Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner10j18r
(735 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g12230.1 68414.m01415 transaldolase, putative similar to Swis... 85 5e-17
At1g71970.1 68414.m08319 expressed protein 30 1.4
At1g76340.1 68414.m08869 integral membrane family protein contai... 29 2.4
At4g35410.1 68417.m05029 clathrin adaptor complex small chain fa... 29 4.2
At1g70390.1 68414.m08097 F-box family protein contains Pfam prof... 28 5.6
At2g23450.2 68415.m02800 protein kinase family protein contains ... 27 9.8
At2g23450.1 68415.m02799 protein kinase family protein contains ... 27 9.8
At2g18170.1 68415.m02114 mitogen-activated protein kinase, putat... 27 9.8
>At1g12230.1 68414.m01415 transaldolase, putative similar to
Swiss-Prot:P30148 transaldolase B (EC 2.2.1.2)
[Escherichia coli O157:H7]
Length = 405
Score = 85.0 bits (201), Expect = 5e-17
Identities = 46/121 (38%), Positives = 71/121 (58%), Gaps = 5/121 (4%)
Frame = -1
Query: 735 IQAAKELEKKHGIHCNLTLLFSLYQAIACAEANVTLISPFVGRILDWYVEHTKKT----- 571
I+AA+ LE + GI ++T ++S QA A ++A ++I FVGR+ DW H+ T
Sbjct: 209 IEAARLLESE-GIQTHMTFVYSFAQAAAASQAGASVIQIFVGRLRDWARNHSGDTEIESA 267
Query: 570 YEGKEDPGVVSVTKIYNYYKKFGYKTQVMGASFRNTGEIRELAGCDLLTISPKLLQELAS 391
+ EDPG+ V + YNY K+GYK+++M A+ RN ++ L G D + K+LQ L
Sbjct: 268 IKSGEDPGLALVKRSYNYIHKYGYKSKLMAAAVRNKQDLFSLLGVDYVIAPLKVLQSLKD 327
Query: 390 S 388
S
Sbjct: 328 S 328
>At1g71970.1 68414.m08319 expressed protein
Length = 225
Score = 30.3 bits (65), Expect = 1.4
Identities = 21/70 (30%), Positives = 32/70 (45%), Gaps = 1/70 (1%)
Frame = +2
Query: 287 SLRCQRNSDSVSDIFSMSFKAANFGSRTFFSGLSLLA-NSCSNLGLIVSKSQPANSRISP 463
SLR RN SD + K++ R+ S S + +SCS+ L+ P NS +P
Sbjct: 3 SLRT-RNVSKRSDPINFPAKSSISSRRSLSSSSSFSSFSSCSSSSLVFPGDSPLNSPATP 61
Query: 464 VLRKDAPITW 493
+ P +W
Sbjct: 62 LRLLGVPFSW 71
>At1g76340.1 68414.m08869 integral membrane family protein contains
Pfam profile PF00892: Integral membrane protein;
similar to GDP-mannose transporter (SP:Q941R4)
[Arabidopsis thaliana] and to LPG2 protein (GI:9998817)
[Leishmania mexicana]
Length = 372
Score = 29.5 bits (63), Expect = 2.4
Identities = 17/45 (37%), Positives = 25/45 (55%), Gaps = 2/45 (4%)
Frame = +1
Query: 490 LGLVTKLLVIVVNFCHGNYSGVFLALISLL-CMF-NIPIQYTTNK 618
LG+V KLL +V+N + F+ + LL CMF + Q +T K
Sbjct: 284 LGIVNKLLTVVINLMVWDKHSTFVGTLGLLVCMFGGVMYQQSTIK 328
>At4g35410.1 68417.m05029 clathrin adaptor complex small chain
family protein contains Pfam profile: PF01217 clathrin
adaptor complex small chain
Length = 124
Score = 28.7 bits (61), Expect = 4.2
Identities = 14/35 (40%), Positives = 22/35 (62%)
Frame = -1
Query: 717 LEKKHGIHCNLTLLFSLYQAIACAEANVTLISPFV 613
L++ G C L L+F+ ++ IAC + + TL PFV
Sbjct: 91 LDRYFGSVCELDLIFNFHKVIACCKHSRTL--PFV 123
>At1g70390.1 68414.m08097 F-box family protein contains Pfam
profile: PF00646 F-box domain
Length = 332
Score = 28.3 bits (60), Expect = 5.6
Identities = 19/54 (35%), Positives = 28/54 (51%), Gaps = 4/54 (7%)
Frame = +3
Query: 453 VFPRYYGKMHPLLGSCNQ----TSCNSCKFLSRKLLRGLPCPHKSSLYVQHTNP 602
VF Y + PLL SC+Q + N+ +F++ + + GL C S V H NP
Sbjct: 73 VFHTLYQEEEPLLSSCHQQPMRITNNAPRFIASQPILGLICLRNGSEVVIH-NP 125
>At2g23450.2 68415.m02800 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 708
Score = 27.5 bits (58), Expect = 9.8
Identities = 19/69 (27%), Positives = 35/69 (50%), Gaps = 2/69 (2%)
Frame = +2
Query: 215 DSSFLVSAANRLIPSESLSVAM*SSLRCQRNSDSVSDI--FSMSFKAANFGSRTFFSGLS 388
++SFL+ NR S+ ++ ++ D+ +I FS+ +++ S FFS +
Sbjct: 122 ENSFLMENCNRTTDGCSIKQKFLENVLKLKSCDATGNISCFSLDSNSSSKNSAKFFSMKT 181
Query: 389 LLANSCSNL 415
L +SCS L
Sbjct: 182 LRNSSCSLL 190
>At2g23450.1 68415.m02799 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 708
Score = 27.5 bits (58), Expect = 9.8
Identities = 19/69 (27%), Positives = 35/69 (50%), Gaps = 2/69 (2%)
Frame = +2
Query: 215 DSSFLVSAANRLIPSESLSVAM*SSLRCQRNSDSVSDI--FSMSFKAANFGSRTFFSGLS 388
++SFL+ NR S+ ++ ++ D+ +I FS+ +++ S FFS +
Sbjct: 122 ENSFLMENCNRTTDGCSIKQKFLENVLKLKSCDATGNISCFSLDSNSSSKNSAKFFSMKT 181
Query: 389 LLANSCSNL 415
L +SCS L
Sbjct: 182 LRNSSCSLL 190
>At2g18170.1 68415.m02114 mitogen-activated protein kinase, putative
/ MAPK, putative (MPK7) identical to mitogen-activated
protein kinase homolog 7 (AtMPK7)[Arabidopsis thaliana]
SWISS-PROT:Q39027; PMID:12119167
Length = 368
Score = 27.5 bits (58), Expect = 9.8
Identities = 12/42 (28%), Positives = 22/42 (52%)
Frame = +3
Query: 477 MHPLLGSCNQTSCNSCKFLSRKLLRGLPCPHKSSLYVQHTNP 602
+H ++ S S + CK+ +LLRGL H +++ + P
Sbjct: 120 LHQIIKSSQSLSDDHCKYFLFQLLRGLKYLHSANILHRDLKP 161
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,048,929
Number of Sequences: 28952
Number of extensions: 295805
Number of successful extensions: 769
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 744
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 768
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1614253080
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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