Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner10m18f
(640 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pomb... 148 6e-37
SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 31 0.19
SPAPB1A10.07c |||sphingolipid biosynthesis protein|Schizosacchar... 29 0.43
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 28 1.3
SPBC1347.04 |tim54||TIM22 inner membrane protein import complex ... 28 1.3
SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit Psm3|Sch... 27 2.3
SPAC3H8.05c |||conserved fungal protein|Schizosaccharomyces pomb... 26 4.0
SPCC320.03 |||transcription factor |Schizosaccharomyces pombe|ch... 25 7.0
SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyc... 25 7.0
SPBC1709.14 |||peptide N-glycanase |Schizosaccharomyces pombe|ch... 25 7.0
SPBC16A3.07c |nrm1||negative regulator of MBF|Schizosaccharomyce... 25 7.0
SPAC664.10 |klp2||kinesin-like protein Klp2|Schizosaccharomyces ... 25 7.0
SPCC24B10.20 |||short chain dehydrogenase |Schizosaccharomyces p... 25 9.2
SPBC146.12 |coq6||monooxygenase Coq6|Schizosaccharomyces pombe|c... 25 9.2
SPAC19A8.10 |rfp1|mug140|ubiquitin-protein ligase E3 Rfp1|Schizo... 25 9.2
>SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 330
Score = 148 bits (359), Expect = 6e-37
Identities = 69/170 (40%), Positives = 105/170 (61%)
Frame = +1
Query: 130 KVTIVGVGQVGMAAAFSMLTQNVTNNIALVDMMADKLKGEMMDLQHGSAFMRNAKIQSST 309
K+ IVG G VG AF++L + I ++D+ K +GE MDL H + ++
Sbjct: 22 KIVIVGAGNVGSTTAFTLLLSGLAAEIVIIDLNKKKAEGEAMDLNHAAPLSHETRVYLG- 80
Query: 310 DYSITAGSKICVVTAGVRQREGESRLDLVQRNTDVLKQIIPQLIKYSPDTILVIASNPVD 489
DY + V+TAG Q+ GE+R+DL++ N + K+I+ ++ KY+ D IL++A+NPVD
Sbjct: 81 DYKDCKDATAVVITAGKNQKPGETRMDLLKANISIFKEILREVTKYTKDAILLVATNPVD 140
Query: 490 ILTYVTWKISGLPKHRVIGSGTNLDSARFRYLLSDRLGIATTSCHGYIIG 639
+LTY T K++G P RVIGSGT +D+ARF+YL+ G+ S + IIG
Sbjct: 141 VLTYATLKLTGFPAERVIGSGTIIDTARFQYLIGKLYGLDPQSVNADIIG 190
>SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 30.7 bits (66), Expect = 0.19
Identities = 23/74 (31%), Positives = 38/74 (51%), Gaps = 1/74 (1%)
Frame = +1
Query: 103 HEKVDETWSKVTIVGVGQVGMAAAFSMLTQNVTNNIALVDMMADKLKGEMMDLQHGSA-F 279
H E+ S V +VG G VG+AA + + IA +D+ ADK E+ + G+ F
Sbjct: 187 HSAKVESGSTVAVVGCGCVGLAAMQGAVAAGASRIIA-IDINADK---EVYAKKFGATDF 242
Query: 280 MRNAKIQSSTDYSI 321
+ ++K++ Y I
Sbjct: 243 IDSSKVKDLVQYVI 256
>SPAPB1A10.07c |||sphingolipid biosynthesis
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 441
Score = 29.5 bits (63), Expect = 0.43
Identities = 22/107 (20%), Positives = 45/107 (42%), Gaps = 2/107 (1%)
Frame = -2
Query: 531 LGQPANLPRNIRQNIHGVTGDHQYCVRTVLYQLRDYLFKNIGVSLHEIETRFTFALSNTS 352
+G ++P + ++ GV G + T + L + + N+G + F +L +
Sbjct: 1 MGAVLSIPLALASSLSGVVGIASSFLVTSVLSLTNSIQSNVGAVISYAVLYFVNSLLSWC 60
Query: 351 SNHADLRASCYRIIRTRLDLGVPHE--C*SVLQVHHLSFQFVSHHVY 217
+ + ++ L ++ C SV+ VH LSF V H++
Sbjct: 61 MLSSWFNSKLSKLSAGYLQFDCQNDGKCYSVIAVHRLSFTLVMFHLF 107
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 27.9 bits (59), Expect = 1.3
Identities = 13/34 (38%), Positives = 21/34 (61%)
Frame = +2
Query: 533 TASSGPALTWTRHGSVTCCRTGSVSLPRPATATS 634
T++S P +T + + + TGS S P P+T+TS
Sbjct: 81 TSASTPIITESTSSTSSASTTGSSSSPLPSTSTS 114
>SPBC1347.04 |tim54||TIM22 inner membrane protein import complex
subunit Tim54|Schizosaccharomyces pombe|chr 2|||Manual
Length = 347
Score = 27.9 bits (59), Expect = 1.3
Identities = 12/48 (25%), Positives = 21/48 (43%)
Frame = -2
Query: 522 PANLPRNIRQNIHGVTGDHQYCVRTVLYQLRDYLFKNIGVSLHEIETR 379
P LPR ++ +HG GD Y R + +F + +E++
Sbjct: 56 PLELPRKLKVYLHGPPGDGIYVAREEFEEYIRPIFNAAAIEFETVESK 103
>SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit
Psm3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1194
Score = 27.1 bits (57), Expect = 2.3
Identities = 11/38 (28%), Positives = 21/38 (55%)
Frame = +1
Query: 232 DKLKGEMMDLQHGSAFMRNAKIQSSTDYSITAGSKICV 345
+++K E+ +L H +R K Q+ DY+ SK+ +
Sbjct: 268 ERIKAEITELNHSLELLRVEKQQNDEDYTNIMKSKVAL 305
>SPAC3H8.05c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1073
Score = 26.2 bits (55), Expect = 4.0
Identities = 21/76 (27%), Positives = 33/76 (43%), Gaps = 1/76 (1%)
Frame = +1
Query: 415 LKQIIPQLIKYSPDTILVIASNPVDILTYVTWKISGLPKHRVIGSGTNLDSARFRYLLSD 594
L I L K + ++I+SN + YVT + G+P +V + + D SD
Sbjct: 611 LPHSISYLRKSNRAVYVLISSNSTLLTVYVTLTLEGVPDFKVYSNPISTDLPLTLQSPSD 670
Query: 595 RLG-IATTSCHGYIIG 639
I S + YI+G
Sbjct: 671 EFSTIYAWSDYLYIVG 686
>SPCC320.03 |||transcription factor |Schizosaccharomyces pombe|chr
3|||Manual
Length = 867
Score = 25.4 bits (53), Expect = 7.0
Identities = 9/45 (20%), Positives = 23/45 (51%)
Frame = +1
Query: 409 DVLKQIIPQLIKYSPDTILVIASNPVDILTYVTWKISGLPKHRVI 543
D + ++P + + V +++P + +Y T SG+ +H ++
Sbjct: 333 DRVSNVLPSITSFDSSVTTVPSNSPATLNSYTTSVPSGMSRHPML 377
>SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1016
Score = 25.4 bits (53), Expect = 7.0
Identities = 12/40 (30%), Positives = 20/40 (50%)
Frame = -3
Query: 461 IVSGLYFISCGIICLRTSVFLCTRSRRDSPSRCRTPAVTT 342
I+ G+ ++SC C S ++C+ SP+ T TT
Sbjct: 901 IIDGVDYLSCNGACYNPSQYVCSDGSL-SPNTVTTTKATT 939
>SPBC1709.14 |||peptide N-glycanase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 333
Score = 25.4 bits (53), Expect = 7.0
Identities = 8/25 (32%), Positives = 16/25 (64%)
Frame = +3
Query: 21 LQALRDQSHGTLTEPQDGVPEEAVP 95
+ ++RD SH + P++G+P + P
Sbjct: 235 IDSVRDVSHRYIRHPENGLPRDRCP 259
>SPBC16A3.07c |nrm1||negative regulator of MBF|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 342
Score = 25.4 bits (53), Expect = 7.0
Identities = 20/72 (27%), Positives = 35/72 (48%), Gaps = 4/72 (5%)
Frame = -2
Query: 363 SNTSSNHADLRASCYRIIRTRLDLGV----PHEC*SVLQVHHLSFQFVSHHVY*SDVIRN 196
+NT++NH D C + +R RL+L + ++ S LQ + + H+ S+ + +
Sbjct: 120 TNTNNNHLDDIQCCAKNLRLRLELAMYKVQVNQTFSPLQDLPIVAKTKLHNCPNSEPVTS 179
Query: 195 ILRQHRESCGHP 160
I Q S G P
Sbjct: 180 IWNQRSLSSGKP 191
>SPAC664.10 |klp2||kinesin-like protein Klp2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 817
Score = 25.4 bits (53), Expect = 7.0
Identities = 15/40 (37%), Positives = 20/40 (50%), Gaps = 1/40 (2%)
Frame = +1
Query: 457 TILVIASNPVDILTYVTWKISGLPKHR-VIGSGTNLDSAR 573
TI I S P+D VTW + K+R V + N S+R
Sbjct: 644 TITNITSEPLDTPEQVTWLLDQASKNRSVAATNANEHSSR 683
Score = 25.0 bits (52), Expect = 9.2
Identities = 14/38 (36%), Positives = 20/38 (52%)
Frame = +2
Query: 527 PSTASSGPALTWTRHGSVTCCRTGSVSLPRPATATSSA 640
P TASS P + + H + + S RPA+A SS+
Sbjct: 91 PFTASSNPRMPKSAHPISSRSVSASSHFGRPASAVSSS 128
>SPCC24B10.20 |||short chain dehydrogenase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 254
Score = 25.0 bits (52), Expect = 9.2
Identities = 18/58 (31%), Positives = 26/58 (44%), Gaps = 2/58 (3%)
Frame = +1
Query: 79 LKKLFQPVHEKVDETW-SKVTIVGVGQVGMAAAF-SMLTQNVTNNIALVDMMADKLKG 246
+ K FQPV + DE W S +G + + AF +L + NI MA + G
Sbjct: 93 ISKSFQPVLKTSDELWMSHYQTNVLGPIHVYQAFYHLLKEGKLKNIVFTSSMAACMGG 150
>SPBC146.12 |coq6||monooxygenase Coq6|Schizosaccharomyces pombe|chr
2|||Manual
Length = 466
Score = 25.0 bits (52), Expect = 9.2
Identities = 16/48 (33%), Positives = 27/48 (56%), Gaps = 2/48 (4%)
Frame = +1
Query: 106 EKVDETWSKVTIVGVGQVGMAAAFSMLTQNVTNN--IALVDMMADKLK 243
+K+ + V IVG G VG+A A + + VT + + L+D+ D +K
Sbjct: 18 QKILQRQFDVVIVGSGPVGLALAAGLQSNPVTQSLKVGLLDIQ-DTMK 64
>SPAC19A8.10 |rfp1|mug140|ubiquitin-protein ligase E3
Rfp1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 254
Score = 25.0 bits (52), Expect = 9.2
Identities = 12/40 (30%), Positives = 18/40 (45%)
Frame = -2
Query: 609 SDTEPVRQQVTEPCRVQVSAGPDDAVLGQPANLPRNIRQN 490
S + PV ++ + + A PDD+ P PR R N
Sbjct: 20 SPSPPVETSISSTNIIDLDAIPDDSFPSSPVLSPRRRRMN 59
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,668,574
Number of Sequences: 5004
Number of extensions: 55602
Number of successful extensions: 182
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 170
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 181
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 285732116
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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