BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fner10g16f (635 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g29375.1 68416.m03690 XH domain-containing protein contains P... 31 0.85 At5g46740.1 68418.m05758 ubiquitin-specific protease 21 (UBP21) ... 30 1.5 At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-contain... 30 1.5 At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-contain... 30 1.5 At4g17895.1 68417.m02667 ubiquitin-specific protease 20, putativ... 29 2.0 At5g12150.1 68418.m01426 pleckstrin homology (PH) domain-contain... 28 4.5 At1g65010.1 68414.m07368 expressed protein similar to endosome-a... 28 4.5 At3g10200.1 68416.m01221 dehydration-responsive protein-related ... 28 6.0 At3g01370.1 68416.m00059 expressed protein contains Pfam domain,... 28 6.0 >At3g29375.1 68416.m03690 XH domain-containing protein contains Pfam profile: PF03469: XH domain Length = 335 Score = 30.7 bits (66), Expect = 0.85 Identities = 29/116 (25%), Positives = 51/116 (43%), Gaps = 6/116 (5%) Frame = +3 Query: 24 LKVISNELRKRVAVFLEQTEPLRAEMERKFHAINKLYEIIEYLRS--FEKVDDLSRQMKQ 197 +K + EL+KR+ V E+ E +E+E++ IN L Y E L QM+ Sbjct: 133 VKEENEELKKRLRVMKEELEDKDSELEQREGLINALLVKERYANDEILEAQKLLISQMRD 192 Query: 198 SSDDEQLVLL----YGELKHMCQLYQKGHRGNYVKEYTHYWHNVLKDKLTQHFDEV 353 +DD + + + +++ + ++ GN + Y W L+D Q F V Sbjct: 193 LTDDRTTIRVKRMGHLDVEPFVKASKRRLTGNDTEVYAE-WEENLRDPHWQPFKRV 247 >At5g46740.1 68418.m05758 ubiquitin-specific protease 21 (UBP21) identical to ubiquitin-specific protease 21 GI:11993482 [Arabidopsis thaliana] Length = 732 Score = 29.9 bits (64), Expect = 1.5 Identities = 19/61 (31%), Positives = 28/61 (45%), Gaps = 2/61 (3%) Frame = +3 Query: 174 DLSRQMKQSSDDEQLVL--LYGELKHMCQLYQKGHRGNYVKEYTHYWHNVLKDKLTQHFD 347 DLS M + D E LY ++H+ GH +YV+ WHN K+T+ + Sbjct: 394 DLSPFMSSNHDPEVSTRYHLYAFVEHIGIRATFGHYSSYVRSAPETWHNFDDSKVTRISE 453 Query: 348 E 350 E Sbjct: 454 E 454 >At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-containing protein / RhoGAP domain-containing protein weak similarity to rho-GTPase activating protein [Homo sapiens] GI:14245732; contains Pfam profiles PF00169: PH domain, PF00620: RhoGAP domain Length = 870 Score = 29.9 bits (64), Expect = 1.5 Identities = 14/42 (33%), Positives = 25/42 (59%) Frame = +3 Query: 3 NSMQIQNLKVISNELRKRVAVFLEQTEPLRAEMERKFHAINK 128 + + IQ L+ NELR+R+A L+A +ER+ A+++ Sbjct: 571 DELAIQRLETTKNELRQRIAKEARGNAILQASLERRKQALHE 612 >At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-containing protein / RhoGAP domain-containing protein weak similarity to rho-GTPase activating protein [Homo sapiens] GI:14245732; contains Pfam profiles PF00169: PH domain, PF00620: RhoGAP domain Length = 822 Score = 29.9 bits (64), Expect = 1.5 Identities = 14/42 (33%), Positives = 25/42 (59%) Frame = +3 Query: 3 NSMQIQNLKVISNELRKRVAVFLEQTEPLRAEMERKFHAINK 128 + + IQ L+ NELR+R+A L+A +ER+ A+++ Sbjct: 571 DELAIQRLETTKNELRQRIAKEARGNAILQASLERRKQALHE 612 >At4g17895.1 68417.m02667 ubiquitin-specific protease 20, putative (UBP20) identical to ubiquitin-specific protease 20 GI:11993480 [Arabidopsis thaliana] Length = 695 Score = 29.5 bits (63), Expect = 2.0 Identities = 28/103 (27%), Positives = 47/103 (45%), Gaps = 4/103 (3%) Frame = +3 Query: 84 PLRAEMERKFHAINKLY--EIIEYLRSFEKVDDLSRQMKQSSDDEQLVL--LYGELKHMC 251 PL A K N LY +I ++++ ++D L M+ ++E LY ++H Sbjct: 370 PLVATFHLKRFKNNGLYMEKIYKHVKIPLEID-LQPYMRNIQENEVSTKYHLYALVEHFG 428 Query: 252 QLYQKGHRGNYVKEYTHYWHNVLKDKLTQHFDEVMKLLKWPFI 380 GH +YV+ WH+ K+T+ DE M L + +I Sbjct: 429 YSVAYGHYSSYVRSAPKIWHHFDDSKVTR-IDEDMVLSQDSYI 470 >At5g12150.1 68418.m01426 pleckstrin homology (PH) domain-containing protein / RhoGAP domain-containing protein weak similarity to glucocorticoid receptor DNA binding factor 1 [Canis familiaris] GI:23266717; contains Pfam profiles PF00169: PH domain, PF00620: RhoGAP domain Length = 827 Score = 28.3 bits (60), Expect = 4.5 Identities = 13/42 (30%), Positives = 27/42 (64%) Frame = +3 Query: 3 NSMQIQNLKVISNELRKRVAVFLEQTEPLRAEMERKFHAINK 128 + + IQ L+ + +ELR+R+A + L+A +ER+ A+++ Sbjct: 562 DELLIQRLEHMKDELRQRIAKEAKGNAALQASLERRKQALHE 603 >At1g65010.1 68414.m07368 expressed protein similar to endosome-associated protein (GI:1016368) [Homo sapiens]; similar to Centromeric protein E (CENP-E protein) (Swiss-Prot:Q02224) [Homo sapiens] Length = 1318 Score = 28.3 bits (60), Expect = 4.5 Identities = 23/78 (29%), Positives = 37/78 (47%), Gaps = 8/78 (10%) Frame = +3 Query: 3 NSMQIQNLKVISNELRKRVAVFLEQTEPLRAEMERKFHAINKLYEII---EYLRSFE--- 164 N +QN+ + ELR+R L++ E L E +KL ++ E LR E Sbjct: 822 NVTNLQNISEENKELRERETTLLKKAEELSELNESLVDKASKLQTVVQENEELRERETAY 881 Query: 165 --KVDDLSRQMKQSSDDE 212 K+++LS+ + SD E Sbjct: 882 LKKIEELSKLHEILSDQE 899 >At3g10200.1 68416.m01221 dehydration-responsive protein-related similar to early-responsive to dehydration stress ERD3 protein [Arabidopsis thaliana] GI:15320410; contains Pfam profile PF03141: Putative methyltransferase Length = 591 Score = 27.9 bits (59), Expect = 6.0 Identities = 11/23 (47%), Positives = 13/23 (56%) Frame = +2 Query: 371 AFYKWS*TFPTSQRSYD*VHKSH 439 AF+ W F T R+YD VH H Sbjct: 487 AFHDWCEAFSTYPRTYDLVHSDH 509 >At3g01370.1 68416.m00059 expressed protein contains Pfam domain, PF04581: Protein of unknown function (DUF578) Length = 1011 Score = 27.9 bits (59), Expect = 6.0 Identities = 16/38 (42%), Positives = 22/38 (57%) Frame = -3 Query: 564 SSLTALREHEQANMGLVLDQNLGQQQHLCLSLQVLQSK 451 S ++ALRE + N GLV D L ++ L L Q L+ K Sbjct: 856 SQISALRERKSENDGLVTD--LSNRERLILRKQALKMK 891 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,921,076 Number of Sequences: 28952 Number of extensions: 247525 Number of successful extensions: 769 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 746 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 769 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1305036432 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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