Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner10m18f
(640 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g17260.1 68417.m02595 L-lactate dehydrogenase, putative stron... 220 5e-58
At1g53240.1 68414.m06033 malate dehydrogenase [NAD], mitochondri... 51 7e-07
At3g15020.1 68416.m01900 malate dehydrogenase [NAD], mitochondri... 48 5e-06
At2g09900.1 68415.m01028 hypothetical protein 32 0.37
At5g13230.1 68418.m01520 pentatricopeptide (PPR) repeat-containi... 31 0.49
At4g04720.1 68417.m00693 calcium-dependent protein kinase, putat... 29 2.6
At2g41360.1 68415.m05105 F-box family protein similar to SKP1 in... 29 2.6
At5g19740.1 68418.m02347 peptidase M28 family protein ileal pept... 28 4.5
At2g36720.1 68415.m04505 PHD finger transcription factor, putative 28 6.0
At1g51460.1 68414.m05792 ABC transporter family protein similar ... 28 6.0
At5g53460.1 68418.m06644 glutamate synthase [NADH], chloroplast,... 27 7.9
At4g07670.1 68417.m01203 protease-associated (PA) domain-contain... 27 7.9
At3g47030.1 68416.m05107 F-box family protein contains F-box dom... 27 7.9
At3g07400.1 68416.m00882 lipase class 3 family protein contains ... 27 7.9
>At4g17260.1 68417.m02595 L-lactate dehydrogenase, putative strong
similarity to L-lactate dehydrogenase from Lycopersicon
esculentum (GI:1620970, GI:1620972), Hordeum vulgare
(SP|P22988, SP|P22989); contains InterPro entry
IPR001236: Lactate/malate dehydrogenase
Length = 353
Score = 220 bits (538), Expect = 5e-58
Identities = 103/187 (55%), Positives = 137/187 (73%), Gaps = 4/187 (2%)
Frame = +1
Query: 91 FQPVHEKVDETWS----KVTIVGVGQVGMAAAFSMLTQNVTNNIALVDMMADKLKGEMMD 258
F+P+H S KV++VGVG VGMA A ++LTQ++ + IALVD DKL+GEM+D
Sbjct: 25 FKPIHNSDPSLPSNRRTKVSVVGVGNVGMAIAQTILTQDLADEIALVDAKPDKLRGEMLD 84
Query: 259 LQHGSAFMRNAKIQSSTDYSITAGSKICVVTAGVRQREGESRLDLVQRNTDVLKQIIPQL 438
LQH +AF+ KI +S DY +TAGS +C+VTAG RQ GESRL+L+QRN + + IIP L
Sbjct: 85 LQHAAAFLPRTKITASVDYEVTAGSDLCIVTAGARQNPGESRLNLLQRNVALFRHIIPPL 144
Query: 439 IKYSPDTILVIASNPVDILTYVTWKISGLPKHRVIGSGTNLDSARFRYLLSDRLGIATTS 618
K SPD+IL+I SNPVD+LTYV WK+SG P +RV+GSGTNLDS+RFR+L++D L +
Sbjct: 145 AKASPDSILIIVSNPVDVLTYVAWKLSGFPVNRVLGSGTNLDSSRFRFLIADHLDVNAQD 204
Query: 619 CHGYIIG 639
+I+G
Sbjct: 205 VQAFIVG 211
>At1g53240.1 68414.m06033 malate dehydrogenase [NAD], mitochondrial
identical to mitochondrial NAD-dependent malate
dehydrogenase GI:3929649 SP|Q9ZP06 from [Arabidopsis
thaliana]; contains InterPro entry IPR001236:
Lactate/malate dehydrogenase
Length = 341
Score = 50.8 bits (116), Expect = 7e-07
Identities = 39/175 (22%), Positives = 77/175 (44%), Gaps = 5/175 (2%)
Frame = +1
Query: 130 KVTIVGV-GQVGMAAAFSMLTQNVTNNIALVDMMADKLKGEMMDLQHGSAFMRNAKIQSS 306
KV I+G G +G A M + ++++L D+ G D+ H +
Sbjct: 31 KVAILGAAGGIGQPLALLMKLNPLVSSLSLYDIA--NTPGVAADVGHINTRSEVVGYMGD 88
Query: 307 TDYS-ITAGSKICVVTAGVRQREGESRLDLVQRNTDVLKQIIPQLIKYSPDTILVIASNP 483
+ + G+ + ++ AGV ++ G +R DL N ++K + + KY P ++ + SNP
Sbjct: 89 DNLAKALEGADLVIIPAGVPRKPGMTRDDLFNINAGIVKNLCTAIAKYCPHALINMISNP 148
Query: 484 VDILTYVT---WKISGLPKHRVIGSGTNLDSARFRYLLSDRLGIATTSCHGYIIG 639
V+ + +K +G+ + + T LD R R + + + + +IG
Sbjct: 149 VNSTVPIAAEIFKKAGMYDEKKLFGVTTLDVVRARTFYAGKANVPVAEVNVPVIG 203
>At3g15020.1 68416.m01900 malate dehydrogenase [NAD], mitochondrial,
putative similar to mitochondrial NAD-dependent malate
dehydrogenase GB:CAA10320 SP|Q9ZP06 [Arabidopsis
thaliana]; contains InterPro entry IPR001236:
Lactate/malate dehydrogenase
Length = 341
Score = 48.0 bits (109), Expect = 5e-06
Identities = 38/175 (21%), Positives = 77/175 (44%), Gaps = 5/175 (2%)
Frame = +1
Query: 130 KVTIVGV-GQVGMAAAFSMLTQNVTNNIALVDMMADKLKGEMMDLQHGSAFMRNAKIQSS 306
KV I+G G +G + M + ++++L D+ G D+ H + + +
Sbjct: 31 KVVILGAAGGIGQPLSLLMKLNPLVSSLSLYDIA--NTPGVAADVGHINTRSQVSGYMGD 88
Query: 307 TDYS-ITAGSKICVVTAGVRQREGESRLDLVQRNTDVLKQIIPQLIKYSPDTILVIASNP 483
D G+ + ++ AGV ++ G +R DL N ++K + + KY P ++ + SNP
Sbjct: 89 DDLGKALEGADLVIIPAGVPRKPGMTRDDLFNINAGIVKNLSIAIAKYCPQALVNMISNP 148
Query: 484 VDILTYVT---WKISGLPKHRVIGSGTNLDSARFRYLLSDRLGIATTSCHGYIIG 639
V+ + +K +G + + T LD R R + + + + ++G
Sbjct: 149 VNSTVPIAAEIFKKAGTYDEKKLFGVTTLDVVRARTFYAGKSDVNVAEVNVPVVG 203
>At2g09900.1 68415.m01028 hypothetical protein
Length = 175
Score = 31.9 bits (69), Expect = 0.37
Identities = 18/43 (41%), Positives = 27/43 (62%), Gaps = 1/43 (2%)
Frame = -2
Query: 417 KNIGVSLHEIETRFTFALSNTSSNHADLRASCY-RIIRTRLDL 292
++I SLH TR + +S T S+H+ +R+S + IIR LDL
Sbjct: 28 EDITTSLHHSTTRSSALISITRSHHSTMRSSVFTSIIRQPLDL 70
>At5g13230.1 68418.m01520 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 822
Score = 31.5 bits (68), Expect = 0.49
Identities = 19/66 (28%), Positives = 33/66 (50%), Gaps = 1/66 (1%)
Frame = +1
Query: 67 KMESLKKLFQPVHEKVDETWSKVTIVGVGQVGMAA-AFSMLTQNVTNNIALVDMMADKLK 243
KM++ KLF + K + +W+ V IVG +G AFSM + + N +++ ++
Sbjct: 398 KMDTAVKLFAELSSKNEVSWNTV-IVGYENLGEGGKAFSMFREALRNQVSVTEVTFSSAL 456
Query: 244 GEMMDL 261
G L
Sbjct: 457 GACASL 462
>At4g04720.1 68417.m00693 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calcium-dependent protein
kinase(CDPK) [Carrot] SWISS-PROT:P28582
Length = 531
Score = 29.1 bits (62), Expect = 2.6
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = -2
Query: 603 TEPVRQQVTEPCRVQVSAGPDDAVLGQP 520
T+P+ QQ++ P VS D +LG+P
Sbjct: 45 TQPIHQQISTPSSNPVSVRDPDTILGKP 72
>At2g41360.1 68415.m05105 F-box family protein similar to SKP1
interacting partner 6 [Arabidopsis thaliana]
GI:10716957; contains Pfam profile PF00646: F-box domain
Length = 373
Score = 29.1 bits (62), Expect = 2.6
Identities = 19/50 (38%), Positives = 28/50 (56%), Gaps = 1/50 (2%)
Frame = -2
Query: 558 VSAGPDDAVLGQPANLPRNIRQNIHGVTGDHQYCVRT-VLYQLRDYLFKN 412
+S PD+ VL A +PR +NI V+ + VRT LY++R L K+
Sbjct: 11 LSCLPDEMVLNCLARVPRRYYENISCVSVRLRSLVRTPELYRMRSLLHKD 60
>At5g19740.1 68418.m02347 peptidase M28 family protein ileal
peptidase I100 - Rattus norvegicus, EMBL:AF009921;
contains Pfam profiles PF04389: Peptidase family M28,
PF02225: PA domain
Length = 681
Score = 28.3 bits (60), Expect = 4.5
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = -1
Query: 142 QWSPCSKFRPPSRARAGTASSG 77
+W P SK+ PPS + GT +G
Sbjct: 209 EWFPASKWMPPSGVQVGTVYNG 230
>At2g36720.1 68415.m04505 PHD finger transcription factor, putative
Length = 1007
Score = 27.9 bits (59), Expect = 6.0
Identities = 12/35 (34%), Positives = 21/35 (60%)
Frame = -2
Query: 426 YLFKNIGVSLHEIETRFTFALSNTSSNHADLRASC 322
Y++ + GVSLHE T F+ +++++ DL C
Sbjct: 587 YIYTSNGVSLHEWATTFSHGRKYSANDNNDLCVIC 621
>At1g51460.1 68414.m05792 ABC transporter family protein similar to
SP|Q9UNQ0 ATP-binding cassette, sub-family G, member 2
(Placenta-specific ATP- binding cassette transporter)
(Breast cancer resistance protein) {Homo sapiens};
contains Pfam profile PF00005: ABC transporter
Length = 678
Score = 27.9 bits (59), Expect = 6.0
Identities = 11/35 (31%), Positives = 18/35 (51%)
Frame = +1
Query: 442 KYSPDTILVIASNPVDILTYVTWKISGLPKHRVIG 546
++ PD +V PV + Y W + G K+ +IG
Sbjct: 535 RFFPDLPMVFWRYPVSYINYGAWALQGAYKNEMIG 569
>At5g53460.1 68418.m06644 glutamate synthase [NADH], chloroplast,
putative similar to SP|Q03460 Glutamate synthase [NADH],
chloroplast precursor (EC 1.4.1.14) (NADH- GOGAT)
{Medicago sativa}
Length = 2208
Score = 27.5 bits (58), Expect = 7.9
Identities = 20/59 (33%), Positives = 30/59 (50%)
Frame = +1
Query: 121 TWSKVTIVGVGQVGMAAAFSMLTQNVTNNIALVDMMADKLKGEMMDLQHGSAFMRNAKI 297
T KV I+G G G+AAA + N ++ V +D++ G MM +G M+ KI
Sbjct: 1844 TGKKVAIIGSGPAGLAAADQL---NKMGHLVTVYERSDRIGGLMM---YGVPNMKTDKI 1896
>At4g07670.1 68417.m01203 protease-associated (PA) domain-containing
protein similar to PF02225: PA domain; similar to
N-acetylated-alpha-linked acidic dipeptidase II
(NAALADase II) (SP:Q9Y3Q) {Homo sapiens};similar to
Glutamate carboxypeptidase II (Membrane glutamate
carboxypeptidase) (mGCP) (N-acetylated-alpha-linked
acidic dipeptidase I) (NAALADase I)
(Pteroylpoly-gamma-glutamate
carboxypeptidase)(Folylpoly-gamma-glutamate
carboxypeptidase) (FGCP) (Folate hydrolase 1)
(Prostate-specific membrane antigen homolog) (SP|O77564)
{Sus scrofa}
Length = 280
Score = 27.5 bits (58), Expect = 7.9
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = -1
Query: 142 QWSPCSKFRPPSRARAGTASSG 77
+W P SK+ PPS + GT +G
Sbjct: 74 EWFPASKWMPPSGFQVGTVYNG 95
>At3g47030.1 68416.m05107 F-box family protein contains F-box domain
Pfam:PF00646
Length = 414
Score = 27.5 bits (58), Expect = 7.9
Identities = 16/58 (27%), Positives = 28/58 (48%)
Frame = -2
Query: 516 NLPRNIRQNIHGVTGDHQYCVRTVLYQLRDYLFKNIGVSLHEIETRFTFALSNTSSNH 343
NLP +I +I G+T + + T + Y+F V+L ++ R F + +NH
Sbjct: 330 NLPADIDVSIVGMTSAGEIVLATSHIRHPFYIFYYSTVTLAIVQLRIDFGIEAPEANH 387
>At3g07400.1 68416.m00882 lipase class 3 family protein contains
Pfam profile PF01764: Lipase
Length = 1003
Score = 27.5 bits (58), Expect = 7.9
Identities = 12/36 (33%), Positives = 20/36 (55%)
Frame = +1
Query: 1 ETQTLTNSKHYVTSPTER*LNHKMESLKKLFQPVHE 108
E LT+ + +T ER +H M+S + FQ +H+
Sbjct: 477 EYSKLTSVRSVITELRERLQSHSMKSYRSRFQRIHD 512
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,422,856
Number of Sequences: 28952
Number of extensions: 308942
Number of successful extensions: 957
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 920
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 957
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1314848736
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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