Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner11h14f
(598 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 47 2e-06
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 44 2e-05
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 36 0.006
SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 32 0.073
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 31 0.13
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 30 0.29
SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 29 0.39
SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizo... 27 1.6
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 27 1.6
SPAC57A10.05c |pof1||F-box protein Pof1|Schizosaccharomyces pomb... 27 2.1
SPCC645.09 |mrpl37||mitochondrial ribosomal protein subunit L37|... 27 2.7
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 26 3.6
SPCC613.04c |rng3||UCS-domain protein |Schizosaccharomyces pombe... 26 4.8
SPCC63.04 |mok14||alpha-1,3-glucan synthase Mok14|Schizosaccharo... 26 4.8
SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc... 25 6.3
SPAC2C4.17c |||MS ion channel protein 2|Schizosaccharomyces pomb... 25 6.3
SPAC16A10.07c |taz1|myb1, myb|human TRF ortholog Taz1|Schizosacc... 25 6.3
SPBC337.07c |||carboxypeptidase |Schizosaccharomyces pombe|chr 2... 25 6.3
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 25 8.4
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 46.8 bits (106), Expect = 2e-06
Identities = 25/89 (28%), Positives = 46/89 (51%), Gaps = 1/89 (1%)
Frame = +3
Query: 321 DDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFLIKIRPPMSES 500
D DG+ + E ++ G +E +++ ++ D D +G+I EFL + M ++
Sbjct: 24 DQDGN--ITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLTMMARKMKDT 81
Query: 501 -RRNIVEQAFKKLDKTGDGAITIDDIKGV 584
V +AFK DK G+G IT++++ V
Sbjct: 82 DNEEEVREAFKVFDKDGNGYITVEELTHV 110
Score = 37.1 bits (82), Expect = 0.002
Identities = 16/53 (30%), Positives = 28/53 (52%)
Frame = +3
Query: 309 FRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEF 467
F+ D DG+ + EE + + G L++ E ++ + DTD G I+ +EF
Sbjct: 91 FKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEF 143
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 44.0 bits (99), Expect = 2e-05
Identities = 32/139 (23%), Positives = 65/139 (46%), Gaps = 6/139 (4%)
Frame = +3
Query: 192 EELMQKSARAMTQATDPLEKLRLLCLSRGASGILGLGRIFRRMDDDGSKLLNKEEFLYGI 371
E + ++ + + +++ L + AS L R+F +D+DG ++ +EF+ +
Sbjct: 24 ERIRKRFIKIDANQSGSIDRNEFLSIPSVASNPLA-SRLFSVVDEDGGGDVDFQEFINSL 82
Query: 372 KETGLELNKSEAEEL-FSQFDTDSSGSISLDEFLIKIRPPMSESRR-----NIVEQAFKK 533
+ NK E + F +D D G IS E + ++ + + R IV++ +
Sbjct: 83 SVFSVHGNKEEKLKFAFKIYDIDRDGYISNGELYLVLKMMVGTNLREDQLQQIVDKTIME 142
Query: 534 LDKTGDGAITIDDIKGVYS 590
+DK DG I+ ++ K + S
Sbjct: 143 VDKDRDGKISFEEFKDIVS 161
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 35.5 bits (78), Expect = 0.006
Identities = 18/97 (18%), Positives = 42/97 (43%), Gaps = 1/97 (1%)
Frame = +3
Query: 297 LGRIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFL-I 473
+ F+ D D ++ E ++ G KSE ++ FD G + +++F+ +
Sbjct: 39 INEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRV 98
Query: 474 KIRPPMSESRRNIVEQAFKKLDKTGDGAITIDDIKGV 584
+ +++AF+ D G I++ +++ V
Sbjct: 99 MTEKIVERDPLEEIKRAFELFDDDETGKISLRNLRRV 135
Score = 33.1 bits (72), Expect = 0.032
Identities = 16/56 (28%), Positives = 25/56 (44%)
Frame = +3
Query: 303 RIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFL 470
R F DDD + ++ KE ++ E E + +FD D G I+ EF+
Sbjct: 114 RAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFDLDQDGEINEQEFI 169
Score = 32.7 bits (71), Expect = 0.042
Identities = 16/62 (25%), Positives = 29/62 (46%)
Frame = +3
Query: 387 ELNKSEAEELFSQFDTDSSGSISLDEFLIKIRPPMSESRRNIVEQAFKKLDKTGDGAITI 566
E + + E F FD+D +I E +R + ++ V + + DKTG G + +
Sbjct: 33 EEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQM 92
Query: 567 DD 572
+D
Sbjct: 93 ED 94
>SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 621
Score = 31.9 bits (69), Expect = 0.073
Identities = 22/96 (22%), Positives = 40/96 (41%), Gaps = 2/96 (2%)
Frame = +3
Query: 294 GLGRIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFLI 473
G+ ++FR + S +LN+ EF + GL + E LF + G ++ + F
Sbjct: 488 GITKVFRHFEKKKSNMLNEVEFYAALASLGLVYDTEEGTALFHRAANSEEG-VTYERFTE 546
Query: 474 KIRPPMSESRRNIVEQAFKKLDKTGDGA--ITIDDI 575
+ + + R+ Q DG +T DD+
Sbjct: 547 IVMEELED--RDSARQVLYAFCDVADGKSYVTSDDL 580
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 31.1 bits (67), Expect = 0.13
Identities = 23/79 (29%), Positives = 37/79 (46%), Gaps = 1/79 (1%)
Frame = +3
Query: 342 LNKEEFLYGIKETGLELNKSE-AEELFSQFDTDSSGSISLDEFLIKIRPPMSESRRNIVE 518
LNK EF K+ + S AE +F+ FD D +G I EF+ + + +
Sbjct: 43 LNKSEFQKIYKQFFPFGDPSAFAEYVFNVFDADKNGYIDFKEFICALSVTSRGELNDKLI 102
Query: 519 QAFKKLDKTGDGAITIDDI 575
AF+ D +G I+ D++
Sbjct: 103 WAFQLYDLDNNGLISYDEM 121
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 29.9 bits (64), Expect = 0.29
Identities = 18/74 (24%), Positives = 31/74 (41%)
Frame = +3
Query: 354 EFLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFLIKIRPPMSESRRNIVEQAFKK 533
E+ G+ + + S LF +FD +GS+SL + + I + F+
Sbjct: 564 EWAKGLDAAAINNSSSFLRHLFLRFDKSMTGSLSLQDLVSGIAELKFRDVMRNISFIFEL 623
Query: 534 LDKTGDGAITIDDI 575
D GDG + D+
Sbjct: 624 YDFNGDGFMDKPDV 637
>SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1044
Score = 29.5 bits (63), Expect = 0.39
Identities = 18/58 (31%), Positives = 29/58 (50%), Gaps = 1/58 (1%)
Frame = +1
Query: 397 KVKPKNSSVNSTQTVVAQSVLMNSLLKSVLPCRNRVVT-L*NKHSRSLTRLVTVQSQL 567
K+ KN+ S + + ++ L L L + +++ L NK S TRL +QSQL
Sbjct: 765 KLNKKNADTESFKNTIREAELSKKALNDNLGNKENIISDLKNKLSEESTRLQELQSQL 822
>SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1336
Score = 27.5 bits (58), Expect = 1.6
Identities = 22/71 (30%), Positives = 32/71 (45%)
Frame = +3
Query: 357 FLYGIKETGLELNKSEAEELFSQFDTDSSGSISLDEFLIKIRPPMSESRRNIVEQAFKKL 536
F+ +E+ LE E FSQ +S + SL+ I P SRR IVEQ ++
Sbjct: 680 FILPNEESLLEKYWINYNESFSQLSRESLFT-SLESPFTDIESPTIVSRRKIVEQRKLRM 738
Query: 537 DKTGDGAITID 569
+K +D
Sbjct: 739 EKESFQETNVD 749
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 27.5 bits (58), Expect = 1.6
Identities = 12/30 (40%), Positives = 20/30 (66%)
Frame = +3
Query: 489 MSESRRNIVEQAFKKLDKTGDGAITIDDIK 578
++ S+ +++AF LDK GDG I +D+K
Sbjct: 42 LTSSQIQELKEAFALLDKDGDGNIGREDVK 71
>SPAC57A10.05c |pof1||F-box protein Pof1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 605
Score = 27.1 bits (57), Expect = 2.1
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = +2
Query: 23 TGDNCVRSWCFSGGKRRGAEGYQRPLLQLR 112
+ D V+ W FSGGKR G+ P+ +R
Sbjct: 370 SADCTVKLWHFSGGKRITLRGHTGPVNSVR 399
>SPCC645.09 |mrpl37||mitochondrial ribosomal protein subunit
L37|Schizosaccharomyces pombe|chr 3|||Manual
Length = 139
Score = 26.6 bits (56), Expect = 2.7
Identities = 16/48 (33%), Positives = 23/48 (47%)
Frame = +1
Query: 346 TKKNSFMASRKQGWNLIKVKPKNSSVNSTQTVVAQSVLMNSLLKSVLP 489
+ +NS + K+ + +V PK NS T AQ V + SVLP
Sbjct: 32 SSRNSSSSLVKRSYVSSRVSPKKPQHNSDATSSAQKVANKTHTSSVLP 79
>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
Plc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 899
Score = 26.2 bits (55), Expect = 3.6
Identities = 14/30 (46%), Positives = 17/30 (56%), Gaps = 2/30 (6%)
Frame = +3
Query: 384 LELNKSEA--EELFSQFDTDSSGSISLDEF 467
L LN S EE F + D D SG +S +EF
Sbjct: 326 LHLNASMEFLEETFQKADADHSGKLSFEEF 355
>SPCC613.04c |rng3||UCS-domain protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 746
Score = 25.8 bits (54), Expect = 4.8
Identities = 10/34 (29%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
Frame = -2
Query: 366 HKGIL-LCSVVYFHHHPCDGKFFQVPRSLKHLGT 268
H+G++ +C++VY KF + P++++ L T
Sbjct: 686 HRGLVCICNIVYSKDQEIFNKFIKTPKAVETLRT 719
>SPCC63.04 |mok14||alpha-1,3-glucan synthase Mok14|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1369
Score = 25.8 bits (54), Expect = 4.8
Identities = 24/99 (24%), Positives = 43/99 (43%)
Frame = +3
Query: 237 DPLEKLRLLCLSRGASGILGLGRIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEEL 416
D E+ + S +S + L I + GS+L E F I G+ + AE
Sbjct: 793 DNYEQAESIISSLSSSALSELSYISESSMNIGSRL--DERF---IDANGVAIRDFSAELT 847
Query: 417 FSQFDTDSSGSISLDEFLIKIRPPMSESRRNIVEQAFKK 533
+ + +S G +S+D FL K++ + + + F+K
Sbjct: 848 YLTPE-NSKGKLSIDHFLNKVQSRWHDEEHHYYKTGFRK 885
>SPAC688.08 |srb8|med12|mediator complex subunit Srb8
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1233
Score = 25.4 bits (53), Expect = 6.3
Identities = 10/34 (29%), Positives = 17/34 (50%)
Frame = -3
Query: 560 DCTVTSLVKLLECLFYNVTTRFRHGRTDFNKEFI 459
D TV + +E LF+N+T +H F + +
Sbjct: 699 DATVANRKDFIEFLFHNITVSSKHTAVIFTSDLL 732
>SPAC2C4.17c |||MS ion channel protein 2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 840
Score = 25.4 bits (53), Expect = 6.3
Identities = 19/60 (31%), Positives = 27/60 (45%), Gaps = 5/60 (8%)
Frame = +3
Query: 402 EAEELFSQFDTDSSGSISLDEF-----LIKIRPPMSESRRNIVEQAFKKLDKTGDGAITI 566
E ++F D D S +++LDE I I S V+ A KLD+ G G + I
Sbjct: 396 EINDIFHILDNDYSRTVTLDEMEQFTREISIEFRSISSSLRDVDLALGKLDRVGLGVVGI 455
>SPAC16A10.07c |taz1|myb1, myb|human TRF ortholog
Taz1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 663
Score = 25.4 bits (53), Expect = 6.3
Identities = 13/36 (36%), Positives = 18/36 (50%)
Frame = +3
Query: 408 EELFSQFDTDSSGSISLDEFLIKIRPPMSESRRNIV 515
E + F TDS+ SI EF++ P S N+V
Sbjct: 273 ELVIRYFGTDSNPSIDASEFILSCLPYTSLDALNVV 308
>SPBC337.07c |||carboxypeptidase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 497
Score = 25.4 bits (53), Expect = 6.3
Identities = 25/101 (24%), Positives = 43/101 (42%), Gaps = 5/101 (4%)
Frame = +3
Query: 180 SVQEEELMQKSARAMTQATDPLEKLRLLCLSRGASGILGLGRIFRRMDDDGSKLLNKEEF 359
S Q E + R M L +L + ++ ILGL R D+G K+ NK+
Sbjct: 182 SYQNLESINSWLRLMASLYKDLSELVPVGITAEGRTILGLKLNGRHPSDNGEKIRNKKVI 241
Query: 360 L-----YGIKETGLELNKSEAEELFSQFDTDSSGSISLDEF 467
+ + + G+ A +L +++D+D LD+F
Sbjct: 242 IIQGGSHAREWIGIPSVCYAAWQLLAKYDSDGHVRKLLDKF 282
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 25.0 bits (52), Expect = 8.4
Identities = 13/36 (36%), Positives = 21/36 (58%), Gaps = 1/36 (2%)
Frame = +3
Query: 306 IFRRMDDDGSKLLNKEEFLYGIKETGL-ELNKSEAE 410
IFRR+ D S + ++ FL+ G+ E+ +EAE
Sbjct: 377 IFRRLGDQFSAMFRRKAFLHWYTGEGMDEMEFTEAE 412
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,390,472
Number of Sequences: 5004
Number of extensions: 48126
Number of successful extensions: 190
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 176
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 188
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 260219058
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -