Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner10k09f
(584 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g52800.1 68418.m06552 expressed protein ; expression supporte... 30 0.99
At1g08260.1 68414.m00911 DNA-directed DNA polymerase epsilon cat... 29 2.3
At3g43520.1 68416.m04614 expressed protein contains Pfam profile... 29 3.0
At5g20380.1 68418.m02424 transporter-related low similarity to v... 28 5.3
At1g50500.1 68414.m05664 membrane trafficking VPS53 family prote... 28 5.3
At3g27470.1 68416.m03433 expressed protein contains Pfam profile... 27 7.0
At1g53345.1 68414.m06047 expressed protein 27 7.0
At1g12760.1 68414.m01481 zinc finger (C3HC4-type RING finger) fa... 27 7.0
At5g64100.1 68418.m08050 peroxidase, putative identical to perox... 27 9.2
At2g33550.1 68415.m04112 gt-2-related weak similarity to gt-2 (G... 27 9.2
At2g20950.1 68415.m02471 expressed protein 27 9.2
At1g74670.1 68414.m08647 gibberellin-responsive protein, putativ... 27 9.2
>At5g52800.1 68418.m06552 expressed protein ; expression supported
by MPSS
Length = 618
Score = 30.3 bits (65), Expect = 0.99
Identities = 10/23 (43%), Positives = 17/23 (73%)
Frame = -3
Query: 129 IFTVLLLVVYIVDFRRRVYYSRC 61
I+ + + ++YIVD RR +YY +C
Sbjct: 482 IYDIRVSMMYIVDLRRGIYYQKC 504
>At1g08260.1 68414.m00911 DNA-directed DNA polymerase epsilon
catalytic subunit, putative similar to SP|Q07864 DNA
polymerase epsilon, catalytic subunit A (EC 2.7.7.7) (DNA
polymerase II subunit A) {Homo sapiens}; contains Pfam
profiles: PF03175 DNA polymerase type B, organellar and
viral, PF00136 DNA polymerase family B, PF03104 DNA
polymerase family B, exonuclease domain
Length = 2271
Score = 29.1 bits (62), Expect = 2.3
Identities = 15/78 (19%), Positives = 34/78 (43%)
Frame = +1
Query: 106 DKQQNCENSPQGHVFNEAQVIGTWHPQQHKSKKTYAFGDSECVQLTSVNEQERNELKEMI 285
+K+ +C ++ G +++ Q+ + + ++ Y D C++ V E E
Sbjct: 2168 EKEWSCADTQCGKIYDREQMESSLLEMVRQRERMYHMQDVVCIRCNQVKAAHLTEQCECS 2227
Query: 286 GNYVDKMKWENLTLRMQI 339
G++ K + RM+I
Sbjct: 2228 GSFRCKESGSEFSKRMEI 2245
>At3g43520.1 68416.m04614 expressed protein contains Pfam profile
PF03647: Uncharacterised protein family (UPF0136)
Length = 240
Score = 28.7 bits (61), Expect = 3.0
Identities = 13/42 (30%), Positives = 24/42 (57%)
Frame = -3
Query: 414 PVKTVALKTL*IIVFGYIMFVTRTRYLHSQS*VLPFHFVDVI 289
P K V T+ +++ G +M+V TRY+ S+ + P V ++
Sbjct: 183 PTKPVLASTVGVVMAGALMYVMGTRYMRSKK-IFPAGVVSIM 223
>At5g20380.1 68418.m02424 transporter-related low similarity to
vesicular glutamate transporter 3 [Rattus norvegicus]
GI:21685382
Length = 517
Score = 27.9 bits (59), Expect = 5.3
Identities = 11/31 (35%), Positives = 15/31 (48%)
Frame = -1
Query: 170 PITWASLKTWPCGEFSQFCCLSCTSLTFGDA 78
P WA + T CG + + CLS F +A
Sbjct: 317 PAVWAMIYTHFCGSWGHYTCLSWLPTYFSEA 347
>At1g50500.1 68414.m05664 membrane trafficking VPS53 family protein
contains Pfam domain PF04100: Vps53-like, N-terminal
Length = 798
Score = 27.9 bits (59), Expect = 5.3
Identities = 15/63 (23%), Positives = 31/63 (49%)
Frame = +1
Query: 85 PKVNDVHDKQQNCENSPQGHVFNEAQVIGTWHPQQHKSKKTYAFGDSECVQLTSVNEQER 264
PK+ ++ +K N + + HVF++ +GT + ++ DS C+ + ++ R
Sbjct: 157 PKITELREKLNNIKQILKSHVFSDFSSLGT-GKETEETNLLQKLSDS-CLVVDALEPSVR 214
Query: 265 NEL 273
EL
Sbjct: 215 EEL 217
>At3g27470.1 68416.m03433 expressed protein contains Pfam profile
PF05212: Protein of unknown function (DUF707)
Length = 398
Score = 27.5 bits (58), Expect = 7.0
Identities = 15/47 (31%), Positives = 21/47 (44%)
Frame = +1
Query: 262 RNELKEMIGNYVDKMKWENLTLRMQIPCPSDKHNVTKDYYLERLEGD 402
+N++ + NY D W + R P D D+YL RL GD
Sbjct: 76 KNDIPDPPYNYNDTKIWVSTNPRGAERLPPDIVTPESDFYLRRLWGD 122
>At1g53345.1 68414.m06047 expressed protein
Length = 325
Score = 27.5 bits (58), Expect = 7.0
Identities = 17/51 (33%), Positives = 27/51 (52%)
Frame = +1
Query: 178 HPQQHKSKKTYAFGDSECVQLTSVNEQERNELKEMIGNYVDKMKWENLTLR 330
H +S + GD E + +VN E EL+ +GN + + K +NL+LR
Sbjct: 215 HEVLEQSYEIVLGGDEEFGRCLAVNADEITELRSELGNQLAE-KSKNLSLR 264
>At1g12760.1 68414.m01481 zinc finger (C3HC4-type RING finger)
family protein low similarity to SP|Q9NVW2 RING finger
protein 12 (LIM domain interacting RING finger protein)
{Homo sapiens}; contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 368
Score = 27.5 bits (58), Expect = 7.0
Identities = 26/103 (25%), Positives = 41/103 (39%)
Frame = +1
Query: 88 KVNDVHDKQQNCENSPQGHVFNEAQVIGTWHPQQHKSKKTYAFGDSECVQLTSVNEQERN 267
K + D ++ + QG GT P +H T D+EC S E +
Sbjct: 270 KFRKLGDANKHTNDEAQGTTEGIMTECGTDSPIEH----TLLQEDAECCICLSAYE-DGT 324
Query: 268 ELKEMIGNYVDKMKWENLTLRMQIPCPSDKHNVTKDYYLERLE 396
EL+E+ + + L + CP K+N+ K L+R E
Sbjct: 325 ELRELPCGHHFHCSCVDKWLYINATCPLCKYNILKSSNLDREE 367
>At5g64100.1 68418.m08050 peroxidase, putative identical to
peroxidase ATP3a [Arabidopsis thaliana]
gi|1546698|emb|CAA67340
Length = 331
Score = 27.1 bits (57), Expect = 9.2
Identities = 22/63 (34%), Positives = 32/63 (50%)
Frame = +3
Query: 132 SARPRLQRSPGYWHMAPATTQIKENLRIRGLRMCSINFGQRTGKKRIKRNDRELRRQNEM 311
S+R LQ W P T I E R+ GLR S+ FG GK +K + E++ ++
Sbjct: 265 SSRVVLQSDLVLWK-DPETRAIIE--RLLGLRRPSLRFGTEFGKSMVKMSLIEVKTGSD- 320
Query: 312 GEL 320
GE+
Sbjct: 321 GEI 323
>At2g33550.1 68415.m04112 gt-2-related weak similarity to gt-2
(GI:20249) [Oryza sativa]
Length = 314
Score = 27.1 bits (57), Expect = 9.2
Identities = 32/125 (25%), Positives = 52/125 (41%), Gaps = 8/125 (6%)
Frame = +1
Query: 73 INASP---KVNDVHDKQ-QNCENSPQGHVFNE----AQVIGTWHPQQHKSKKTYAFGDSE 228
+N++P V DV DK+ Q + QG V + A V G Q+ + +K +FG+ E
Sbjct: 186 LNSTPVAKSVTDVIDKEKQEACVADQGRVKEKQPEAANVEGGSTSQEERKRKRTSFGEKE 245
Query: 229 CVQLTSVNEQERNELKEMIGNYVDKMKWENLTLRMQIPCPSDKHNVTKDYYLERLEGDGF 408
E+E E K+M ++ ++ L Q+ N+ D + GD
Sbjct: 246 -------EEEEEGETKKMQNQLIEILERNGQLLAAQLEV--QNLNLKLDREQRKDHGDSL 296
Query: 409 YRTLN 423
LN
Sbjct: 297 VAVLN 301
>At2g20950.1 68415.m02471 expressed protein
Length = 520
Score = 27.1 bits (57), Expect = 9.2
Identities = 12/34 (35%), Positives = 17/34 (50%)
Frame = +1
Query: 328 RMQIPCPSDKHNVTKDYYLERLEGDGFYRTLNMP 429
R + P P + H +DY ER E +G + N P
Sbjct: 151 RSRSPAPYNTHERGRDYSRERYEAEGNVTSRNRP 184
>At1g74670.1 68414.m08647 gibberellin-responsive protein, putative
similar to SP|P46690 Gibberellin-regulated protein 4
precursor {Arabidopsis thaliana} GASA4; contains Pfam
profile PF02704: Gibberellin regulated protein
Length = 101
Score = 27.1 bits (57), Expect = 9.2
Identities = 12/32 (37%), Positives = 16/32 (50%), Gaps = 3/32 (9%)
Frame = -1
Query: 140 PCGEFSQFCCLSCTSL---TFGDAFITPAANN 54
PC F Q CC C + T+G+ + P NN
Sbjct: 59 PCMFFCQKCCAKCLCVPPGTYGNKQVCPCYNN 90
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,953,632
Number of Sequences: 28952
Number of extensions: 278868
Number of successful extensions: 829
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 808
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 829
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1151426952
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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