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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fner10g16f
         (635 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g29375.1 68416.m03690 XH domain-containing protein contains P...    31   0.85 
At5g46740.1 68418.m05758 ubiquitin-specific protease 21 (UBP21) ...    30   1.5  
At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-contain...    30   1.5  
At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-contain...    30   1.5  
At4g17895.1 68417.m02667 ubiquitin-specific protease 20, putativ...    29   2.0  
At5g12150.1 68418.m01426 pleckstrin homology (PH) domain-contain...    28   4.5  
At1g65010.1 68414.m07368 expressed protein similar to endosome-a...    28   4.5  
At3g10200.1 68416.m01221 dehydration-responsive protein-related ...    28   6.0  
At3g01370.1 68416.m00059 expressed protein contains Pfam domain,...    28   6.0  

>At3g29375.1 68416.m03690 XH domain-containing protein contains Pfam
           profile: PF03469: XH domain
          Length = 335

 Score = 30.7 bits (66), Expect = 0.85
 Identities = 29/116 (25%), Positives = 51/116 (43%), Gaps = 6/116 (5%)
 Frame = +3

Query: 24  LKVISNELRKRVAVFLEQTEPLRAEMERKFHAINKLYEIIEYLRS--FEKVDDLSRQMKQ 197
           +K  + EL+KR+ V  E+ E   +E+E++   IN L     Y      E    L  QM+ 
Sbjct: 133 VKEENEELKKRLRVMKEELEDKDSELEQREGLINALLVKERYANDEILEAQKLLISQMRD 192

Query: 198 SSDDEQLVLL----YGELKHMCQLYQKGHRGNYVKEYTHYWHNVLKDKLTQHFDEV 353
            +DD   + +    + +++   +  ++   GN  + Y   W   L+D   Q F  V
Sbjct: 193 LTDDRTTIRVKRMGHLDVEPFVKASKRRLTGNDTEVYAE-WEENLRDPHWQPFKRV 247


>At5g46740.1 68418.m05758 ubiquitin-specific protease 21 (UBP21)
           identical to ubiquitin-specific protease 21 GI:11993482
           [Arabidopsis thaliana]
          Length = 732

 Score = 29.9 bits (64), Expect = 1.5
 Identities = 19/61 (31%), Positives = 28/61 (45%), Gaps = 2/61 (3%)
 Frame = +3

Query: 174 DLSRQMKQSSDDEQLVL--LYGELKHMCQLYQKGHRGNYVKEYTHYWHNVLKDKLTQHFD 347
           DLS  M  + D E      LY  ++H+      GH  +YV+     WHN    K+T+  +
Sbjct: 394 DLSPFMSSNHDPEVSTRYHLYAFVEHIGIRATFGHYSSYVRSAPETWHNFDDSKVTRISE 453

Query: 348 E 350
           E
Sbjct: 454 E 454


>At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-containing
           protein / RhoGAP domain-containing protein weak
           similarity to rho-GTPase activating protein [Homo
           sapiens] GI:14245732; contains Pfam profiles PF00169: PH
           domain, PF00620: RhoGAP domain
          Length = 870

 Score = 29.9 bits (64), Expect = 1.5
 Identities = 14/42 (33%), Positives = 25/42 (59%)
 Frame = +3

Query: 3   NSMQIQNLKVISNELRKRVAVFLEQTEPLRAEMERKFHAINK 128
           + + IQ L+   NELR+R+A        L+A +ER+  A+++
Sbjct: 571 DELAIQRLETTKNELRQRIAKEARGNAILQASLERRKQALHE 612


>At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-containing
           protein / RhoGAP domain-containing protein weak
           similarity to rho-GTPase activating protein [Homo
           sapiens] GI:14245732; contains Pfam profiles PF00169: PH
           domain, PF00620: RhoGAP domain
          Length = 822

 Score = 29.9 bits (64), Expect = 1.5
 Identities = 14/42 (33%), Positives = 25/42 (59%)
 Frame = +3

Query: 3   NSMQIQNLKVISNELRKRVAVFLEQTEPLRAEMERKFHAINK 128
           + + IQ L+   NELR+R+A        L+A +ER+  A+++
Sbjct: 571 DELAIQRLETTKNELRQRIAKEARGNAILQASLERRKQALHE 612


>At4g17895.1 68417.m02667 ubiquitin-specific protease 20, putative
           (UBP20) identical to ubiquitin-specific protease 20
           GI:11993480 [Arabidopsis thaliana]
          Length = 695

 Score = 29.5 bits (63), Expect = 2.0
 Identities = 28/103 (27%), Positives = 47/103 (45%), Gaps = 4/103 (3%)
 Frame = +3

Query: 84  PLRAEMERKFHAINKLY--EIIEYLRSFEKVDDLSRQMKQSSDDEQLVL--LYGELKHMC 251
           PL A    K    N LY  +I ++++   ++D L   M+   ++E      LY  ++H  
Sbjct: 370 PLVATFHLKRFKNNGLYMEKIYKHVKIPLEID-LQPYMRNIQENEVSTKYHLYALVEHFG 428

Query: 252 QLYQKGHRGNYVKEYTHYWHNVLKDKLTQHFDEVMKLLKWPFI 380
                GH  +YV+     WH+    K+T+  DE M L +  +I
Sbjct: 429 YSVAYGHYSSYVRSAPKIWHHFDDSKVTR-IDEDMVLSQDSYI 470


>At5g12150.1 68418.m01426 pleckstrin homology (PH) domain-containing
           protein / RhoGAP domain-containing protein weak
           similarity to glucocorticoid receptor DNA binding factor
           1 [Canis familiaris] GI:23266717; contains Pfam profiles
           PF00169: PH domain, PF00620: RhoGAP domain
          Length = 827

 Score = 28.3 bits (60), Expect = 4.5
 Identities = 13/42 (30%), Positives = 27/42 (64%)
 Frame = +3

Query: 3   NSMQIQNLKVISNELRKRVAVFLEQTEPLRAEMERKFHAINK 128
           + + IQ L+ + +ELR+R+A   +    L+A +ER+  A+++
Sbjct: 562 DELLIQRLEHMKDELRQRIAKEAKGNAALQASLERRKQALHE 603


>At1g65010.1 68414.m07368 expressed protein similar to
            endosome-associated protein (GI:1016368) [Homo sapiens];
            similar to Centromeric protein E (CENP-E protein)
            (Swiss-Prot:Q02224) [Homo sapiens]
          Length = 1318

 Score = 28.3 bits (60), Expect = 4.5
 Identities = 23/78 (29%), Positives = 37/78 (47%), Gaps = 8/78 (10%)
 Frame = +3

Query: 3    NSMQIQNLKVISNELRKRVAVFLEQTEPLRAEMERKFHAINKLYEII---EYLRSFE--- 164
            N   +QN+   + ELR+R    L++ E L    E      +KL  ++   E LR  E   
Sbjct: 822  NVTNLQNISEENKELRERETTLLKKAEELSELNESLVDKASKLQTVVQENEELRERETAY 881

Query: 165  --KVDDLSRQMKQSSDDE 212
              K+++LS+  +  SD E
Sbjct: 882  LKKIEELSKLHEILSDQE 899


>At3g10200.1 68416.m01221 dehydration-responsive protein-related
           similar to early-responsive to dehydration stress ERD3
           protein [Arabidopsis thaliana] GI:15320410; contains
           Pfam profile PF03141: Putative methyltransferase
          Length = 591

 Score = 27.9 bits (59), Expect = 6.0
 Identities = 11/23 (47%), Positives = 13/23 (56%)
 Frame = +2

Query: 371 AFYKWS*TFPTSQRSYD*VHKSH 439
           AF+ W   F T  R+YD VH  H
Sbjct: 487 AFHDWCEAFSTYPRTYDLVHSDH 509


>At3g01370.1 68416.m00059 expressed protein contains Pfam domain,
           PF04581: Protein of unknown function (DUF578)
          Length = 1011

 Score = 27.9 bits (59), Expect = 6.0
 Identities = 16/38 (42%), Positives = 22/38 (57%)
 Frame = -3

Query: 564 SSLTALREHEQANMGLVLDQNLGQQQHLCLSLQVLQSK 451
           S ++ALRE +  N GLV D  L  ++ L L  Q L+ K
Sbjct: 856 SQISALRERKSENDGLVTD--LSNRERLILRKQALKMK 891


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,921,076
Number of Sequences: 28952
Number of extensions: 247525
Number of successful extensions: 769
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 746
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 769
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1305036432
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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