Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner11c20f
(588 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC36B7.04 |||tRNA dihydrouridine synthase Dus1 |Schizosaccharo... 205 4e-54
SPCC777.15 |||tRNA dihydrouridine synthase Dus4 |Schizosaccharom... 99 4e-22
SPBC1709.06 |dus2||tRNA dihydrouridine synthase Dus2 |Schizosacc... 62 8e-11
SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha... 36 0.004
SPAC1786.03 |cut11|SPAC24C9.01|integral membrane nucleoporin|Sch... 29 0.38
SPAC19E9.02 |fin1||serine/threonine protein kinase Fin1|Schizosa... 29 0.50
SPCC1672.11c |||P-type ATPase |Schizosaccharomyces pombe|chr 3||... 27 2.7
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 27 2.7
SPAC27E2.07 |pvg2|mug53|galactose residue biosynthesis protein P... 26 3.5
SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr 3|||Ma... 26 4.7
SPAC17A2.09c |csx1||RNA-binding protein Csx1|Schizosaccharomyces... 26 4.7
SPBC19C7.11 |||ClC chloride channel |Schizosaccharomyces pombe|c... 25 6.2
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 25 8.2
SPAC1A6.09c |lag1||sphingosine N-acyltransferase Lag1|Schizosacc... 25 8.2
>SPBC36B7.04 |||tRNA dihydrouridine synthase Dus1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 399
Score = 205 bits (500), Expect = 4e-54
Identities = 88/163 (53%), Positives = 122/163 (74%), Gaps = 2/163 (1%)
Frame = +2
Query: 104 ELNNPQFVVAPMVDASELAWRLLCRRHGANLCYTPMLHSNVFTKDAKYRKD--NLVTCEE 277
++ P+ ++APMVD SEL WR+L RR GA+LCY+PM HS +F + YR + T E
Sbjct: 14 KIGRPKRILAPMVDQSELPWRILARRSGADLCYSPMFHSRLFGESEDYRNKVFSTRTIPE 73
Query: 278 DRPLIVQFCGNNPEIMASAAKLAEEYCDAIDINLGCPQSIAKRGRYGSFLQDDWELLKEI 457
+RPLI+QFCGN+PEIM AAK+A YCDA+D+NLGCPQ IAK+G+YGSFLQ++W L++ I
Sbjct: 74 ERPLIIQFCGNDPEIMLKAAKIAAPYCDAVDVNLGCPQGIAKKGKYGSFLQENWNLIESI 133
Query: 458 VTAMSKAVSIPITCKVRIFEDIDKSVNYAKMLEASGCKLLTVH 586
+T + +SIP+T K+RIF D K+++YAKM+ +G +L VH
Sbjct: 134 ITKLHTELSIPVTAKIRIFPDPQKTLDYAKMILKAGASILAVH 176
>SPCC777.15 |||tRNA dihydrouridine synthase Dus4
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 326
Score = 99.1 bits (236), Expect = 4e-22
Identities = 61/168 (36%), Positives = 96/168 (57%), Gaps = 5/168 (2%)
Frame = +2
Query: 98 IFELNN---PQFVVAPMVDASELAWRLLCRRHGANLCYTPMLHSNVFTKDAKYRKDNLVT 268
IFE+N P + APMV S+L +R L R + ++ YTPM+ + F K R + T
Sbjct: 11 IFEINKGKRPVHIAAPMVRYSKLPFRQLVRDYNTDIVYTPMILAKEFLHP-KGRYFDFST 69
Query: 269 CEEDRPLIVQFCGNNPEIMASAAKLAEEYCDAIDINLGCPQSIAKRGRYGSFLQDDWELL 448
+ D LI+QF ++P I+ AA+L Y D I IN GCPQ+ A + GS L D+ E +
Sbjct: 70 NDADASLILQFGVDDPVILEKAAQLVGPYVDGIGINCGCPQTWAIQEGIGSALLDEPEKV 129
Query: 449 KEIVTAMSKAV--SIPITCKVRIFEDIDKSVNYAKMLEASGCKLLTVH 586
++V A+ + S K+RI +D++K+ + +++E SG ++TVH
Sbjct: 130 HKLVRAVKSTLGESFCTEVKIRIAKDLNKTRHLMQVIEKSGADIITVH 177
>SPBC1709.06 |dus2||tRNA dihydrouridine synthase Dus2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 479
Score = 61.7 bits (143), Expect = 8e-11
Identities = 49/176 (27%), Positives = 77/176 (43%), Gaps = 23/176 (13%)
Frame = +2
Query: 128 VAPMVDASELAWRLLCRRHGANLCYTPMLHSNVFTKDAKYRK--DNLVTCE--------- 274
+APMV EL RLL R+GANL + P + + ++ + C
Sbjct: 12 LAPMVRIGELPMRLLALRYGANLVWGPEIVDKALLSGTPVERVVNDRINCIDFVKPPSNK 71
Query: 275 --------EDRPLIVQFCGNNPEIMASAAKLAEEYCDAIDINLGCPQSIAKRGRYGSFL- 427
E LI Q +PE+ AAKL ID+N GCP+ + G+ L
Sbjct: 72 VLFRVHPLEANRLIFQLGSASPELAVEAAKLVANDVAGIDLNCGCPKHFSVHAGMGAGLL 131
Query: 428 --QDDW-ELLKEIVTAMSKAVSIPITCKVRIFEDIDKSVNYAKMLEASGCKLLTVH 586
QD +L +V + K I I+CK+R+ E + ++ + + +G + +TVH
Sbjct: 132 KNQDRLVSILDALVNEIGKPYKISISCKIRLLETKEDTLKLVERICDTGVRAITVH 187
>SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 617
Score = 35.9 bits (79), Expect = 0.004
Identities = 21/71 (29%), Positives = 37/71 (52%), Gaps = 4/71 (5%)
Frame = +2
Query: 335 AKLAEEYCDAID---INLGCP-QSIAKRGRYGSFLQDDWELLKEIVTAMSKAVSIPITCK 502
A++ E CD +D +N GCP + ++G S L++ LL+ + + + IP+T K
Sbjct: 321 AQVIAEQCDGVDFLDLNCGCPIDLVFRQGAGSSLLENPGRLLRNLQGMDAVSGQIPVTVK 380
Query: 503 VRIFEDIDKSV 535
+R+ D V
Sbjct: 381 LRMGNKDDHPV 391
>SPAC1786.03 |cut11|SPAC24C9.01|integral membrane
nucleoporin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 601
Score = 29.5 bits (63), Expect = 0.38
Identities = 19/68 (27%), Positives = 32/68 (47%)
Frame = +2
Query: 353 YCDAIDINLGCPQSIAKRGRYGSFLQDDWELLKEIVTAMSKAVSIPITCKVRIFEDIDKS 532
YCD D+ + +S+ K + L DD++ L A+ + I IT K F+D
Sbjct: 524 YCDTFDVLVSYKRSLVKNHSNSTNLDDDFKNLNSAANALHCGI-IDITEK---FQDFFTQ 579
Query: 533 VNYAKMLE 556
+N + +E
Sbjct: 580 LNLSPRIE 587
>SPAC19E9.02 |fin1||serine/threonine protein kinase
Fin1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 722
Score = 29.1 bits (62), Expect = 0.50
Identities = 18/66 (27%), Positives = 28/66 (42%), Gaps = 1/66 (1%)
Frame = +2
Query: 179 RHGANLCYTPMLHSNVFTKDAKYRKDNL-VTCEEDRPLIVQFCGNNPEIMASAAKLAEEY 355
+ GA L + N+ ++ +Y D + + P IVQ+CG A L EY
Sbjct: 25 KDGALLAQKEIHFGNITRQEKQYIADEVNILRNLKHPNIVQYCGEELNRSAQVINLYMEY 84
Query: 356 CDAIDI 373
C D+
Sbjct: 85 CGHGDL 90
>SPCC1672.11c |||P-type ATPase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1315
Score = 26.6 bits (56), Expect = 2.7
Identities = 11/40 (27%), Positives = 23/40 (57%)
Frame = +2
Query: 191 NLCYTPMLHSNVFTKDAKYRKDNLVTCEEDRPLIVQFCGN 310
N+ + +L + +F + + K+ LV+C ++ V FCG+
Sbjct: 976 NVFHHILLKAQIFARMSPSEKNELVSCFQNLNYCVGFCGD 1015
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 26.6 bits (56), Expect = 2.7
Identities = 11/25 (44%), Positives = 16/25 (64%)
Frame = +2
Query: 353 YCDAIDINLGCPQSIAKRGRYGSFL 427
Y A+ I+LG P++ A+ GRY L
Sbjct: 2973 YAAAVQIDLGLPKAWAEWGRYNDLL 2997
>SPAC27E2.07 |pvg2|mug53|galactose residue biosynthesis protein
Pvg2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 389
Score = 26.2 bits (55), Expect = 3.5
Identities = 12/43 (27%), Positives = 18/43 (41%)
Frame = +2
Query: 74 NNYIMANWIFELNNPQFVVAPMVDASELAWRLLCRRHGANLCY 202
N YI +F NNP + P +A W L + +C+
Sbjct: 312 NTYIDDGKVFVKNNPHIIFIP-TEAFASTWDFLPSEESSEMCF 353
>SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 630
Score = 25.8 bits (54), Expect = 4.7
Identities = 15/51 (29%), Positives = 23/51 (45%), Gaps = 2/51 (3%)
Frame = +2
Query: 437 WELLKEIVTAMSKAVSIPITCKVRIFEDIDKSVNY--AKMLEASGCKLLTV 583
W KE ++++ P + +RI E+ NY L A G LL+V
Sbjct: 563 WNTSKEFFIQLAESAQYPASSIIRIPEEDSNKTNYQLVAALTAFGALLLSV 613
>SPAC17A2.09c |csx1||RNA-binding protein Csx1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 632
Score = 25.8 bits (54), Expect = 4.7
Identities = 18/60 (30%), Positives = 31/60 (51%)
Frame = -1
Query: 387 GHPRLMSIASQYSSANFAADAIISGLLPQNCTIKGRSSSQVTRLSFLYLASFVKTLLCNI 208
G P +S+AS S A+ AAD+ + G++P + T + + + + L FV L N+
Sbjct: 251 GRPLRISVASPKSRASIAADSAL-GIVPTS-TSNRQPNQDLCSMDPLNTTVFVGGLASNL 308
>SPBC19C7.11 |||ClC chloride channel |Schizosaccharomyces pombe|chr
2|||Manual
Length = 812
Score = 25.4 bits (53), Expect = 6.2
Identities = 8/21 (38%), Positives = 12/21 (57%)
Frame = -3
Query: 511 YSHFTCDWYGNSLGHCSHDLF 449
Y+ C WY ++G HDL+
Sbjct: 136 YNEKFCCWYSETMGMFKHDLY 156
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 25.0 bits (52), Expect = 8.2
Identities = 12/37 (32%), Positives = 21/37 (56%), Gaps = 4/37 (10%)
Frame = +2
Query: 263 VTCEEDRPLIVQF----CGNNPEIMASAAKLAEEYCD 361
+ +E + ++V+F CG+ + + KLAEEY D
Sbjct: 368 IVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEEYSD 404
>SPAC1A6.09c |lag1||sphingosine N-acyltransferase
Lag1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 25.0 bits (52), Expect = 8.2
Identities = 9/24 (37%), Positives = 18/24 (75%)
Frame = -1
Query: 114 LFNSKIQLAIM*LLFTRRIIIFLY 43
+FN I +A + LLFT +++I+++
Sbjct: 329 IFNKPIYIAFIILLFTLQLLIYIW 352
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,408,108
Number of Sequences: 5004
Number of extensions: 48612
Number of successful extensions: 115
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 106
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 111
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 254167452
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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