Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner10g18f
(612 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_55418| Best HMM Match : GWT1 (HMM E-Value=1.5) 30 1.7
SB_23612| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 1.7
SB_58268| Best HMM Match : Extensin_2 (HMM E-Value=0.002) 30 1.7
SB_39819| Best HMM Match : Extensin_2 (HMM E-Value=1) 30 1.7
SB_33746| Best HMM Match : MAM (HMM E-Value=8.4e-13) 29 2.2
SB_48608| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 3.0
SB_40674| Best HMM Match : Extensin_2 (HMM E-Value=0.19) 29 3.0
SB_31262| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 3.0
SB_49054| Best HMM Match : Chordopox_A30L (HMM E-Value=4.1) 29 3.9
SB_52408| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 5.2
SB_33920| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 5.2
SB_27872| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 5.2
SB_10252| Best HMM Match : tRNA-synt_2b (HMM E-Value=3.2e-32) 28 5.2
SB_26915| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 5.2
SB_20304| Best HMM Match : Vicilin_N (HMM E-Value=4.6) 28 6.9
SB_12810| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 6.9
SB_45611| Best HMM Match : p450 (HMM E-Value=0) 27 9.1
SB_27646| Best HMM Match : CbiK (HMM E-Value=2.2) 27 9.1
SB_9731| Best HMM Match : Lig_chan (HMM E-Value=3.7e-05) 27 9.1
>SB_55418| Best HMM Match : GWT1 (HMM E-Value=1.5)
Length = 260
Score = 29.9 bits (64), Expect = 1.7
Identities = 14/45 (31%), Positives = 22/45 (48%)
Frame = -1
Query: 285 W*AKSMVFLLPFSIRRFTASLITSPFFSFRYSEHLAIAVSYSPMT 151
W + +F++ SI + A+L F F H+ AV+YS T
Sbjct: 127 WQSSRFMFIMRLSILSYAAALFHDQGFEFSLGLHILAAVAYSVRT 171
>SB_23612| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1021
Score = 29.9 bits (64), Expect = 1.7
Identities = 21/58 (36%), Positives = 27/58 (46%), Gaps = 2/58 (3%)
Frame = +2
Query: 164 YETAIAKCSEYLKEK--KGEVIKEAVKRLIENGKRNTMDFAYQLWTKDGKEIVKSYFP 331
Y + I K E+ KEK KG+ K R E GKR A +D E +K +FP
Sbjct: 261 YPSLIDKLQEHEKEKELKGKRKKSKTSRSPEKGKREEEKDALLKQEEDEIERMKQFFP 318
>SB_58268| Best HMM Match : Extensin_2 (HMM E-Value=0.002)
Length = 458
Score = 29.9 bits (64), Expect = 1.7
Identities = 14/34 (41%), Positives = 14/34 (41%)
Frame = +3
Query: 447 PKTKPARKSPGSLPPCWKTTEYTSRSCPPKTNST 548
P TK PP K T Y R CPP T T
Sbjct: 136 PLTKCTNYRKRRCPPLTKCTNYRKRRCPPLTKCT 169
Score = 29.9 bits (64), Expect = 1.7
Identities = 14/34 (41%), Positives = 14/34 (41%)
Frame = +3
Query: 447 PKTKPARKSPGSLPPCWKTTEYTSRSCPPKTNST 548
P TK PP K T Y R CPP T T
Sbjct: 150 PLTKCTNYRKRRCPPLTKCTNYRKRRCPPLTKCT 183
Score = 29.9 bits (64), Expect = 1.7
Identities = 14/34 (41%), Positives = 14/34 (41%)
Frame = +3
Query: 447 PKTKPARKSPGSLPPCWKTTEYTSRSCPPKTNST 548
P TK PP K T Y R CPP T T
Sbjct: 164 PLTKCTNYRKRRCPPLTKCTNYRKRRCPPLTKCT 197
Score = 29.9 bits (64), Expect = 1.7
Identities = 14/34 (41%), Positives = 14/34 (41%)
Frame = +3
Query: 447 PKTKPARKSPGSLPPCWKTTEYTSRSCPPKTNST 548
P TK PP K T Y R CPP T T
Sbjct: 178 PLTKCTNYRKRRCPPLTKCTNYRKRRCPPLTRCT 211
Score = 29.1 bits (62), Expect = 3.0
Identities = 14/34 (41%), Positives = 14/34 (41%)
Frame = +3
Query: 447 PKTKPARKSPGSLPPCWKTTEYTSRSCPPKTNST 548
P TK PP K T Y R CPP T T
Sbjct: 220 PLTKCTYYRKRRCPPLTKCTNYRKRRCPPLTRCT 253
Score = 29.1 bits (62), Expect = 3.0
Identities = 13/34 (38%), Positives = 14/34 (41%)
Frame = +3
Query: 447 PKTKPARKSPGSLPPCWKTTEYTSRSCPPKTNST 548
P TK PP + T Y R CPP T T
Sbjct: 290 PLTKRTNYRKKRYPPLTRCTNYRKRRCPPLTRCT 323
Score = 28.7 bits (61), Expect = 3.9
Identities = 13/34 (38%), Positives = 14/34 (41%)
Frame = +3
Query: 447 PKTKPARKSPGSLPPCWKTTEYTSRSCPPKTNST 548
P T+ PP K T Y R CPP T T
Sbjct: 122 PLTRCTNYRKRRCPPLTKCTNYRKRRCPPLTKCT 155
Score = 28.7 bits (61), Expect = 3.9
Identities = 13/34 (38%), Positives = 14/34 (41%)
Frame = +3
Query: 447 PKTKPARKSPGSLPPCWKTTEYTSRSCPPKTNST 548
P TK PP + T Y R CPP T T
Sbjct: 192 PLTKCTNYRKRRCPPLTRCTNYRKRRCPPLTKCT 225
Score = 28.7 bits (61), Expect = 3.9
Identities = 13/34 (38%), Positives = 14/34 (41%)
Frame = +3
Query: 447 PKTKPARKSPGSLPPCWKTTEYTSRSCPPKTNST 548
P TK PP + T Y R CPP T T
Sbjct: 234 PLTKCTNYRKRRCPPLTRCTNYLKRRCPPLTRCT 267
Score = 28.3 bits (60), Expect = 5.2
Identities = 11/21 (52%), Positives = 11/21 (52%)
Frame = +3
Query: 486 PPCWKTTEYTSRSCPPKTNST 548
PP K T Y R CPP T T
Sbjct: 275 PPLTKCTNYLKRRCPPLTKRT 295
Score = 28.3 bits (60), Expect = 5.2
Identities = 14/34 (41%), Positives = 14/34 (41%)
Frame = +3
Query: 447 PKTKPARKSPGSLPPCWKTTEYTSRSCPPKTNST 548
P TK PP K T Y R CPP T T
Sbjct: 416 PLTKGNYYRKRRCPPLTKGTNYRKRRCPPLTRCT 449
Score = 27.9 bits (59), Expect = 6.9
Identities = 14/37 (37%), Positives = 14/37 (37%)
Frame = +3
Query: 429 KLHSVTPKTKPARKSPGSLPPCWKTTEYTSRSCPPKT 539
K P TK PP K T Y R CPP T
Sbjct: 46 KTRRCNPLTKGNYYRKRRCPPLTKCTNYRKRRCPPLT 82
Score = 27.9 bits (59), Expect = 6.9
Identities = 13/34 (38%), Positives = 14/34 (41%)
Frame = +3
Query: 447 PKTKPARKSPGSLPPCWKTTEYTSRSCPPKTNST 548
P T+ PP K T Y R CPP T T
Sbjct: 206 PLTRCTNYRKRRCPPLTKCTYYRKRRCPPLTKCT 239
>SB_39819| Best HMM Match : Extensin_2 (HMM E-Value=1)
Length = 259
Score = 29.9 bits (64), Expect = 1.7
Identities = 14/34 (41%), Positives = 14/34 (41%)
Frame = +3
Query: 447 PKTKPARKSPGSLPPCWKTTEYTSRSCPPKTNST 548
P TK PP K T Y R CPP T T
Sbjct: 189 PLTKCTNYRKRRCPPLTKCTNYRKRRCPPLTRCT 222
Score = 29.5 bits (63), Expect = 2.2
Identities = 14/34 (41%), Positives = 14/34 (41%)
Frame = +3
Query: 447 PKTKPARKSPGSLPPCWKTTEYTSRSCPPKTNST 548
P TK PP K T Y R CPP T T
Sbjct: 147 PLTKCTNYLKRRCPPLTKCTNYRKRRCPPLTRCT 180
Score = 28.7 bits (61), Expect = 3.9
Identities = 13/34 (38%), Positives = 14/34 (41%)
Frame = +3
Query: 447 PKTKPARKSPGSLPPCWKTTEYTSRSCPPKTNST 548
P TK PP + T Y R CPP T T
Sbjct: 35 PLTKCTNYRKRRCPPLTRCTNYRKRRCPPLTRCT 68
Score = 28.7 bits (61), Expect = 3.9
Identities = 13/34 (38%), Positives = 14/34 (41%)
Frame = +3
Query: 447 PKTKPARKSPGSLPPCWKTTEYTSRSCPPKTNST 548
P TK PP + T Y R CPP T T
Sbjct: 161 PLTKCTNYRKRRCPPLTRCTNYRKRRCPPLTKCT 194
Score = 28.7 bits (61), Expect = 3.9
Identities = 13/34 (38%), Positives = 14/34 (41%)
Frame = +3
Query: 447 PKTKPARKSPGSLPPCWKTTEYTSRSCPPKTNST 548
P T+ PP K T Y R CPP T T
Sbjct: 175 PLTRCTNYRKRRCPPLTKCTNYRKRRCPPLTKCT 208
Score = 28.3 bits (60), Expect = 5.2
Identities = 11/21 (52%), Positives = 11/21 (52%)
Frame = +3
Query: 486 PPCWKTTEYTSRSCPPKTNST 548
PP K T Y R CPP T T
Sbjct: 34 PPLTKCTNYRKRRCPPLTRCT 54
Score = 28.3 bits (60), Expect = 5.2
Identities = 11/21 (52%), Positives = 11/21 (52%)
Frame = +3
Query: 486 PPCWKTTEYTSRSCPPKTNST 548
PP K T Y R CPP T T
Sbjct: 146 PPLTKCTNYLKRRCPPLTKCT 166
Score = 27.5 bits (58), Expect = 9.1
Identities = 12/34 (35%), Positives = 14/34 (41%)
Frame = +3
Query: 447 PKTKPARKSPGSLPPCWKTTEYTSRSCPPKTNST 548
P T+ PP + T Y R CPP T T
Sbjct: 49 PLTRCTNYRKRRCPPLTRCTNYRKRRCPPLTKCT 82
>SB_33746| Best HMM Match : MAM (HMM E-Value=8.4e-13)
Length = 618
Score = 29.5 bits (63), Expect = 2.2
Identities = 27/93 (29%), Positives = 44/93 (47%), Gaps = 7/93 (7%)
Frame = -1
Query: 573 EPFVLSSFRYCLSSVDMILKYTL-LFSN----TGVNFQE--TFLLVLSLESPNAILLWFC 415
+P S+ C +I Y + L SN TGV F E + L L S +++ +++
Sbjct: 485 KPVDTLSYLVCFGCPPVISSYNIHLGSNEEQTTGVQFDEFAVYCKPLELASVDSMYIFYK 544
Query: 414 WSINLRA*WSLLFMSLTVCSVKITLNWMGK*DL 316
++ LRA ++LLF T +L + G DL
Sbjct: 545 GALGLRANYTLLFPDKTWSYYWTSLEYSGLLDL 577
>SB_48608| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 321
Score = 29.1 bits (62), Expect = 3.0
Identities = 15/45 (33%), Positives = 29/45 (64%)
Frame = -1
Query: 591 MIRSSLEPFVLSSFRYCLSSVDMILKYTLLFSNTGVNFQETFLLV 457
MI + EP +L+ +Y ++S+ +K+T +S T +NF +T++ V
Sbjct: 106 MIWTHDEPSLLNFLQY-INSIHPTIKFTHEYSPTRINFLDTYIHV 149
>SB_40674| Best HMM Match : Extensin_2 (HMM E-Value=0.19)
Length = 291
Score = 29.1 bits (62), Expect = 3.0
Identities = 13/36 (36%), Positives = 16/36 (44%)
Frame = +3
Query: 441 VTPKTKPARKSPGSLPPCWKTTEYTSRSCPPKTNST 548
++P TK PP + T Y R CPP T T
Sbjct: 1 MSPLTKRTYYRKKRYPPLTRCTNYRKRRCPPLTRCT 36
Score = 28.7 bits (61), Expect = 3.9
Identities = 13/34 (38%), Positives = 14/34 (41%)
Frame = +3
Query: 447 PKTKPARKSPGSLPPCWKTTEYTSRSCPPKTNST 548
P TK PP + T Y R CPP T T
Sbjct: 226 PLTKCTNYRKRRCPPLTRCTNYLKRRCPPLTKCT 259
Score = 28.3 bits (60), Expect = 5.2
Identities = 11/21 (52%), Positives = 11/21 (52%)
Frame = +3
Query: 486 PPCWKTTEYTSRSCPPKTNST 548
PP K T Y R CPP T T
Sbjct: 225 PPLTKCTNYRKRRCPPLTRCT 245
Score = 28.3 bits (60), Expect = 5.2
Identities = 11/21 (52%), Positives = 11/21 (52%)
Frame = +3
Query: 486 PPCWKTTEYTSRSCPPKTNST 548
PP K T Y R CPP T T
Sbjct: 253 PPLTKCTNYLKRRCPPLTKCT 273
Score = 27.9 bits (59), Expect = 6.9
Identities = 13/31 (41%), Positives = 13/31 (41%)
Frame = +3
Query: 447 PKTKPARKSPGSLPPCWKTTEYTSRSCPPKT 539
P TK PP K T Y R CPP T
Sbjct: 254 PLTKCTNYLKRRCPPLTKCTNYRKRRCPPLT 284
>SB_31262| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 358
Score = 29.1 bits (62), Expect = 3.0
Identities = 14/31 (45%), Positives = 19/31 (61%)
Frame = +3
Query: 438 SVTPKTKPARKSPGSLPPCWKTTEYTSRSCP 530
S++P + R S GSL P +T+ TSRS P
Sbjct: 179 SISPASPALRSSLGSLAPTSRTSTPTSRSTP 209
>SB_49054| Best HMM Match : Chordopox_A30L (HMM E-Value=4.1)
Length = 432
Score = 28.7 bits (61), Expect = 3.9
Identities = 27/117 (23%), Positives = 51/117 (43%)
Frame = +1
Query: 202 GKEGRGYQGSREASDRKRQEEHHGLRLPVMDKGWKGNRQILLPHPV*SDLHRADCQAHKQ 381
G+ R G RE SDR+++ E R D+G G+R+ R D AH
Sbjct: 151 GESSRIRSGDREKSDREKEREKERRRRRHEDEG-SGDRR----------RERRDKDAHDP 199
Query: 382 KGPSRPQVDRPTKPQQNCIR*LQRQNQQESLLEVYPRVGKQQSILQDHVHRRQTVPE 552
+ P R + D K ++ ++ ++S L+ R+ +++ L + + + + E
Sbjct: 200 EDPKRREKDDKKKSRERSKG--DEEHTKDSKLDEEDRIRRREKELLETLKQEEEAVE 254
>SB_52408| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 673
Score = 28.3 bits (60), Expect = 5.2
Identities = 14/63 (22%), Positives = 28/63 (44%)
Frame = +1
Query: 346 DLHRADCQAHKQKGPSRPQVDRPTKPQQNCIR*LQRQNQQESLLEVYPRVGKQQSILQDH 525
DLH + Q +Q G + + + QQ ++ Q+Q QQ+ L + + + L
Sbjct: 558 DLHEEEVQHQQQFGLQEQSLGQEQRKQQQQLQQQQQQKQQQQLQKKQQKQSSMEEKLSSE 617
Query: 526 VHR 534
+ +
Sbjct: 618 IEK 620
>SB_33920| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1278
Score = 28.3 bits (60), Expect = 5.2
Identities = 19/45 (42%), Positives = 22/45 (48%)
Frame = +1
Query: 424 QQNCIR*LQRQNQQESLLEVYPRVGKQQSILQDHVHRRQTVPEAR 558
QQ C LQR +S LE YP GK L +H RQ V + R
Sbjct: 925 QQMCASDLQRLPYCQSQLEKYPGQGKLVPCLLEH---RQNVTDTR 966
>SB_27872| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 975
Score = 28.3 bits (60), Expect = 5.2
Identities = 13/30 (43%), Positives = 18/30 (60%)
Frame = +2
Query: 194 YLKEKKGEVIKEAVKRLIENGKRNTMDFAY 283
+ +E+ GE EA KRLI+ GK+ M Y
Sbjct: 676 FREEEDGESFAEAKKRLIKQGKQQIMFLLY 705
>SB_10252| Best HMM Match : tRNA-synt_2b (HMM E-Value=3.2e-32)
Length = 734
Score = 28.3 bits (60), Expect = 5.2
Identities = 17/40 (42%), Positives = 21/40 (52%)
Frame = +2
Query: 482 FTPVLENNRVYFKIMSTEDKQYLKLDNTKGSSDDRIIYGD 601
FT + E K MS E+KQ K DN KG S+ + GD
Sbjct: 204 FTEMEEKIDEKVKEMSLEEKQPQKKDNQKGKSEKKKGKGD 243
>SB_26915| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 3934
Score = 28.3 bits (60), Expect = 5.2
Identities = 18/69 (26%), Positives = 33/69 (47%), Gaps = 1/69 (1%)
Frame = +2
Query: 383 RDHHALKLIDQQNHNK-IAFGDSKDKTSKKVSWKFTPVLENNRVYFKIMSTEDKQYLKLD 559
R HH+ + + K + SKD T+ VS + + E ++ M+T +K +K+
Sbjct: 418 RRHHSFGGLPLEYREKRLRESSSKDNTNTSVSLEIKKIREAQKLIDAAMATSEKS-IKVG 476
Query: 560 NTKGSSDDR 586
+KG D +
Sbjct: 477 ESKGLWDSK 485
>SB_20304| Best HMM Match : Vicilin_N (HMM E-Value=4.6)
Length = 261
Score = 27.9 bits (59), Expect = 6.9
Identities = 12/33 (36%), Positives = 15/33 (45%)
Frame = +3
Query: 444 TPKTKPARKSPGSLPPCWKTTEYTSRSCPPKTN 542
+PKT P WKT+ T R PKT+
Sbjct: 41 SPKTSPKTSPKTGRKTSWKTSTKTGRKTSPKTS 73
>SB_12810| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 546
Score = 27.9 bits (59), Expect = 6.9
Identities = 16/74 (21%), Positives = 32/74 (43%), Gaps = 3/74 (4%)
Frame = +2
Query: 164 YETAIAKCSEYLKEKKGEVIKEAVKRLIENGKRNTMDFAYQ---LWTKDGKEIVKSYFPI 334
Y + KC Y+ + +G ++ + + KR M + LWT ++I K Y
Sbjct: 456 YPAGLTKCLSYVADDRGRALRLVLTKT--EAKRIPMLNRPEEGFLWTSTQQQITKGYESA 513
Query: 335 QFRVIFTEQTVKLI 376
R+ + T +++
Sbjct: 514 HIRLARNDSTTRIV 527
>SB_45611| Best HMM Match : p450 (HMM E-Value=0)
Length = 847
Score = 27.5 bits (58), Expect = 9.1
Identities = 22/100 (22%), Positives = 45/100 (45%), Gaps = 1/100 (1%)
Frame = +2
Query: 158 GEYETAIAKCSEYLKEKKGEVIKE-AVKRLIENGKRNTMDFAYQLWTKDGKEIVKSYFPI 334
G ++ A +K+K +VI A+K + ++F + WT ++ P+
Sbjct: 402 GTFKKAKRVIKHAVKDKSSDVIDRWAIKEALA---LQPVEFGGRPWTVSFDLALRGERPL 458
Query: 335 QFRVIFTEQTVKLINKRDHHALKLIDQQNHNKIAFGDSKD 454
F + VKL K H ALK+ Q+ + ++ ++++
Sbjct: 459 VFEDY--GRHVKLHRKMGHRALKVYSQKKYQQVIIQEAEE 496
>SB_27646| Best HMM Match : CbiK (HMM E-Value=2.2)
Length = 423
Score = 27.5 bits (58), Expect = 9.1
Identities = 37/134 (27%), Positives = 59/134 (44%), Gaps = 9/134 (6%)
Frame = +2
Query: 197 LKEKKGEVIKEAVKRLIENGKRNTMDF--AYQLWTKD---GKEIVKSYFPIQFRVI-FTE 358
LKEK E IKE+ K + + + + MDF + L + GK + + Q RV+ F +
Sbjct: 233 LKEKFEEGIKESKKEIEDADQYDKMDFPLSDNLPVPEVAYGKHLKRKVLDRQGRVVSFFD 292
Query: 359 QTVKLINKRDHHALKLIDQQNHNKIA---FGDSKDKTSKKVSWKFTPVLENNRVYFKIMS 529
L K D AL+L ++ A + +SKD VSW + + + +I
Sbjct: 293 ---GLFTKGDLDALRLYLLHYNSAYAYQGYDESKDTEHDNVSW-IAGIKVSKFIKSRIWK 348
Query: 530 TEDKQYLKLDNTKG 571
T + + N KG
Sbjct: 349 TINSAVEYISNEKG 362
>SB_9731| Best HMM Match : Lig_chan (HMM E-Value=3.7e-05)
Length = 435
Score = 27.5 bits (58), Expect = 9.1
Identities = 20/78 (25%), Positives = 33/78 (42%), Gaps = 1/78 (1%)
Frame = +2
Query: 374 INKRDHHALKLIDQQNHNKIAFGDSKDKTSKKVSWKFTPVLENNRVYFKIMST-EDKQYL 550
+NK +H + D + IA+G + + ++ + PV E Y + T ED
Sbjct: 22 VNKYEHPIKSIEDLADQQGIAYGTAPGQLAEMLKTASLPVYEKLWRYIETHGTLEDNATY 81
Query: 551 KLDNTKGSSDDRIIYGDS 604
+D + S DD DS
Sbjct: 82 AIDKVR-SRDDYAFIWDS 98
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 19,759,943
Number of Sequences: 59808
Number of extensions: 438265
Number of successful extensions: 1593
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 1423
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1592
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1499981500
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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