Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner10j08f
(616 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g73050.1 68414.m08447 (R)-mandelonitrile lyase, putative / (R... 40 0.002
At5g51930.1 68418.m06442 glucose-methanol-choline (GMC) oxidored... 35 0.049
At1g14190.1 68414.m01679 glucose-methanol-choline (GMC) oxidored... 34 0.065
At1g14185.1 68414.m01678 glucose-methanol-choline (GMC) oxidored... 33 0.11
At5g18720.1 68418.m02221 hypothetical protein predicted proteins... 29 1.9
At5g51950.1 68418.m06447 glucose-methanol-choline (GMC) oxidored... 29 2.4
At5g23720.1 68418.m02780 dual specificity protein phosphatase fa... 29 3.2
At1g12570.1 68414.m01459 glucose-methanol-choline (GMC) oxidored... 28 4.3
At3g02065.2 68416.m00170 DEAD/DEAH box helicase family protein c... 27 7.5
At3g02065.1 68416.m00171 DEAD/DEAH box helicase family protein c... 27 7.5
At4g32910.1 68417.m04683 expressed protein 27 9.9
At2g18950.1 68415.m02212 homogentisate phytylprenyltransferase f... 27 9.9
At2g05120.1 68415.m00539 expressed protein 27 9.9
>At1g73050.1 68414.m08447 (R)-mandelonitrile lyase, putative /
(R)-oxynitrilase, putative similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|52707];
contains Pfam protile PF00732 GMC oxidoreductase
Length = 552
Score = 39.5 bits (88), Expect = 0.002
Identities = 35/115 (30%), Positives = 52/115 (45%)
Frame = +1
Query: 211 ADATDKVLEDPNYDFIXXXXXXXXXXXXNRLSEISDWKVLLVEAGGNPTLATEIPQPYYS 390
++ATD ED YD+I LS+ ++VLL+E GG P + +
Sbjct: 43 SNATDFASED-YYDYIIVGGGTAGCPLAATLSQ--SFRVLLLERGGVPYNRPNV-MSHDG 98
Query: 391 NMGTSEDWAYHTEPQEGACRAYKNKGCAWPRGKVLGGSSSINLMFYVRGNKADYD 555
+ T D P + +G RG+VLGGSS+IN FY R +K ++
Sbjct: 99 FLTTLTDVNNFDSPAQSFI---SEEGVPNARGRVLGGSSAINAGFYSRADKQFFE 150
>At5g51930.1 68418.m06442 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 582
Score = 34.7 bits (76), Expect = 0.049
Identities = 30/92 (32%), Positives = 40/92 (43%)
Frame = +1
Query: 325 VLLVEAGGNPTLATEIPQPYYSNMGTSEDWAYHTEPQEGACRAYKNKGCAWPRGKVLGGS 504
VL++E GG+P P ++MG S + + P + G R +VLGG
Sbjct: 97 VLVLERGGSP-----YENPTATDMGNSVNTLLNNTPNSWSQLFISEDGVYNTRPRVLGGG 151
Query: 505 SSINLMFYVRGNKADYDEWAADGNEGWSFEDV 600
S IN FY R DY E A W E+V
Sbjct: 152 SVINGGFYSRAGN-DYVEEAE-----WEMEEV 177
>At1g14190.1 68414.m01679 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 501
Score = 34.3 bits (75), Expect = 0.065
Identities = 37/133 (27%), Positives = 59/133 (44%), Gaps = 7/133 (5%)
Frame = +1
Query: 208 PADATD-KVLEDPNYDFIXXXXXXXXXXXXNRLSEISDWKVLLVEAGGNPTLATEIPQPY 384
P TD K + ++D+I LSE + VL++E GG+P + +
Sbjct: 2 PYMTTDAKEVSGKSFDYIVVGGGTAGCSLAATLSE--KYSVLVIERGGSPFGDPLVEERK 59
Query: 385 YSNMGTSEDWAYHTEPQEGACRAYKN-KGCAWPRGKVLGGSSSINLMFYVRGN-----KA 546
Y G S +T+ +++ + G RG+VLGGSS+IN FY R + KA
Sbjct: 60 Y--FGYS---LLNTDEYSSVAQSFTSVDGIENYRGRVLGGSSAINGGFYSRASDEFVKKA 114
Query: 547 DYDEWAADGNEGW 585
+D+ + W
Sbjct: 115 GWDKGLVQESYKW 127
>At1g14185.1 68414.m01678 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 503
Score = 33.5 bits (73), Expect = 0.11
Identities = 33/113 (29%), Positives = 51/113 (45%), Gaps = 2/113 (1%)
Frame = +1
Query: 208 PADATD-KVLEDPNYDFIXXXXXXXXXXXXNRLSEISDWKVLLVEAGGNPTLATEIPQPY 384
P TD K + ++D+I LSE + VL++E GG+P +
Sbjct: 23 PYMTTDPKEVSGKSFDYIVVGGGTAGCSLAATLSE--KYSVLVIERGGSPFGDPLVEDKK 80
Query: 385 YSNMGTSEDWAYHTEPQEGACRAYKN-KGCAWPRGKVLGGSSSINLMFYVRGN 540
Y G S +T+ +++ + G RG+VLGGSS+IN FY R +
Sbjct: 81 Y--YGYS---LINTDEYSSVAQSFTSVDGIKNHRGRVLGGSSAINGGFYSRAS 128
>At5g18720.1 68418.m02221 hypothetical protein predicted proteins -
Arabidopsis thaliana
Length = 552
Score = 29.5 bits (63), Expect = 1.9
Identities = 16/62 (25%), Positives = 30/62 (48%)
Frame = +2
Query: 344 VVILHWQPRYRNLTTVIWAHQKTGLTILNHRKGLVVPTKTRDALGHVVRCSAEAAASI*C 523
++I + +P + + W + +++ N R G TK R HV+ C+ + AS C
Sbjct: 126 LMITYLEPDSNDEKELQWFEKDLPVSVGNFRLGENKSTKDRSIFSHVIHCNERSNAS--C 183
Query: 524 FT 529
F+
Sbjct: 184 FS 185
>At5g51950.1 68418.m06447 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 586
Score = 29.1 bits (62), Expect = 2.4
Identities = 29/88 (32%), Positives = 38/88 (43%), Gaps = 6/88 (6%)
Frame = +1
Query: 325 VLLVEAGG----NPTLATEIPQPYYSNMGTSEDWAYHTEPQEGACRAYKNKGCAWPRGKV 492
VL++E GG NPT AT+I N T+ +T P+ + G R +V
Sbjct: 70 VLVLERGGAPYDNPT-ATDI-----ENFATTLS---NTSPKSWSQLFISEDGVYNTRARV 120
Query: 493 LGGSSSINLMFYVRGNK--ADYDEWAAD 570
LGG S +N FY R EW D
Sbjct: 121 LGGGSVLNAGFYTRAGDEYVKETEWKTD 148
>At5g23720.1 68418.m02780 dual specificity protein phosphatase
family protein contains Pfam profile: PF00782 dual
specificity phosphatase, catalytic domain; contains TG
acceptor splice site at intron 9
Length = 929
Score = 28.7 bits (61), Expect = 3.2
Identities = 13/33 (39%), Positives = 17/33 (51%)
Frame = +2
Query: 170 WLRNVRSPAITYGQPMLPTRFSKIQTMTSSSSE 268
WL VR + TYG P R +I M +S+ E
Sbjct: 59 WLDLVRKRSATYGSSGFPHRLHRIDDMVTSAGE 91
>At1g12570.1 68414.m01459 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 572
Score = 28.3 bits (60), Expect = 4.3
Identities = 22/70 (31%), Positives = 31/70 (44%)
Frame = +1
Query: 325 VLLVEAGGNPTLATEIPQPYYSNMGTSEDWAYHTEPQEGACRAYKNKGCAWPRGKVLGGS 504
VLL+E G +P I + S G + + P + R G R +VLGG
Sbjct: 70 VLLLERGDSPYNNPNITR--LSAFGAALSDLSESSPSQ---RFVSEDGVINARARVLGGG 124
Query: 505 SSINLMFYVR 534
S++N FY R
Sbjct: 125 SALNAGFYTR 134
>At3g02065.2 68416.m00170 DEAD/DEAH box helicase family protein
contains Pfam profile: PF00270 DEAD/DEAH box helicase
Length = 505
Score = 27.5 bits (58), Expect = 7.5
Identities = 11/22 (50%), Positives = 17/22 (77%)
Frame = +3
Query: 471 RLATW*GARRKQQHQFDVLRSR 536
+LA W A++K+Q FD+LRS+
Sbjct: 332 QLAIWVDAKQKKQKLFDILRSQ 353
>At3g02065.1 68416.m00171 DEAD/DEAH box helicase family protein
contains Pfam profile: PF00270 DEAD/DEAH box helicase
Length = 368
Score = 27.5 bits (58), Expect = 7.5
Identities = 11/22 (50%), Positives = 17/22 (77%)
Frame = +3
Query: 471 RLATW*GARRKQQHQFDVLRSR 536
+LA W A++K+Q FD+LRS+
Sbjct: 195 QLAIWVDAKQKKQKLFDILRSQ 216
>At4g32910.1 68417.m04683 expressed protein
Length = 716
Score = 27.1 bits (57), Expect = 9.9
Identities = 14/48 (29%), Positives = 24/48 (50%), Gaps = 3/48 (6%)
Frame = -2
Query: 150 LEDRPRYLVAVDYSSR*RW---VARPKHLEALFR*QNLNHKQVESGLI 16
+ED PRY + + W V + HL ++ L H++ E+GL+
Sbjct: 227 IEDDPRYWEVMASALSVGWLEIVVKLLHLHGSYQLDQLGHRETENGLV 274
>At2g18950.1 68415.m02212 homogentisate phytylprenyltransferase
family protein (HPT1) / tocopherol phytyltransferase
family protein (TPT1) identical to gi:17104828; contains
Pfam profile PF01040: UbiA prenyltransferase family;
identical to cDNA tocopherol polyprenyltransferase
(TPT1) GI:17104827
Length = 393
Score = 27.1 bits (57), Expect = 9.9
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = -2
Query: 471 ASLVFVGTTSPFLWFSMVSPV 409
A + VG TSPF+W ++S V
Sbjct: 327 AVAILVGATSPFIWSKVISVV 347
>At2g05120.1 68415.m00539 expressed protein
Length = 1234
Score = 27.1 bits (57), Expect = 9.9
Identities = 13/26 (50%), Positives = 15/26 (57%), Gaps = 4/26 (15%)
Frame = +1
Query: 538 NKADYDEW----AADGNEGWSFEDVL 603
N AD D+W A NEGWS E+ L
Sbjct: 1122 NAADQDDWDRHHQASTNEGWSEEETL 1147
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,997,041
Number of Sequences: 28952
Number of extensions: 264109
Number of successful extensions: 656
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 627
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 655
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1236350304
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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