Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner10i19f
(602 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 318 2e-87
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 318 2e-87
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 318 2e-87
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 318 2e-87
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 185 2e-47
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 179 2e-45
At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 102 2e-22
At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 97 8e-21
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 56 1e-08
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 56 1e-08
At5g08650.1 68418.m01029 GTP-binding protein LepA, putative 52 2e-07
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 52 3e-07
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 52 4e-07
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 48 5e-06
At4g18330.2 68417.m02719 eukaryotic translation initiation facto... 43 2e-04
At4g18330.1 68417.m02718 eukaryotic translation initiation facto... 43 2e-04
At5g13650.2 68418.m01585 elongation factor family protein contai... 42 2e-04
At5g13650.1 68418.m01584 elongation factor family protein contai... 42 2e-04
At2g18720.1 68415.m02180 eukaryotic translation initiation facto... 41 7e-04
At1g04170.1 68414.m00407 eukaryotic translation initiation facto... 40 0.001
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 39 0.002
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 37 0.012
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 37 0.012
At4g11160.1 68417.m01808 translation initiation factor IF-2, mit... 34 0.063
At3g01360.1 68416.m00057 expressed protein contains Pfam profile... 31 0.78
At1g76720.1 68414.m08929 eukaryotic translation initiation facto... 30 1.0
At1g21160.1 68414.m02646 eukaryotic translation initiation facto... 30 1.0
At1g76825.1 68414.m08940 eukaryotic translation initiation facto... 30 1.4
At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 30 1.4
At1g17220.1 68414.m02098 translation initiation factor IF-2, chl... 29 1.8
At1g26130.1 68414.m03190 haloacid dehalogenase-like hydrolase fa... 28 5.5
At5g46180.1 68418.m05680 ornithine aminotransferase, putative / ... 27 9.6
At4g20430.1 68417.m02981 subtilase family protein contains Pfam ... 27 9.6
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 318 bits (781), Expect = 2e-87
Identities = 151/180 (83%), Positives = 163/180 (90%)
Frame = +3
Query: 63 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 242
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 243 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 422
DKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 423 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 602
G FEAGISK+GQTREHALLAFTLGVKQ+I NKMD+T P YS+ R++EI KEVSSY+KK
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKK 180
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 318 bits (781), Expect = 2e-87
Identities = 151/180 (83%), Positives = 163/180 (90%)
Frame = +3
Query: 63 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 242
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 243 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 422
DKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 423 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 602
G FEAGISK+GQTREHALLAFTLGVKQ+I NKMD+T P YS+ R++EI KEVSSY+KK
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKK 180
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 318 bits (781), Expect = 2e-87
Identities = 151/180 (83%), Positives = 163/180 (90%)
Frame = +3
Query: 63 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 242
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 243 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 422
DKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 423 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 602
G FEAGISK+GQTREHALLAFTLGVKQ+I NKMD+T P YS+ R++EI KEVSSY+KK
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKK 180
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 318 bits (781), Expect = 2e-87
Identities = 151/180 (83%), Positives = 163/180 (90%)
Frame = +3
Query: 63 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 242
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 243 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 422
DKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 423 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 602
G FEAGISK+GQTREHALLAFTLGVKQ+I NKMD+T P YS+ R++EI KEVSSY+KK
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKK 180
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 185 bits (450), Expect = 2e-47
Identities = 84/175 (48%), Positives = 124/175 (70%), Gaps = 1/175 (0%)
Frame = +3
Query: 78 THINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKA 257
+ +N+ ++GHVDSGKST +G L++ G I ++ + K+EKEA+ GKGSF YAW LD+
Sbjct: 238 SQLNLAIVGHVDSGKSTLSGRLLHLLGRISQKQMHKYEKEAKLQGKGSFAYAWALDESAE 297
Query: 258 ERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEA 437
ERERGIT+ +A+ F + +++V ++D+PGH+DF+ NMI G +QAD A+L++ A G FEA
Sbjct: 298 ERERGITMTVAVAYFNSKRHHVVLLDSPGHKDFVPNMIAGATQADAAILVIDASVGAFEA 357
Query: 438 GISK-NGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIK 599
G GQTREHA + GV+Q+IV +NKMD YS+ RF+ IK+ V S+++
Sbjct: 358 GFDNLKGQTREHARVLRGFGVEQVIVAINKMDIV--GYSKERFDLIKQHVGSFLQ 410
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 179 bits (435), Expect = 2e-45
Identities = 81/176 (46%), Positives = 123/176 (69%)
Frame = +3
Query: 72 EKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKL 251
+K H+N+V IGHVD+GKST G +++ G +D R I+K+EKEA++ + S+ A+++D
Sbjct: 98 KKRHLNVVFIGHVDAGKSTIGGQILFLSGQVDDRQIQKYEKEAKDKSRESWYMAYIMDTN 157
Query: 252 KAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEF 431
+ ER +G T+++ FET TI+DAPGH+ ++ NMI+G SQAD VL+++A GEF
Sbjct: 158 EEERLKGKTVEVGRAHFETESTRFTILDAPGHKSYVPNMISGASQADIGVLVISARKGEF 217
Query: 432 EAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIK 599
E G + GQTREH LA TLGV +LIV VNKMD +S+ R++EI++++ ++K
Sbjct: 218 ETGYERGGQTREHVQLAKTLGVSKLIVVVNKMDDPTVNWSKERYDEIEQKMVPFLK 273
>At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA)
identical to SWISS-PROT:P17745 elongation factor Tu,
chloroplast precursor (EF-Tu) [Arabidopsis thaliana]
Length = 476
Score = 102 bits (245), Expect = 2e-22
Identities = 63/179 (35%), Positives = 93/179 (51%), Gaps = 1/179 (0%)
Frame = +3
Query: 60 KMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWV 239
K ++K H+NI IGHVD GK+T T L I +K+++
Sbjct: 72 KFERKKPHVNIGTIGHVDHGKTTLTAALTMALASIGSSVAKKYDE--------------- 116
Query: 240 LDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG 419
+D ER RGITI+ A ++ET + +D PGH D++KNMITG +Q D A+L+V+
Sbjct: 117 IDAAPEERARGITINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 176
Query: 420 TGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFE-EIKKEVSSY 593
G QT+EH LLA +GV ++V +NK D + E E+++ +SSY
Sbjct: 177 DGPMP-------QTKEHILLAKQVGVPDMVVFLNKEDQVDDAELLELVELEVRELLSSY 228
>At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,
putative similar to mitochondrial elongation factor Tu
[Arabidopsis thaliana] gi|1149571|emb|CAA61511
Length = 454
Score = 97.1 bits (231), Expect = 8e-21
Identities = 62/177 (35%), Positives = 90/177 (50%)
Frame = +3
Query: 69 KEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDK 248
+ K H+N+ IGHVD GK+T T + K E GK +DK
Sbjct: 63 RNKPHVNVGTIGHVDHGKTTLTAAIT---------------KVLAEEGKAKAIAFDEIDK 107
Query: 249 LKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGE 428
E++RGITI A ++ET+K + +D PGH D++KNMITG +Q D +L+V+ G
Sbjct: 108 APEEKKRGITIATAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGP 167
Query: 429 FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIK 599
QT+EH LLA +GV L+ +NK+D + P E +E+ S+ K
Sbjct: 168 MP-------QTKEHILLARQVGVPSLVCFLNKVDVVDDPELLELVEMELRELLSFYK 217
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 56.4 bits (130), Expect = 1e-08
Identities = 41/128 (32%), Positives = 61/128 (47%)
Frame = +3
Query: 87 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 266
NI + H+DSGK+T T +++ G I E E ++ G G+ +D + ERE
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRIH----EIHEVRGRD-GVGA-----KMDSMDLERE 116
Query: 267 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 446
+GITI A Y V IID PGH DF + D A+L++ + G I+
Sbjct: 117 KGITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSIT 176
Query: 447 KNGQTREH 470
+ Q R +
Sbjct: 177 VDRQMRRY 184
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 56.4 bits (130), Expect = 1e-08
Identities = 41/128 (32%), Positives = 61/128 (47%)
Frame = +3
Query: 87 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 266
NI + H+DSGK+T T +++ G I E E ++ G G+ +D + ERE
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRIH----EIHEVRGRD-GVGA-----KMDSMDLERE 116
Query: 267 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 446
+GITI A Y V IID PGH DF + D A+L++ + G I+
Sbjct: 117 KGITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSIT 176
Query: 447 KNGQTREH 470
+ Q R +
Sbjct: 177 VDRQMRRY 184
>At5g08650.1 68418.m01029 GTP-binding protein LepA, putative
Length = 681
Score = 52.4 bits (120), Expect = 2e-07
Identities = 52/174 (29%), Positives = 81/174 (46%), Gaps = 4/174 (2%)
Frame = +3
Query: 87 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 266
N +I H+D GKST L+ G + R + K F LD + ERE
Sbjct: 88 NFSIIAHIDHGKSTLADKLLQVTGTVQNRDM-----------KEQF-----LDNMDLERE 131
Query: 267 RGITIDIAL----WKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFE 434
RGITI + + +E + + + +ID PGH DF + + + A+L+V A G E
Sbjct: 132 RGITIKLQAARMRYVYEDTPFCLNLIDTPGHVDFSYEVSRSLAACEGALLVVDASQG-VE 190
Query: 435 AGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYI 596
A QT + LA + ++I +NK+D P +EP E++ +E+ I
Sbjct: 191 A------QTLANVYLALENNL-EIIPVLNKIDL---PGAEP--EKVLREIEEVI 232
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 52.0 bits (119), Expect = 3e-07
Identities = 36/113 (31%), Positives = 53/113 (46%)
Frame = +3
Query: 87 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 266
NI ++ HVD GK+T HLI GG + GK F +D L E+
Sbjct: 11 NICILAHVDHGKTTLADHLIASSGG--------GVLHPRLAGKLRF-----MDYLDEEQR 57
Query: 267 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 425
R IT+ + + Y + +ID+PGH DF + T +D A+++V A G
Sbjct: 58 RAITMKSSSISLKYKDYSLNLIDSPGHMDFCSEVSTAARLSDGALVLVDAVEG 110
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 51.6 bits (118), Expect = 4e-07
Identities = 36/117 (30%), Positives = 56/117 (47%)
Frame = +3
Query: 75 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLK 254
K + NI ++ H+D+GK+TTT ++Y G K E+ +G+ W ++
Sbjct: 94 KDYRNIGIMAHIDAGKTTTTERILYYTGRNYK---------IGEVHEGTATMDW----ME 140
Query: 255 AERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 425
E+ERGITI A K+ + IID PGH DF + D A+ + + G
Sbjct: 141 QEQERGITITSAATTTFWDKHRINIIDTPGHVDFTLEVERALRVLDGAICLFDSVAG 197
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 48.0 bits (109), Expect = 5e-06
Identities = 58/189 (30%), Positives = 80/189 (42%), Gaps = 9/189 (4%)
Frame = +3
Query: 51 D*PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKY 230
D K EK N +I H+D GKST L+ G I K G G +Y
Sbjct: 57 DLTKFPSEKIR-NFSIIAHIDHGKSTLADRLMELTGTIKK-------------GHGQPQY 102
Query: 231 AWVLDKLKAERERGITIDIALWKF---------ETSKYYVTIIDAPGHRDFIKNMITGTS 383
LDKL +RERGIT+ E S Y + +ID PGH DF + S
Sbjct: 103 ---LDKL--QRERGITVKAQTATMFYENKVEDQEASGYLLNLIDTPGHVDFSYEVSRSLS 157
Query: 384 QADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRF 563
A+L+V A G QT + LAF + ++ +NK+D P ++P
Sbjct: 158 ACQGALLVVDAAQG-------VQAQTVANFYLAFEANL-TIVPVINKIDQ---PTADP-- 204
Query: 564 EEIKKEVSS 590
E +K ++ S
Sbjct: 205 ERVKAQLKS 213
>At4g18330.2 68417.m02719 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 471
Score = 42.7 bits (96), Expect = 2e-04
Identities = 48/192 (25%), Positives = 85/192 (44%), Gaps = 14/192 (7%)
Frame = +3
Query: 57 PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDK-RTIEKFEKEAQ-EMGKGSFK- 227
P++ + INI IGHV GKST ++ G+ R + E+ ++G + K
Sbjct: 31 PEVISRQATINIGTIGHVAHGKST----IVKAISGVQTVRFKNELERNITIKLGYANAKI 86
Query: 228 YAWVLDKLK---------AERERGITIDIALWKF--ETSKYYVTIIDAPGHRDFIKNMIT 374
Y DK + +E T D+ ++ T + +V+ +D PGH + M+
Sbjct: 87 YKCEDDKCPRPMCYKSYGSGKEDNPTCDVPGFENCKMTLRRHVSCVDCPGHDILMATMLN 146
Query: 375 GTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE 554
G + D A+L++AA QT EH + +K +I+ NK+D +
Sbjct: 147 GAAIVDGALLLIAANES------CPQPQTAEHLASVDMMRLKHIIILQNKIDLINEKAAT 200
Query: 555 PRFEEIKKEVSS 590
+ E I+K +++
Sbjct: 201 EQHEAIQKFITN 212
>At4g18330.1 68417.m02718 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 284
Score = 42.7 bits (96), Expect = 2e-04
Identities = 48/192 (25%), Positives = 85/192 (44%), Gaps = 14/192 (7%)
Frame = +3
Query: 57 PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDK-RTIEKFEKEAQ-EMGKGSFK- 227
P++ + INI IGHV GKST ++ G+ R + E+ ++G + K
Sbjct: 31 PEVISRQATINIGTIGHVAHGKST----IVKAISGVQTVRFKNELERNITIKLGYANAKI 86
Query: 228 YAWVLDKLK---------AERERGITIDIALWKF--ETSKYYVTIIDAPGHRDFIKNMIT 374
Y DK + +E T D+ ++ T + +V+ +D PGH + M+
Sbjct: 87 YKCEDDKCPRPMCYKSYGSGKEDNPTCDVPGFENCKMTLRRHVSCVDCPGHDILMATMLN 146
Query: 375 GTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE 554
G + D A+L++AA QT EH + +K +I+ NK+D +
Sbjct: 147 GAAIVDGALLLIAANES------CPQPQTAEHLASVDMMRLKHIIILQNKIDLINEKAAT 200
Query: 555 PRFEEIKKEVSS 590
+ E I+K +++
Sbjct: 201 EQHEAIQKFITN 212
>At5g13650.2 68418.m01585 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 676
Score = 42.3 bits (95), Expect = 2e-04
Identities = 46/159 (28%), Positives = 66/159 (41%)
Frame = +3
Query: 87 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 266
NI ++ HVD GK+T ++ + K ++ Q M + ++D ERE
Sbjct: 85 NIAIVAHVDHGKTTLVDSMLRQA---------KVFRDNQVMQER------IMDSNDLERE 129
Query: 267 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 446
RGITI V IID PGH DF + + D +L+V + G
Sbjct: 130 RGITILSKNTSITYKNTKVNIIDTPGHSDFGGEVERVLNMVDGVLLVVDSVEGPMP---- 185
Query: 447 KNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRF 563
QTR A G ++V VNK+D P + P F
Sbjct: 186 ---QTRFVLKKALEFG-HAVVVVVNKIDR---PSARPEF 217
>At5g13650.1 68418.m01584 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 675
Score = 42.3 bits (95), Expect = 2e-04
Identities = 46/159 (28%), Positives = 66/159 (41%)
Frame = +3
Query: 87 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 266
NI ++ HVD GK+T ++ + K ++ Q M + ++D ERE
Sbjct: 84 NIAIVAHVDHGKTTLVDSMLRQA---------KVFRDNQVMQER------IMDSNDLERE 128
Query: 267 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 446
RGITI V IID PGH DF + + D +L+V + G
Sbjct: 129 RGITILSKNTSITYKNTKVNIIDTPGHSDFGGEVERVLNMVDGVLLVVDSVEGPMP---- 184
Query: 447 KNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRF 563
QTR A G ++V VNK+D P + P F
Sbjct: 185 ---QTRFVLKKALEFG-HAVVVVVNKIDR---PSARPEF 216
>At2g18720.1 68415.m02180 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative
Length = 465
Score = 40.7 bits (91), Expect = 7e-04
Identities = 44/186 (23%), Positives = 82/186 (44%), Gaps = 8/186 (4%)
Frame = +3
Query: 57 PKMGKEKTHINIVVIGHVDSGKST----TTG-HLIYKCGGIDKR-TIEKFEKEAQEMGKG 218
P++ + INI IGHV GKST +G H + +++ TI+ A+
Sbjct: 25 PEVISRQATINIGTIGHVAHGKSTIVKAVSGVHTVKFKSELERNITIKLGYANAKIYKCE 84
Query: 219 SFKYAWVLDKLKAERERGITIDIALWKFETSKY--YVTIIDAPGHRDFIKNMITGTSQAD 392
+ +E + D++ + K +V+ +D PGH + M+ G + D
Sbjct: 85 KCPRPVAYKSFGSGKEDNPSCDVSGHEKYKMKLLRHVSFVDCPGHDILMATMLNGAAIMD 144
Query: 393 CAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEI 572
A+LI+AA QT EH + +K +I+ NK+D + + + E+I
Sbjct: 145 GALLIIAANE------TCPQPQTAEHLASVDMMHLKDIIIIQNKIDLIQENEAIKQHEDI 198
Query: 573 KKEVSS 590
++ +++
Sbjct: 199 QRFITN 204
>At1g04170.1 68414.m00407 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to gb|U37354 from S. pombe. ESTs
gb|T41979, gb|N37284 and gb|N37529 come from this gene
Length = 465
Score = 40.3 bits (90), Expect = 0.001
Identities = 28/103 (27%), Positives = 49/103 (47%), Gaps = 4/103 (3%)
Frame = +3
Query: 300 FETSKY----YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTRE 467
FE SK +V+ +D PGH + M+ G + D A+L++AA QT E
Sbjct: 112 FENSKMKLLRHVSFVDCPGHDILMATMLNGAAIMDGALLLIAANE------TCPQPQTSE 165
Query: 468 HALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYI 596
H + +K +I+ NK+D + + + E I+K + + +
Sbjct: 166 HLAAVEIMQLKHIIILQNKIDLIQENVAINQHEAIQKFIMNTV 208
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 39.1 bits (87), Expect = 0.002
Identities = 30/112 (26%), Positives = 51/112 (45%), Gaps = 4/112 (3%)
Frame = +3
Query: 87 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRT---IEKFEKEAQEMGKG-SFKYAWVLDKLK 254
N+ VI HVD GKST T L+ G I + + + A E +G + K + +
Sbjct: 21 NMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTRADEAERGITIKSTGISLYYE 80
Query: 255 AERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 410
E ++ + ++Y + +ID+PGH DF + D A+++V
Sbjct: 81 MTDE---SLKSFTGARDGNEYLINLIDSPGHVDFSSEVTAALRITDGALVVV 129
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 36.7 bits (81), Expect = 0.012
Identities = 35/118 (29%), Positives = 49/118 (41%), Gaps = 5/118 (4%)
Frame = +3
Query: 87 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 266
N+ ++GH+ GK+ L+ + + K EK KY D E+E
Sbjct: 140 NVALVGHLQHGKTVFMDMLVEQTHHMSTFNA-KNEKH--------MKYT---DTRVDEQE 187
Query: 267 RGITI-----DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 425
R I+I + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 188 RNISIKAVPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 36.7 bits (81), Expect = 0.012
Identities = 35/118 (29%), Positives = 49/118 (41%), Gaps = 5/118 (4%)
Frame = +3
Query: 87 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 266
N+ ++GH+ GK+ L+ + + K EK KY D E+E
Sbjct: 140 NVALVGHLQHGKTVFMDMLVEQTHHMSTFNA-KNEKH--------MKYT---DTRVDEQE 187
Query: 267 RGITI-----DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 425
R I+I + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 188 RNISIKAVPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At4g11160.1 68417.m01808 translation initiation factor IF-2,
mitochondrial, putative similar to SP|P46198|IF2M_BOVIN
Translation initiation factor IF-2, mitochondrial
precursor (IF-2Mt) (IF-2(Mt)) {Bos taurus}
Length = 743
Score = 34.3 bits (75), Expect = 0.063
Identities = 28/90 (31%), Positives = 43/90 (47%)
Frame = +3
Query: 321 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 500
+T +D PGH F + G + D VL+VAA G QT E A + V
Sbjct: 270 ITFLDTPGHAAFSEMRARGAAVTDIVVLVVAADDGVMP-------QTLEAIAHARSANV- 321
Query: 501 QLIVGVNKMDSTEPPYSEPRFEEIKKEVSS 590
++V +NK D P + P E++K +++S
Sbjct: 322 PVVVAINKCDK---PGANP--EKVKYQLTS 346
>At3g01360.1 68416.m00057 expressed protein contains Pfam profile
PF04819: Family of unknown function (DUF716) (Plant
viral-response family)
Length = 319
Score = 30.7 bits (66), Expect = 0.78
Identities = 27/87 (31%), Positives = 38/87 (43%), Gaps = 2/87 (2%)
Frame = +3
Query: 330 IDAPGHRDFIKNMITGTSQADCAV-LIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQL 506
I GH D+ + T Q +C + L+V TG F +KNG R+ LG +
Sbjct: 226 IKCKGHGDYHRAKAIATLQFNCHLALMVVVATGLFSVIANKNGYLRQDHSKYRPLGAELE 285
Query: 507 IVGVNKMDSTEPPYSEPRFE-EIKKEV 584
+ +DS E E R E + KEV
Sbjct: 286 NLSTFTLDSDEE--DEVREESNVAKEV 310
>At1g76720.1 68414.m08929 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1201
Score = 30.3 bits (65), Expect = 1.0
Identities = 21/68 (30%), Positives = 34/68 (50%)
Frame = +3
Query: 327 IIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQL 506
+ID PGH F G+S D A+L+V + + G+ QT E +L + +
Sbjct: 705 VIDTPGHESFTNLRSRGSSLCDLAILVV-----DIKHGLEP--QTIE-SLNLLRMRNTEF 756
Query: 507 IVGVNKMD 530
I+ +NK+D
Sbjct: 757 IIALNKVD 764
>At1g21160.1 68414.m02646 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1088
Score = 30.3 bits (65), Expect = 1.0
Identities = 22/70 (31%), Positives = 33/70 (47%)
Frame = +3
Query: 321 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 500
+ +ID PGH F G++ D A+L+V + G+ QT E L VK
Sbjct: 558 ILVIDTPGHESFTNLRSRGSNLCDLAILVV-----DIMRGLEP--QTIESLNLLRRRNVK 610
Query: 501 QLIVGVNKMD 530
I+ +NK+D
Sbjct: 611 -FIIALNKVD 619
>At1g76825.1 68414.m08940 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profile PF00009: Elongation
factor Tu GTP binding domain
Length = 630
Score = 29.9 bits (64), Expect = 1.4
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +3
Query: 327 IIDAPGHRDFIKNMITGTSQADCAVLIV 410
+ID PGH F G+S D A+L+V
Sbjct: 113 VIDTPGHESFTNLRSRGSSLCDLAILVV 140
>At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to IF2
protein [Drosophila melanogaster] GI:7108770; contains
Pfam profile PF03144: Elongation factor Tu domain 2
Length = 1294
Score = 29.9 bits (64), Expect = 1.4
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +3
Query: 327 IIDAPGHRDFIKNMITGTSQADCAVLIV 410
+ID PGH F G+S D A+L+V
Sbjct: 772 VIDTPGHESFTNLRSRGSSLCDLAILVV 799
>At1g17220.1 68414.m02098 translation initiation factor IF-2,
chloroplast, putative similar to SP|P57997|IF2C_PHAVU
Translation initiation factor IF-2, chloroplast
precursor (PvIF2cp) {Phaseolus vulgaris}
Length = 1026
Score = 29.5 bits (63), Expect = 1.8
Identities = 26/99 (26%), Positives = 38/99 (38%), Gaps = 4/99 (4%)
Frame = +3
Query: 246 KLKAERERGITIDIALWKFET----SKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 413
K+ A GIT I +K +D PGH F G D A+++VA
Sbjct: 525 KVAASEAGGITQGIGAYKVSVPVDGKLQSCVFLDTPGHEAFGAMRARGARVTDIAIIVVA 584
Query: 414 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD 530
A G QT E A+ +++ +NK+D
Sbjct: 585 ADDG-------IRPQTNE-AIAHAKAAAVPIVIAINKID 615
>At1g26130.1 68414.m03190 haloacid dehalogenase-like hydrolase
family protein similar to Potential
phospholipid-transporting ATPase (EC 3.6.3.1) from Homo
sapiens [SP|Q9Y2Q0, SP|O43520], Mus musculus [SP|P98200,
SP|P70704], {Bos taurus} SP|Q29449; contains InterPro
accession IPR005834: Haloacid dehalogenase-like
hydrolase
Length = 1184
Score = 27.9 bits (59), Expect = 5.5
Identities = 15/53 (28%), Positives = 24/53 (45%)
Frame = +3
Query: 432 EAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSS 590
E+G +TR+H G++ LI+ ++D E R E K VS+
Sbjct: 629 ESGRKYEKETRDHVNEYADAGLRTLILAYRELDENEYEVFTERISEAKNSVSA 681
>At5g46180.1 68418.m05680 ornithine aminotransferase, putative /
ornithine--oxo-acid aminotransferase, putative similar
to SP|Q92413 Ornithine aminotransferase (EC 2.6.1.13)
(Ornithine--oxo-acid aminotransferase) [Aspergillus
nidulans] {Emericella nidulans}; contains Pfam profile
PF00202: aminotransferase, class III
Length = 475
Score = 27.1 bits (57), Expect = 9.6
Identities = 12/36 (33%), Positives = 21/36 (58%)
Frame = +3
Query: 486 TLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSY 593
T GV++ G+ + +S PP S R E++ E S++
Sbjct: 18 TAGVRRSYGGLPQSNSKSPPSSSQRLMELESEFSAH 53
>At4g20430.1 68417.m02981 subtilase family protein contains Pfam
profile: PF00082 subtilase family
Length = 856
Score = 27.1 bits (57), Expect = 9.6
Identities = 20/58 (34%), Positives = 27/58 (46%)
Frame = -1
Query: 503 LFDTEGESEQGMLTGLTVLRDTSFEFTGTGSYDEHSAISLRGSCDHVLDEISVSRSIN 330
+FDT E L G+ F +TGT ++ IS GS D L I+VS+ N
Sbjct: 714 IFDTSFEDYMSFLCGINGSAPVVFNYTGTNCLRNNATIS--GS-DLNLPSITVSKLNN 768
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,915,405
Number of Sequences: 28952
Number of extensions: 257559
Number of successful extensions: 808
Number of sequences better than 10.0: 33
Number of HSP's better than 10.0 without gapping: 768
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 793
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1197101088
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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