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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fner10g20f
         (635 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_12544| Best HMM Match : DUF1218 (HMM E-Value=2.7)                   27   0.49 
SB_26361| Best HMM Match : fn3 (HMM E-Value=0)                         30   1.8  
SB_18417| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   2.4  
SB_13504| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   5.5  
SB_47160| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   5.5  
SB_30275| Best HMM Match : EGF_CA (HMM E-Value=1.3e-13)                28   7.3  
SB_26886| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   7.3  
SB_52928| Best HMM Match : PKD (HMM E-Value=0)                         27   9.7  
SB_43520| Best HMM Match : RNase_PH (HMM E-Value=0.00011)              27   9.7  

>SB_12544| Best HMM Match : DUF1218 (HMM E-Value=2.7)
          Length = 290

 Score = 27.1 bits (57), Expect(2) = 0.49
 Identities = 9/28 (32%), Positives = 18/28 (64%)
 Frame = +3

Query: 243 VNNLIIDKRRNTMEYCYKLWVGNGQDIV 326
           +++L++  +++   YC+     NGQDIV
Sbjct: 130 IHDLLLSLQKHLFAYCHNATSNNGQDIV 157



 Score = 23.4 bits (48), Expect(2) = 0.49
 Identities = 14/51 (27%), Positives = 22/51 (43%)
 Frame = +3

Query: 324 VKKYFPLSFRLIMAGNYVKLIYRNYNLALKLGSTTNPSNERIAYGDGVDKH 476
           ++ YF     L+M+G    L+  + +L L        S  R+  G G D H
Sbjct: 183 IRYYFACFQELLMSGPANSLLQSHLSLFLPCAGEILGSVYRLLVGHGSDTH 233


>SB_26361| Best HMM Match : fn3 (HMM E-Value=0)
          Length = 1898

 Score = 29.9 bits (64), Expect = 1.8
 Identities = 27/74 (36%), Positives = 34/74 (45%), Gaps = 5/74 (6%)
 Frame = +3

Query: 408  LKLGSTTNPSNERIAYGDGVDKHTELVSWKFITLWENNRVYFKAHNTKYNQYLKMS---- 575
            L+ GST   S++ IA       +T L S  F       R Y  AH T Y  YL +S    
Sbjct: 868  LESGSTLLASDDGIAPNKRTKVYTSLSSDVFY------RFYVYAHTT-YTTYLCLSVNAP 920

Query: 576  -TSTCNCNARDRVV 614
             T  C  N+RDRV+
Sbjct: 921  CTQACRLNSRDRVL 934


>SB_18417| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 441

 Score = 29.5 bits (63), Expect = 2.4
 Identities = 14/54 (25%), Positives = 21/54 (38%)
 Frame = -3

Query: 309 CRPTACSSTPWCSVSCQ*SDC*LHFERCCPGLGSRIPSSYVQHCRSHR*GCCCT 148
           C+   CS++  C  SC    C L+          +   + V  C+S   G  CT
Sbjct: 224 CQQVVCSASGKCDQSCDGEGCNLYCSEGAKTCNQKCQGACVTDCKSRWCGVTCT 277


>SB_13504| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 4924

 Score = 28.3 bits (60), Expect = 5.5
 Identities = 12/24 (50%), Positives = 14/24 (58%), Gaps = 2/24 (8%)
 Frame = -3

Query: 171  HR*GCCCTVCPRGL--G*KGSCCP 106
            H+ G  C VCPRG+    KG C P
Sbjct: 4552 HKNGFLCVVCPRGMYGNSKGECSP 4575


>SB_47160| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1806

 Score = 28.3 bits (60), Expect = 5.5
 Identities = 22/72 (30%), Positives = 32/72 (44%), Gaps = 2/72 (2%)
 Frame = +3

Query: 333  YFPLSFRLIMAGN--YVKLIYRNYNLALKLGSTTNPSNERIAYGDGVDKHTELVSWKFIT 506
            YFP   RLI       +     NY LA+ LG + + S   +  G+ VD+      W F  
Sbjct: 1387 YFPHMARLIDGQKPWCMSSSSSNYWLAIDLGVSVDVSAVEVK-GNDVDEDINQWIWDFSV 1445

Query: 507  LWENNRVYFKAH 542
             + N+ V +K H
Sbjct: 1446 EYSNDYVQWKQH 1457


>SB_30275| Best HMM Match : EGF_CA (HMM E-Value=1.3e-13)
          Length = 142

 Score = 27.9 bits (59), Expect = 7.3
 Identities = 14/43 (32%), Positives = 19/43 (44%)
 Frame = -1

Query: 164 EDAVVQFVLEVLVRRAHAVRG*FHDAGAGSEHAHCEHNEKFHF 36
           ED  V     + +R+   +R  F DA   +E   CE N   HF
Sbjct: 9   EDYTVNHSAAMAIRQCRCLRWLFQDAELKAEGLQCELNSDTHF 51


>SB_26886| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 6489

 Score = 27.9 bits (59), Expect = 7.3
 Identities = 23/79 (29%), Positives = 36/79 (45%), Gaps = 2/79 (2%)
 Frame = +1

Query: 109  TA*ALLTKTSRTNCTTASSPVTTTVLYVRAWNTRAKARAASFKM*LTI*SLTRD--GTPW 282
            TA A+   T+    TTA    TTT   V A  T A   A++  +  T  ++      TP 
Sbjct: 1206 TAAAVTASTASVVTTTAEKATTTTAAMVEATTTEAAVTASTASVVTTTATIASSTITTPS 1265

Query: 283  STATSCGSATDRILSKSTS 339
             +A S  +A+   +S +T+
Sbjct: 1266 LSAISTTTASSVQVSAATN 1284


>SB_52928| Best HMM Match : PKD (HMM E-Value=0)
          Length = 1624

 Score = 27.5 bits (58), Expect = 9.7
 Identities = 32/123 (26%), Positives = 54/123 (43%), Gaps = 3/123 (2%)
 Frame = -2

Query: 370 FPAMMSLKLNGKYFLTISCPLPTHSL*QYSMVFRLLSMIRLLTTF*TMLP---WPWLSYS 200
           FPAM +L +NG + +++       SL ++    R     R L     M P     W   S
Sbjct: 519 FPAMAALTMNGSHAVSLWSYGDGSSLKKHESGPRQFITSRHLYAHTGMFPVTVTVWNRLS 578

Query: 199 KLLRTALS*SPVRMLLYSLSSRSWLEGLMLSADSSTTPALAASTHIANTTRSFILLGAFS 20
           K   TAL+   V++ + ++S  S    L  S  +S T  +  ++H   T  S+ ++    
Sbjct: 579 KKNETALAYVSVQVPVTNISVPS---DLTASLGNSVTFTVKITSHETPTNASYYIIYGNG 635

Query: 19  DRS 11
           +RS
Sbjct: 636 ERS 638


>SB_43520| Best HMM Match : RNase_PH (HMM E-Value=0.00011)
          Length = 972

 Score = 27.5 bits (58), Expect = 9.7
 Identities = 17/46 (36%), Positives = 22/46 (47%)
 Frame = +1

Query: 43  NFSLCSQCACSLPAPASWNYPRTA*ALLTKTSRTNCTTASSPVTTT 180
           ++  C  C CS  A        TA A  T T+ T  TTA++  TTT
Sbjct: 511 HYYCCYYCCCSCTAT-------TATATTTATATTTATTAATATTTT 549


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,482,817
Number of Sequences: 59808
Number of extensions: 379422
Number of successful extensions: 1289
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 1157
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1289
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1596754500
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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