BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fner10g18r (751 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g05170.1 68415.m00544 vacuolar protein sorting 11 family prot... 31 0.82 At1g64150.1 68414.m07267 expressed protein contains Pfam profile... 29 2.5 At1g77240.1 68414.m08996 AMP-binding protein, putative strong si... 29 3.3 At5g49160.1 68418.m06085 DNA (cytosine-5-)-methyltransferase (AT... 29 4.4 At4g13290.1 68417.m02078 cytochrome P450 71A19, putative (CYP71A... 29 4.4 At1g47310.1 68414.m05238 expressed protein 28 5.8 At3g62000.1 68416.m06963 O-methyltransferase family 3 protein se... 28 7.6 At3g12775.1 68416.m01595 ubiquitin-conjugating enzyme family pro... 28 7.6 At3g04160.1 68416.m00440 expressed protein ; expression supporte... 28 7.6 At2g25290.1 68415.m03025 octicosapeptide/Phox/Bem1p (PB1) domain... 28 7.6 >At2g05170.1 68415.m00544 vacuolar protein sorting 11 family protein / VPS11 family protein similar to Vacuolar protein sorting 11 (hVPS11) (PP3476) (Swiss-Prot:Q9H270) [Homo sapiens]; similar to Vacuolar biogenesis protein END1 (PEP5 protein) (Vacuolar protein sorting 11) (Swiss-Prot:P12868) [Saccharomyces cerevisiae] Length = 932 Score = 31.1 bits (67), Expect = 0.82 Identities = 21/85 (24%), Positives = 40/85 (47%), Gaps = 4/85 (4%) Frame = -3 Query: 689 IGEYETAIAKCSEYLKEKKGEVIKEAVKRLIENGKRNTMDFAYQLWTKDG--KEIVKSYF 516 +G Y+ A+ S + G I++ K LIE+ + T+D +L T+ G + S Sbjct: 498 LGNYDEALQYVSSLEPSQAGVTIEQYGKILIEHKPKETIDILMRLCTEQGIPNGVFLSML 557 Query: 515 --PIQFRVIFTEQTVKLINKRDHHA 447 P+ F +F + L++ + +A Sbjct: 558 PSPVDFITVFVQHPHSLMHFLERYA 582 >At1g64150.1 68414.m07267 expressed protein contains Pfam profile PF01169: Uncharacterized protein family UPF0016 Length = 370 Score = 29.5 bits (63), Expect = 2.5 Identities = 13/35 (37%), Positives = 20/35 (57%) Frame = +3 Query: 228 GVSGAITVDDTVITRTFRVIELQVLFVFGGHDLEV 332 G G +T+ V+ RTF ++ + F FGG DL + Sbjct: 197 GALGIMTIISVVLGRTFHYVDEVLPFRFGGTDLPI 231 >At1g77240.1 68414.m08996 AMP-binding protein, putative strong similarity to AMP-binding protein GI:1903034 from [Brassica napus]; contains Pfam AMP-binding domain PF00501 Length = 545 Score = 29.1 bits (62), Expect = 3.3 Identities = 19/67 (28%), Positives = 32/67 (47%), Gaps = 1/67 (1%) Frame = -3 Query: 530 VKSYFPIQFRVIFTEQTVKLINKR-DHHALKLIDQQNHNKIAFGDSKDKTSKKVSWKFTP 354 V S + +QF V + + IN R D HAL ++ + + +K+ F D + + F P Sbjct: 76 VPSVYELQFAVPMSGAVLNNINPRLDAHALSVLLRHSESKLVFVDHHSSSLVLEAVSFLP 135 Query: 353 VLENNRV 333 E R+ Sbjct: 136 KDERPRL 142 >At5g49160.1 68418.m06085 DNA (cytosine-5-)-methyltransferase (ATHIM) identical to SP|P34881 DNA (cytosine-5)-methyltransferase AthI (EC 2.1.1.37) {Arabidopsis thaliana} Length = 1534 Score = 28.7 bits (61), Expect = 4.4 Identities = 11/23 (47%), Positives = 15/23 (65%) Frame = +2 Query: 545 PLSITGRRSPWCSSCRFRSDASR 613 PLS GR S +C+SC+ R D + Sbjct: 869 PLSDIGRSSGFCTSCKIREDEEK 891 >At4g13290.1 68417.m02078 cytochrome P450 71A19, putative (CYP71A19) Identical to Cytochrome P450 (SP:Q9T0K0) [Arabidopsis thaliana]; similar to cytochrome P450LXXIA1, Persea americana, M32885 Length = 490 Score = 28.7 bits (61), Expect = 4.4 Identities = 11/31 (35%), Positives = 19/31 (61%) Frame = +3 Query: 447 GVMVPFVYELDSLLGEDHSKLDGEVRFDDFL 539 G +P + +D + G+DH + + RFD+FL Sbjct: 221 GEYIPSLSWIDKIRGQDHKMEEVDKRFDEFL 251 >At1g47310.1 68414.m05238 expressed protein Length = 395 Score = 28.3 bits (60), Expect = 5.8 Identities = 19/67 (28%), Positives = 35/67 (52%) Frame = -3 Query: 491 TEQTVKLINKRDHHALKLIDQQNHNKIAFGDSKDKTSKKVSWKFTPVLENNRVYFKIMST 312 T QTV + + A ++ Q + + G ++ SKKV+W+ P +E RV+F++ + Sbjct: 286 TRQTVSSVTAK-LKASGMVRFQLEIERSIGKNESVISKKVAWRTKPKIE--RVWFEVTAK 342 Query: 311 EDKQYLK 291 + LK Sbjct: 343 IEGDKLK 349 >At3g62000.1 68416.m06963 O-methyltransferase family 3 protein several O-methyltransferases - different species; contains Pfam 01596 O-methyltransferase domain Length = 278 Score = 27.9 bits (59), Expect = 7.6 Identities = 26/126 (20%), Positives = 56/126 (44%), Gaps = 2/126 (1%) Frame = -3 Query: 722 VLAEQLYMSVVIGEYETAIAKCSEYLKEKKGEVIKEAVKRLIENGKRNTMDFAYQLWTKD 543 V E+ S+ + + +A S + K+G + E++K +I+NG+ + DFA+ Sbjct: 147 VACERDSNSLEVAKRYYELAGVSHKVNVKQG-LAAESLKSMIQNGEGASYDFAF---VDA 202 Query: 542 GKEIVKSYFPIQFRVIFTEQTVKLINKRDHHALK--LIDQQNHNKIAFGDSKDKTSKKVS 369 K + + YF + +++ + + N H + +++ I + K K+VS Sbjct: 203 DKRMYQDYFELLLQLVRVGGVIVMDNVLWHGRVSDPMVNDAKTISIRNFNKKLMDDKRVS 262 Query: 368 WKFTPV 351 P+ Sbjct: 263 ISMVPI 268 >At3g12775.1 68416.m01595 ubiquitin-conjugating enzyme family protein contains Pfam profile PF00179: Ubiquitin-conjugating enzyme Length = 362 Score = 27.9 bits (59), Expect = 7.6 Identities = 16/58 (27%), Positives = 31/58 (53%), Gaps = 1/58 (1%) Frame = -3 Query: 401 DSKDKTSKKVSWKFTPVLENNRVYF-KIMSTEDKQYLKLDNTKGSSDDRIIYGDSTAD 231 D +++ KK+ WK E N+VY +I++++ K L+ K + YG S+++ Sbjct: 277 DMEEERDKKMFWKMYFAFEGNKVYCEQILNSDLKAELEKLKEKEYTLSDYYYGSSSSN 334 >At3g04160.1 68416.m00440 expressed protein ; expression supported by MPSS Length = 712 Score = 27.9 bits (59), Expect = 7.6 Identities = 11/37 (29%), Positives = 18/37 (48%) Frame = -2 Query: 615 SREASDRKRQEEHHGLRLPVMDKGWKGNRQILLPHPV 505 SR ++E G +P DK WKG + + +P+ Sbjct: 563 SRSPIGNDQKESDFGYSIPSTDKQWKGENRADIEYPI 599 >At2g25290.1 68415.m03025 octicosapeptide/Phox/Bem1p (PB1) domain-containing protein / tetratricopeptide repeat (TPR)-containing protein low similarity to SP|Q99614 Tetratricopeptide repeat protein 1 {Homo sapiens}; contains Pfam profiles PF00564: PB1 domain, PF00515: TPR Domain Length = 697 Score = 27.9 bits (59), Expect = 7.6 Identities = 17/49 (34%), Positives = 25/49 (51%) Frame = -3 Query: 704 YMSVVIGEYETAIAKCSEYLKEKKGEVIKEAVKRLIENGKRNTMDFAYQ 558 YM + +GEY AI +C+ L E K +KR N +DFA++ Sbjct: 100 YMQMGLGEYPNAINECNLAL-EASPRFSKALLKRARCYEALNKLDFAFR 147 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,880,938 Number of Sequences: 28952 Number of extensions: 370632 Number of successful extensions: 1331 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 1283 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1330 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1663169840 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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