Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner10h14f
(618 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g21230.1 68416.m02683 4-coumarate--CoA ligase, putative / 4-c... 39 0.003
At3g48990.1 68416.m05351 AMP-dependent synthetase and ligase fam... 37 0.009
At3g21240.1 68416.m02684 4-coumarate--CoA ligase 2 / 4-coumaroyl... 36 0.028
At1g62940.1 68414.m07107 4-coumarate--CoA ligase family protein ... 35 0.050
At4g05160.1 68417.m00775 4-coumarate--CoA ligase, putative / 4-c... 33 0.15
At5g36880.1 68418.m04418 acetyl-CoA synthetase, putative / aceta... 31 0.46
At1g55180.1 68414.m06303 phospholipase D, putative (PLDEPSILON) ... 31 0.81
At1g76290.1 68414.m08860 AMP-dependent synthetase and ligase fam... 30 1.1
At1g51680.2 68414.m05823 4-coumarate--CoA ligase 1 / 4-coumaroyl... 29 1.9
At1g51680.1 68414.m05822 4-coumarate--CoA ligase 1 / 4-coumaroyl... 29 1.9
At1g20560.1 68414.m02563 AMP-dependent synthetase and ligase fam... 29 1.9
At1g20500.1 68414.m02554 4-coumarate--CoA ligase family / 4-coum... 29 2.5
At1g20490.1 68414.m02553 AMP-dependent synthetase and ligase fam... 29 2.5
At5g38120.1 68418.m04592 4-coumarate--CoA ligase family protein ... 27 7.5
At4g19010.1 68417.m02802 4-coumarate--CoA ligase family protein ... 27 7.5
At1g18500.1 68414.m02309 2-isopropylmalate synthase, putative st... 27 7.5
At4g38710.1 68417.m05483 glycine-rich protein cylicin II - bovin... 27 10.0
At3g12080.1 68416.m01504 GTP-binding family protein contains Pfa... 27 10.0
At1g49740.1 68414.m05578 expressed protein similar to MAP3K-like... 27 10.0
At1g11710.1 68414.m01344 pentatricopeptide (PPR) repeat-containi... 27 10.0
>At3g21230.1 68416.m02683 4-coumarate--CoA ligase, putative /
4-coumaroyl-CoA synthase, putative (4CL) similar to 4CL2
[gi:12229665] and 4CL1 [gi:12229649] from [Arabidopsis
thaliana], 4CL1 [gi:12229631] from Nicotiana tabacum
Length = 570
Score = 38.7 bits (86), Expect = 0.003
Identities = 19/59 (32%), Positives = 33/59 (55%)
Frame = +3
Query: 429 RTWTFGQIASKSNQVARTMQEHLQLKRGDVVCVFLPNSGEYIWTWLGIAKVGXVSALIN 605
R T+ + + ++A + L ++ GDVV + LPNS E+ ++L +A +G VS N
Sbjct: 72 RILTYADVQTNMRRIAAGIHR-LGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTAN 129
>At3g48990.1 68416.m05351 AMP-dependent synthetase and ligase family
protein similar to peroxisomal-coenzyme A synthetase
(FAT2) [gi:586339] from Saccharomyces cerevisiae;
contains Pfam AMP-binding enzyme domain PF00501;
identical to cDNA; identical to cDNA adenosine
monophosphate binding protein 3 AMPBP3
(AMPBP3)GI:20799714
Length = 514
Score = 37.1 bits (82), Expect = 0.009
Identities = 21/84 (25%), Positives = 39/84 (46%)
Frame = +3
Query: 357 TVAELFTRRAKRNPDAPCFIVVGDRTWTFGQIASKSNQVARTMQEHLQLKRGDVVCVFLP 536
T++ L AK+ PD V G T ++ + A + +K GDVV + P
Sbjct: 5 TLSGLLENVAKKFPDRRALSVSGKFNLTHARLHDLIERAASRLVSDAGIKPGDVVALTFP 64
Query: 537 NSGEYIWTWLGIAKVGXVSALINS 608
N+ E++ +L + + +A +N+
Sbjct: 65 NTVEFVIMFLAVIRARATAAPLNA 88
>At3g21240.1 68416.m02684 4-coumarate--CoA ligase 2 /
4-coumaroyl-CoA synthase 2 (4CL2) identical to SP|Q9S725
4-coumarate--CoA ligase 2 (EC 6.2.1.12) (4CL 2)
(4-coumaroyl-CoA synthase 2) {Arabidopsis thaliana}
Length = 556
Score = 35.5 bits (78), Expect = 0.028
Identities = 21/69 (30%), Positives = 38/69 (55%), Gaps = 2/69 (2%)
Frame = +3
Query: 405 PCFI--VVGDRTWTFGQIASKSNQVARTMQEHLQLKRGDVVCVFLPNSGEYIWTWLGIAK 578
PC I G+ +T+ + S ++A + +L +K+ DVV + LPNS E + T+L +
Sbjct: 53 PCLINGPTGE-VYTYADVHVTSRKLAAGLH-NLGVKQHDVVMILLPNSPEVVLTFLAASF 110
Query: 579 VGXVSALIN 605
+G ++ N
Sbjct: 111 IGAITTSAN 119
>At1g62940.1 68414.m07107 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to
gi:112801 from Petroselinum crispum, GB:AAD40664 from
[Solanum tuberosum] (J. Biol. Chem. 266 (13), 8551-8559
(1991)); contains Pfam AMP-binding enzyme domain PF00501
Length = 542
Score = 34.7 bits (76), Expect = 0.050
Identities = 22/79 (27%), Positives = 38/79 (48%), Gaps = 1/79 (1%)
Frame = +3
Query: 357 TVAELFTRRAKRNPDAPCFI-VVGDRTWTFGQIASKSNQVARTMQEHLQLKRGDVVCVFL 533
T+ E + + + F+ V + T+G + + ++A+ + L L++G V+ V L
Sbjct: 27 TLPEFVLQGVEEYTENVAFVEAVTGKAVTYGDVVRDTKRLAKALTS-LGLRKGQVMVVVL 85
Query: 534 PNSGEYIWTWLGIAKVGXV 590
PN EY LGI G V
Sbjct: 86 PNVAEYGIIALGIMSAGGV 104
>At4g05160.1 68417.m00775 4-coumarate--CoA ligase, putative /
4-coumaroyl-CoA synthase, putative similar to 4CL2
[gi:12229665] from Arabidopsis thaliana, 4CL1
[gi:12229631] from Nicotiana tabacum; contains Pfam
AMP-binding enzyme domain PF00501; acyl-activating
enzyme superfamily; identical to cDNA 4-coumarate-CoA
ligase-like protein (At4g05160) GI:29893226
Length = 544
Score = 33.1 bits (72), Expect = 0.15
Identities = 17/56 (30%), Positives = 29/56 (51%)
Frame = +3
Query: 438 TFGQIASKSNQVARTMQEHLQLKRGDVVCVFLPNSGEYIWTWLGIAKVGXVSALIN 605
TF Q+ S ++A L +++ DVV +F PNS ++ +L + +G V N
Sbjct: 56 TFSQLKSAVARLAHGFHR-LGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTAN 110
>At5g36880.1 68418.m04418 acetyl-CoA synthetase, putative /
acetate-CoA ligase, putative similar to SP|P27550
(Escherichia coli) and gi:8439651 (Homo sapiens);
contains Pfam AMP-binding enzyme domain PF00501
Length = 693
Score = 31.5 bits (68), Expect = 0.46
Identities = 15/59 (25%), Positives = 34/59 (57%)
Frame = +3
Query: 426 DRTWTFGQIASKSNQVARTMQEHLQLKRGDVVCVFLPNSGEYIWTWLGIAKVGXVSALI 602
D + T+ ++ + Q+A ++++ +K+GD V ++LP E L A++G V +++
Sbjct: 145 DASLTYSELLQRVCQLANYLKDN-GVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVV 202
>At1g55180.1 68414.m06303 phospholipase D, putative (PLDEPSILON)
identical to SP|Q9C888 Phospholipase D epsilon (EC
3.1.4.4) (AtPLDepsilon) (PLD epsilon) (PLDalpha3)
{Arabidopsis thaliana}; similar to GI:6573119 from
[Lycopersicon esculentum] (Plant Physiol. 122 (1), 292
(2000))
Length = 762
Score = 30.7 bits (66), Expect = 0.81
Identities = 12/31 (38%), Positives = 17/31 (54%)
Frame = -3
Query: 451 ICPKVHVRSPTTIKHGASGFRLARRVNNSAT 359
+CP++H + PT H L RV NS+T
Sbjct: 291 LCPRLHKKLPTAFAHHQKTITLDTRVTNSST 321
>At1g76290.1 68414.m08860 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501
Length = 546
Score = 30.3 bits (65), Expect = 1.1
Identities = 18/65 (27%), Positives = 30/65 (46%)
Frame = +3
Query: 414 IVVGDRTWTFGQIASKSNQVARTMQEHLQLKRGDVVCVFLPNSGEYIWTWLGIAKVGXVS 593
+V GD +T+ Q + ++A + + L L R DVV PN + G G V
Sbjct: 33 VVYGDIQYTWHQTRDRCVRLASALSD-LGLSRHDVVAALAPNVPALCELYFGAPMAGAVL 91
Query: 594 ALINS 608
++N+
Sbjct: 92 CVLNT 96
>At1g51680.2 68414.m05823 4-coumarate--CoA ligase 1 /
4-coumaroyl-CoA synthase 1 (4CL1) identical to SP|Q42524
4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1)
(4-coumaroyl-CoA synthase 1) {Arabidopsis thaliana}
Length = 490
Score = 29.5 bits (63), Expect = 1.9
Identities = 18/68 (26%), Positives = 32/68 (47%), Gaps = 1/68 (1%)
Frame = +3
Query: 405 PCFIV-VGDRTWTFGQIASKSNQVARTMQEHLQLKRGDVVCVFLPNSGEYIWTWLGIAKV 581
PC I +T+ + S Q+A + L + + DVV + LPN E++ ++L +
Sbjct: 55 PCLINGPTGHVYTYSDVHVISRQIAANFHK-LGVNQNDVVMLLLPNCPEFVLSFLAASFR 113
Query: 582 GXVSALIN 605
G + N
Sbjct: 114 GATATAAN 121
>At1g51680.1 68414.m05822 4-coumarate--CoA ligase 1 /
4-coumaroyl-CoA synthase 1 (4CL1) identical to SP|Q42524
4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1)
(4-coumaroyl-CoA synthase 1) {Arabidopsis thaliana}
Length = 561
Score = 29.5 bits (63), Expect = 1.9
Identities = 18/68 (26%), Positives = 32/68 (47%), Gaps = 1/68 (1%)
Frame = +3
Query: 405 PCFIV-VGDRTWTFGQIASKSNQVARTMQEHLQLKRGDVVCVFLPNSGEYIWTWLGIAKV 581
PC I +T+ + S Q+A + L + + DVV + LPN E++ ++L +
Sbjct: 55 PCLINGPTGHVYTYSDVHVISRQIAANFHK-LGVNQNDVVMLLLPNCPEFVLSFLAASFR 113
Query: 582 GXVSALIN 605
G + N
Sbjct: 114 GATATAAN 121
>At1g20560.1 68414.m02563 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501; identical to adenosine monophosphate binding
protein 1 AMPBP1 (AMPBP1) GI:20799710
Length = 556
Score = 29.5 bits (63), Expect = 1.9
Identities = 17/64 (26%), Positives = 29/64 (45%)
Frame = +3
Query: 414 IVVGDRTWTFGQIASKSNQVARTMQEHLQLKRGDVVCVFLPNSGEYIWTWLGIAKVGXVS 593
IV G +T+ Q + ++A + + L + GDVV V PN + G+ G +
Sbjct: 35 IVYGSVKYTWRQTRDRCVRIASALSQ-LGISTGDVVSVLAPNVPAMVELHFGVPMAGALL 93
Query: 594 ALIN 605
+N
Sbjct: 94 CTLN 97
>At1g20500.1 68414.m02554 4-coumarate--CoA ligase family /
4-coumaroyl-CoA synthase family similar to SP|Q42524 and
SP|Q9S725; contains Pfam AMP-binding enzyme domain
PF00501
Length = 412
Score = 29.1 bits (62), Expect = 2.5
Identities = 16/57 (28%), Positives = 27/57 (47%)
Frame = +3
Query: 438 TFGQIASKSNQVARTMQEHLQLKRGDVVCVFLPNSGEYIWTWLGIAKVGXVSALINS 608
TF + ++VA + + ++RGDVV + PNS L + +G V N+
Sbjct: 65 TFSDLWRAVDRVADCLYHEVGIRRGDVVLILSPNSIFIPVVCLSVMSLGAVFTTANT 121
>At1g20490.1 68414.m02553 AMP-dependent synthetase and ligase family
protein similar to SP|Q42524 and SP|Q9S725; contains
Pfam AMP-binding enzyme domain PF00501
Length = 447
Score = 29.1 bits (62), Expect = 2.5
Identities = 16/57 (28%), Positives = 27/57 (47%)
Frame = +3
Query: 438 TFGQIASKSNQVARTMQEHLQLKRGDVVCVFLPNSGEYIWTWLGIAKVGXVSALINS 608
TF + ++VA + + ++RGDVV + PNS L + +G V N+
Sbjct: 65 TFSDLWRVVDRVADCLYHEVGIRRGDVVLILSPNSIYIPVVCLSVMSLGAVVTTANT 121
>At5g38120.1 68418.m04592 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to 4CL2,
Arabidopsis thaliana [gi:12229665], 4CL1, Nicotiana
tabacum [gi:12229631]; contains Pfam AMP-binding enzyme
domain PF00501
Length = 550
Score = 27.5 bits (58), Expect = 7.5
Identities = 16/60 (26%), Positives = 27/60 (45%)
Frame = +3
Query: 426 DRTWTFGQIASKSNQVARTMQEHLQLKRGDVVCVFLPNSGEYIWTWLGIAKVGXVSALIN 605
D +F + ++VA + + ++RGDVV V PN+ L + +G V N
Sbjct: 62 DHRISFSDLWMAVDRVADCLLHDVGIRRGDVVLVLSPNTISIPIVCLSVMSLGAVLTTAN 121
>At4g19010.1 68417.m02802 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to 4CL
from Pinus taeda, gi:515503, gi:1143308; contains Pfam
AMP-binding enzyme domain PF00501
Length = 566
Score = 27.5 bits (58), Expect = 7.5
Identities = 12/37 (32%), Positives = 22/37 (59%)
Frame = +3
Query: 495 LQLKRGDVVCVFLPNSGEYIWTWLGIAKVGXVSALIN 605
L +++GDVV + LPNS + +L + +G + +N
Sbjct: 93 LGVRQGDVVSLVLPNSVYFPMIFLSLISLGAIVTTMN 129
>At1g18500.1 68414.m02309 2-isopropylmalate synthase, putative
strong similarity to 2-isopropylmalate synthase (IMS1)
[Arabidopsis thaliana] GI:12330687; contains Pfam
profile PF00682: HMGL-like
Length = 631
Score = 27.5 bits (58), Expect = 7.5
Identities = 16/52 (30%), Positives = 23/52 (44%)
Frame = -2
Query: 386 STSREQFRYSCILTSPSLRPYHAIRVAP*EFEVSWKSDENNVELLSVTIHTG 231
S + R SC ++ PS P H R E+ + SD N V + T+ G
Sbjct: 48 SLRSQTLRLSCSISDPSPLPPHTPRRPRPEYIPNRISDPNYVRVFDTTLRDG 99
>At4g38710.1 68417.m05483 glycine-rich protein cylicin II - bovine,
PIR2:I46014
Length = 452
Score = 27.1 bits (57), Expect = 10.0
Identities = 11/31 (35%), Positives = 21/31 (67%)
Frame = -2
Query: 611 IAVDQC*NASDFSDAKPRPDVLAAVGQEYAD 519
+ V++ A+ F +A+PR +VLA GQ++ +
Sbjct: 304 VIVEKPKGANPFGNARPREEVLAEKGQDWKE 334
>At3g12080.1 68416.m01504 GTP-binding family protein contains Pfam
domain, PF01926: GTPase of unknown function
Length = 663
Score = 27.1 bits (57), Expect = 10.0
Identities = 15/36 (41%), Positives = 19/36 (52%)
Frame = -3
Query: 607 LLISAETXPTLAMPSHVQMYSPLLGRNTQTTSPRLS 500
LL S T MPS ++YSPL + T+SP S
Sbjct: 4 LLDSLTTRGFFTMPSISRIYSPLASSTSLTSSPSSS 39
>At1g49740.1 68414.m05578 expressed protein similar to MAP3K-like
protein kinase GB:CAB16796 GI:4006878 from [Arabidopsis
thaliana]
Length = 359
Score = 27.1 bits (57), Expect = 10.0
Identities = 24/92 (26%), Positives = 35/92 (38%)
Frame = -2
Query: 506 FELQVLLHSSRHLVRFRRNLSKGPRSISNNNKTWRIGIPFSTSREQFRYSCILTSPSLRP 327
F L LL S L+ L +G I+N+N + + FR C T P + P
Sbjct: 8 FLLIALLLQSSFLLEISSALKEGKTCITNSNCDAGLHCETCIANTDFRPRCSRTQP-INP 66
Query: 326 YHAIRVAP*EFEVSWKSDENNVELLSVTIHTG 231
+ P + SW + N+ L TG
Sbjct: 67 ITKAKGLPFN-KYSWLTTHNSFARLGEVSRTG 97
>At1g11710.1 68414.m01344 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 657
Score = 27.1 bits (57), Expect = 10.0
Identities = 15/34 (44%), Positives = 20/34 (58%), Gaps = 3/34 (8%)
Frame = +2
Query: 227 SALCVSLQKA---ALHYFRHSSTRPQIPMALREW 319
SALC++L++ LH F S T P I LRE+
Sbjct: 34 SALCLNLRQRRWNTLHQFSSSLTNPLISRVLREF 67
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,577,005
Number of Sequences: 28952
Number of extensions: 254320
Number of successful extensions: 768
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 742
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 767
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1246162608
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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