BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner10g16f
(635 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g29375.1 68416.m03690 XH domain-containing protein contains P... 31 0.85
At5g46740.1 68418.m05758 ubiquitin-specific protease 21 (UBP21) ... 30 1.5
At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-contain... 30 1.5
At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-contain... 30 1.5
At4g17895.1 68417.m02667 ubiquitin-specific protease 20, putativ... 29 2.0
At5g12150.1 68418.m01426 pleckstrin homology (PH) domain-contain... 28 4.5
At1g65010.1 68414.m07368 expressed protein similar to endosome-a... 28 4.5
At3g10200.1 68416.m01221 dehydration-responsive protein-related ... 28 6.0
At3g01370.1 68416.m00059 expressed protein contains Pfam domain,... 28 6.0
>At3g29375.1 68416.m03690 XH domain-containing protein contains Pfam
profile: PF03469: XH domain
Length = 335
Score = 30.7 bits (66), Expect = 0.85
Identities = 29/116 (25%), Positives = 51/116 (43%), Gaps = 6/116 (5%)
Frame = +3
Query: 24 LKVISNELRKRVAVFLEQTEPLRAEMERKFHAINKLYEIIEYLRS--FEKVDDLSRQMKQ 197
+K + EL+KR+ V E+ E +E+E++ IN L Y E L QM+
Sbjct: 133 VKEENEELKKRLRVMKEELEDKDSELEQREGLINALLVKERYANDEILEAQKLLISQMRD 192
Query: 198 SSDDEQLVLL----YGELKHMCQLYQKGHRGNYVKEYTHYWHNVLKDKLTQHFDEV 353
+DD + + + +++ + ++ GN + Y W L+D Q F V
Sbjct: 193 LTDDRTTIRVKRMGHLDVEPFVKASKRRLTGNDTEVYAE-WEENLRDPHWQPFKRV 247
>At5g46740.1 68418.m05758 ubiquitin-specific protease 21 (UBP21)
identical to ubiquitin-specific protease 21 GI:11993482
[Arabidopsis thaliana]
Length = 732
Score = 29.9 bits (64), Expect = 1.5
Identities = 19/61 (31%), Positives = 28/61 (45%), Gaps = 2/61 (3%)
Frame = +3
Query: 174 DLSRQMKQSSDDEQLVL--LYGELKHMCQLYQKGHRGNYVKEYTHYWHNVLKDKLTQHFD 347
DLS M + D E LY ++H+ GH +YV+ WHN K+T+ +
Sbjct: 394 DLSPFMSSNHDPEVSTRYHLYAFVEHIGIRATFGHYSSYVRSAPETWHNFDDSKVTRISE 453
Query: 348 E 350
E
Sbjct: 454 E 454
>At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-containing
protein / RhoGAP domain-containing protein weak
similarity to rho-GTPase activating protein [Homo
sapiens] GI:14245732; contains Pfam profiles PF00169: PH
domain, PF00620: RhoGAP domain
Length = 870
Score = 29.9 bits (64), Expect = 1.5
Identities = 14/42 (33%), Positives = 25/42 (59%)
Frame = +3
Query: 3 NSMQIQNLKVISNELRKRVAVFLEQTEPLRAEMERKFHAINK 128
+ + IQ L+ NELR+R+A L+A +ER+ A+++
Sbjct: 571 DELAIQRLETTKNELRQRIAKEARGNAILQASLERRKQALHE 612
>At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-containing
protein / RhoGAP domain-containing protein weak
similarity to rho-GTPase activating protein [Homo
sapiens] GI:14245732; contains Pfam profiles PF00169: PH
domain, PF00620: RhoGAP domain
Length = 822
Score = 29.9 bits (64), Expect = 1.5
Identities = 14/42 (33%), Positives = 25/42 (59%)
Frame = +3
Query: 3 NSMQIQNLKVISNELRKRVAVFLEQTEPLRAEMERKFHAINK 128
+ + IQ L+ NELR+R+A L+A +ER+ A+++
Sbjct: 571 DELAIQRLETTKNELRQRIAKEARGNAILQASLERRKQALHE 612
>At4g17895.1 68417.m02667 ubiquitin-specific protease 20, putative
(UBP20) identical to ubiquitin-specific protease 20
GI:11993480 [Arabidopsis thaliana]
Length = 695
Score = 29.5 bits (63), Expect = 2.0
Identities = 28/103 (27%), Positives = 47/103 (45%), Gaps = 4/103 (3%)
Frame = +3
Query: 84 PLRAEMERKFHAINKLY--EIIEYLRSFEKVDDLSRQMKQSSDDEQLVL--LYGELKHMC 251
PL A K N LY +I ++++ ++D L M+ ++E LY ++H
Sbjct: 370 PLVATFHLKRFKNNGLYMEKIYKHVKIPLEID-LQPYMRNIQENEVSTKYHLYALVEHFG 428
Query: 252 QLYQKGHRGNYVKEYTHYWHNVLKDKLTQHFDEVMKLLKWPFI 380
GH +YV+ WH+ K+T+ DE M L + +I
Sbjct: 429 YSVAYGHYSSYVRSAPKIWHHFDDSKVTR-IDEDMVLSQDSYI 470
>At5g12150.1 68418.m01426 pleckstrin homology (PH) domain-containing
protein / RhoGAP domain-containing protein weak
similarity to glucocorticoid receptor DNA binding factor
1 [Canis familiaris] GI:23266717; contains Pfam profiles
PF00169: PH domain, PF00620: RhoGAP domain
Length = 827
Score = 28.3 bits (60), Expect = 4.5
Identities = 13/42 (30%), Positives = 27/42 (64%)
Frame = +3
Query: 3 NSMQIQNLKVISNELRKRVAVFLEQTEPLRAEMERKFHAINK 128
+ + IQ L+ + +ELR+R+A + L+A +ER+ A+++
Sbjct: 562 DELLIQRLEHMKDELRQRIAKEAKGNAALQASLERRKQALHE 603
>At1g65010.1 68414.m07368 expressed protein similar to
endosome-associated protein (GI:1016368) [Homo sapiens];
similar to Centromeric protein E (CENP-E protein)
(Swiss-Prot:Q02224) [Homo sapiens]
Length = 1318
Score = 28.3 bits (60), Expect = 4.5
Identities = 23/78 (29%), Positives = 37/78 (47%), Gaps = 8/78 (10%)
Frame = +3
Query: 3 NSMQIQNLKVISNELRKRVAVFLEQTEPLRAEMERKFHAINKLYEII---EYLRSFE--- 164
N +QN+ + ELR+R L++ E L E +KL ++ E LR E
Sbjct: 822 NVTNLQNISEENKELRERETTLLKKAEELSELNESLVDKASKLQTVVQENEELRERETAY 881
Query: 165 --KVDDLSRQMKQSSDDE 212
K+++LS+ + SD E
Sbjct: 882 LKKIEELSKLHEILSDQE 899
>At3g10200.1 68416.m01221 dehydration-responsive protein-related
similar to early-responsive to dehydration stress ERD3
protein [Arabidopsis thaliana] GI:15320410; contains
Pfam profile PF03141: Putative methyltransferase
Length = 591
Score = 27.9 bits (59), Expect = 6.0
Identities = 11/23 (47%), Positives = 13/23 (56%)
Frame = +2
Query: 371 AFYKWS*TFPTSQRSYD*VHKSH 439
AF+ W F T R+YD VH H
Sbjct: 487 AFHDWCEAFSTYPRTYDLVHSDH 509
>At3g01370.1 68416.m00059 expressed protein contains Pfam domain,
PF04581: Protein of unknown function (DUF578)
Length = 1011
Score = 27.9 bits (59), Expect = 6.0
Identities = 16/38 (42%), Positives = 22/38 (57%)
Frame = -3
Query: 564 SSLTALREHEQANMGLVLDQNLGQQQHLCLSLQVLQSK 451
S ++ALRE + N GLV D L ++ L L Q L+ K
Sbjct: 856 SQISALRERKSENDGLVTD--LSNRERLILRKQALKMK 891
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,921,076
Number of Sequences: 28952
Number of extensions: 247525
Number of successful extensions: 769
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 746
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 769
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1305036432
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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