Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner10h12r
(742 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g55410.1 68416.m06154 2-oxoglutarate dehydrogenase E1 compone... 186 2e-47
At5g65750.1 68418.m08274 2-oxoglutarate dehydrogenase E1 compone... 182 2e-46
At5g06680.1 68418.m00754 tubulin family protein similar to SP|Q9... 33 0.15
At1g59970.1 68414.m06755 matrixin family protein similar to SP|P... 31 1.1
At5g45550.1 68418.m05594 mob1/phocein family protein contains Pf... 29 3.2
At1g09610.1 68414.m01078 expressed protein contains Pfam profile... 29 3.2
At5g62070.1 68418.m07790 calmodulin-binding family protein conta... 29 4.3
At5g56500.1 68418.m07051 chaperonin, putative similar to SWISS-P... 29 4.3
At5g17570.1 68418.m02061 tatD-related deoxyribonuclease family p... 28 5.7
At4g35490.1 68417.m05043 ribosomal protein L11 family protein se... 28 5.7
At3g13470.1 68416.m01695 chaperonin, putative similar SWISS-PROT... 28 7.5
At5g19000.1 68418.m02257 speckle-type POZ protein-related contai... 27 9.9
At4g03340.1 68417.m00456 glycosyltransferase family 14 protein /... 27 9.9
>At3g55410.1 68416.m06154 2-oxoglutarate dehydrogenase E1 component,
putative / oxoglutarate decarboxylase, putative /
alpha-ketoglutaric dehydrogenase, putative similar to
SP|P20967 2-oxoglutarate dehydrogenase E1 component,
mitochondrial precursor (EC 1.2.4.2) (Alpha-ketoglutarate
dehydrogenase) {Saccharomyces cerevisiae}; contains Pfam
profiles PF02779: Transketolase, pyridine binding domain,
PF00676: Dehydrogenase E1 component
Length = 1017
Score = 186 bits (452), Expect = 2e-47
Identities = 106/219 (48%), Positives = 138/219 (63%), Gaps = 22/219 (10%)
Frame = -1
Query: 718 IASGESKWVRSNGLVMLLPHGYDGAASEHSSCRLERFLQLTD-SPESAPDSEAA------ 560
I+SGE+KW+R GLVMLLPHGYDG EHSS RLER+LQ++D +P PD E
Sbjct: 747 ISSGEAKWLRQTGLVMLLPHGYDGQGPEHSSARLERYLQMSDDNPYVIPDMEPTMRKQIQ 806
Query: 559 -C-LHVANPTTPAQYFHLLRRQVVRNYRKPLIIVAPKLLLRLADAVSPLSDF-------- 410
C + N TTPA YFH+LRRQ+ R++RKPLI++APK LLR D S LS+F
Sbjct: 807 ECNWQIVNATTPANYFHVLRRQIHRDFRKPLIVMAPKNLLRHKDCKSNLSEFDDVQGHPG 866
Query: 409 --APRTHFQPVIGD--GLADALK-VKRVIFVSGKHYYELHKERVRARLEDVAIVRVEALA 245
T F+ +I D +D + ++R++ SGK YYEL ER + DVAI RVE L
Sbjct: 867 FDKQGTRFKRLIKDQNDHSDLEEGIRRLVLCSGKVYYELDDERKKVGATDVAICRVEQLC 926
Query: 244 PFPLPELQMQLSKYKNATKFIWSQEEHRNMGAWSFMKPR 128
PFP +Q +L +Y NA + +W QEE NMGA+S++ PR
Sbjct: 927 PFPYDLIQRELKRYPNA-EIVWCQEEAMNMGAFSYISPR 964
>At5g65750.1 68418.m08274 2-oxoglutarate dehydrogenase E1 component,
putative / oxoglutarate decarboxylase, putative /
alpha-ketoglutaric dehydrogenase, putative similar to
SP|P20967 2-oxoglutarate dehydrogenase E1 component,
mitochondrial precursor (EC 1.2.4.2) (Alpha-ketoglutarate
dehydrogenase) {Saccharomyces cerevisiae}; contains Pfam
profiles PF02779: Transketolase, pyridine binding domain,
PF00676: Dehydrogenase E1 component
Length = 1025
Score = 182 bits (444), Expect = 2e-46
Identities = 113/266 (42%), Positives = 153/266 (57%), Gaps = 29/266 (10%)
Frame = -1
Query: 718 IASGESKWVRSNGLVMLLPHGYDGAASEHSSCRLERFLQLTDS-----PESAP----DSE 566
I+SGE+KW+R GLV+LLPHGYDG EHSS RLERFLQ++D PE P +
Sbjct: 751 ISSGEAKWLRQTGLVVLLPHGYDGQGPEHSSGRLERFLQMSDDNPYVIPEMDPTLRKQIQ 810
Query: 565 AACLHVANPTTPAQYFHLLRRQVVRNYRKPLIIVAPKLLLRLADAVSPLSDF-------- 410
V N TTPA YFH+LRRQ+ R++RKPLI++APK LLR VS LS+F
Sbjct: 811 ECNWQVVNVTTPANYFHVLRRQIHRDFRKPLIVMAPKNLLRHKQCVSNLSEFDDVKGHPG 870
Query: 409 --APRTHFQPVIGD--GLADALK-VKRVIFVSGKHYYELHKERVRARLEDVAIVRVEALA 245
T F+ +I D G +D + ++R++ SGK YYEL +ER ++ +DVAI RVE L
Sbjct: 871 FDKQGTRFKRLIKDQSGHSDLEEGIRRLVLCSGKVYYELDEERKKSETKDVAICRVEQLC 930
Query: 244 PFPLPELQMQLSKYKNATKFIWSQEEHRNMGAWSFMKPR-------FENLCGRKLVYAGR 86
PFP +Q +L +Y NA + +W QEE NMG + ++ R + + Y GR
Sbjct: 931 PFPYDLIQRELKRYPNA-EIVWCQEEPMNMGGYQYIALRLCTAMKALQRGNFNDIKYVGR 989
Query: 85 SEAPTPAVGASSLHRVEVEHVLKEPL 8
+ A G LH E ++K+ L
Sbjct: 990 LPSAATATGFYQLHVKEQTDLVKKAL 1015
>At5g06680.1 68418.m00754 tubulin family protein similar to
SP|Q96CW5 Gamma-tubulin complex component 3 {Homo
sapiens}
Length = 838
Score = 33.5 bits (73), Expect = 0.15
Identities = 18/51 (35%), Positives = 23/51 (45%), Gaps = 2/51 (3%)
Frame = -1
Query: 277 DVAIVRVEALAPFPLPELQMQLSKYKNATKFIW--SQEEHRNMGAWSFMKP 131
DV + EA P + LSKY F+W + EH +G W MKP
Sbjct: 578 DVFSLEYEARVPLDTVFTESVLSKYLRVFNFLWKLKRVEHALIGIWKTMKP 628
>At1g59970.1 68414.m06755 matrixin family protein similar to
SP|P29136 Metalloendoproteinase 1 precursor (EC
3.4.24.-) (SMEP1) {Glycine max}; contains InterPro
accession IPR001818: Matrixin
Length = 360
Score = 30.7 bits (66), Expect = 1.1
Identities = 14/43 (32%), Positives = 24/43 (55%)
Frame = +2
Query: 344 PLHFQRISQTVTDYWLEMSAGCEIGQGRDGIG*AEQQFRSYNY 472
PL F +Q + + ++ AGC IG+ +G+ +Q FR + Y
Sbjct: 31 PLQFLNATQNAWETFSKL-AGCHIGENINGLSKLKQYFRRFGY 72
>At5g45550.1 68418.m05594 mob1/phocein family protein contains Pfam
profile: PF03637 Mob1/phocein family
Length = 215
Score = 29.1 bits (62), Expect = 3.2
Identities = 14/44 (31%), Positives = 23/44 (52%)
Frame = -1
Query: 367 ADALKVKRVIFVSGKHYYELHKERVRARLEDVAIVRVEALAPFP 236
AD +++K+ I VS Y E + + +L+D + APFP
Sbjct: 98 ADGVQIKKPIEVSAPKYVEYLMDWIETQLDDETLFPQRLGAPFP 141
>At1g09610.1 68414.m01078 expressed protein contains Pfam profile
PF04669: Protein of unknown function (DUF579)
Length = 282
Score = 29.1 bits (62), Expect = 3.2
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = -2
Query: 255 KHSHPSPSRSYRCNSANTKTPRSLS 181
+H P +RS RC+ A K PRSL+
Sbjct: 37 EHQTPQETRSTRCSGACNKLPRSLA 61
>At5g62070.1 68418.m07790 calmodulin-binding family protein contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 403
Score = 28.7 bits (61), Expect = 4.3
Identities = 14/32 (43%), Positives = 18/32 (56%), Gaps = 1/32 (3%)
Frame = -2
Query: 258 WK-HSHPSPSRSYRCNSANTKTPRSLSGVKKS 166
WK H HP P RS R N + K +SL G + +
Sbjct: 248 WKPHYHPKPLRSERNNESPRKRQQSLLGPRST 279
>At5g56500.1 68418.m07051 chaperonin, putative similar to
SWISS-PROT:P08927- RuBisCO subunit binding-protein beta
subunit, chloroplast precursor (60 kDa chaperonin beta
subunit, CPN-60 beta) [Pisum sativum]; contains
Pfam:PF00118 domain, TCP-1/cpn60 chaperonin family
Length = 597
Score = 28.7 bits (61), Expect = 4.3
Identities = 21/73 (28%), Positives = 36/73 (49%), Gaps = 7/73 (9%)
Frame = -1
Query: 385 VIGDGLADALKVKRV-----IFVSGKHYYELHK--ERVRARLEDVAIVRVEALAPFPLPE 227
++GDG + + KRV + + + YE K ER+ VA+++V A L E
Sbjct: 382 IVGDGSTEEVVKKRVEQIKNLIEAAEQDYEKEKLNERIAKLSGGVAVIQVGAQTETELKE 441
Query: 226 LQMQLSKYKNATK 188
++++ NATK
Sbjct: 442 KKLRVEDALNATK 454
>At5g17570.1 68418.m02061 tatD-related deoxyribonuclease family
protein contains Pfam profile PF01026: TatD related
DNase
Length = 325
Score = 28.3 bits (60), Expect = 5.7
Identities = 12/33 (36%), Positives = 21/33 (63%), Gaps = 2/33 (6%)
Frame = -1
Query: 616 ERFLQLTDSPESAPDSEAACLHV--ANPTTPAQ 524
+R L TDSP++ P +E+ CL+ +P+ P +
Sbjct: 199 DRILLETDSPDALPKAESGCLYFVDGDPSLPEE 231
>At4g35490.1 68417.m05043 ribosomal protein L11 family protein
several ribosomal proteins L11
Length = 155
Score = 28.3 bits (60), Expect = 5.7
Identities = 14/41 (34%), Positives = 25/41 (60%)
Frame = +2
Query: 311 LVVMLTAYKDHPLHFQRISQTVTDYWLEMSAGCEIGQGRDG 433
+ V +TA+KD+ F S TV+ ++++ +AG + G R G
Sbjct: 63 MAVTITAFKDNSFEFTVKSPTVS-WYIKKAAGVDKGSTRPG 102
>At3g13470.1 68416.m01695 chaperonin, putative similar
SWISS-PROT:P21240- RuBisCO subunit binding-protein beta
subunit, chloroplast precursor (60 kDa chaperonin beta
subunit, CPN-60 beta) [Arabidopsis thaliana]; contains
Pfam:PF00118 domain, TCP-1/cpn60 chaperonin family
Length = 596
Score = 27.9 bits (59), Expect = 7.5
Identities = 21/73 (28%), Positives = 34/73 (46%), Gaps = 7/73 (9%)
Frame = -1
Query: 385 VIGDGLADALKVKRVIFV-----SGKHYYELHK--ERVRARLEDVAIVRVEALAPFPLPE 227
++GDG KRV+ + + YE K ER+ VA+++V A L E
Sbjct: 382 IVGDGTTQEAVNKRVVQIRNLIEQAEQDYEKEKLNERIAKLSGGVAVIQVGAQTETELKE 441
Query: 226 LQMQLSKYKNATK 188
++++ NATK
Sbjct: 442 KKLRVEDALNATK 454
>At5g19000.1 68418.m02257 speckle-type POZ protein-related contains
Pfam PF00651 : BTB/POZ domain; contains Pfam PF00917:
MATH domain; similar to Speckle-type POZ protein
(SP:O43791) [Homo sapiens]
Length = 442
Score = 27.5 bits (58), Expect = 9.9
Identities = 36/135 (26%), Positives = 52/135 (38%), Gaps = 2/135 (1%)
Frame = +2
Query: 140 ETPRSHISMLFLTPDKLRGVFVFAELHL*LREGEGCECFHPHNGHVLQPRPHALLMQLVV 319
E S++S+ + V EL L + G G H H G L P+ L +
Sbjct: 79 EDNSSYVSLFIALASEGADVRALFELTLVDQSGNGKHKVHSHFGRALDSGPYTLKYR--G 136
Query: 320 MLTAYKDHPLHFQRISQTVTDYWLEMS--AGCEIGQGRDGIG*AEQQFRSYNYQWLPVVT 493
+ YK F+R S +DY E S C +G + + R YN +PV
Sbjct: 137 SMWGYK---RFFRRSSLESSDYLKENSLLVRCRVGVVKS----VTEGPRYYN---IPVPV 186
Query: 494 DNLSPQQVEVLGRGR 538
NL Q +L G+
Sbjct: 187 SNLGQQLGNLLESGK 201
>At4g03340.1 68417.m00456 glycosyltransferase family 14 protein /
core-2/I-branching enzyme family protein contains Pfam
profile: PF02485 Core-2/I-Branching enzyme
Length = 448
Score = 27.5 bits (58), Expect = 9.9
Identities = 13/33 (39%), Positives = 20/33 (60%), Gaps = 1/33 (3%)
Frame = +2
Query: 299 LLMQLVVML-TAYKDHPLHFQRISQTVTDYWLE 394
L+ L V L T+Y + PL F +S+ DY++E
Sbjct: 51 LMSVLYVQLETSYVEEPLPFDNLSEETNDYFVE 83
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,345,504
Number of Sequences: 28952
Number of extensions: 334868
Number of successful extensions: 1077
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 1047
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1071
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1633819784
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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