BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fner10i24r (761 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC57A10.04 |mug10||sequence orphan|Schizosaccharomyces pombe|c... 29 0.55 SPBC2A9.12 |orc6|SPBC2D10.02|origin recognition complex subunit ... 28 1.7 SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce... 28 1.7 SPAC2G11.09 |||DUF221 family protein|Schizosaccharomyces pombe|c... 28 1.7 SPAC1687.20c |mis6||inner centromere protein Mis6|Schizosaccharo... 26 6.7 SPBC1703.14c |top1||DNA topoisomerase I|Schizosaccharomyces pomb... 26 6.7 SPAC23C11.16 |plo1||Polo kinase Plo1|Schizosaccharomyces pombe|c... 26 6.7 >SPAC57A10.04 |mug10||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 338 Score = 29.5 bits (63), Expect = 0.55 Identities = 15/40 (37%), Positives = 23/40 (57%) Frame = -1 Query: 239 RERNETYVQIVLISCRNQGIAVEPPTQRHLSDGHGTVPGT 120 + R E+Y Q++ C NQG+AV+ P RH+ +P T Sbjct: 202 KAREESY-QVLSALCGNQGVAVDIP--RHIKFKGSVIPAT 238 >SPBC2A9.12 |orc6|SPBC2D10.02|origin recognition complex subunit Orc6|Schizosaccharomyces pombe|chr 2|||Manual Length = 264 Score = 27.9 bits (59), Expect = 1.7 Identities = 10/19 (52%), Positives = 15/19 (78%) Frame = +1 Query: 163 VGGSTAIPWFRQLINTICT 219 +GGSTAIP+ +QL+ + T Sbjct: 117 LGGSTAIPYVKQLVKAVVT 135 >SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1610 Score = 27.9 bits (59), Expect = 1.7 Identities = 11/23 (47%), Positives = 14/23 (60%) Frame = +2 Query: 206 TRFVHMFHYVLYIKYLRHTIECE 274 T+FVH+FH LY Y T C+ Sbjct: 872 TQFVHIFHASLYTLYYVETTGCD 894 >SPAC2G11.09 |||DUF221 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 796 Score = 27.9 bits (59), Expect = 1.7 Identities = 14/42 (33%), Positives = 22/42 (52%) Frame = -1 Query: 242 YRERNETYVQIVLISCRNQGIAVEPPTQRHLSDGHGTVPGTP 117 Y ++ E Y I L +C+ GI+ PP+ +LS + TP Sbjct: 290 YVKKLEKYWSIYLSNCKKLGISTLPPS-NYLSPNRAELESTP 330 >SPAC1687.20c |mis6||inner centromere protein Mis6|Schizosaccharomyces pombe|chr 1|||Manual Length = 672 Score = 25.8 bits (54), Expect = 6.7 Identities = 19/50 (38%), Positives = 28/50 (56%), Gaps = 3/50 (6%) Frame = -2 Query: 745 NLKIYSLGSARVLMSKCLR-KKTVCVEVNHFS-QI*LFHKLLILF-VYQK 605 NL+I S+G+ L+ KCL V+HFS + L ++L LF +Y K Sbjct: 428 NLRIASMGNNHTLLEKCLLITNHATFLVSHFSWDVSLAYQLSRLFQLYYK 477 >SPBC1703.14c |top1||DNA topoisomerase I|Schizosaccharomyces pombe|chr 2|||Manual Length = 814 Score = 25.8 bits (54), Expect = 6.7 Identities = 18/61 (29%), Positives = 26/61 (42%), Gaps = 4/61 (6%) Frame = +1 Query: 61 RTNAWKLSKRQHQLLLN-G*GVPGTVPWPSDRWRWVGGSTAIPW---FRQLINTICTYVS 228 +T + K Q+ +N G GVP P P +W V + W + + IN YV Sbjct: 348 KTGSLKRRVYPEQITINIGEGVPVPEPLPGHQWAEVKHDNTVTWLATWHENINNNVKYVF 407 Query: 229 L 231 L Sbjct: 408 L 408 >SPAC23C11.16 |plo1||Polo kinase Plo1|Schizosaccharomyces pombe|chr 1|||Manual Length = 683 Score = 25.8 bits (54), Expect = 6.7 Identities = 14/37 (37%), Positives = 20/37 (54%) Frame = -2 Query: 745 NLKIYSLGSARVLMSKCLRKKTVCVEVNHFSQI*LFH 635 N+KI G A +LM RK T+C N+ + LF+ Sbjct: 176 NVKIGDFGLAALLMDDEERKMTICGTPNYIAPEILFN 212 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,982,286 Number of Sequences: 5004 Number of extensions: 59472 Number of successful extensions: 124 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 121 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 124 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 365309308 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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