Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner12a01f
(531 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1952.17c ||SPAC890.01c|GTPase activating protein|Schizosacch... 115 4e-27
SPBC530.01 |gyp1||GTPase activating protein Gyp1 |Schizosaccharo... 62 4e-11
SPBC2F12.13 |klp5|sot1|kinesin-like protein Klp5|Schizosaccharom... 27 1.7
SPCC4G3.19 |alp16||gamma tubulin complex subunit Alp16 |Schizosa... 26 3.0
SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig... 26 4.0
SPAC1782.01 ||SPAPYUG7.07|proteasome component|Schizosaccharomyc... 25 7.0
SPAC3G9.01 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||... 25 7.0
SPAC23H4.14 |vam6|vps39|guanyl-nucleotide exchange factor Vma6|S... 25 7.0
SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 25 9.3
SPBC582.10c |||ATP-dependent DNA helicase Rhp16b |Schizosaccharo... 25 9.3
>SPAC1952.17c ||SPAC890.01c|GTPase activating
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 619
Score = 115 bits (277), Expect = 4e-27
Identities = 54/136 (39%), Positives = 85/136 (62%), Gaps = 11/136 (8%)
Frame = +2
Query: 143 HKARIKAFEEVLD-QDVIDMQQLQKLAFNGIPDDKGLRSLVWKILLHYLPQEKNARETTL 319
+K RI+ F+++L+ ++ I + L L GIPD+ LR+ W ++L +LP +++ ++ L
Sbjct: 3 YKQRIEKFKDILNSEEPISLPGLCSLCIQGIPDEYSLRAKAWMLMLEFLPTDRSNWQSVL 62
Query: 320 IKKRQLYKQFIEEIIVSP----------GGPTDHPLNMSPDSSWSTYFKDNEVLLQIDKD 469
K R+ Y F++E+++ P G +DHPLN S DS W YF DN++L QIDKD
Sbjct: 63 EKHRKTYTSFVQELLIDPWRKLTLHEESGENSDHPLNTSDDSKWKEYFDDNQILEQIDKD 122
Query: 470 VRRLCPDISFFQSATE 517
+RR PD+SFFQ +E
Sbjct: 123 IRRTLPDLSFFQGKSE 138
>SPBC530.01 |gyp1||GTPase activating protein Gyp1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 514
Score = 62.5 bits (145), Expect = 4e-11
Identities = 30/64 (46%), Positives = 41/64 (64%)
Frame = +2
Query: 149 ARIKAFEEVLDQDVIDMQQLQKLAFNGIPDDKGLRSLVWKILLHYLPQEKNARETTLIKK 328
+RIK F +LD ++D+ L+ LA+NGIP + R +VWK LL YLP + RE TL +K
Sbjct: 190 SRIKKFSRILDAPIVDLNALRTLAWNGIPSEH--RPIVWKYLLGYLPCNASRREVTLKRK 247
Query: 329 RQLY 340
R Y
Sbjct: 248 RDEY 251
>SPBC2F12.13 |klp5|sot1|kinesin-like protein
Klp5|Schizosaccharomyces pombe|chr 2|||Manual
Length = 883
Score = 27.1 bits (57), Expect = 1.7
Identities = 17/65 (26%), Positives = 31/65 (47%), Gaps = 1/65 (1%)
Frame = +2
Query: 224 NGIPDDKGLRSLVWKILLHYLPQEKNARETTL-IKKRQLYKQFIEEIIVSPGGPTDHPLN 400
+G D GL L K L + ++ + L + ++Y + I +++VSP PLN
Sbjct: 155 SGTMQDPGLIYLTLKELFERMDHLRDEKIFDLRLSYLEIYNETIRDLLVSPTPNQAKPLN 214
Query: 401 MSPDS 415
+ D+
Sbjct: 215 LREDA 219
>SPCC4G3.19 |alp16||gamma tubulin complex subunit Alp16
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 759
Score = 26.2 bits (55), Expect = 3.0
Identities = 13/31 (41%), Positives = 18/31 (58%)
Frame = +2
Query: 395 LNMSPDSSWSTYFKDNEVLLQIDKDVRRLCP 487
+NM+PD F DN + L+ DVR +CP
Sbjct: 466 MNMTPD------FNDNLLQLKFTDDVRNVCP 490
>SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase
E3 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1647
Score = 25.8 bits (54), Expect = 4.0
Identities = 13/37 (35%), Positives = 23/37 (62%)
Frame = +2
Query: 248 LRSLVWKILLHYLPQEKNARETTLIKKRQLYKQFIEE 358
L+SL + L +Y +E+N +TL++ +QL K + E
Sbjct: 837 LKSLAKEFLSNY--KEENLENSTLVQLKQLSKHLLSE 871
>SPAC1782.01 ||SPAPYUG7.07|proteasome component|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1679
Score = 25.0 bits (52), Expect = 7.0
Identities = 15/30 (50%), Positives = 16/30 (53%)
Frame = +3
Query: 438 ITRYCCKSTRTCEDSAPIYHSFNLLLNFLV 527
I RYC T T IYHSF L+FLV
Sbjct: 872 IVRYCEPQTYTMTRLNDIYHSF---LSFLV 898
>SPAC3G9.01 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 462
Score = 25.0 bits (52), Expect = 7.0
Identities = 13/31 (41%), Positives = 17/31 (54%)
Frame = +2
Query: 323 KKRQLYKQFIEEIIVSPGGPTDHPLNMSPDS 415
KKR + + IVSPG H LN +P+S
Sbjct: 384 KKRSKRVLWSLKHIVSPGNREKHSLNSTPES 414
>SPAC23H4.14 |vam6|vps39|guanyl-nucleotide exchange factor
Vma6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 905
Score = 25.0 bits (52), Expect = 7.0
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = +2
Query: 266 KILLHYLPQEKNARETTLIKKRQLYKQFIEEI 361
++ L YL + ETT K ++++KQ IE++
Sbjct: 661 RLALLYLKRILELEETTDFKNQEVFKQTIEKL 692
>SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 676
Score = 24.6 bits (51), Expect = 9.3
Identities = 12/29 (41%), Positives = 16/29 (55%)
Frame = -2
Query: 176 VLLQRLLS*LYGDSYCFYIIKSLVFTHYS 90
V + L S + Y FY I +L+FT YS
Sbjct: 475 VCIASLYSAFWPVIYRFYFISALIFTSYS 503
>SPBC582.10c |||ATP-dependent DNA helicase Rhp16b
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 830
Score = 24.6 bits (51), Expect = 9.3
Identities = 16/48 (33%), Positives = 27/48 (56%), Gaps = 4/48 (8%)
Frame = +2
Query: 230 IPDDKGLRSLVWKILL---HYLPQEKNARETTL-IKKRQLYKQFIEEI 361
+ DD GL + I L H LP++K++ ++TL + L KQ+ E+
Sbjct: 260 LADDMGLGKTIQMIALILSHPLPKKKHSIKSTLVVAPLSLIKQWESEV 307
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,993,566
Number of Sequences: 5004
Number of extensions: 37729
Number of successful extensions: 131
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 127
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 129
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 218398248
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -