Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner12a05f
(606 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 120 2e-28
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 81 9e-17
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 59 6e-10
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 44 2e-05
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 39 5e-04
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 37 0.002
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 36 0.006
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 29 0.53
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 28 1.2
SPAC3H1.11 |hsr1||transcription factor Hsr1|Schizosaccharomyces ... 27 2.1
SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate |Schizos... 27 2.8
SPACUNK4.07c |cta4|sev4, SPAPYUK71.01|P-type ATPase, calcium tra... 25 6.5
SPBP16F5.05c |||ribosome biogenesis protein Nop8|Schizosaccharom... 25 8.6
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 120 bits (289), Expect = 2e-28
Identities = 63/159 (39%), Positives = 95/159 (59%), Gaps = 1/159 (0%)
Frame = +2
Query: 131 YLCKAAEEDVLDLTDSDFSAVLSQHDTALVMFYAPWCGHCKRLKPEYAVAAGLLKTDVPP 310
+ C +AE V + + +++ +V FYAPWCGHCK L PEY AA L+ D
Sbjct: 17 FFCASAE--VPKVNKEGLNELITADKVLMVKFYAPWCGHCKALAPEYESAADELEKD--G 72
Query: 311 VALAKVDCTEGGKSTCEQFSVSGYPTLKIFRKGELSSEYNGPRESNGIVKYMRAQVGPSS 490
++L +VDCTE G C ++S+ GYPTL +F+ G+ S+Y+GPR+ + +VKYMR Q+ P+
Sbjct: 73 ISLVEVDCTEEG-DLCSEYSIRGYPTLNVFKNGKQISQYSGPRKHDALVKYMRKQLLPTV 131
Query: 491 KELLTVADFEAFTSK-DEVVVVGFFEKESDLKGEFLKTA 604
K ++ E F K D++ VV FF K+ L + + A
Sbjct: 132 KP-ISKDTLENFVEKADDLAVVAFF-KDQKLNDTYTEVA 168
Score = 66.1 bits (154), Expect = 4e-12
Identities = 42/122 (34%), Positives = 60/122 (49%), Gaps = 5/122 (4%)
Frame = +2
Query: 146 AEEDVLDLTDSDFS-AVLSQHDTALVMFYAPWCGHCKRLKPEYAVAAGLLKTDVPPVALA 322
++ED++ L +F V+ + LV FYAPWCGHCK L P Y A D V +A
Sbjct: 353 SQEDLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEEYSDD-SNVVVA 411
Query: 323 KVDCTEGGKSTCEQFSVSGYPTLKIFRKGELSS--EYNGPRESNGIVKYM--RAQVGPSS 490
K+D TE S S+SG+PT+ F+ + + Y G R + ++ A P
Sbjct: 412 KIDATENDISV----SISGFPTIMFFKANDKVNPVRYEGDRTLEDLSAFIDKHASFEPIK 467
Query: 491 KE 496
KE
Sbjct: 468 KE 469
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 81.4 bits (192), Expect = 9e-17
Identities = 48/152 (31%), Positives = 79/152 (51%), Gaps = 6/152 (3%)
Frame = +2
Query: 68 KAPAKFKMFGSLKFVLLLGIIYLCKAAEEDVLDLTDSDFSAVLSQHDTALVMFYAPWCGH 247
+ P F +F + F L+ G+ + ++L +F + +LV+FYAPWCG+
Sbjct: 4 RIPTLFTLFLAC-FSLVSGVFGYSPMFGSNTIELNSKNFRKFVKAKGPSLVVFYAPWCGY 62
Query: 248 CKRLKPEYAVAAGLLKTDVPPVALAKVDC-TEGGKSTCEQFSVSGYPTLKIF---RKGE- 412
CK+L P Y A L + +P A VDC + ++ C Q+ V G+PT+K+ KG
Sbjct: 63 CKKLVPTYQKLASNLHSLLPVTA---VDCDADQNRAVCSQYQVQGFPTIKLVYPSSKGSS 119
Query: 413 -LSSEYNGPRESNGIVKYMRAQVGPSSKELLT 505
S++YNG R + K++ + PS ++LT
Sbjct: 120 LSSTDYNGDRSYKSLQKFVSDSI-PSKVKILT 150
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 58.8 bits (136), Expect = 6e-10
Identities = 33/112 (29%), Positives = 54/112 (48%), Gaps = 3/112 (2%)
Frame = +2
Query: 155 DVLDLTDSDFS-AVLSQHDTALVMFYAPWCGHCKRLKPEYAVAAGLLKTDVPPVALAKVD 331
+V++L +F V+ LV FYA WCG+CKRL P Y + K + P V + K++
Sbjct: 141 NVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTYETLGKVFKNE-PNVEIVKIN 199
Query: 332 CTEGGKSTCEQFSVSGYPTLKIFRKGELSSE--YNGPRESNGIVKYMRAQVG 481
+ V+ +PT+K F K + Y G R +++Y+ + G
Sbjct: 200 -ADVFADIGRLHEVASFPTIKFFPKDDKDKPELYEGDRSLESLIEYINKKSG 250
Score = 58.0 bits (134), Expect = 1e-09
Identities = 29/98 (29%), Positives = 52/98 (53%), Gaps = 1/98 (1%)
Frame = +2
Query: 212 ALVMFYAPWCGHCKRLKPEYAVAAGLLKTDVPPVALAKVDCTEGGKSTCEQFSVSGYPTL 391
AL+ FYA WCGHCK L P Y G L D V + K+D + +++ ++G+PTL
Sbjct: 42 ALIEFYATWCGHCKSLAPVYE-ELGALFEDHNDVLIGKID-ADTHSDVADKYHITGFPTL 99
Query: 392 KIF-RKGELSSEYNGPRESNGIVKYMRAQVGPSSKELL 502
F G +Y+ R+ + + +++ + G ++++
Sbjct: 100 IWFPPDGSEPVQYSNARDVDSLTQFVSEKTGIKKRKIV 137
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 44.0 bits (99), Expect = 2e-05
Identities = 32/128 (25%), Positives = 60/128 (46%), Gaps = 4/128 (3%)
Frame = +2
Query: 167 LTDSDFSAVLSQHDTALVMFYAPWCGHCKRLKPEYAVAAGLLKTDV--PPVALAKVDCTE 340
LTD+D + +S+ T + +Y P CG CKRL P + K V +VDC++
Sbjct: 31 LTDNDLESEVSK-GTWFIKYYLPSCGACKRLGPMWDNMVEKAKEQVEGSNFHFGEVDCSK 89
Query: 341 GGKSTCEQFSVSGYPTLKIFRKGELSSE--YNGPRESNGIVKYMRAQVGPSSKELLTVAD 514
S+C ++ PTL +++ GE+ E + ++ ++ + P + + +D
Sbjct: 90 -ELSSCA--NIRAVPTLYLYQNGEIVEEVPFGASTSEASLLDFVETHLNPDTDPDIP-SD 145
Query: 515 FEAFTSKD 538
+ T +D
Sbjct: 146 EDVLTDED 153
Score = 40.7 bits (91), Expect = 2e-04
Identities = 30/134 (22%), Positives = 58/134 (43%)
Frame = +2
Query: 173 DSDFSAVLSQHDTALVMFYAPWCGHCKRLKPEYAVAAGLLKTDVPPVALAKVDCTEGGKS 352
D+D A L+ + + FY+ C C + + A ++ + +A ++C K
Sbjct: 288 DADIDAALTDKEGWFIQFYSSECDDCDDVSTAWYAMANRMRGKLN---VAHINCAVS-KR 343
Query: 353 TCEQFSVSGYPTLKIFRKGELSSEYNGPRESNGIVKYMRAQVGPSSKELLTVADFEAFTS 532
C+Q+S+ +PT +F K E EY G +V + +E+ + A +
Sbjct: 344 ACKQYSIQYFPTF-LFFKEEAFVEYVGLPNEGDLVSFAEEAANFEIREVELLDTVNAEKN 402
Query: 533 KDEVVVVGFFEKES 574
D V + F++ +S
Sbjct: 403 GD-VFFLYFYDDDS 415
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 39.1 bits (87), Expect = 5e-04
Identities = 22/86 (25%), Positives = 37/86 (43%)
Frame = +2
Query: 176 SDFSAVLSQHDTALVMFYAPWCGHCKRLKPEYAVAAGLLKTDVPPVALAKVDCTEGGKST 355
S+F +++ Q +V F+A WCG CK + P++ KVD + +
Sbjct: 9 SEFKSIVCQDKLVVVDFFATWCGPCKAIAPKFE----QFSNTYSDATFIKVDVDQLSEIA 64
Query: 356 CEQFSVSGYPTLKIFRKGELSSEYNG 433
E V P+ +++ GE E G
Sbjct: 65 AEA-GVHAMPSFFLYKNGEKIEEIVG 89
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 37.1 bits (82), Expect = 0.002
Identities = 23/78 (29%), Positives = 35/78 (44%)
Frame = +2
Query: 179 DFSAVLSQHDTALVMFYAPWCGHCKRLKPEYAVAAGLLKTDVPPVALAKVDCTEGGKSTC 358
D++ +S +V FYA WCG CK LKP L + V+ +
Sbjct: 27 DYNTRISADKVTVVDFYADWCGPCKYLKP----FLEKLSEQNQKASFIAVN-ADKFSDIA 81
Query: 359 EQFSVSGYPTLKIFRKGE 412
++ V PT+ +FRKG+
Sbjct: 82 QKNGVYALPTMVLFRKGQ 99
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 35.5 bits (78), Expect = 0.006
Identities = 20/62 (32%), Positives = 26/62 (41%)
Frame = +2
Query: 227 YAPWCGHCKRLKPEYAVAAGLLKTDVPPVALAKVDCTEGGKSTCEQFSVSGYPTLKIFRK 406
YA WCG CK + P ++ A K P AKV+ E + V PT F
Sbjct: 27 YADWCGPCKAISPLFSQLAS--KYASPKFVFAKVNVDE-QRQIASGLGVKAMPTFVFFEN 83
Query: 407 GE 412
G+
Sbjct: 84 GK 85
>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 29.1 bits (62), Expect = 0.53
Identities = 30/107 (28%), Positives = 50/107 (46%), Gaps = 1/107 (0%)
Frame = -1
Query: 606 SAVFRNSP-FRSDSFSKNPTTTTSSLEVKASKSATVRSSLELGPTWARMYLTMPLDSLGP 430
SA F +SP F S+S S PT+ SS+ S +++ ++L T + +T G
Sbjct: 447 SATFTSSPPFYSNS-SVIPTSAPSSVSSFTSSNSSYTTTL----TASNTTVTFTGTGTGS 501
Query: 429 LYSEESSPFLNIFSVGYPDTENCSQVLLPPSVQSTLASATGGTSVFN 289
+ SSP+ + S+ P T + S + S + S+ GTS +
Sbjct: 502 ATATSSSPYYSNSSIIVPTTVSTSGSVSSFSSSPSPTSSFSGTSALS 548
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 27.9 bits (59), Expect = 1.2
Identities = 17/73 (23%), Positives = 29/73 (39%)
Frame = +2
Query: 215 LVMFYAPWCGHCKRLKPEYAVAAGLLKTDVPPVALAKVDCTEGGKSTCEQFSVSGYPTLK 394
L+ FYAPW CK++ + A D K++ E E F V+ P
Sbjct: 24 LLNFYAPWAAPCKQMNQVFDQFA----KDTKNAVFLKIE-AEKFSDIAESFDVNAVPLFV 78
Query: 395 IFRKGELSSEYNG 433
+ ++ + +G
Sbjct: 79 LIHGAKVLARISG 91
>SPAC3H1.11 |hsr1||transcription factor Hsr1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 582
Score = 27.1 bits (57), Expect = 2.1
Identities = 22/78 (28%), Positives = 33/78 (42%), Gaps = 4/78 (5%)
Frame = -1
Query: 591 NSPFRSDSFSKN---PTTTTSSLEVKASKSATVRSS-LELGPTWARMYLTMPLDSLGPLY 424
N PF+ S++ N P+ TT ++ S TV S L + + S GP Y
Sbjct: 270 NYPFQQPSYNPNALVPSYTTLVSQLPPSPCLTVSSGPLSTASSIPSNCSCPSVKSSGPSY 329
Query: 423 SEESSPFLNIFSVGYPDT 370
E +N ++ G P T
Sbjct: 330 HAEQEVNVNSYNGGIPST 347
>SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 640
Score = 26.6 bits (56), Expect = 2.8
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = +3
Query: 159 FSILQIPTFRLFYLNMIQPWSCF 227
F LQ+ FR + N+++PW CF
Sbjct: 208 FYCLQLQMFRKMH-NIVRPWDCF 229
>SPACUNK4.07c |cta4|sev4, SPAPYUK71.01|P-type ATPase, calcium
transporting Cta4 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1211
Score = 25.4 bits (53), Expect = 6.5
Identities = 17/50 (34%), Positives = 27/50 (54%)
Frame = +2
Query: 149 EEDVLDLTDSDFSAVLSQHDTALVMFYAPWCGHCKRLKPEYAVAAGLLKT 298
EE V+D+ + D +AVL L + +P+ CK P+ V A +L+T
Sbjct: 324 EEAVIDVDELDKNAVLFGGTRVLQVTQSPF---CKLKTPDNGVPAIVLRT 370
>SPBP16F5.05c |||ribosome biogenesis protein
Nop8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 146
Score = 25.0 bits (52), Expect = 8.6
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +2
Query: 125 IIYLCKAAEEDVLDLTDSDFSAVLSQHD 208
+IY C+AA+E++LD LS+ D
Sbjct: 6 LIYACRAADEELLDEIIEKCPQELSRRD 33
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,469,162
Number of Sequences: 5004
Number of extensions: 49550
Number of successful extensions: 170
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 153
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 165
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 266270664
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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