Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner10j08r
(764 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g14185.1 68414.m01678 glucose-methanol-choline (GMC) oxidored... 44 1e-04
At5g51930.1 68418.m06442 glucose-methanol-choline (GMC) oxidored... 40 0.001
At1g14190.1 68414.m01679 glucose-methanol-choline (GMC) oxidored... 39 0.003
At1g12570.1 68414.m01459 glucose-methanol-choline (GMC) oxidored... 39 0.003
At5g51950.1 68418.m06447 glucose-methanol-choline (GMC) oxidored... 39 0.004
At3g56060.1 68416.m06229 glucose-methanol-choline (GMC) oxidored... 39 0.004
At1g73050.1 68414.m08447 (R)-mandelonitrile lyase, putative / (R... 33 0.28
At1g04030.1 68414.m00390 expressed protein 32 0.48
At3g29580.1 68416.m03717 hypothetical protein weak similarity to... 29 2.6
At1g51965.1 68414.m05859 pentatricopeptide (PPR) repeat-containi... 29 2.6
At1g07330.1 68414.m00781 hypothetical protein 29 3.4
At4g02570.1 68417.m00351 cullin family protein similar to cullin... 29 4.5
At4g30870.1 68417.m04383 repair endonuclease family protein cont... 28 5.9
At4g03190.1 68417.m00436 F-box family protein (FBL18) almost ide... 28 7.8
>At1g14185.1 68414.m01678 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 503
Score = 43.6 bits (98), Expect = 1e-04
Identities = 45/154 (29%), Positives = 66/154 (42%), Gaps = 1/154 (0%)
Frame = -1
Query: 551 PKSAGRLLLKTKNPFDKPLLYADYYKDIEDLYTVIRAFKQHSLRLGETKAFKESGFKLEW 372
PKS GRL L NP + P + +Y ++ DL A ++ L L K F L
Sbjct: 364 PKSKGRLKLNNTNPRENPSVTFNYLENKADL----DACQEMVLHLQHVARSKTVTFFLGT 419
Query: 371 IELDACKSFDKNSDEFLECIAREITFSLYHPTSTVKMGADGDPTSVVDTKLRVRNVTGLR 192
D + D+ +F CI T+ YH V SVV+ + +V V LR
Sbjct: 420 QAQDKLVAGDEELKKF--CIKNVRTYYHYHGGCVV--------GSVVNEEYKVNGVKRLR 469
Query: 191 VMDASIMPSVIRGNTNAPSIMIGE-KGADMIKKH 93
V+D S N A +M+G +G ++K+H
Sbjct: 470 VVDGSTFEESPGTNPMATVLMLGRYQGIKILKEH 503
>At5g51930.1 68418.m06442 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 582
Score = 40.3 bits (90), Expect = 0.001
Identities = 37/155 (23%), Positives = 62/155 (40%), Gaps = 4/155 (2%)
Frame = -1
Query: 575 LVYNTLLKPKSAGRLLLKTKNPFDKPLLYADYYKDIEDLYTVIRAFKQHSLRLGETKAFK 396
L++ + P S G + L+ NP D P + +YY++ EDL ++ +R+ +KAF
Sbjct: 429 LIFQKVDGPASKGHMKLRNTNPRDNPSVTFNYYQEPEDLNKCVKGLNT-IIRMINSKAFS 487
Query: 395 ESGF----KLEWIELDACKSFDKNSDEFLECIAREITFSLYHPTSTVKMGADGDPTSVVD 228
+ + E + L + + F + TS VVD
Sbjct: 488 KYKYPGVTARELLNLMLALPINLRPRHVTSAFNLK-QFCIDTVTSVWHYHGGCQVGKVVD 546
Query: 227 TKLRVRNVTGLRVMDASIMPSVIRGNTNAPSIMIG 123
+V + GLRV+D S N A +M+G
Sbjct: 547 KNYKVLGIDGLRVIDGSTFLKSPGTNPQATVMMLG 581
>At1g14190.1 68414.m01679 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 501
Score = 39.1 bits (87), Expect = 0.003
Identities = 44/153 (28%), Positives = 64/153 (41%), Gaps = 1/153 (0%)
Frame = -1
Query: 551 PKSAGRLLLKTKNPFDKPLLYADYYKDIEDLYTVIRAFKQHSLRLGETKAFKESGFKLEW 372
PKS GRL L + NP + P + +Y ++ DL A + L L + F L
Sbjct: 344 PKSKGRLKLNSTNPRENPSVKFNYLENKADL----DACLEMVLHLQHVARSETVTFFLGT 399
Query: 371 IELDACKSFDKNSDEFLECIAREITFSLYHPTSTVKMGADGDPTSVVDTKLRVRNVTGLR 192
D + D+ F CI T+ YH V SVVD + +V V LR
Sbjct: 400 QAHDKLVAGDEELKSF--CIKNVRTYYHYHGGCVV--------GSVVDEEYKVNGVKRLR 449
Query: 191 VMDASIMPSVIRGNTNAPSIMIGE-KGADMIKK 96
V+D S N A +M+G +G ++K+
Sbjct: 450 VVDGSTFEESPGTNPMATVLMLGRYQGIKILKE 482
>At1g12570.1 68414.m01459 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 572
Score = 39.1 bits (87), Expect = 0.003
Identities = 20/54 (37%), Positives = 33/54 (61%)
Frame = -1
Query: 560 LLKPKSAGRLLLKTKNPFDKPLLYADYYKDIEDLYTVIRAFKQHSLRLGETKAF 399
++ P S G L LKT+NP D P++ +Y++ +DL +R Q R+ ++KAF
Sbjct: 406 VMGPLSTGHLELKTRNPKDNPIVTFNYFQHPDDLKRCVRGI-QTIERVVQSKAF 458
>At5g51950.1 68418.m06447 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 586
Score = 38.7 bits (86), Expect = 0.004
Identities = 42/168 (25%), Positives = 68/168 (40%), Gaps = 9/168 (5%)
Frame = -1
Query: 599 MNENKYTMLVYNTLLKPKSAGRLLLKTKNPFDKPLLYADYYKDIEDLYTVIRAFKQHSLR 420
+N L+ + P S G L L+ NP D P + +YY++ EDL + ++
Sbjct: 405 LNATTRAGLILQKIAGPISRGHLELRNTNPDDNPSVRFNYYQEPEDLQICVEGINT-IIK 463
Query: 419 LGETKAFKESGFKLEWIE--LDACKSFDKN------SDEF-LECIAREITFSLYHPTSTV 267
+ +KAF + + I LD S N + F L + +++H
Sbjct: 464 VINSKAFSKFKYPDATIHGLLDLMLSVPTNLRPRHITSMFNLRQFCIDTVMTIWHYHGGC 523
Query: 266 KMGADGDPTSVVDTKLRVRNVTGLRVMDASIMPSVIRGNTNAPSIMIG 123
++G VVD RV + LRV+D S N A +M+G
Sbjct: 524 QVG------RVVDKNYRVLGIDSLRVIDGSTFLKSPGTNPQATVMMLG 565
>At3g56060.1 68416.m06229 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 577
Score = 38.7 bits (86), Expect = 0.004
Identities = 45/193 (23%), Positives = 79/193 (40%), Gaps = 14/193 (7%)
Frame = -1
Query: 659 LLDMFRKHGLSEEVHDKFRK-----MNENKYTMLVYNTLLKPKSAGRLLLKTKNPFDKPL 495
+L++ +K L + KF K +N ++ + P S G L L+ NP D P
Sbjct: 371 VLNLLKKTKLPTQSISKFFKSLDLTLNVTTKAGVIIQKVNGPLSRGHLELRNTNPDDNPS 430
Query: 494 LYADYYKDIEDLYTVIRAFKQHSLRLGETKAFKESGFKLEWIE--LDACKSFDKN----- 336
+ +Y+KD EDL + +++ ++K + + + L L+ + N
Sbjct: 431 VTFNYFKDPEDLNKCVEGLST-IIKVIDSKGYSKYKYPLASARGLLNLILALPTNLRPRH 489
Query: 335 --SDEFLECIAREITFSLYHPTSTVKMGADGDPTSVVDTKLRVRNVTGLRVMDASIMPSV 162
S LE + ++YH ++G VVD +V V LR++D S
Sbjct: 490 ITSTFDLEQYCIDTVMTIYHYHGGCQVG------KVVDNNYKVLGVDALRIIDGSTFLKS 543
Query: 161 IRGNTNAPSIMIG 123
N A +M+G
Sbjct: 544 PGTNPQATIMMLG 556
>At1g73050.1 68414.m08447 (R)-mandelonitrile lyase, putative /
(R)-oxynitrilase, putative similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|52707];
contains Pfam protile PF00732 GMC oxidoreductase
Length = 552
Score = 32.7 bits (71), Expect = 0.28
Identities = 37/166 (22%), Positives = 72/166 (43%), Gaps = 7/166 (4%)
Frame = -1
Query: 572 VYNTLLKPKSAGRLLLKTKNPFDKPLLYADYYKDIEDLYTVIRAFKQHSLRLGETKAFKE 393
+ +L P S G L L + + P++ +Y+ D +DL + ++ L ++A ++
Sbjct: 392 IMEKILGPVSIGLLRLASTDVRINPVVRFNYFSDPQDLERCVNGTRKIGEIL-RSRAMQD 450
Query: 392 SGFKLEWIE------LDACKSFDKNSDEFLECIAREITFSLYHPTSTVKMGADGDPTSVV 231
+ EW + A D+++D + R +++H +G VV
Sbjct: 451 FMIR-EWFGNRRFRFVGAPLPVDQSNDLVMADFCRRTVSTIWHYHGGAVVG------KVV 503
Query: 230 DTKLRVRNVTGLRVMDASIMPSVIRGNTNAPSIMIGE-KGADMIKK 96
D+ L+V V LR++D S N A +M+G G M+++
Sbjct: 504 DSDLKVIGVNSLRLVDGSTFNISPGTNPQATLMMLGRYMGLKMLRE 549
>At1g04030.1 68414.m00390 expressed protein
Length = 418
Score = 31.9 bits (69), Expect = 0.48
Identities = 24/69 (34%), Positives = 31/69 (44%)
Frame = -1
Query: 407 KAFKESGFKLEWIELDACKSFDKNSDEFLECIAREITFSLYHPTSTVKMGADGDPTSVVD 228
K +ES +E E D C D + DE SL++PT V GD T +D
Sbjct: 125 KNCRESDDDIEEDEFD-CSDSDLDEDEEYYSDVGFSEDSLHNPTKEVYTQDIGDKTEEID 183
Query: 227 TKLRVRNVT 201
+KLR N T
Sbjct: 184 SKLRRSNET 192
>At3g29580.1 68416.m03717 hypothetical protein weak similarity to
ubiquitin-specific protease 12 [Arabidopsis thaliana]
GI:11993471
Length = 306
Score = 29.5 bits (63), Expect = 2.6
Identities = 15/40 (37%), Positives = 23/40 (57%), Gaps = 1/40 (2%)
Frame = -1
Query: 416 GETKAFKESGFKLEWIELDACKSFD-KNSDEFLECIAREI 300
G ++ KE+GFKL+W+E + + K +E E REI
Sbjct: 243 GLLRSMKEAGFKLDWLEKKLNEVLEKKEKEESYETRMREI 282
>At1g51965.1 68414.m05859 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 650
Score = 29.5 bits (63), Expect = 2.6
Identities = 20/58 (34%), Positives = 30/58 (51%), Gaps = 5/58 (8%)
Frame = -1
Query: 668 SYN-LLDMFRKHGLSEEVHDKFRKMNENKYT--MLVYNTL--LKPKSAGRLLLKTKNP 510
+YN LLD K+G + E D + KM + T + Y L L+ S G+ ++ KNP
Sbjct: 584 TYNILLDCLEKNGRTAEAVDLYSKMKQQGLTPDSITYTVLERLQSVSHGKSRIRRKNP 641
>At1g07330.1 68414.m00781 hypothetical protein
Length = 685
Score = 29.1 bits (62), Expect = 3.4
Identities = 13/25 (52%), Positives = 17/25 (68%)
Frame = -1
Query: 572 VYNTLLKPKSAGRLLLKTKNPFDKP 498
+ + L P+SA +LL TKNPFD P
Sbjct: 227 IVDGLQMPESAPSVLLPTKNPFDIP 251
>At4g02570.1 68417.m00351 cullin family protein similar to cullin 3
[Homo sapiens] GI:3639052; contains Pfam profile
PF00888: Cullin family
Length = 738
Score = 28.7 bits (61), Expect = 4.5
Identities = 12/43 (27%), Positives = 25/43 (58%)
Frame = -1
Query: 719 NSPASDMQYHYLIFPPSSYNLLDMFRKHGLSEEVHDKFRKMNE 591
N PA D + + +++ + YN+ H S++++DK+R+ E
Sbjct: 30 NEPAFDSEQYMMLYT-TIYNMCTQKPPHDYSQQLYDKYREAFE 71
Score = 28.3 bits (60), Expect = 5.9
Identities = 19/71 (26%), Positives = 33/71 (46%), Gaps = 1/71 (1%)
Frame = -1
Query: 638 HGLSEEVHDKFRKMNENKYTMLVYNTLLKPKSAG-RLLLKTKNPFDKPLLYADYYKDIED 462
H L K + +++ ++ + LL+ + +G R LL+ D +Y Y+K +
Sbjct: 237 HYLHSSSEPKLVEKVQHELLVVFASQLLEKEHSGCRALLRDDKVDDLSRMYRLYHKILRG 296
Query: 461 LYTVIRAFKQH 429
L V FKQH
Sbjct: 297 LEPVANIFKQH 307
>At4g30870.1 68417.m04383 repair endonuclease family protein
contains Pfam PF02732 : ERCC4 domain; similar to repair
endonuclease (TIGR:At5g41150) [Arabidopsis thaliana]
Length = 661
Score = 28.3 bits (60), Expect = 5.9
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = -3
Query: 246 PDLRSGHEIEGSKRYRPQSNGRKY 175
PD R+G + G KRY PQ N Y
Sbjct: 92 PDNRAGKKANGKKRYIPQRNSVGY 115
>At4g03190.1 68417.m00436 F-box family protein (FBL18) almost
identical to GRR1-like protein 1 GI:12658970 from
[Arabidopsis thaliana]; similar to leucine-rich repeats
containing F-box protein FBL3 (GI:5919219) [Homo
sapiens]; similar to F-box protein FBL2 (GI:6063090)
[Homo sapiens]
Length = 585
Score = 27.9 bits (59), Expect = 7.8
Identities = 12/27 (44%), Positives = 20/27 (74%), Gaps = 1/27 (3%)
Frame = +3
Query: 75 GVRVQPVLLDHVSSFL-TDHDRRSVSI 152
G+R P +L+H+ SF+ ++ DR SVS+
Sbjct: 2 GLRFPPKVLEHILSFIDSNEDRNSVSL 28
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,452,185
Number of Sequences: 28952
Number of extensions: 389025
Number of successful extensions: 1150
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 1102
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1148
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1712086600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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