Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner10i16f
(642 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g06000.1 68418.m00665 eukaryotic translation initiation facto... 80 1e-15
At3g11400.1 68416.m01390 eukaryotic translation initiation facto... 72 3e-13
At1g43560.1 68414.m05000 thioredoxin family protein contains Pfa... 29 2.6
At3g03630.1 68416.m00366 cysteine synthase, chloroplast, putativ... 29 3.5
At2g30960.1 68415.m03776 expressed protein 29 3.5
At1g52650.1 68414.m05945 F-box family protein contains F-box dom... 28 6.1
At5g57170.1 68418.m07141 macrophage migration inhibitory factor ... 27 8.0
At2g36350.1 68415.m04461 protein kinase, putative similar to pro... 27 8.0
At1g76760.1 68414.m08933 thioredoxin family protein similar to t... 27 8.0
At1g67370.1 68414.m07669 meiotic asynaptic mutant 1 (ASY1) ident... 27 8.0
At1g57820.2 68414.m06561 zinc finger (C3HC4-type RING finger) fa... 27 8.0
At1g57820.1 68414.m06560 zinc finger (C3HC4-type RING finger) fa... 27 8.0
>At5g06000.1 68418.m00665 eukaryotic translation initiation factor
3G, putative / eIF3g, putative similar to eukaryotic
translation initiation factor 3g [Arabidopsis thaliana]
GI:12407751; contains InterPro entry IPR000504:
RNA-binding region RNP-1 (RNA recognition motif) (RRM)
Length = 276
Score = 80.2 bits (189), Expect = 1e-15
Identities = 43/119 (36%), Positives = 68/119 (57%), Gaps = 5/119 (4%)
Frame = +1
Query: 205 ENGLKIVTEYKYDNDNKKVKIVRTYKIEKRVVSKSIAKRKTWSKFGDSASDKPGPNPATT 384
+NG+K V EYK++ ++KKVKI T +++KR ++K +R++W+KFGD+A ++ + T
Sbjct: 6 QNGVKKVIEYKFNEEDKKVKITTTTRVQKRALTKQAVERRSWNKFGDAAHEE-SSSYLTM 64
Query: 385 NVAEDVFMQFIT---SKEESQRPDDGELDGLKPPSSNVIFKCRTCQ--GDHLTLYCPFK 546
ED+ ++ I S E + L P + V+ CR CQ GDH T CP K
Sbjct: 65 RSTEDIILERIRAPGSNAEQSTVSGDSMSQLGKPGA-VLMVCRLCQKKGDHWTSRCPQK 122
>At3g11400.1 68416.m01390 eukaryotic translation initiation factor
3G / eIF3g nearly identical to eukaryotic translation
initiation factor 3g [Arabidopsis thaliana] GI:12407751
Length = 294
Score = 72.1 bits (169), Expect = 3e-13
Identities = 52/150 (34%), Positives = 80/150 (53%), Gaps = 12/150 (8%)
Frame = +1
Query: 133 EFQASWADEVEIDQGVLPPPSEVV---ENGLKIVTEYKYDNDNKKVKI---VRTYKIEKR 294
+F+ DE E D L PP +V+ ENGLK EYK++++ KVKI R K+
Sbjct: 11 KFRWGEMDEDE-DLDFLLPPKQVIGPDENGLKTTIEYKFNDEENKVKITTRTRVRKLASA 69
Query: 295 VVSKSIAKRKTWSKFGDSASDKPGPNPATTNVAEDVFMQFI----TSKEESQRPDDGELD 462
++K +R+ W KFGD+A+++ G + T E++ ++ T +ES+ DG L
Sbjct: 70 RLNKRAMERRNWPKFGDAANEEAGSH-LTMVSTEEILLERPRAPGTKADESKATGDG-LS 127
Query: 463 GLKPPSSNVIFKCRTC--QGDHLTLYCPFK 546
L + V+ CR C +GDH T CP+K
Sbjct: 128 QLGKGGA-VLMVCRICHKKGDHWTSKCPYK 156
>At1g43560.1 68414.m05000 thioredoxin family protein contains Pfam
profile: PF00085 Thioredoxin; similar to thioredoxin
GI:142153 from [Synechococcus PCC6301]
Length = 167
Score = 29.1 bits (62), Expect = 2.6
Identities = 20/75 (26%), Positives = 32/75 (42%)
Frame = +1
Query: 115 DMPVAEEFQASWADEVEIDQGVLPPPSEVVENGLKIVTEYKYDNDNKKVKIVRTYKIEKR 294
D PV +F A+W ++ ++P +EV E I+ K D + K + Y+IE
Sbjct: 76 DKPVLVDFYATWCGPCQL---MVPILNEVSETLKDIIAVVKIDTE-KYPSLANKYQIEAL 131
Query: 295 VVSKSIAKRKTWSKF 339
K W +F
Sbjct: 132 PTFILFKDGKLWDRF 146
>At3g03630.1 68416.m00366 cysteine synthase, chloroplast, putative /
O-acetylserine (thiol)-lyase, putative / O-acetylserine
sulfhydrylase, putative identical to SP|O22682 Probable
cysteine synthase, chloroplast precursor {Arabidopsis
thaliana}, similar to SP|P31300 Cysteine synthase,
chloroplast precursor {Capsicum annuum}
Length = 404
Score = 28.7 bits (61), Expect = 3.5
Identities = 17/56 (30%), Positives = 24/56 (42%)
Frame = +1
Query: 232 YKYDNDNKKVKIVRTYKIEKRVVSKSIAKRKTWSKFGDSASDKPGPNPATTNVAED 399
+ + +D+ VRT + VV K T +K KP P P T VAE+
Sbjct: 34 FSFHHDSSSSLAVRT-PVSSFVVGAISGKSSTGTKSKSKTKRKPPPPPPVTTVAEE 88
>At2g30960.1 68415.m03776 expressed protein
Length = 260
Score = 28.7 bits (61), Expect = 3.5
Identities = 31/106 (29%), Positives = 53/106 (50%), Gaps = 7/106 (6%)
Frame = +1
Query: 178 VLPPPSEVVENG--LKIVTEYKYDNDNKKVKIVRTYKIEKRVVSKSI----AKR-KTWSK 336
++PP S V EN +K V + K +I ++ IE + + SI AK KT +K
Sbjct: 160 IVPPESVVTENNTPVKDVVPVYWMKATKSKEITKSMAIETQFENFSINRGEAKTLKTQTK 219
Query: 337 FGDSASDKPGPNPATTNVAEDVFMQFITSKEESQRPDDGELDGLKP 474
+ ++P P A+++ M ++ KE+++R DG G+KP
Sbjct: 220 W--RYRNRPMPRGASSST-----MVWV-KKEQNERAGDGIASGVKP 257
>At1g52650.1 68414.m05945 F-box family protein contains F-box domain
Pfam:PF00646
Length = 465
Score = 27.9 bits (59), Expect = 6.1
Identities = 12/31 (38%), Positives = 20/31 (64%)
Frame = -3
Query: 220 FLVHFQPLQKVVEVHLGQSRPHQPRKPEILQ 128
FL HF P + +++H+ ++ P Q R PE+ Q
Sbjct: 404 FLEHF-PCLREIKIHMEENGPTQLRVPEVSQ 433
>At5g57170.1 68418.m07141 macrophage migration inhibitory factor
family protein / MIF family protein contains Pfam
profile: PF01187 Macrophage migration inhibitory
factor(MIF)
Length = 115
Score = 27.5 bits (58), Expect = 8.0
Identities = 10/35 (28%), Positives = 23/35 (65%)
Frame = -2
Query: 380 VAGLGPGLSLAESPNLLQVLRLAILFETTRFSILY 276
+ GLGPG++ S + ++L++ + +++RF I +
Sbjct: 66 IGGLGPGVNGKLSETISEILQIKLSIDSSRFYIKF 100
>At2g36350.1 68415.m04461 protein kinase, putative similar to
protein kinase KIPK (KCBP-interacting protein kinase)
[Arabidopsis thaliana] gi|7716430|gb|AAF68383
Length = 949
Score = 27.5 bits (58), Expect = 8.0
Identities = 15/54 (27%), Positives = 28/54 (51%)
Frame = +1
Query: 157 EVEIDQGVLPPPSEVVENGLKIVTEYKYDNDNKKVKIVRTYKIEKRVVSKSIAK 318
E+EID+ PP V+E+ L + ++ + V T +++K +VS + K
Sbjct: 266 EIEIDENHTSPPHMVIEDQLVEIDKHVTSLPSCSGSKVDTEELDKSIVSSARVK 319
>At1g76760.1 68414.m08933 thioredoxin family protein similar to
thioredoxin CH2, M-type, chloroplast precursor GB:P23400
SP|P23400 [Chlamydomonas reinhardtii]; contains Pfam
profile: PF00085 Thioredoxin
Length = 172
Score = 27.5 bits (58), Expect = 8.0
Identities = 17/58 (29%), Positives = 29/58 (50%)
Frame = +1
Query: 115 DMPVAEEFQASWADEVEIDQGVLPPPSEVVENGLKIVTEYKYDNDNKKVKIVRTYKIE 288
D PV ++ A+W + +L SE +++ +++V K D + K I YKIE
Sbjct: 81 DKPVLVDYYATWCGPCQFMVPILNEVSETLKDKIQVV---KIDTE-KYPSIANKYKIE 134
>At1g67370.1 68414.m07669 meiotic asynaptic mutant 1 (ASY1)
identical to meiotic asynaptic mutant 1 [Arabidopsis
thaliana] GI:7939627; contains Pfam profiles PF02301:
DNA-binding HORMA domain, PF04433: SWIRM domain
Length = 596
Score = 27.5 bits (58), Expect = 8.0
Identities = 14/40 (35%), Positives = 22/40 (55%)
Frame = +1
Query: 343 DSASDKPGPNPATTNVAEDVFMQFITSKEESQRPDDGELD 462
DS D P+ + + +++ QFI + E Q DDGE+D
Sbjct: 252 DSVHDDQ-PSDSDSEISQTQENQFIVAPVEKQDDDDGEVD 290
>At1g57820.2 68414.m06561 zinc finger (C3HC4-type RING finger)
family protein low similarity to nuclear protein np95
[Mus musculus] GI:4220590; contains Pfam profiles
PF02182: YDG/SRA domain, PF00097: Zinc finger, C3HC4
type (RING finger), PF00628: PHD-finger
Length = 642
Score = 27.5 bits (58), Expect = 8.0
Identities = 19/94 (20%), Positives = 43/94 (45%)
Frame = +1
Query: 205 ENGLKIVTEYKYDNDNKKVKIVRTYKIEKRVVSKSIAKRKTWSKFGDSASDKPGPNPATT 384
E G++ Y+ + +KV + ++K+ + + + + W+ + +P PN
Sbjct: 386 EEGVRYDGVYRIEKCWRKVGVQGSFKVCRYLFVRCDNEPAPWTSDENGDRPRPIPNIPEL 445
Query: 385 NVAEDVFMQFITSKEESQRPDDGELDGLKPPSSN 486
N+A D+F + T + +G +KPP ++
Sbjct: 446 NMATDLFERKETPSWDFDE-GEGCWKWMKPPPAS 478
>At1g57820.1 68414.m06560 zinc finger (C3HC4-type RING finger)
family protein low similarity to nuclear protein np95
[Mus musculus] GI:4220590; contains Pfam profiles
PF02182: YDG/SRA domain, PF00097: Zinc finger, C3HC4
type (RING finger), PF00628: PHD-finger
Length = 645
Score = 27.5 bits (58), Expect = 8.0
Identities = 19/94 (20%), Positives = 43/94 (45%)
Frame = +1
Query: 205 ENGLKIVTEYKYDNDNKKVKIVRTYKIEKRVVSKSIAKRKTWSKFGDSASDKPGPNPATT 384
E G++ Y+ + +KV + ++K+ + + + + W+ + +P PN
Sbjct: 389 EEGVRYDGVYRIEKCWRKVGVQGSFKVCRYLFVRCDNEPAPWTSDENGDRPRPIPNIPEL 448
Query: 385 NVAEDVFMQFITSKEESQRPDDGELDGLKPPSSN 486
N+A D+F + T + +G +KPP ++
Sbjct: 449 NMATDLFERKETPSWDFDE-GEGCWKWMKPPPAS 481
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,449,570
Number of Sequences: 28952
Number of extensions: 239347
Number of successful extensions: 728
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 713
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 724
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1324661040
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -