Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner11k15r
(739 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 108 7e-25
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 82 7e-17
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 82 7e-17
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 82 7e-17
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 71 1e-13
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 42 9e-05
SPAC11E3.02c |||C2 domain protein|Schizosaccharomyces pombe|chr ... 27 3.7
SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharo... 26 6.4
SPAC20G8.10c ||SPAC3A12.01c|beclin family protein|Schizosaccharo... 26 6.4
SPBC11B10.02c |his3||histidinol-phosphate aminotransferase imida... 26 6.4
SPBC23E6.01c ||SPBPJ758.01|RNA-binding protein, rrm type|Schizos... 26 6.4
SPBC19G7.01c |msh2|swi8, mut3, SPBC24C6.12c|MutS protein homolog... 26 6.4
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 25 8.5
SPCC330.07c |||membrane transporter|Schizosaccharomyces pombe|ch... 25 8.5
SPAC3G9.13c |msw1||mitochondrial tryptophan-tRNA ligase Msw1 |Sc... 25 8.5
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 108 bits (260), Expect = 7e-25
Identities = 63/183 (34%), Positives = 94/183 (51%), Gaps = 1/183 (0%)
Frame = -2
Query: 708 GFCVSGRIENGVLKKGDKVLVCPTKEMAEVRSLSIN-DLTNNVAFAGDQVTLTLSGVDMQ 532
G + G+IE G +KK VLV P + EV ++ D + + GDQV L + G D
Sbjct: 481 GTILEGKIEAGSIKKNSNVLVMPINQTLEVTAIYDEADEEISSSICGDQVRLRVRGDD-S 539
Query: 531 NVSVGYILSDPIEQVSVTTRFEARIVVFNVKVPITKGFPVLVHYQSLVESANIVKLKALL 352
+V GY+L+ V TTRF A+I + + +T G+ ++H + VE + KL L
Sbjct: 540 DVQTGYVLTSTKNPVHATTRFIAQIAILELPSILTTGYSCVMHIHTAVEEVSFAKLLHKL 599
Query: 351 SKSTGELIKKKPRCLGNNSVAVVEIETSRPICIERYKDVKELGRVMLRVAGVTIAAGLVT 172
K T KK P + E+ET P+C+ER++D + +GR LR G T+A G V
Sbjct: 600 DK-TNRKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQGTTVAVGKVV 658
Query: 171 DII 163
I+
Sbjct: 659 KIL 661
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 82.2 bits (194), Expect = 7e-17
Identities = 44/189 (23%), Positives = 92/189 (48%), Gaps = 1/189 (0%)
Frame = -2
Query: 738 INDVFKGTGSGFCVSGRIENGVLKKGDKVLVCPTKEMAEVRSLSINDLTNNVAFAGDQVT 559
+ DV+K G G GR+E GV+K G V P EV+S+ ++ + + GD V
Sbjct: 248 LQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVG 307
Query: 558 LTLSGVDMQNVSVGYILSDPIEQVSV-TTRFEARIVVFNVKVPITKGFPVLVHYQSLVES 382
+ V ++++ G + D + F A++++ N I+ G+ ++ + +
Sbjct: 308 FNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIA 367
Query: 381 ANIVKLKALLSKSTGELIKKKPRCLGNNSVAVVEIETSRPICIERYKDVKELGRVMLRVA 202
+L + + +G+ I++ P+ + + + ++ S+P+C+E + D LGR +R
Sbjct: 368 CKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDM 427
Query: 201 GVTIAAGLV 175
T+A G++
Sbjct: 428 RQTVAVGVI 436
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 82.2 bits (194), Expect = 7e-17
Identities = 44/189 (23%), Positives = 92/189 (48%), Gaps = 1/189 (0%)
Frame = -2
Query: 738 INDVFKGTGSGFCVSGRIENGVLKKGDKVLVCPTKEMAEVRSLSINDLTNNVAFAGDQVT 559
+ DV+K G G GR+E GV+K G V P EV+S+ ++ + + GD V
Sbjct: 248 LQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVG 307
Query: 558 LTLSGVDMQNVSVGYILSDPIEQVSV-TTRFEARIVVFNVKVPITKGFPVLVHYQSLVES 382
+ V ++++ G + D + F A++++ N I+ G+ ++ + +
Sbjct: 308 FNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIA 367
Query: 381 ANIVKLKALLSKSTGELIKKKPRCLGNNSVAVVEIETSRPICIERYKDVKELGRVMLRVA 202
+L + + +G+ I++ P+ + + + ++ S+P+C+E + D LGR +R
Sbjct: 368 CKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDM 427
Query: 201 GVTIAAGLV 175
T+A G++
Sbjct: 428 RQTVAVGVI 436
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 82.2 bits (194), Expect = 7e-17
Identities = 44/189 (23%), Positives = 92/189 (48%), Gaps = 1/189 (0%)
Frame = -2
Query: 738 INDVFKGTGSGFCVSGRIENGVLKKGDKVLVCPTKEMAEVRSLSINDLTNNVAFAGDQVT 559
+ DV+K G G GR+E GV+K G V P EV+S+ ++ + + GD V
Sbjct: 248 LQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVG 307
Query: 558 LTLSGVDMQNVSVGYILSDPIEQVSV-TTRFEARIVVFNVKVPITKGFPVLVHYQSLVES 382
+ V ++++ G + D + F A++++ N I+ G+ ++ + +
Sbjct: 308 FNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIA 367
Query: 381 ANIVKLKALLSKSTGELIKKKPRCLGNNSVAVVEIETSRPICIERYKDVKELGRVMLRVA 202
+L + + +G+ I++ P+ + + + ++ S+P+C+E + D LGR +R
Sbjct: 368 CKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDM 427
Query: 201 GVTIAAGLV 175
T+A G++
Sbjct: 428 RQTVAVGVI 436
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 71.3 bits (167), Expect = 1e-13
Identities = 56/192 (29%), Positives = 103/192 (53%), Gaps = 1/192 (0%)
Frame = -2
Query: 738 INDVFKGTGSGFCVSGRIENGVLKKGDKVLVCPTKEMAEVRSLSIN-DLTNNVAFAGDQV 562
I+DV++ S V+GR+E G ++ + ++E A V+++ N D ++ A AGD V
Sbjct: 409 IDDVYRSPRS-VTVTGRVEAGNVQVNQVLYDVSSQEDAYVKNVIRNSDPSSTWAVAGDTV 467
Query: 561 TLTLSGVDMQNVSVGYILSDPIEQVSVTTRFEARIVVFNVKVPITKGFPVLVHYQSLVES 382
TL L+ +++ + G ILS+ V F A I F++ PI G +++H V S
Sbjct: 468 TLQLADIEVNQLRPGDILSNYENPVRRVRSFVAEIQTFDIHGPILSGSTLVLHLGRTVTS 527
Query: 381 ANIVKLKALLSKSTGELIKKKPRCLGNNSVAVVEIETSRPICIERYKDVKELGRVMLRVA 202
++ K+ + +K + + +K R L V + ++ P+C+ ++ LGR +LR +
Sbjct: 528 VSL-KIVTVNNKRSRHIASRK-RAL----VRISFLDGLFPLCLA--EECPALGRFILRRS 579
Query: 201 GVTIAAGLVTDI 166
G T+AAG+V ++
Sbjct: 580 GDTVAAGIVKEL 591
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 41.9 bits (94), Expect = 9e-05
Identities = 25/82 (30%), Positives = 40/82 (48%), Gaps = 2/82 (2%)
Frame = -2
Query: 738 INDVFKGTGSGFCVSGRIENGVLKKGDKVLVC--PTKEMAEVRSLSINDLTNNVAFAGDQ 565
I DVF +G G V+GR+E G LKKG ++ + + V + + + A AGD
Sbjct: 257 IEDVFSISGRGTVVTGRVERGTLKKGAEIEIVGYGSHLKTTVTGIEMFKKQLDAAVAGDN 316
Query: 564 VTLTLSGVDMQNVSVGYILSDP 499
L L + + + G I++ P
Sbjct: 317 CGLLLRSIKREQLKRGMIVAQP 338
>SPAC11E3.02c |||C2 domain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1237
Score = 26.6 bits (56), Expect = 3.7
Identities = 13/35 (37%), Positives = 18/35 (51%)
Frame = -3
Query: 140 DYYNLNIQLKDLLLIEDVIN*KYGYVWTF*LCTRE 36
DY++LN + L +DV YVW +CT E
Sbjct: 1022 DYFDLNFSIIAKHLTKDVFVTVMSYVWDEVVCTTE 1056
>SPAC56F8.03 |||translation initiation factor IF2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1079
Score = 25.8 bits (54), Expect = 6.4
Identities = 9/24 (37%), Positives = 16/24 (66%)
Frame = -2
Query: 714 GSGFCVSGRIENGVLKKGDKVLVC 643
G G + + NGVL +GD++++C
Sbjct: 721 GLGATIDVILSNGVLHEGDRIVLC 744
>SPAC20G8.10c ||SPAC3A12.01c|beclin family
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 464
Score = 25.8 bits (54), Expect = 6.4
Identities = 13/44 (29%), Positives = 20/44 (45%)
Frame = +2
Query: 560 VTWSPANATLLVRSFILKLLTSAISFVGQTNTLSPFFRTPFSMR 691
V W+ NA + +L +LT + F + L PF F +R
Sbjct: 312 VNWAEINAAWGMTVLLLDVLTEKLDFHSSSYQLKPFGSQSFIIR 355
>SPBC11B10.02c |his3||histidinol-phosphate aminotransferase
imidazole acetol phosphate transaminase
His3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 384
Score = 25.8 bits (54), Expect = 6.4
Identities = 13/47 (27%), Positives = 21/47 (44%)
Frame = -2
Query: 735 NDVFKGTGSGFCVSGRIENGVLKKGDKVLVCPTKEMAEVRSLSINDL 595
+++ G GS + I + DK+L+CP S IND+
Sbjct: 81 DNICMGVGSDEIIDSLIRISCIPGKDKILMCPPSYGMYTVSAKINDV 127
>SPBC23E6.01c ||SPBPJ758.01|RNA-binding protein, rrm
type|Schizosaccharomyces pombe|chr 2|||Manual
Length = 473
Score = 25.8 bits (54), Expect = 6.4
Identities = 10/27 (37%), Positives = 15/27 (55%)
Frame = -1
Query: 493 AGFCYNTFRGPNSSFQC*STNNKRLSG 413
AG+C+ F P+ + S NNK + G
Sbjct: 134 AGYCFVEFASPHEASSAMSMNNKPIPG 160
>SPBC19G7.01c |msh2|swi8, mut3, SPBC24C6.12c|MutS protein homolog
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 982
Score = 25.8 bits (54), Expect = 6.4
Identities = 17/52 (32%), Positives = 28/52 (53%)
Frame = -2
Query: 387 ESANIVKLKALLSKSTGELIKKKPRCLGNNSVAVVEIETSRPICIERYKDVK 232
+S+NI+ + +L E I+ R L NN A + ++ +R C+E DVK
Sbjct: 653 QSSNILDIVSLEDTPNFEEIR---RTLENNHCARLYLKQARHPCLEAQDDVK 701
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 25.4 bits (53), Expect = 8.5
Identities = 18/61 (29%), Positives = 26/61 (42%), Gaps = 3/61 (4%)
Frame = +2
Query: 500 GSLSMYPTDTFCMSTPDKVN---VTWSPANATLLVRSFILKLLTSAISFVGQTNTLSPFF 670
G +SM D S K + V W P N V S K L + +F+G + ++ F
Sbjct: 319 GKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVLKAVCSVPPKDLKMSATFIGNSTSIQEIF 378
Query: 671 R 673
R
Sbjct: 379 R 379
>SPCC330.07c |||membrane transporter|Schizosaccharomyces pombe|chr
3|||Manual
Length = 500
Score = 25.4 bits (53), Expect = 8.5
Identities = 21/66 (31%), Positives = 29/66 (43%), Gaps = 5/66 (7%)
Frame = +2
Query: 479 VTETCSIGSLSM---YPTDTFCMSTPDKVNVTWSPANATLLVRSFILKLLTSA--ISFVG 643
VTET S+ YP D P KV++ + N+ + S + L TSA +G
Sbjct: 59 VTETFQSNRSSLVPTYPVDENANKLPPKVSIAFILINSLMSDMSLAVALPTSASYTQSLG 118
Query: 644 QTNTLS 661
TN S
Sbjct: 119 GTNAFS 124
>SPAC3G9.13c |msw1||mitochondrial tryptophan-tRNA ligase Msw1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 361
Score = 25.4 bits (53), Expect = 8.5
Identities = 13/32 (40%), Positives = 19/32 (59%)
Frame = -2
Query: 357 LLSKSTGELIKKKPRCLGNNSVAVVEIETSRP 262
LLS STGE+ KK R +N + +++RP
Sbjct: 238 LLSDSTGEIRKKISRAQTDNIKGITYGDSNRP 269
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,834,800
Number of Sequences: 5004
Number of extensions: 54479
Number of successful extensions: 184
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 173
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 178
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 349251756
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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