Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner12b11r
(674 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 238 6e-64
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 238 6e-64
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 238 6e-64
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 80 3e-16
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 49 5e-07
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 26 4.3
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 26 5.7
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 25 7.6
SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces pombe... 25 10.0
SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 2... 25 10.0
SPBC18H10.04c |sce3|tif48|translation initiation factor eIF4B|Sc... 25 10.0
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 238 bits (582), Expect = 6e-64
Identities = 107/139 (76%), Positives = 123/139 (88%)
Frame = -3
Query: 663 VPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLD 484
+PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLD
Sbjct: 301 LPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLD 360
Query: 483 CHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLG 304
CHTAHIACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLG
Sbjct: 361 CHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLG 420
Query: 303 RFAVRDMRQTVAVGVIKAV 247
RFAVRDMRQTVAVGVIKAV
Sbjct: 421 RFAVRDMRQTVAVGVIKAV 439
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 238 bits (582), Expect = 6e-64
Identities = 107/139 (76%), Positives = 123/139 (88%)
Frame = -3
Query: 663 VPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLD 484
+PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLD
Sbjct: 301 LPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLD 360
Query: 483 CHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLG 304
CHTAHIACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLG
Sbjct: 361 CHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLG 420
Query: 303 RFAVRDMRQTVAVGVIKAV 247
RFAVRDMRQTVAVGVIKAV
Sbjct: 421 RFAVRDMRQTVAVGVIKAV 439
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 238 bits (582), Expect = 6e-64
Identities = 107/139 (76%), Positives = 123/139 (88%)
Frame = -3
Query: 663 VPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLD 484
+PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLD
Sbjct: 301 LPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLD 360
Query: 483 CHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLG 304
CHTAHIACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLG
Sbjct: 361 CHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLG 420
Query: 303 RFAVRDMRQTVAVGVIKAV 247
RFAVRDMRQTVAVGVIKAV
Sbjct: 421 RFAVRDMRQTVAVGVIKAV 439
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 79.8 bits (188), Expect = 3e-16
Identities = 46/142 (32%), Positives = 76/142 (53%), Gaps = 1/142 (0%)
Frame = -3
Query: 666 AVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVL 487
++ GD V V+ +++ GYV +KN P F AQ+ +L P ++ GY+ V+
Sbjct: 524 SICGDQVRLRVRGDD-SDVQTGYVLTSTKN-PVHATTRFIAQIAILELPSILTTGYSCVM 581
Query: 486 DCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 307
HTA FA++ K+D+ T + ++ P G I L P+C+E F+++ +
Sbjct: 582 HIHTAVEEVSFAKLLHKLDK-TNRKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYM 640
Query: 306 GRFAVRDMRQTVAVG-VIKAVN 244
GRF +RD TVAVG V+K ++
Sbjct: 641 GRFTLRDQGTTVAVGKVVKILD 662
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 49.2 bits (112), Expect = 5e-07
Identities = 41/138 (29%), Positives = 63/138 (45%)
Frame = -3
Query: 666 AVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVL 487
AV GD V + ++ V +LR G + + +N P + F A++ + G I +G T VL
Sbjct: 461 AVAGDTVTLQLADIEVNQLRPGDILSNYEN-PVRRVRSFVAEIQTFDIHGPILSGSTLVL 519
Query: 486 DCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 307
H+ + K+ K + + S K I L PLC+ +E P L
Sbjct: 520 -----HLGRTVTSVSLKIVTVNNKRSR-HIASRKRALVRISFLDGLFPLCLA--EECPAL 571
Query: 306 GRFAVRDMRQTVAVGVIK 253
GRF +R TVA G++K
Sbjct: 572 GRFILRRSGDTVAAGIVK 589
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 26.2 bits (55), Expect = 4.3
Identities = 28/137 (20%), Positives = 55/137 (40%)
Frame = -3
Query: 666 AVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVL 487
AV GDN G ++++ ++L+RG + P F A +L + T +
Sbjct: 311 AVAGDNCGLLLRSIKREQLKRGMIVAQPGTVAPH--QKFKASFYILTK--EEGGRRTGFV 366
Query: 486 DCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 307
D + + + +++ ++ T + K + GD + P+ +E Q
Sbjct: 367 DKYRPQLYSRTSDVTVEL---THPDPNDSDKMVMPGDNVEMICTLIHPIVIEKGQ----- 418
Query: 306 GRFAVRDMRQTVAVGVI 256
RF VR+ TV ++
Sbjct: 419 -RFTVREGGSTVGTALV 434
>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 25.8 bits (54), Expect = 5.7
Identities = 24/85 (28%), Positives = 42/85 (49%), Gaps = 2/85 (2%)
Frame = +2
Query: 203 SAALVTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDSTH--RGLEGTKLTMAA 376
S +VTLPPPAS ++ T T T + S ++ G+ + +++ + ++++
Sbjct: 183 STDIVTLPPPAS-STSSFSTITNTSMIPSSSSFTTTTGSPYYNTSSFLPSSVISSASLSS 241
Query: 377 SPDLMDFGLTSVDLPVRRSTFSLIS 451
S L +TS PV S+ SL S
Sbjct: 242 SSVLPTSIITSTSTPVTVSSSSLSS 266
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 25.4 bits (53), Expect = 7.6
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = +2
Query: 368 MAASPDLMDFGLTSVDLPVRRSTFS 442
++ +PDL D L+SVD P++ +T S
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51
>SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 972
Score = 25.0 bits (52), Expect = 10.0
Identities = 13/32 (40%), Positives = 14/32 (43%)
Frame = -2
Query: 121 GVSKEKRATNSFLFYIFYKACNVTLFYNLYKV 26
G S E S+ IFY FYNL KV
Sbjct: 762 GASSEMECYFSYCSLIFYYQATTLQFYNLPKV 793
>SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 25.0 bits (52), Expect = 10.0
Identities = 10/37 (27%), Positives = 19/37 (51%)
Frame = +2
Query: 188 PLVAFSAALVTLPPPASLKLTALMTPTATVCLMSRTA 298
P + ++ T+PP S+ T + PT ++ + TA
Sbjct: 73 PSTSHNSTTTTVPPTTSMNTTTTVPPTTSLNTTTTTA 109
>SPBC18H10.04c |sce3|tif48|translation initiation factor
eIF4B|Schizosaccharomyces pombe|chr 2|||Manual
Length = 388
Score = 25.0 bits (52), Expect = 10.0
Identities = 13/41 (31%), Positives = 20/41 (48%)
Frame = +2
Query: 215 VTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDST 337
+ L P +S + TP+AT S+ + P GG D+T
Sbjct: 244 LNLKPRSSSNVNTEATPSATTTTSSKPKRDPFGGAKPVDNT 284
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,752,900
Number of Sequences: 5004
Number of extensions: 55345
Number of successful extensions: 154
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 149
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 153
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 309878492
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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