BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner11a04f
(635 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC22A12.10 |||diacylglycerol cholinephosphotranferase/ diacylg... 99 5e-22
SPCC622.11 |||LMBR1-like membrane protein|Schizosaccharomyces po... 29 0.43
SPCC613.01 ||SPCC757.14|membrane transporter|Schizosaccharomyces... 27 1.7
SPBC21D10.11c |nfs1||iron-sulfur cluster assembly protein Nfs1|S... 26 5.2
SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces pomb... 25 9.1
SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce... 25 9.1
>SPAC22A12.10 |||diacylglycerol cholinephosphotranferase/
diacylglycerol ethanolaminesphotranferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 386
Score = 99.1 bits (236), Expect = 5e-22
Identities = 53/128 (41%), Positives = 74/128 (57%), Gaps = 1/128 (0%)
Frame = +3
Query: 255 LSQNHFDGLKKYKYSAIDTSPLSNYVMHPSW-NFITRFIPKWIAPNLITFAGFVCMVIVI 431
L++ L YKYSAID S LS Y++ P W N + + IP +APNLIT G +VI I
Sbjct: 3 LNRKQLKNLHNYKYSAIDNSLLSKYILKPYWWNQLLKVIPMSMAPNLITLIGLGFVVINI 62
Query: 432 LLLTIFDYDFHGAERLRQGVDEYRIPNWALMACGVLLFLAYNLDGIDGKQARKLGVSGPL 611
L + ++ Y + +D + P+W + + LFL + D IDG QAR+ G S PL
Sbjct: 63 LTMLVYKYHYE--------MDAF--PSWVYASWAIGLFLYQSFDAIDGSQARRTGTSSPL 112
Query: 612 GELFDHGL 635
G+LFDHG+
Sbjct: 113 GQLFDHGV 120
>SPCC622.11 |||LMBR1-like membrane protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 562
Score = 29.5 bits (63), Expect = 0.43
Identities = 19/63 (30%), Positives = 31/63 (49%)
Frame = -2
Query: 232 SMVVIRFLQILTRLQGQKMLVIFRNKNKLLFSFVTFDLNTSLYANLTFFRI*TLLFCIVM 53
S+ + RFL LT + +L + + FS L+ S Y NL ++ + T L C+V+
Sbjct: 116 SLSIWRFLYWLTFVLSWIILPFVQGYMESKFSTPRSRLSDSFYKNLRYYLLLTFLTCVVI 175
Query: 52 IIL 44
L
Sbjct: 176 AYL 178
>SPCC613.01 ||SPCC757.14|membrane transporter|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 497
Score = 27.5 bits (58), Expect = 1.7
Identities = 12/45 (26%), Positives = 25/45 (55%)
Frame = +3
Query: 318 LSNYVMHPSWNFITRFIPKWIAPNLITFAGFVCMVIVILLLTIFD 452
LS+ V+ P + F + F+ KW+ I GF+ ++ +++ + D
Sbjct: 353 LSSLVIAP-FVFFSTFLSKWLEDRQIMLYGFMMGIVALIVHLVLD 396
>SPBC21D10.11c |nfs1||iron-sulfur cluster assembly protein
Nfs1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 498
Score = 25.8 bits (54), Expect = 5.2
Identities = 16/52 (30%), Positives = 28/52 (53%), Gaps = 5/52 (9%)
Frame = +3
Query: 216 RITTMEKLFEYKY---LSQNHFDGLKKYKYSAIDTSPLSNY--VMHPSWNFI 356
RI E ++Y + LSQ DGL Y++++ P S Y ++ S+N++
Sbjct: 351 RICKEEMKYDYAHISKLSQRLIDGLLAIPYTSLNGDPKSRYPGCVNISFNYV 402
>SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 331
Score = 25.0 bits (52), Expect = 9.1
Identities = 12/29 (41%), Positives = 19/29 (65%), Gaps = 1/29 (3%)
Frame = +3
Query: 309 TSPLSNYVMHPSWNFIT-RFIPKWIAPNL 392
TSPL +Y +N ++ +F+PKW +P L
Sbjct: 225 TSPL-DYTAKVQFNRLSGKFMPKWASPEL 252
>SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2100
Score = 25.0 bits (52), Expect = 9.1
Identities = 9/17 (52%), Positives = 13/17 (76%)
Frame = +3
Query: 18 LYESACSVNKIIITIQN 68
LYE S+ KI++T+QN
Sbjct: 677 LYERRISIGKIVLTLQN 693
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,642,792
Number of Sequences: 5004
Number of extensions: 56400
Number of successful extensions: 139
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 133
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 137
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 283719918
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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