SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fner10h13r
         (621 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|...    27   1.7  
SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc...    27   2.2  
SPAC13A11.01c |rga8|SPAC2F7.18c|GTPase activating protein Rga8 |...    26   3.8  
SPAC3C7.12 |tip1|noc1|CLIP170 family protein Tip1|Schizosaccharo...    26   3.8  
SPBC609.01 |||ribonuclease II |Schizosaccharomyces pombe|chr 2||...    26   5.1  
SPAC27D7.11c |||But2 family protein|Schizosaccharomyces pombe|ch...    25   6.7  
SPAC821.11 |pro1||gamma-glutamyl phosphate reductase Pro1 |Schiz...    25   6.7  
SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase Vas1|Schizos...    25   8.8  

>SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|chr
            3|||Manual
          Length = 1184

 Score = 27.5 bits (58), Expect = 1.7
 Identities = 19/83 (22%), Positives = 38/83 (45%), Gaps = 1/83 (1%)
 Frame = -3

Query: 367  GYETDNGISAQSSGSLKKVDNIDVLAIQGQYEYS-APDGTPVKFTYTADENGYQPQSELL 191
            G+  +   +A S+  +    +++V A+        A +G+ V  T   D N     S + 
Sbjct: 1089 GHSRNGSHAAHSNNVIGTQPHVNVSAVPNTGNLKDALEGSAVSKTDDVD-NVVSGHSNVN 1147

Query: 190  PVAPPMPEAIRRAIDYILAHPPK 122
             V+   P  + R+ DY+++H P+
Sbjct: 1148 GVSKSRPNIVERSEDYVISHLPE 1170



 Score = 25.8 bits (54), Expect = 5.1
 Identities = 13/36 (36%), Positives = 20/36 (55%), Gaps = 1/36 (2%)
 Frame = -3

Query: 274  EYSAPDGTPVKFTYTADENGYQPQ-SELLPVAPPMP 170
            +Y   +   V+ T T DE+G+Q Q +    VA P+P
Sbjct: 955  DYEGTEDNIVRQTTTVDEDGHQEQTTSTTKVAHPIP 990


>SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1576

 Score = 27.1 bits (57), Expect = 2.2
 Identities = 32/127 (25%), Positives = 53/127 (41%), Gaps = 10/127 (7%)
 Frame = -3

Query: 391 VNPDGFSFGYETDNGISAQSSGSLK--KVDNIDVLAIQGQYEYSA----PDGTPVKFTY- 233
           ++ D  S     +  +  + S SL   K+ ++D+   +  Y  SA    P+ + + F Y 
Sbjct: 634 ISSDSESLSQSLNMAVDFRQSSSLPADKLSSLDI-HFEDDYNSSADEEDPEASDISFQYS 692

Query: 232 -TADENGYQPQSELLPVAPPMP--EAIRRAIDYILAHPPKTETVKKL*IQKIQSTTTPAK 62
            T+D+ G   +   +  A  +   E    A  YI     + E  +KL +Q I     P  
Sbjct: 693 NTSDKEGSSDKDSSIEEASSVKTQENGLNATLYI-----QMEYCEKLSLQDIIRDKIPVD 747

Query: 61  EKWTLFR 41
           E W LFR
Sbjct: 748 EMWRLFR 754


>SPAC13A11.01c |rga8|SPAC2F7.18c|GTPase activating protein Rga8
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 777

 Score = 26.2 bits (55), Expect = 3.8
 Identities = 30/100 (30%), Positives = 48/100 (48%), Gaps = 13/100 (13%)
 Frame = -1

Query: 384 LTDSALGMKLTMESALNHLDP*RKLIISMFSPSKV----NMNTLRLTEPL-*SSRIQLTR 220
           L +S L     +  ALNH     K ++S ++PS V     +  L L + +  SS  +L R
Sbjct: 496 LVNSPLSSVHQLREALNHSSSVTKEVLSQYTPSVVIGVLKLYFLELPDSIVPSSAFELIR 555

Query: 219 T-------DTNHRVSYFQ-LLLQCLKPSVALLTTFSLTHL 124
           +       DT +R+   Q LL Q  + ++A L+   +THL
Sbjct: 556 SIYMNHSNDTPYRLRLLQNLLSQLRRVNLATLSAI-ITHL 594


>SPAC3C7.12 |tip1|noc1|CLIP170 family protein
           Tip1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 461

 Score = 26.2 bits (55), Expect = 3.8
 Identities = 17/64 (26%), Positives = 32/64 (50%)
 Frame = -1

Query: 351 MESALNHLDP*RKLIISMFSPSKVNMNTLRLTEPL*SSRIQLTRTDTNHRVSYFQLLLQC 172
           +E  L  +D  + L+ + F+  +  ++ LR    L   RIQ  R + + R   F+  ++C
Sbjct: 156 LEEVLATVDQLQSLVTN-FNDQQDEVDELRERITLKEERIQQMRNEASQRRFEFKTTIEC 214

Query: 171 LKPS 160
           L+ S
Sbjct: 215 LEES 218


>SPBC609.01 |||ribonuclease II |Schizosaccharomyces pombe|chr
            2|||Manual
          Length = 1157

 Score = 25.8 bits (54), Expect = 5.1
 Identities = 14/36 (38%), Positives = 21/36 (58%), Gaps = 1/36 (2%)
 Frame = +2

Query: 242  LHRGSVRRRVFILTLDGENIDIINFLQG-SR*LSAD 346
            L  G V+ + F+L +D E IDI+ +  G  R +S D
Sbjct: 970  LRSGLVKHKAFVLAVDQEYIDIVIYEFGLERRISLD 1005


>SPAC27D7.11c |||But2 family protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 463

 Score = 25.4 bits (53), Expect = 6.7
 Identities = 10/31 (32%), Positives = 17/31 (54%)
 Frame = -3

Query: 415 VILRSDTEVNPDGFSFGYETDNGISAQSSGS 323
           ++ +SDT     GFS G    +G S + +G+
Sbjct: 99  IVFKSDTNSTSSGFSVGEAISSGYSLKYNGT 129


>SPAC821.11 |pro1||gamma-glutamyl phosphate reductase Pro1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 451

 Score = 25.4 bits (53), Expect = 6.7
 Identities = 13/31 (41%), Positives = 17/31 (54%)
 Frame = -3

Query: 433 GSEADAVILRSDTEVNPDGFSFGYETDNGIS 341
           G +A  V   + T    DGF +GY T+ GIS
Sbjct: 365 GIDASGVYWNASTRF-ADGFRYGYGTEVGIS 394


>SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase
           Vas1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 950

 Score = 25.0 bits (52), Expect = 8.8
 Identities = 14/35 (40%), Positives = 19/35 (54%)
 Frame = -1

Query: 120 PKP*KSYKSRRFRAQRPPPKKSGLYFEHHTLSISI 16
           PKP KS ++  F    PPP  +G     H L+I+I
Sbjct: 50  PKP-KSIEAPMFPILLPPPNITGKLHIGHALTITI 83


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,353,759
Number of Sequences: 5004
Number of extensions: 42237
Number of successful extensions: 114
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 112
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 114
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 273658928
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -