Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner10o10f
(643 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g41670.2 68418.m05063 6-phosphogluconate dehydrogenase family... 167 5e-42
At5g41670.1 68418.m05062 6-phosphogluconate dehydrogenase family... 167 5e-42
At1g64190.1 68414.m07272 6-phosphogluconate dehydrogenase family... 163 9e-41
At3g02360.2 68416.m00220 6-phosphogluconate dehydrogenase family... 157 6e-39
At3g02360.1 68416.m00219 6-phosphogluconate dehydrogenase family... 157 6e-39
At4g29120.1 68417.m04168 6-phosphogluconate dehydrogenase NAD-bi... 41 6e-04
At3g25530.1 68416.m03174 6-phosphogluconate dehydrogenase NAD-bi... 38 0.006
At2g03270.1 68415.m00280 DNA-binding protein, putative similar t... 31 0.86
At3g06350.1 68416.m00733 dehydroquinate dehydratase, putative / ... 29 2.6
At1g71180.1 68414.m08213 6-phosphogluconate dehydrogenase NAD-bi... 28 4.6
At1g44820.1 68414.m05134 aminoacylase, putative / N-acyl-L-amino... 28 4.6
At5g47560.1 68418.m05871 sodium/dicarboxylate cotransporter, put... 28 6.1
At1g56190.1 68414.m06458 phosphoglycerate kinase, putative simil... 28 6.1
At1g17650.1 68414.m02185 6-phosphogluconate dehydrogenase NAD-bi... 28 6.1
At5g09340.1 68418.m01082 ubiquitin, putative similar to ubiquiti... 27 8.0
At4g36430.1 68417.m05175 peroxidase, putative identical to perox... 27 8.0
At1g71170.1 68414.m08212 6-phosphogluconate dehydrogenase NAD-bi... 27 8.0
At1g54380.1 68414.m06200 spliceosome protein-related contains Pf... 27 8.0
>At5g41670.2 68418.m05063 6-phosphogluconate dehydrogenase family
protein contains Pfam profiles: PF00393
6-phosphogluconate dehydrogenase C-terminal domain,
PF03446 NAD binding domain of 6-phosphogluconate
Length = 487
Score = 167 bits (406), Expect = 5e-42
Identities = 84/172 (48%), Positives = 112/172 (65%), Gaps = 3/172 (1%)
Frame = +3
Query: 135 IALIGLAVMGQNLILNMNDKGYVVCAFNRTVSKVEEFL---KNEAKGTKVIGATSLDDMV 305
I L GLAVMGQNL LN+ DKG+ + +NRT SKV+E L NE K V G S D V
Sbjct: 9 IGLAGLAVMGQNLALNIADKGFPISVYNRTTSKVDETLDRASNEGK-LPVAGQYSPRDFV 67
Query: 306 SKLKRPRKIVLLVKAGFAVDEFVKKLIPLLSKGDIIIDGGNSQYLDTQKWCKELSGTGIL 485
++RPR +++LVKAG VD+ + L + GD IIDGGN Y +T++ E G+L
Sbjct: 68 LSIQRPRSVIILVKAGAPVDQTISALSEYMEPGDCIIDGGNEWYQNTERRIVEAEKKGLL 127
Query: 486 YVGMGVSGGEDGARYGPSLMPGGHPAAWPHIKEIFQAICAKAXDEPCCDWVG 641
Y+GMGVSGGE+GAR GPSLMPGG A+ ++K+I + + A+ D PC ++G
Sbjct: 128 YLGMGVSGGEEGARNGPSLMPGGSFTAYNNVKDILEKVAAQVEDGPCVTYIG 179
>At5g41670.1 68418.m05062 6-phosphogluconate dehydrogenase family
protein contains Pfam profiles: PF00393
6-phosphogluconate dehydrogenase C-terminal domain,
PF03446 NAD binding domain of 6-phosphogluconate
Length = 487
Score = 167 bits (406), Expect = 5e-42
Identities = 84/172 (48%), Positives = 112/172 (65%), Gaps = 3/172 (1%)
Frame = +3
Query: 135 IALIGLAVMGQNLILNMNDKGYVVCAFNRTVSKVEEFL---KNEAKGTKVIGATSLDDMV 305
I L GLAVMGQNL LN+ DKG+ + +NRT SKV+E L NE K V G S D V
Sbjct: 9 IGLAGLAVMGQNLALNIADKGFPISVYNRTTSKVDETLDRASNEGK-LPVAGQYSPRDFV 67
Query: 306 SKLKRPRKIVLLVKAGFAVDEFVKKLIPLLSKGDIIIDGGNSQYLDTQKWCKELSGTGIL 485
++RPR +++LVKAG VD+ + L + GD IIDGGN Y +T++ E G+L
Sbjct: 68 LSIQRPRSVIILVKAGAPVDQTISALSEYMEPGDCIIDGGNEWYQNTERRIVEAEKKGLL 127
Query: 486 YVGMGVSGGEDGARYGPSLMPGGHPAAWPHIKEIFQAICAKAXDEPCCDWVG 641
Y+GMGVSGGE+GAR GPSLMPGG A+ ++K+I + + A+ D PC ++G
Sbjct: 128 YLGMGVSGGEEGARNGPSLMPGGSFTAYNNVKDILEKVAAQVEDGPCVTYIG 179
>At1g64190.1 68414.m07272 6-phosphogluconate dehydrogenase family
protein contains Pfam profiles: PF00393
6-phosphogluconate dehydrogenase C-terminal domain,
PF03446 NAD binding domain of 6-phosphogluconate
Length = 487
Score = 163 bits (396), Expect = 9e-41
Identities = 82/171 (47%), Positives = 111/171 (64%), Gaps = 2/171 (1%)
Frame = +3
Query: 135 IALIGLAVMGQNLILNMNDKGYVVCAFNRTVSKVEEFLKNEA-KGT-KVIGATSLDDMVS 308
I L GLAVMGQNL LN+ +KG+ + +NRT SKV+E L A +G V G S D V
Sbjct: 9 IGLAGLAVMGQNLALNIAEKGFPISVYNRTTSKVDETLDRAAVEGNLPVSGQYSPRDFVL 68
Query: 309 KLKRPRKIVLLVKAGFAVDEFVKKLIPLLSKGDIIIDGGNSQYLDTQKWCKELSGTGILY 488
++RPR +++LVKAG VD+ + + GD IIDGGN Y +T++ E G+LY
Sbjct: 69 SIQRPRSLIILVKAGAPVDQTIDAFSEYMEPGDCIIDGGNEWYQNTERRISEAEQKGLLY 128
Query: 489 VGMGVSGGEDGARYGPSLMPGGHPAAWPHIKEIFQAICAKAXDEPCCDWVG 641
+GMGVSGGE+GAR GPSLMPGG A+ +IK+I + + A+ D PC ++G
Sbjct: 129 LGMGVSGGEEGARNGPSLMPGGSFQAYDNIKDILEKVAAQVEDGPCVTYIG 179
>At3g02360.2 68416.m00220 6-phosphogluconate dehydrogenase family
protein contains Pfam profiles: PF00393
6-phosphogluconate dehydrogenase C-terminal domain,
PF03446 NAD binding domain of 6-phosphogluconate
;similar to 6-phosphogluconate dehydrogenase GB:BAA22812
GI:2529229 [Glycine max]
Length = 486
Score = 157 bits (381), Expect = 6e-39
Identities = 78/172 (45%), Positives = 112/172 (65%), Gaps = 3/172 (1%)
Frame = +3
Query: 135 IALIGLAVMGQNLILNMNDKGYVVCAFNRTVSKVEEFLKNEAK--GTKVIGATSLDDMVS 308
I L GLAVMGQNL LN+ +KG+ + +NRT SKV+E ++ K + G + V
Sbjct: 8 IGLAGLAVMGQNLALNIAEKGFPISVYNRTTSKVDETVERAKKEGNLPLYGFHDPESFVK 67
Query: 309 KLKRPRKIVLLVKAGFAVDEFVKKLIPLLSKGDIIIDGGNSQYLDTQKWCKELSGTGILY 488
+++PR I++LVKAG VD+ +K L L KGD I+DGGN Y +T++ K ++ G LY
Sbjct: 68 SIQKPRVIIMLVKAGSPVDQTIKTLSAYLEKGDCIVDGGNEWYENTERREKAVAENGFLY 127
Query: 489 VGMGVSGGEDGARYGPSLMPGGHPAAWPHIKEIFQAICAKAXDE-PCCDWVG 641
+GMGVSGGE+GAR GPS+MPGG A+ +I++I + A+ D PC ++G
Sbjct: 128 LGMGVSGGEEGARNGPSMMPGGSYEAYKNIEDIVLKVAAQVRDSGPCVTYIG 179
>At3g02360.1 68416.m00219 6-phosphogluconate dehydrogenase family
protein contains Pfam profiles: PF00393
6-phosphogluconate dehydrogenase C-terminal domain,
PF03446 NAD binding domain of 6-phosphogluconate
;similar to 6-phosphogluconate dehydrogenase GB:BAA22812
GI:2529229 [Glycine max]
Length = 486
Score = 157 bits (381), Expect = 6e-39
Identities = 78/172 (45%), Positives = 112/172 (65%), Gaps = 3/172 (1%)
Frame = +3
Query: 135 IALIGLAVMGQNLILNMNDKGYVVCAFNRTVSKVEEFLKNEAK--GTKVIGATSLDDMVS 308
I L GLAVMGQNL LN+ +KG+ + +NRT SKV+E ++ K + G + V
Sbjct: 8 IGLAGLAVMGQNLALNIAEKGFPISVYNRTTSKVDETVERAKKEGNLPLYGFHDPESFVK 67
Query: 309 KLKRPRKIVLLVKAGFAVDEFVKKLIPLLSKGDIIIDGGNSQYLDTQKWCKELSGTGILY 488
+++PR I++LVKAG VD+ +K L L KGD I+DGGN Y +T++ K ++ G LY
Sbjct: 68 SIQKPRVIIMLVKAGSPVDQTIKTLSAYLEKGDCIVDGGNEWYENTERREKAVAENGFLY 127
Query: 489 VGMGVSGGEDGARYGPSLMPGGHPAAWPHIKEIFQAICAKAXDE-PCCDWVG 641
+GMGVSGGE+GAR GPS+MPGG A+ +I++I + A+ D PC ++G
Sbjct: 128 LGMGVSGGEEGARNGPSMMPGGSYEAYKNIEDIVLKVAAQVRDSGPCVTYIG 179
>At4g29120.1 68417.m04168 6-phosphogluconate dehydrogenase
NAD-binding domain-containing protein similar to
SP|P23523 2-hydroxy-3-oxopropionate reductase (EC
1.1.1.60) (Tartronate semialdehyde reductase)
{Escherichia coli}; contains Pfam profile PF03446: NAD
binding domain of 6-phosphogluconate dehydrogenase
Length = 334
Score = 41.1 bits (92), Expect = 6e-04
Identities = 42/164 (25%), Positives = 70/164 (42%), Gaps = 6/164 (3%)
Frame = +3
Query: 117 PQNEADIALIGLAVMGQNLILNMNDKGYVVCAFNRTVSKVEEFL---KNEAKGTKVIGAT 287
P N I IG VMG+++ ++ GY V FNRT+SK + + N A +
Sbjct: 35 PSN-TKIGWIGTGVMGRSMCGHLIKAGYTVTVFNRTISKAQTLIDMGANVADSPNSVAEQ 93
Query: 288 S--LDDMVSKLKRPRKIVLLVKAGFAVDEFVKKLIPLLSKGDIIIDGGNSQYLDTQKWCK 461
S + +V R ++L K+G + L +G +++D S+ ++ K
Sbjct: 94 SDVVFTIVGYPSDVRHVLLDPKSG---------ALSGLRQGGVLVDMTTSEPSLAEEIAK 144
Query: 462 ELSGTGILYVGMGVSGGEDGARYGP-SLMPGGHPAAWPHIKEIF 590
S + VSGG+ GA+ G S+ GG + +F
Sbjct: 145 AASFKNCFSIDAPVSGGDLGAKNGKLSIFAGGDETTVKRLDPLF 188
>At3g25530.1 68416.m03174 6-phosphogluconate dehydrogenase
NAD-binding domain-containing protein low similarity to
SP|P23523 2-hydroxy-3-oxopropionate reductase (EC
1.1.1.60) (Tartronate semialdehyde reductase)
{Escherichia coli}; contains Pfam profile PF03446: NAD
binding domain of 6-phosphogluconate dehydrogenase;
supporting cDNA gi|15375067|gb|AY044183.1|
Length = 289
Score = 37.9 bits (84), Expect = 0.006
Identities = 14/43 (32%), Positives = 29/43 (67%)
Frame = +3
Query: 132 DIALIGLAVMGQNLILNMNDKGYVVCAFNRTVSKVEEFLKNEA 260
++ +GL +MG+ + +N+ G+ V +NRT+SK +E +++ A
Sbjct: 2 EVGFLGLGIMGKAMSMNLLKNGFKVTVWNRTLSKCDELVEHGA 44
>At2g03270.1 68415.m00280 DNA-binding protein, putative similar to
Swiss-Prot:Q60560 DNA-binding protein SMUBP-2
(Immunoglobulin MU binding protein 2) (SMUBP-2) (Insulin
II gene enhancer-binding protein)(RIPE3B-binding complex
3B2 P110 subunit) (RIP-1)[Mesocricetus auratus];
identical to putative helicase (atpc-2 gene) cDNA
NCBI_gi:11191230
Length = 639
Score = 30.7 bits (66), Expect = 0.86
Identities = 17/60 (28%), Positives = 32/60 (53%)
Frame = +3
Query: 210 AFNRTVSKVEEFLKNEAKGTKVIGATSLDDMVSKLKRPRKIVLLVKAGFAVDEFVKKLIP 389
A + +S + FL + GT T ++ ++ ++KR KI+ + AVD V++L+P
Sbjct: 197 AITKALSSKDVFLLHGPPGTGKT-TTVVEIVLQEVKRGSKILACAASNIAVDNIVERLVP 255
>At3g06350.1 68416.m00733 dehydroquinate dehydratase, putative /
shikimate dehydrogenase, putative similar to
dehydroquinate dehydratase/shikimate dehydrogenase
[Nicotiana tabacum][GI:535771], dehydroquinate
dehydratase/shikimate:NADP oxidoreductase [Lycopersicon
esculentum][GI:3169883]
Length = 603
Score = 29.1 bits (62), Expect = 2.6
Identities = 19/61 (31%), Positives = 26/61 (42%)
Frame = +3
Query: 117 PQNEADIALIGLAVMGQNLILNMNDKGYVVCAFNRTVSKVEEFLKNEAKGTKVIGATSLD 296
P + +IG G+ L +KG V NRT + E EA G K + T LD
Sbjct: 450 PLASKTVVVIGAGGAGKALAYGAKEKGAKVVIANRTYERALELA--EAIGGKALSLTDLD 507
Query: 297 D 299
+
Sbjct: 508 N 508
>At1g71180.1 68414.m08213 6-phosphogluconate dehydrogenase
NAD-binding domain-containing protein similar to
SP|P23523 2-hydroxy-3-oxopropionate reductase (EC
1.1.1.60) (Tartronate semialdehyde reductase)
{Escherichia coli}; contains Pfam profile PF03446: NAD
binding domain of 6-phosphogluconate dehydrogenase
Length = 318
Score = 28.3 bits (60), Expect = 4.6
Identities = 35/154 (22%), Positives = 67/154 (43%), Gaps = 5/154 (3%)
Frame = +3
Query: 108 KKMPQNEADIALIGLAVMGQNLILNMNDKGYVVCAFNRTVSKVEEFLKNEAKGTKVIGA- 284
K + ++ I IG+ +MG ++ ++ GY V + R + K ++ + KG ++ +
Sbjct: 28 KLIDPSKTRIGWIGIGIMGSAMVSHIIAAGYSVTVYARDLRKTKDL---QTKGARIANSP 84
Query: 285 ---TSLDDMVSKLKRPRKIVLLVKAGFAVDEFVKKLIPLLSKGDIIIDGGNSQYLDTQKW 455
+ D+V + V + G D+ V + L+ G + +D +S+ ++
Sbjct: 85 KELAEMSDVVFTIVGNFNDVRSLLLG---DDGV---LSGLTPGGVTVDMTSSKPGLAREI 138
Query: 456 CKELSGTGILYVGMGVSGGEDGARYGP-SLMPGG 554
E V VSGG+ GAR G + GG
Sbjct: 139 HAEARRRNCWAVDAPVSGGDAGAREGTLGIFAGG 172
>At1g44820.1 68414.m05134 aminoacylase, putative /
N-acyl-L-amino-acid amidohydrolase, putative similar to
aminoacylase-1 (N-acyl-L-amino-acid amidohydrolase
ACY-1)[Homo sapiens] SWISS-PROT:Q03154
Length = 438
Score = 28.3 bits (60), Expect = 4.6
Identities = 20/60 (33%), Positives = 30/60 (50%)
Frame = -1
Query: 355 NPAFTKRTIFLGLFSLDTMSSKEVAPITFVPLASFFRNSSTFDTVLLNAQTT*PLSFILS 176
+P IF +FSL + SS+E PIT F+ F+T N T P+SF+++
Sbjct: 4 SPLLWTLIIFSIIFSLQSSSSEEDTPIT------RFQQYLRFNTAHPNPNYTAPISFLIN 57
>At5g47560.1 68418.m05871 sodium/dicarboxylate cotransporter,
putative similar to SWISS-PROT:Q13183 renal
sodium/dicarboxylate cotransporter [Human]{Homo sapiens}
Length = 540
Score = 27.9 bits (59), Expect = 6.1
Identities = 16/37 (43%), Positives = 24/37 (64%), Gaps = 2/37 (5%)
Frame = +3
Query: 315 KRPRKIVLLVKAGFAVDEFVKK--LIPLLSKGDIIID 419
K P IVLL+ AGFA+ + V+ L +LSKG + ++
Sbjct: 387 KLPWNIVLLLGAGFAIADGVRTSGLAEVLSKGLVFLE 423
>At1g56190.1 68414.m06458 phosphoglycerate kinase, putative similar
to SP|P41758 Phosphoglycerate kinase, chloroplast
precursor (EC 2.7.2.3) {Chlamydomonas reinhardtii};
contains Pfam profile PF00162: phosphoglycerate kinase
Length = 478
Score = 27.9 bits (59), Expect = 6.1
Identities = 28/106 (26%), Positives = 45/106 (42%)
Frame = +3
Query: 108 KKMPQNEADIALIGLAVMGQNLILNMNDKGYVVCAFNRTVSKVEEFLKNEAKGTKVIGAT 287
K+ +NE D A LA + L +ND + + V +FLK G +
Sbjct: 197 KEEEKNEPDFAK-KLASLAD---LYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLL--QK 250
Query: 288 SLDDMVSKLKRPRKIVLLVKAGFAVDEFVKKLIPLLSKGDIIIDGG 425
LD +V + P++ + G V + + LL K DI++ GG
Sbjct: 251 ELDYLVGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGG 296
>At1g17650.1 68414.m02185 6-phosphogluconate dehydrogenase
NAD-binding domain-containing protein low similarity to
SP|P23523 2-hydroxy-3-oxopropionate reductase (EC
1.1.1.60) (Tartronate semialdehyde reductase)
{Escherichia coli}; contains Pfam profile PF03446: NAD
binding domain of 6-phosphogluconate dehydrogenase
Length = 343
Score = 27.9 bits (59), Expect = 6.1
Identities = 14/43 (32%), Positives = 22/43 (51%)
Frame = +3
Query: 135 IALIGLAVMGQNLILNMNDKGYVVCAFNRTVSKVEEFLKNEAK 263
I +G+ +MG + N+ G V +NRT SK + + AK
Sbjct: 54 IGFLGMGIMGSPMAQNLIKAGCDVTVWNRTKSKCDPLVGLGAK 96
>At5g09340.1 68418.m01082 ubiquitin, putative similar to ubiquitin
SP:P42740 from [Aglaothamnion neglectum]
Length = 79
Score = 27.5 bits (58), Expect = 8.0
Identities = 14/47 (29%), Positives = 23/47 (48%)
Frame = +3
Query: 267 TKVIGATSLDDMVSKLKRPRKIVLLVKAGFAVDEFVKKLIPLLSKGD 407
TK+ AT +++ L+ KIVL V G +D++ K + D
Sbjct: 28 TKIYDATGMENAYQYLEHRGKIVLEVHGGTTMDDYKIKACDIFHMSD 74
>At4g36430.1 68417.m05175 peroxidase, putative identical to
peroxidase [Arabidopsis thaliana]
gi|6822093|emb|CAB71009; identical to cDNA class III
peroxidase ATP31, GI:17530561
Length = 331
Score = 27.5 bits (58), Expect = 8.0
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +3
Query: 528 YGPSLMPGGHPAAWPHIKEIFQAICAKA 611
YG L PG + + P + EI +++ AKA
Sbjct: 26 YGGKLFPGYYAHSCPQVNEIVRSVVAKA 53
>At1g71170.1 68414.m08212 6-phosphogluconate dehydrogenase
NAD-binding domain-containing protein contains Pfam
profile: PF03446 NAD binding domain of
6-phosphogluconate
Length = 299
Score = 27.5 bits (58), Expect = 8.0
Identities = 36/153 (23%), Positives = 65/153 (42%), Gaps = 4/153 (2%)
Frame = +3
Query: 108 KKMPQNEADIALIGLAVMGQNLILNMNDKGYVVCAFNRTVSKVEEFLK---NEAKGTKVI 278
K + ++ I IG+ +MG ++ ++ GY V + R + K ++ A K +
Sbjct: 7 KLIEPSKTRIGWIGIGIMGSAMVSHILAAGYSVTVYARDLRKTKDLQTKGGRTANSPKEL 66
Query: 279 GATSLDDMVSKLKRPRKIVLLVKAGFAVDEFVKKLIPLLSKGDIIIDGGNSQYLDTQKWC 458
G + D+V + V + G D+ V + L G + +D +S+ ++
Sbjct: 67 G--EMSDVVFTIVGNSNDVRSLLLG---DDGV---LSGLKPGGVTVDMTSSKPGLAREIY 118
Query: 459 KELSGTGILYVGMGVSGGEDGARYGP-SLMPGG 554
E V VSGG+ GAR G ++ GG
Sbjct: 119 AEARRRDCWAVDAPVSGGDAGAREGKLTIFAGG 151
>At1g54380.1 68414.m06200 spliceosome protein-related contains Pfam
domain, PF04938: Survival motor neuron (SMN) interacting
protein 1 (SIP1)
Length = 515
Score = 27.5 bits (58), Expect = 8.0
Identities = 12/34 (35%), Positives = 21/34 (61%)
Frame = +3
Query: 222 TVSKVEEFLKNEAKGTKVIGATSLDDMVSKLKRP 323
T+ K+EE + N+ +++ T+L D+V KRP
Sbjct: 127 TLPKIEETMDNDVDKIELVDHTALVDVVHHPKRP 160
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,612,788
Number of Sequences: 28952
Number of extensions: 272146
Number of successful extensions: 737
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 717
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 732
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1324661040
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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