Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner10o13f
(593 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g18040.1 68417.m02685 eukaryotic translation initiation facto... 132 2e-31
At1g29550.1 68414.m03614 eukaryotic translation initiation facto... 119 2e-27
At1g29590.1 68414.m03618 eukaryotic translation initiation facto... 118 4e-27
At5g35620.2 68418.m04252 eukaryotic translation initiation facto... 117 5e-27
At5g35620.1 68418.m04251 eukaryotic translation initiation facto... 117 5e-27
At5g18110.1 68418.m02126 novel cap-binding protein (nCBP) identi... 117 7e-27
At5g67370.1 68418.m08495 expressed protein similar to unknown pr... 27 9.4
>At4g18040.1 68417.m02685 eukaryotic translation initiation factor
4E 1 / eIF-4E1 / mRNA cap-binding protein 1 (EIF4E1)
identical to SP|O23252 Eukaryotic translation initiation
factor 4E (eIF-4E) (eIF4E) (mRNA cap-binding protein)
(eIF-4F 25 kDa subunit) (eIF-4F P26 subunit)
{Arabidopsis thaliana}
Length = 235
Score = 132 bits (318), Expect = 2e-31
Identities = 62/142 (43%), Positives = 90/142 (63%), Gaps = 4/142 (2%)
Frame = +1
Query: 175 HPLQNQWSLWFYDN----DRNKTWEENLIELTTFDTVEDFWRLYHHIKLPSELRQGHDYA 342
HPL++ W+ WF DN + +W +L + TF TVE+FW LY+++K PS+L G D+
Sbjct: 60 HPLEHSWTFWF-DNPAVKSKQTSWGSSLRPVFTFSTVEEFWSLYNNMKHPSKLAHGADFY 118
Query: 343 VFKQGIRPMWEDDANKMGGRWLISLEKKQRFTDLDRFWLDVVLLLIGENFENSDEICGAV 522
FK I P WED GG+W ++ K++ D+ WL +L LIGE F++ DEICGAV
Sbjct: 119 CFKHIIEPKWEDPICANGGKWTMTFPKEKS----DKSWLYTLLALIGEQFDHGDEICGAV 174
Query: 523 VNVRPKVDKIAIWTADAMKQHA 588
VN+R K ++I+IWT +A + A
Sbjct: 175 VNIRGKQERISIWTKNASNEAA 196
>At1g29550.1 68414.m03614 eukaryotic translation initiation factor
4E, putative / eIF-4E, putative / eIF4E, putative / mRNA
cap-binding protein, putative similar to SP|O23252
Eukaryotic translation initiation factor 4E (eIF-4E)
(eIF4E) (mRNA cap-binding protein) (eIF-4F 25 kDa
subunit) (eIF-4F P26 subunit) {Arabidopsis thaliana};
contains Pfam profile PF01652: Eukaryotic initiation
factor 4E
Length = 240
Score = 119 bits (286), Expect = 2e-27
Identities = 58/142 (40%), Positives = 82/142 (57%), Gaps = 4/142 (2%)
Frame = +1
Query: 175 HPLQNQWSLWFYDNDRNKT----WEENLIELTTFDTVEDFWRLYHHIKLPSELRQGHDYA 342
H QN W+ WF DN +K+ W +L L TF T+E+FW LY++I P++ G D
Sbjct: 65 HCFQNSWTFWF-DNPSSKSNQVIWGSSLRSLYTFGTIEEFWSLYNNIHPPTKWVSGADLY 123
Query: 343 VFKQGIRPMWEDDANKMGGRWLISLEKKQRFTDLDRFWLDVVLLLIGENFENSDEICGAV 522
FK I P WED GG+W + K L+ WL+ +L L+GE F+ DEICGAV
Sbjct: 124 CFKDKIEPKWEDPICANGGKWSMMFPK----ATLECNWLNTLLALVGEQFDQGDEICGAV 179
Query: 523 VNVRPKVDKIAIWTADAMKQHA 588
+N R + D+I++WT +A + A
Sbjct: 180 LNFRARGDRISLWTKNAANEEA 201
>At1g29590.1 68414.m03618 eukaryotic translation initiation factor
4E, putative / eIF-4E, putative / eIF4E, putative / mRNA
cap-binding protein, putative similar to SP|O23252
Eukaryotic translation initiation factor 4E (eIF-4E)
(eIF4E) (mRNA cap-binding protein) (eIF-4F 25 kDa
subunit) (eIF-4F P26 subunit) {Arabidopsis thaliana};
contains Pfam profile PF01652: Eukaryotic initiation
factor 4E
Length = 285
Score = 118 bits (283), Expect = 4e-27
Identities = 57/142 (40%), Positives = 81/142 (57%), Gaps = 4/142 (2%)
Frame = +1
Query: 175 HPLQNQWSLWFYDNDRNKT----WEENLIELTTFDTVEDFWRLYHHIKLPSELRQGHDYA 342
H QN W+ WF DN +K+ W +L L TF T+E+FW LY++I P++ G D
Sbjct: 110 HCFQNSWTFWF-DNPSSKSNQVIWGSSLRSLYTFATIEEFWSLYNNIHPPTKWVSGSDLY 168
Query: 343 VFKQGIRPMWEDDANKMGGRWLISLEKKQRFTDLDRFWLDVVLLLIGENFENSDEICGAV 522
FK I P WED GG+W + + L+ WL+ +L L+GE F+ DEICGAV
Sbjct: 169 CFKDKIEPKWEDPICANGGKWTMFFPR----ATLESNWLNTLLALVGEQFDQGDEICGAV 224
Query: 523 VNVRPKVDKIAIWTADAMKQHA 588
+N R + D+I++WT A + A
Sbjct: 225 LNFRTRGDRISLWTKKAANEEA 246
>At5g35620.2 68418.m04252 eukaryotic translation initiation factor
4E 2 / eIF-4E2 / mRNA cap-binding protein 2 (EIF4E2)
identical to SP|O04663 Eukaryotic translation initiation
factor 4E (eIF-4E) (eIF4E) (mRNA cap-binding protein)
(eIF-(iso)4F 25 kDa subunit) (eIF-(ISO)4F P28 subunit)
(eIF4Eiso protein) {Arabidopsis thaliana}
Length = 167
Score = 117 bits (282), Expect = 5e-27
Identities = 58/136 (42%), Positives = 83/136 (61%), Gaps = 6/136 (4%)
Frame = +1
Query: 175 HPLQNQWSLWFYDNDRNK--TWEENLIELTTFDTVEDFWRLYHHIKLPSELRQGHDYAVF 348
H L+ +WS WF DN K W +L + TFDTVEDFW L+ I S+L + +F
Sbjct: 26 HKLERKWSFWF-DNQSKKGAAWGASLRKAYTFDTVEDFWGLHETIFQTSKLTANAEIHLF 84
Query: 349 KQGIRPMWEDDANKMGGR--WLISLEKKQRFTDLDRFWLDVVLLLIGENFENSDEICGAV 522
K G+ P WED GG+ W+++ +K+ LD+ WL+ ++ LIGE F+ +DEICG V
Sbjct: 85 KAGVEPKWEDPECANGGKWTWVVTANRKEA---LDKGWLETLMALIGEQFDEADEICGVV 141
Query: 523 VNVRP--KVDKIAIWT 564
+VRP K DK+++WT
Sbjct: 142 ASVRPQSKQDKLSLWT 157
>At5g35620.1 68418.m04251 eukaryotic translation initiation factor
4E 2 / eIF-4E2 / mRNA cap-binding protein 2 (EIF4E2)
identical to SP|O04663 Eukaryotic translation initiation
factor 4E (eIF-4E) (eIF4E) (mRNA cap-binding protein)
(eIF-(iso)4F 25 kDa subunit) (eIF-(ISO)4F P28 subunit)
(eIF4Eiso protein) {Arabidopsis thaliana}
Length = 198
Score = 117 bits (282), Expect = 5e-27
Identities = 58/136 (42%), Positives = 83/136 (61%), Gaps = 6/136 (4%)
Frame = +1
Query: 175 HPLQNQWSLWFYDNDRNK--TWEENLIELTTFDTVEDFWRLYHHIKLPSELRQGHDYAVF 348
H L+ +WS WF DN K W +L + TFDTVEDFW L+ I S+L + +F
Sbjct: 26 HKLERKWSFWF-DNQSKKGAAWGASLRKAYTFDTVEDFWGLHETIFQTSKLTANAEIHLF 84
Query: 349 KQGIRPMWEDDANKMGGR--WLISLEKKQRFTDLDRFWLDVVLLLIGENFENSDEICGAV 522
K G+ P WED GG+ W+++ +K+ LD+ WL+ ++ LIGE F+ +DEICG V
Sbjct: 85 KAGVEPKWEDPECANGGKWTWVVTANRKEA---LDKGWLETLMALIGEQFDEADEICGVV 141
Query: 523 VNVRP--KVDKIAIWT 564
+VRP K DK+++WT
Sbjct: 142 ASVRPQSKQDKLSLWT 157
>At5g18110.1 68418.m02126 novel cap-binding protein (nCBP) identical
to novel cap-binding protein nCBP [Arabidopsis thaliana]
GI:3108209; contains Pfam profile PF01652: Eukaryotic
initiation factor 4E
Length = 221
Score = 117 bits (281), Expect = 7e-27
Identities = 56/141 (39%), Positives = 84/141 (59%), Gaps = 3/141 (2%)
Frame = +1
Query: 175 HPLQNQWSLWFYDND---RNKTWEENLIELTTFDTVEDFWRLYHHIKLPSELRQGHDYAV 345
HPL+ ++S+W+ RN+++E+N+ ++ F TVE FW Y H+ S L D
Sbjct: 43 HPLRYKFSIWYTRRTPGVRNQSYEDNIKKMVEFSTVEGFWACYCHLARSSLLPSPTDLHF 102
Query: 346 FKQGIRPMWEDDANKMGGRWLISLEKKQRFTDLDRFWLDVVLLLIGENFENSDEICGAVV 525
FK GIRP+WED AN GG+W+I K RFW D++L L+G+ +++D ICGAV+
Sbjct: 103 FKDGIRPLWEDGANCNGGKWIIRFSK----VVSARFWEDLLLALVGDQLDDADNICGAVL 158
Query: 526 NVRPKVDKIAIWTADAMKQHA 588
+VR D I++W +A A
Sbjct: 159 SVRFNEDIISVWNRNASDHQA 179
>At5g67370.1 68418.m08495 expressed protein similar to unknown
protein (gb|AAC18972.1)
Length = 327
Score = 27.1 bits (57), Expect = 9.4
Identities = 13/49 (26%), Positives = 26/49 (53%), Gaps = 5/49 (10%)
Frame = +1
Query: 283 WRLYHHIKLPSELRQGHDYAVFKQGIRPMW-----EDDANKMGGRWLIS 414
W I++PS+ R ++Y+ K+G+ W + ++GG WL++
Sbjct: 78 WDPSSEIEVPSDQRPVNEYSSLKEGMLYSWGELGPSEFFIRLGGLWLVT 126
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,957,975
Number of Sequences: 28952
Number of extensions: 262493
Number of successful extensions: 665
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 635
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 652
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1180950720
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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