERRREL 433
T++ + N+ PP P + +Y FG +++ D ++ SL+ G ++LYP E EL
Sbjct: 132 TNKKIELNKPLAPPLPPKVGANYVQFGQTYSTVD-MLPSLDESGAKQLYPKGDIEPISEL 190
Query: 434 KKXXXXXXXXXXXXXXXXVHCPDSPKRAEKVEDXXXXXXXXXXXXNEFRPHQARETLRVM 613
KK + P + + K+ED N +RPHQARETL +
Sbjct: 191 KKLNRSILFNYLQLLETLIENPKNYQ--SKIEDISLLFINFHHLLNSYRPHQARETLLSI 248
Query: 614 M 616
M
Sbjct: 249 M 249
>UniRef50_Q08278 Cluster: Mediator of RNA polymerase II
transcription subunit 7; n=4; Saccharomycetales|Rep:
Mediator of RNA polymerase II transcription subunit 7 -
Saccharomyces cerevisiae (Baker's yeast)
Length = 222
Score = 40.3 bits (90), Expect(2) = 3e-04
Identities = 32/114 (28%), Positives = 47/114 (41%), Gaps = 8/114 (7%)
Frame = +2
Query: 299 PPRPIHDSYSMFGNSFNADDAIIRSLESQGFRRLYPMHFERR--------RELKKXXXXX 454
PP P + Y FG+ + D + LES G +LY E +EL+K
Sbjct: 68 PPMPKNQQYRAFGSIWQVKDQL-PDLESMGLTQLYKKSTENESTNYQYKIQELRKLLKSL 126
Query: 455 XXXXXXXXXXXVHCPDSPKRAEKVEDXXXXXXXXXXXXNEFRPHQARETLRVMM 616
PD +R KVE+ NE+RPHQ+RE+L +++
Sbjct: 127 LLNYLELIGVLSINPDMYER--KVENIRTILVNIHHLLNEYRPHQSRESLIMLL 178
Score = 26.6 bits (56), Expect(2) = 3e-04
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = +2
Query: 215 QVSSLPLPPMQYINFYTDENVRR 283
+VSSL PP Y+ F+T N+ +
Sbjct: 8 EVSSLYPPPPPYVKFFTQSNLEK 30
>UniRef50_Q2GRJ3 Cluster: Mediator of RNA polymerase II
transcription subunit 7; n=1; Chaetomium globosum|Rep:
Mediator of RNA polymerase II transcription subunit 7 -
Chaetomium globosum (Soil fungus)
Length = 263
Score = 44.0 bits (99), Expect = 0.003
Identities = 33/114 (28%), Positives = 48/114 (42%), Gaps = 8/114 (7%)
Frame = +2
Query: 299 PPRPIHDSYSMFGNSFNADDAIIRSLESQGFRRLYPM--------HFERRRELKKXXXXX 454
PP P ++++F + + + +SLE G +RL P H +R +LKK
Sbjct: 71 PPEPTEGTWTLFTETQTLTETL-QSLEEAGIQRLGPTSDIDRDSKHLDRGFQLKKLVKSL 129
Query: 455 XXXXXXXXXXXVHCPDSPKRAEKVEDXXXXXXXXXXXXNEFRPHQARETLRVMM 616
+ PD AEK+ED NE+RPH ARE L +M
Sbjct: 130 LLNYLELVGLMGYNPDHA--AEKIEDLKILLLNFHHTLNEYRPHHAREQLIQIM 181
>UniRef50_UPI000023F560 Cluster: hypothetical protein FG05360.1;
n=1; Gibberella zeae PH-1|Rep: hypothetical protein
FG05360.1 - Gibberella zeae PH-1
Length = 246
Score = 37.5 bits (83), Expect = 0.25
Identities = 32/116 (27%), Positives = 46/116 (39%), Gaps = 10/116 (8%)
Frame = +2
Query: 299 PPRPIHDSYSMFGNSFNADDAIIRSLESQGFRRLYPM---------HFERRRELKKXXXX 451
P P + +FG+ + DD + +LE QG L F+R ELK+
Sbjct: 66 PAEPADGRWRVFGDQYMLDDKL-PTLEDQGITNLPSSSQSESKDAKRFDRAFELKRLAKS 124
Query: 452 XXXXXXXXXXXXVHCPDSPKRAE-KVEDXXXXXXXXXXXXNEFRPHQARETLRVMM 616
+P AE KV+D NE+RPHQARE++ +M
Sbjct: 125 LLLNFLELSGTLSR---NPSHAEAKVQDLRTLFINFHHILNEYRPHQARESVIALM 177
>UniRef50_Q0SJE8 Cluster: Transposase; n=2; Rhodococcus sp.
RHA1|Rep: Transposase - Rhodococcus sp. (strain RHA1)
Length = 137
Score = 33.9 bits (74), Expect = 3.1
Identities = 25/68 (36%), Positives = 34/68 (50%), Gaps = 1/68 (1%)
Frame = -1
Query: 546 NNKLRSSTFSALFGESGQ*TSKSNKSR-KFASSEWLSFFNSLLRSKCIGYNLRNPWLSNE 370
+N L S F L E S ++KS+ + EWLS+FN R +GY P +
Sbjct: 68 DNALAESFFQGLKREWLHGRSWTSKSQARLELFEWLSYFNRRRRHSALGY--LTPVEFEQ 125
Query: 369 RIIASSAL 346
R+IASS L
Sbjct: 126 RLIASSTL 133
>UniRef50_Q5BRH8 Cluster: SJCHGC08229 protein; n=1; Schistosoma
japonicum|Rep: SJCHGC08229 protein - Schistosoma
japonicum (Blood fluke)
Length = 87
Score = 33.5 bits (73), Expect = 4.1
Identities = 15/36 (41%), Positives = 23/36 (63%)
Frame = +2
Query: 311 IHDSYSMFGNSFNADDAIIRSLESQGFRRLYPMHFE 418
+H S+ F N FN DDAII+ L ++ L+P+ F+
Sbjct: 1 LHASHGDFSN-FNIDDAIIKRLHARNIFELFPVQFK 35
>UniRef50_A2QWI7 Cluster: Mediator of RNA polymerase II
transcription subunit 7; n=9; Eurotiomycetidae|Rep:
Mediator of RNA polymerase II transcription subunit 7 -
Aspergillus niger
Length = 263
Score = 33.1 bits (72), Expect = 5.5
Identities = 18/39 (46%), Positives = 23/39 (58%), Gaps = 1/39 (2%)
Frame = +2
Query: 503 SPKRAE-KVEDXXXXXXXXXXXXNEFRPHQARETLRVMM 616
SP++ E KVED N +RPHQARE+L +MM
Sbjct: 152 SPEQFESKVEDLRNLFINAHHLLNLYRPHQARESLIMMM 190
>UniRef50_Q0U6Z7 Cluster: Mediator of RNA polymerase II
transcription subunit 7; n=1; Phaeosphaeria nodorum|Rep:
Mediator of RNA polymerase II transcription subunit 7 -
Phaeosphaeria nodorum (Septoria nodorum)
Length = 276
Score = 32.7 bits (71), Expect = 7.2
Identities = 14/36 (38%), Positives = 19/36 (52%)
Frame = +2
Query: 497 PDSPKRAEKVEDXXXXXXXXXXXXNEFRPHQARETL 604
P + +K++D NE+RPHQARETL
Sbjct: 167 PTDELKDDKLKDILTMVTNMHALINEYRPHQARETL 202
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 508,514,246
Number of Sequences: 1657284
Number of extensions: 8704052
Number of successful extensions: 18914
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 18465
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 18907
length of database: 575,637,011
effective HSP length: 97
effective length of database: 414,880,463
effective search space used: 44807090004
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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