Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner10m13f
(648 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 300 9e-83
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 162 5e-41
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 158 7e-40
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 157 2e-39
SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase Shk1|Schizosacc... 28 1.3
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 1.8
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 2.3
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 3.1
SPAC922.06 |||short chain dehydrogenase|Schizosaccharomyces pomb... 26 4.1
SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase |Schizosaccha... 26 4.1
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 25 7.1
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 25 7.1
SPAC29A4.16 |hal4|sat4, ppk10|halotolerence protein 4|Schizosacc... 25 9.4
SPAC644.16 |||RNA-binding protein|Schizosaccharomyces pombe|chr ... 25 9.4
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 300 bits (737), Expect = 9e-83
Identities = 134/193 (69%), Positives = 156/193 (80%)
Frame = +1
Query: 70 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 249
MREIVH+QAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 250 PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 429
PRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVV
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120
Query: 430 RKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPKVSDTVV 609
R+E+E+CD LQGFQ KIREEYPDR+M T+SV P+PK SDTVV
Sbjct: 121 RREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVV 180
Query: 610 EPYNATLSVHQLV 648
EPYNATLS+HQLV
Sbjct: 181 EPYNATLSMHQLV 193
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 162 bits (393), Expect = 5e-41
Identities = 77/192 (40%), Positives = 111/192 (57%), Gaps = 2/192 (1%)
Frame = +1
Query: 70 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGK 243
MREI+ + GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 244 YVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD 423
YVPR+I VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 424 VVRKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPKVSDT 603
+R+ +++C LQGF ++ EY + +SV P+P+VS +
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180
Query: 604 VVEPYNATLSVH 639
VVEPYN+ L+ H
Sbjct: 181 VVEPYNSVLTTH 192
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 158 bits (383), Expect = 7e-40
Identities = 72/194 (37%), Positives = 120/194 (61%), Gaps = 3/194 (1%)
Frame = +1
Query: 73 REIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 252
REI+ LQAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 253 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDV 426
RAIL+DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D+
Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121
Query: 427 VRKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVP-SPKVSDT 603
+ +E++ D L+GF ++ + YP +I+ TYSV P S VSD
Sbjct: 122 IDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDV 181
Query: 604 VVEPYNATLSVHQL 645
VV+PYN+ L++ +L
Sbjct: 182 VVQPYNSLLALKRL 195
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 157 bits (380), Expect = 2e-39
Identities = 75/196 (38%), Positives = 112/196 (57%), Gaps = 6/196 (3%)
Frame = +1
Query: 70 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 231
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 232 SGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVD 411
GK+VPR+I VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++D
Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120
Query: 412 SVLDVVRKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPK 591
SVL+ +R+ +++C LQGF ++ EY + +SV P+P+
Sbjct: 121 SVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQ 180
Query: 592 VSDTVVEPYNATLSVH 639
VS +VVEPYN+ L+ H
Sbjct: 181 VSTSVVEPYNSVLTTH 196
>SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase
Shk1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 658
Score = 27.9 bits (59), Expect = 1.3
Identities = 23/84 (27%), Positives = 42/84 (50%)
Frame = +2
Query: 374 PRDTTQRVLSSLTRSSM*SAKNQNLAIAYRASNLHIPSVAAPGPVWAPSSSQRSVKSTPT 553
PR++T++ L S++ S S+ Q + +S L+ PS AP P + SSS T
Sbjct: 219 PRESTEKPLLSVSALSS-SSHLQPTSATSSSSRLY-PSRPAPTPPASSSSSPLLSSQTVK 276
Query: 554 ES*THTQ*SPRPKYQTLSSNHTMR 625
+ ++ P P + S+++ +R
Sbjct: 277 TTTSNASRQPSPLVSSKSTDNIIR 300
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 27.5 bits (58), Expect = 1.8
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = +1
Query: 373 AKGHYTEGAELVDSVLDVVRKESESCDCLQ 462
A+GH G ELV + D +RK+SE+ L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 2.3
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +1
Query: 109 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 210
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 3.1
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +1
Query: 304 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 405
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPAC922.06 |||short chain dehydrogenase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 258
Score = 26.2 bits (55), Expect = 4.1
Identities = 11/32 (34%), Positives = 19/32 (59%)
Frame = +2
Query: 446 LAIAYRASNLHIPSVAAPGPVWAPSSSQRSVK 541
LA+ Y + + +V APG +W+P+ +R K
Sbjct: 159 LAVRYGPLGIRV-NVCAPGTIWSPAWDERFKK 189
>SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 363
Score = 26.2 bits (55), Expect = 4.1
Identities = 11/19 (57%), Positives = 15/19 (78%)
Frame = +2
Query: 53 KITYTK*GKSFIYRPANVV 109
+I+YT G SFIYRP N++
Sbjct: 64 EISYT--GTSFIYRPGNII 80
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.4 bits (53), Expect = 7.1
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +1
Query: 325 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDC 456
RP +F G++ G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 255
Score = 25.4 bits (53), Expect = 7.1
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = -2
Query: 362 PAPDCPKTKLSGRKICPKGPERTESMVPGSKS 267
P+ PK L R I P GPE + + GS S
Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51
>SPAC29A4.16 |hal4|sat4, ppk10|halotolerence protein
4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 636
Score = 25.0 bits (52), Expect = 9.4
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = +2
Query: 464 ASNLHIPSVAAPGPVWAPSSSQRSVKSTPTES 559
A+N+H+ SVAA PS+ V +T T S
Sbjct: 172 AANVHVLSVAASPNPSTPSNGPAPVSTTATPS 203
>SPAC644.16 |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 422
Score = 25.0 bits (52), Expect = 9.4
Identities = 12/30 (40%), Positives = 15/30 (50%)
Frame = +2
Query: 467 SNLHIPSVAAPGPVWAPSSSQRSVKSTPTE 556
S +H S A PGP+ PS+ ST E
Sbjct: 241 SPMHTYSSAVPGPISVPSAPYGRASSTIAE 270
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,500,400
Number of Sequences: 5004
Number of extensions: 49160
Number of successful extensions: 160
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 146
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 152
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 291768710
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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