BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner11a04r
(737 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC947.01 |||AAA family ATPase, unknown biological role|Schizos... 31 0.23
SPCC1259.13 |chk1|rad27|Chk1 protein kinase|Schizosaccharomyces ... 30 0.40
SPCC736.11 |ago1|csp9|argonaute|Schizosaccharomyces pombe|chr 3|... 28 1.2
SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces... 28 1.6
SPAC2F3.09 |hem1||5-aminolevulinate synthase|Schizosaccharomyces... 27 3.7
SPAC644.13c |||Rab GTPase binding |Schizosaccharomyces pombe|chr... 27 3.7
SPCC61.05 |||S. pombe specific multicopy membrane protein family... 26 6.4
SPBP23A10.11c |||conserved fungal protein|Schizosaccharomyces po... 25 8.5
>SPBC947.01 |||AAA family ATPase, unknown biological
role|Schizosaccharomyces pombe|chr 2|||Manual
Length = 660
Score = 30.7 bits (66), Expect = 0.23
Identities = 21/87 (24%), Positives = 47/87 (54%)
Frame = +2
Query: 383 SMMSFLDLSAQNIRAAKMRVIDHTGRRASVNVSTFARRFFLAK*TLYTATGAVPNKPTR* 562
S S + ++++ A+ + TGR A++N +TF T T + +V + P+
Sbjct: 191 SSASAIAAASKSAAASASALSSDTGRSATMNSTTFPTAMKSQSTTKPTLSNSV-SSPSIQ 249
Query: 563 INTSKNKANDTSPYTWNLTSPGVYSPA 643
++ ++N AN+++P +++ P ++ PA
Sbjct: 250 VSNNQN-ANNSTPLSFHAPIPPLHVPA 275
>SPCC1259.13 |chk1|rad27|Chk1 protein kinase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 496
Score = 29.9 bits (64), Expect = 0.40
Identities = 20/54 (37%), Positives = 25/54 (46%)
Frame = +2
Query: 575 KNKANDTSPYTWNLTSPGVYSPATMKIIEEVHMLTS*PQGKYNVPVLYFSQWLT 736
K + S + WNL SPG YS T ML S P +Y+V + WLT
Sbjct: 227 KKQCERPSYHPWNLLSPGAYSIIT-------GMLRSDPFKRYSVKHVVQHPWLT 273
>SPCC736.11 |ago1|csp9|argonaute|Schizosaccharomyces pombe|chr
3|||Manual
Length = 834
Score = 28.3 bits (60), Expect = 1.2
Identities = 9/20 (45%), Positives = 14/20 (70%)
Frame = -2
Query: 478 DINGSPTPGVVNDPHLSRPY 419
D NG+P PG + + H++ PY
Sbjct: 706 DRNGNPLPGTIIEKHVTHPY 725
>SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 199
Score = 27.9 bits (59), Expect = 1.6
Identities = 17/59 (28%), Positives = 30/59 (50%), Gaps = 2/59 (3%)
Frame = -3
Query: 684 YDVSMWTSSMIFIVAGLYTPGLVKFHVYGD-VSFALF-FEVLIHLVGLLGTAPVAVYNV 514
+ S+WT++++ I+ GLY + FH G+ F F + + GLLG A + +
Sbjct: 4 FSTSLWTTTVMSIIVGLY----MLFHNSGESFDFGSFLLDTSPYTWGLLGIASCVAFGI 58
>SPAC2F3.09 |hem1||5-aminolevulinate synthase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 558
Score = 26.6 bits (56), Expect = 3.7
Identities = 11/23 (47%), Positives = 16/23 (69%)
Frame = +1
Query: 19 HLEKYYTYFNNISNKIIVKKFPL 87
H +K Y YFNNI+ + K++PL
Sbjct: 146 HRDKSYRYFNNINR--LAKEYPL 166
>SPAC644.13c |||Rab GTPase binding |Schizosaccharomyces pombe|chr
1|||Manual
Length = 225
Score = 26.6 bits (56), Expect = 3.7
Identities = 10/19 (52%), Positives = 15/19 (78%)
Frame = +1
Query: 46 NNISNKIIVKKFPLFLFLF 102
+N+SNK ++ +PLFLF F
Sbjct: 199 SNLSNKKLLAVYPLFLFYF 217
>SPCC61.05 |||S. pombe specific multicopy membrane protein family
1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 469
Score = 25.8 bits (54), Expect = 6.4
Identities = 18/60 (30%), Positives = 30/60 (50%), Gaps = 2/60 (3%)
Frame = -3
Query: 687 GYDVSMWTSSMIFIVAGL--YTPGLVKFHVYGDVSFALFFEVLIHLVGLLGTAPVAVYNV 514
G + S +TS ++ + GL P LVK++VY ++F F + L L ++ Y V
Sbjct: 278 GLEQSAYTSFLLVLCFGLGITKPALVKYYVY--LAFVAFVQGLFVTFAPLSYPVMSFYGV 335
>SPBP23A10.11c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 507
Score = 25.4 bits (53), Expect = 8.5
Identities = 10/41 (24%), Positives = 19/41 (46%)
Frame = -3
Query: 681 DVSMWTSSMIFIVAGLYTPGLVKFHVYGDVSFALFFEVLIH 559
+ S+WT++ G Y PG+ +H + L ++ H
Sbjct: 332 EFSIWTATECNNDCGYYLPGIPAYHGFSGAKLVLMEFIMPH 372
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,190,187
Number of Sequences: 5004
Number of extensions: 68371
Number of successful extensions: 198
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 185
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 198
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 349251756
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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