Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner10l06f
(589 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g35630.1 68418.m04253 glutamine synthetase (GS2) identical to... 113 8e-26
At5g37600.1 68418.m04529 glutamine synthetase, putative similar ... 112 2e-25
At1g66200.1 68414.m07514 glutamine synthetase, putative similar ... 112 2e-25
At1g48470.1 68414.m05418 glutamine synthetase, putative similar ... 111 3e-25
At5g16570.1 68418.m01939 glutamine synthetase, putative similar ... 111 3e-25
At3g17820.1 68416.m02272 glutamine synthetase (GS1) identical to... 106 9e-24
At5g37030.1 68418.m04441 tRNA-splicing endonuclease positive eff... 29 1.7
At3g15370.1 68416.m01949 expansin, putative (EXP12) similar to e... 29 3.0
At2g41590.1 68415.m05139 expressed protein similar to zinc finge... 29 3.0
At4g11540.1 68417.m01851 DC1 domain-containing protein contains ... 28 4.0
At5g49270.1 68418.m06098 phytochelatin synthetase-related contai... 28 5.3
At4g32880.1 68417.m04679 homeobox-leucine zipper transcription f... 28 5.3
At5g19280.1 68418.m02298 kinase associated protein phosphatase (... 27 7.0
At1g53180.1 68414.m06027 expressed protein 27 7.0
At1g32880.1 68414.m04051 importin alpha-1 subunit, putative simi... 27 7.0
At1g27080.1 68414.m03301 proton-dependent oligopeptide transport... 27 7.0
At3g63500.2 68416.m07153 expressed protein 27 9.3
At3g63500.1 68416.m07152 expressed protein 27 9.3
At1g18550.1 68414.m02314 kinesin motor protein-related contains ... 27 9.3
>At5g35630.1 68418.m04253 glutamine synthetase (GS2) identical to
glutamine synthetase, chloroplast precursor (glutamate--
ammonia ligase, GS2) [Arabidopsis thaliana]
SWISS-PROT:Q43127
Length = 430
Score = 113 bits (272), Expect = 8e-26
Identities = 59/140 (42%), Positives = 82/140 (58%), Gaps = 1/140 (0%)
Frame = +3
Query: 165 KIEDNPKILSGPVLT-NSPNAVLSKTLLSRYNDLPLPADKILATYIWIDGSGEHLRCKDR 341
K + N K+ VL S N+ +++ D +D+I+A YIWI GSG LR K R
Sbjct: 37 KKQSNNKVRGFRVLALQSDNSTVNRVETLLNLDTKPYSDRIIAEYIWIGGSGIDLRSKSR 96
Query: 342 TLNFIPKAPKDLPIWNFDGSSTNQADGHNSDTYLVPRAIYKDPFRRGNHILVMCDTYKYN 521
T+ + P +LP WN+DGSST QA G +S+ L P+AI++DPFR GN+ILV+CDT+
Sbjct: 97 TIEKPVEDPSELPKWNYDGSSTGQAPGEDSEVILYPQAIFRDPFRGGNNILVICDTWTPA 156
Query: 522 MEPTESNNRISCQEAYDKCK 581
EP +N R E + K
Sbjct: 157 GEPIPTNKRAKAAEIFSNKK 176
>At5g37600.1 68418.m04529 glutamine synthetase, putative similar to
glutamine synthetase, cytosolic isozyme (Glutamate--
ammonia ligase, GS1) [Lotus japonicus] SWISS-PROT:Q42899
Length = 356
Score = 112 bits (269), Expect = 2e-25
Identities = 52/98 (53%), Positives = 65/98 (66%)
Frame = +3
Query: 276 DKILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFDGSSTNQADGHNSDTYLVPRA 455
DKI+A YIW+ GSG +R K RTL P LP WN+DGSST QA G +S+ L P+A
Sbjct: 17 DKIIAEYIWVGGSGMDMRSKARTLPGPVTDPSQLPKWNYDGSSTGQAPGEDSEVILYPQA 76
Query: 456 IYKDPFRRGNHILVMCDTYKYNMEPTESNNRISCQEAY 569
I+KDPFRRGN+ILVMCD Y EP +N R + + +
Sbjct: 77 IFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAAKVF 114
>At1g66200.1 68414.m07514 glutamine synthetase, putative similar to
glutamine synthetase, cytosolic isozyme (Glutamate--
ammonia ligase, GS1) [Lotus japonicus] SWISS-PROT:Q42899
Length = 356
Score = 112 bits (269), Expect = 2e-25
Identities = 53/104 (50%), Positives = 68/104 (65%)
Frame = +3
Query: 258 DLPLPADKILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFDGSSTNQADGHNSDT 437
D+ ++KI+A YIW+ GSG +R K RTL P LP WN+DGSST QA G +S+
Sbjct: 11 DISDNSEKIIAEYIWVGGSGMDMRSKARTLPGPVTDPSKLPKWNYDGSSTGQAPGQDSEV 70
Query: 438 YLVPRAIYKDPFRRGNHILVMCDTYKYNMEPTESNNRISCQEAY 569
L P+AI+KDPFRRGN+ILVMCD Y EP +N R + E +
Sbjct: 71 ILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAAEIF 114
>At1g48470.1 68414.m05418 glutamine synthetase, putative similar to
glutamine synthetase, cytosolic isozyme (glutamate--
ammonia ligase, GS1) [Arabidopsis thaliana]
SWISS-PROT:Q9LVI8
Length = 353
Score = 111 bits (268), Expect = 3e-25
Identities = 52/98 (53%), Positives = 67/98 (68%)
Frame = +3
Query: 279 KILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFDGSSTNQADGHNSDTYLVPRAI 458
KI+A YIWI GSG +R K RTL P LP WN+DGSST+QA G +S+ L P+AI
Sbjct: 18 KIIAEYIWIGGSGMDIRSKARTLPGPVSNPTKLPKWNYDGSSTDQAAGDDSEVILYPQAI 77
Query: 459 YKDPFRRGNHILVMCDTYKYNMEPTESNNRISCQEAYD 572
+KDPFR+GN+ILVMCD Y+ +P +NNR + +D
Sbjct: 78 FKDPFRKGNNILVMCDAYRPAGDPIPTNNRHKAVKIFD 115
>At5g16570.1 68418.m01939 glutamine synthetase, putative similar to
glutamine synthetase, cytosolic isozyme (glutamate--
ammonia ligase) [Alfalfa] SWISS-PROT:P04078
Length = 356
Score = 111 bits (267), Expect = 3e-25
Identities = 53/105 (50%), Positives = 68/105 (64%)
Frame = +3
Query: 258 DLPLPADKILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFDGSSTNQADGHNSDT 437
DL D+I+A YIWI GSG +R K RTL P LP WN+DGSST QA G +S+
Sbjct: 11 DLSDSTDQIIAEYIWIGGSGLDMRSKARTLPGPVTDPSQLPKWNYDGSSTGQAPGDDSEV 70
Query: 438 YLVPRAIYKDPFRRGNHILVMCDTYKYNMEPTESNNRISCQEAYD 572
+ P+AI+KDPFRRGN+ILVMCD Y EP +N R + + ++
Sbjct: 71 IIYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAAKIFE 115
>At3g17820.1 68416.m02272 glutamine synthetase (GS1) identical to
glutamine synthetase, cytosolic isozyme (glutamate--
ammonia ligase, GS1) [Arabidopsis thaliana]
SWISS-PROT:Q9LVI8
Length = 354
Score = 106 bits (255), Expect = 9e-24
Identities = 50/90 (55%), Positives = 61/90 (67%)
Frame = +3
Query: 279 KILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFDGSSTNQADGHNSDTYLVPRAI 458
KI+A YIWI GSG +R K RTL P LP WN+DGSST QA G +S+ L P+AI
Sbjct: 18 KIIAEYIWIGGSGMDIRSKARTLPGPVTDPSKLPKWNYDGSSTGQAAGEDSEVILYPQAI 77
Query: 459 YKDPFRRGNHILVMCDTYKYNMEPTESNNR 548
+KDPFR+GN+ILVMCD Y +P +N R
Sbjct: 78 FKDPFRKGNNILVMCDAYTPAGDPIPTNKR 107
>At5g37030.1 68418.m04441 tRNA-splicing endonuclease positive
effector-related contains similarity to SEN1, a positive
effector of tRNA-splicing endonuclease [Saccharomyces
cerevisiae] gi|172574|gb|AAB63976
Length = 638
Score = 29.5 bits (63), Expect = 1.7
Identities = 17/80 (21%), Positives = 39/80 (48%)
Frame = -1
Query: 253 YRLSSVLDSTAFGELVKTGPDRIFGLSSILVCAVSAILKQVRHDYVLKCSLDASVEYCAK 74
+ + +L+ + + +KT PD+ ++ L C V +L++ R + L S + +K
Sbjct: 16 WSMEDILNKDLYKQKIKTIPDKFSSVNEYLKCFVPHLLEETRTE------LSTSFKSLSK 69
Query: 73 TYLRKVLSERKMFRSPHGAV 14
+L ++ S M + G++
Sbjct: 70 AHLFEISSLETMTKESSGSL 89
>At3g15370.1 68416.m01949 expansin, putative (EXP12) similar to
expansin GI:11191999 from [Lycopersicon esculentum];
alpha-expansin gene family, PMID:11641069
Length = 252
Score = 28.7 bits (61), Expect = 3.0
Identities = 16/49 (32%), Positives = 23/49 (46%)
Frame = -3
Query: 479 STEWILVNSTRYEVGIRIVPISLVG*AAIEVPYRQIFGSFRYEIQGAIF 333
S WI ++T Y G+ P SL G + PY FG+ + G +F
Sbjct: 24 SNGWIRAHATYY--GVNDSPASLGGACGYDNPYHAGFGAHTAALSGELF 70
>At2g41590.1 68415.m05139 expressed protein similar to zinc finger
protein [Arabidopsis thaliana] GI:976277
Length = 367
Score = 28.7 bits (61), Expect = 3.0
Identities = 13/36 (36%), Positives = 21/36 (58%), Gaps = 1/36 (2%)
Frame = +3
Query: 249 RYNDLPLPADKILATYIWID-GSGEHLRCKDRTLNF 353
R + PL D+ +A Y +D G +H+RC+ + NF
Sbjct: 263 RISHPPLNHDEFVAAYPHLDSGRNDHIRCEGESSNF 298
>At4g11540.1 68417.m01851 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 525
Score = 28.3 bits (60), Expect = 4.0
Identities = 19/63 (30%), Positives = 28/63 (44%)
Frame = -2
Query: 432 QNCAHQLGWLSCHRSSI*ADLWEL*V*NSRCDLCTSGVRQSRRSKCRWRESCQPAVEDHC 253
Q+ +H+ +L H + + LWE N RC CT VR C+ C + HC
Sbjct: 286 QHFSHKEHYLRFHVNGL---LWEE---NKRCSACTHPVRLQSFYACK---DCDFILHQHC 336
Query: 252 IDS 244
+S
Sbjct: 337 AES 339
>At5g49270.1 68418.m06098 phytochelatin synthetase-related contains
Pfam PF04833: Phytochelatin synthetase-like conserved
region
Length = 663
Score = 27.9 bits (59), Expect = 5.3
Identities = 11/23 (47%), Positives = 17/23 (73%)
Frame = -3
Query: 518 VFIRITHNEDVISSTEWILVNST 450
VF+ HNE +IS+T+ ++VN T
Sbjct: 91 VFVGFQHNEILISATDALIVNGT 113
>At4g32880.1 68417.m04679 homeobox-leucine zipper transcription
factor (HB-8) identical to HD-zip transcription factor
(athb-8) (GI:7270235) [Arabidopsis thaliana]
Length = 833
Score = 27.9 bits (59), Expect = 5.3
Identities = 14/34 (41%), Positives = 17/34 (50%)
Frame = +2
Query: 296 HLDRRLWRTPEVQRSHLEFHT*SSQRSAYMELRW 397
H D W PEV RS E T +QR+ LR+
Sbjct: 332 HFDLEPWSVPEVLRSLYESSTLLAQRTTMAALRY 365
>At5g19280.1 68418.m02298 kinase associated protein phosphatase
(KAPP) identical to Kinase associated protein
phosphatase (SP:P46014) [Arabidopsis thaliana]; contains
Pfam PF00481: Protein phosphatase 2C domain; contains
Pfam PF00498: FHA domain
Length = 581
Score = 27.5 bits (58), Expect = 7.0
Identities = 18/51 (35%), Positives = 26/51 (50%), Gaps = 7/51 (13%)
Frame = +3
Query: 261 LPLPADK----ILATYIWIDGSGEHLRC---KDRTLNFIPKAPKDLPIWNF 392
L LPA+K + TY W + E+L+ KDR +N P+ +D W F
Sbjct: 133 LKLPAEKGSLAEVQTYDWQNNRNENLQYNLEKDRLINLSPRLVEDQRSWLF 183
>At1g53180.1 68414.m06027 expressed protein
Length = 358
Score = 27.5 bits (58), Expect = 7.0
Identities = 16/39 (41%), Positives = 21/39 (53%)
Frame = -1
Query: 319 SPEPSIQM*VARILSAGSGRSLYRLSSVLDSTAFGELVK 203
SPE + ++L A +GRSL R SS+ DS G K
Sbjct: 49 SPEKMGDSDIGKVLEASTGRSLIRASSI-DSREGGSQTK 86
>At1g32880.1 68414.m04051 importin alpha-1 subunit, putative similar
to importin alpha-1 subunit (Karyopherin alpha-1
subunit, KAP alpha) [Arabidopsis thaliana]
SWISS-PROT:Q96321
Length = 183
Score = 27.5 bits (58), Expect = 7.0
Identities = 17/59 (28%), Positives = 26/59 (44%)
Frame = -1
Query: 292 VARILSAGSGRSLYRLSSVLDSTAFGELVKTGPDRIFGLSSILVCAVSAILKQVRHDYV 116
V R++ S +L SV+D+ LVK + F + VCA+S HD +
Sbjct: 26 VHRLIQFLLDESFPKLQSVIDANLIPTLVKLTQNAEFDMKKESVCAISNATLLGSHDQI 84
>At1g27080.1 68414.m03301 proton-dependent oligopeptide transport
(POT) family protein similar to nitrate transporter
NRT1-5 [Glycine max] GI:11933414; contains Pfam profile
PF00854: POT family
Length = 525
Score = 27.5 bits (58), Expect = 7.0
Identities = 16/42 (38%), Positives = 24/42 (57%)
Frame = +1
Query: 463 RIHSVEEITSSLCVIRINTTWSQQRATIASVVKKLTTNARMM 588
R+ S++E+ C+IR+ WS A I S+V +TT A M
Sbjct: 259 RLCSIQEVEEVKCLIRVVPVWS---AGIISIV-AMTTQATFM 296
>At3g63500.2 68416.m07153 expressed protein
Length = 1162
Score = 27.1 bits (57), Expect = 9.3
Identities = 10/28 (35%), Positives = 19/28 (67%)
Frame = -2
Query: 90 LNIARKRIYERFCRSVKCFDRLTGRSRD 7
LN AR+ +ERFC+ ++ +++ S+D
Sbjct: 911 LNFAREWKFERFCKELEYVNKIFSSSKD 938
>At3g63500.1 68416.m07152 expressed protein
Length = 887
Score = 27.1 bits (57), Expect = 9.3
Identities = 10/28 (35%), Positives = 19/28 (67%)
Frame = -2
Query: 90 LNIARKRIYERFCRSVKCFDRLTGRSRD 7
LN AR+ +ERFC+ ++ +++ S+D
Sbjct: 636 LNFAREWKFERFCKELEYVNKIFSSSKD 663
>At1g18550.1 68414.m02314 kinesin motor protein-related contains
similarity to kinesin-related protein GI:4493964 from
[Plasmodium falciparum]
Length = 799
Score = 27.1 bits (57), Expect = 9.3
Identities = 26/76 (34%), Positives = 36/76 (47%), Gaps = 5/76 (6%)
Frame = -1
Query: 349 FKVRSLHLRCSPEPSIQM*VARIL-SAGSGRSLYRLSSVLDSTAFGELVKTG----PDRI 185
F +R+ + SP+P Q ++L S+ G +RL DST E K DRI
Sbjct: 52 FSLRNPNHNQSPKPEDQRFKTQLLDSSKKGDRFHRLDGK-DSTFVEEDTKENNVFEADRI 110
Query: 184 FGLSSILVCAVSAILK 137
FG+SS+ V I K
Sbjct: 111 FGVSSVPVKPSGVIRK 126
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,493,067
Number of Sequences: 28952
Number of extensions: 300095
Number of successful extensions: 784
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 773
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 784
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1161268208
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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