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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fner10g10f
         (636 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g64370.1 68418.m08086 beta-ureidopropionase, putative / beta-...   194   3e-50
At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protei...    49   2e-06
At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protei...    49   2e-06
At5g67240.1 68418.m08475 exonuclease family protein contains exo...    29   2.6  
At5g22300.1 68418.m02601 nitrilase 4 (NIT4) identical to SP|P460...    29   3.4  
At5g06970.1 68418.m00789 expressed protein                             29   3.4  
At2g39980.1 68415.m04913 transferase family protein contains Pfa...    28   6.0  
At2g33010.1 68415.m04045 ubiquitin-associated (UBA)/TS-N domain-...    27   7.9  

>At5g64370.1 68418.m08086 beta-ureidopropionase, putative /
           beta-alanine synthase, putative similar to beta-alanine
           synthase [Dictyostelium discoideum] GI:14334061;
           contains Pfam profile PF00795: hydrolase,
           carbon-nitrogen family
          Length = 408

 Score =  194 bits (474), Expect = 3e-50
 Identities = 99/192 (51%), Positives = 128/192 (66%), Gaps = 3/192 (1%)
 Frame = +2

Query: 68  SLESIINNNLTGRDLEEFNRIHFGR---RNNLEIKLKESSIXXXXXXXXXXXXXXFPAKD 238
           SL  +++ NL     +E NR+  GR   R+  +I L ES+               F A  
Sbjct: 22  SLHQLLSANLKPELYQEVNRLLLGRNCGRSLEQIVLPESAKALSSKHDFDLQAASFSADK 81

Query: 239 EQTRPPRIVKVGIVQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNM 418
           EQ R PR+V+VG++Q+SIA+PT  P ++Q + IF+K+K IID AG  GVNI+C QE W M
Sbjct: 82  EQMRNPRVVRVGLIQNSIALPTTAPFSDQTRGIFDKLKPIIDAAGVAGVNILCLQEAWTM 141

Query: 419 PFAFCTREKQPWCEFAESAEDGPTTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVV 598
           PFAFCTRE++ WCEFAE   DG +T FL+ELA KY MVIVS ILERD  H ++LWNTAV+
Sbjct: 142 PFAFCTRERR-WCEFAEPV-DGESTKFLQELAKKYNMVIVSPILERDIDHGEVLWNTAVI 199

Query: 599 ISDTGNVIGKHR 634
           I + GN+IGKHR
Sbjct: 200 IGNNGNIIGKHR 211


>At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protein
           similar to Nit protein 2 [Homo sapiens] GI:9367116;
           contains Pfam profile PF00795: hydrolase,
           carbon-nitrogen family
          Length = 294

 Score = 49.2 bits (112), Expect = 2e-06
 Identities = 30/109 (27%), Positives = 52/109 (47%), Gaps = 2/109 (1%)
 Frame = +2

Query: 314 VNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQP-WCEFAESAED-GP 487
           V   KK   +  KK I+ A  +G  ++   E+WN P+   + +  P + E  ++  D  P
Sbjct: 97  VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPY---SNDSFPVYAEEIDAGGDASP 153

Query: 488 TTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHR 634
           +T  L E++ +  + I+   +   E+  D L+NT  V    G +  KHR
Sbjct: 154 STAMLSEVSKRLKITIIGGSI--PERVGDRLYNTCCVFGSDGELKAKHR 200


>At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protein
           similar to Nit protein 2 [Homo sapiens] GI:9367116;
           contains Pfam profile PF00795: hydrolase,
           carbon-nitrogen family
          Length = 369

 Score = 49.2 bits (112), Expect = 2e-06
 Identities = 30/109 (27%), Positives = 52/109 (47%), Gaps = 2/109 (1%)
 Frame = +2

Query: 314 VNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQP-WCEFAESAED-GP 487
           V   KK   +  KK I+ A  +G  ++   E+WN P+   + +  P + E  ++  D  P
Sbjct: 97  VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPY---SNDSFPVYAEEIDAGGDASP 153

Query: 488 TTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHR 634
           +T  L E++ +  + I+   +   E+  D L+NT  V    G +  KHR
Sbjct: 154 STAMLSEVSKRLKITIIGGSI--PERVGDRLYNTCCVFGSDGELKAKHR 200


>At5g67240.1 68418.m08475 exonuclease family protein contains
           exonuclease domain, Pfam:PF00929
          Length = 745

 Score = 29.1 bits (62), Expect = 2.6
 Identities = 12/29 (41%), Positives = 17/29 (58%)
 Frame = +1

Query: 490 HDLPSGTRHQVRNGDRVLDIRKGREAFGH 576
           HD+PS   H V NGD  LD++  +   G+
Sbjct: 441 HDVPSQELHGVLNGDFTLDVKPPKRKGGY 469


>At5g22300.1 68418.m02601 nitrilase 4 (NIT4) identical to SP|P46011
           Nitrilase 4 (EC 3.5.5.1) {Arabidopsis thaliana}
          Length = 355

 Score = 28.7 bits (61), Expect = 3.4
 Identities = 28/114 (24%), Positives = 50/114 (43%), Gaps = 13/114 (11%)
 Frame = +2

Query: 332 AIFNKVKKIIDVAGQEGVNIICFQELW--------NMPFAFCTREKQPWCEFAE---SAE 478
           A  +K ++++  A + G  ++ F E +            A  +R  +   +F +   SA 
Sbjct: 52  ATLDKAERLLSEAAENGSQLVVFPEAFIGGYPRGSTFELAIGSRTAKGRDDFRKYHASAI 111

Query: 479 D--GPTTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHR 634
           D  GP    L  +A KY + +V  ++ER+      L+ T +     G  +GKHR
Sbjct: 112 DVPGPEVERLALMAKKYKVYLVMGVIERE---GYTLYCTVLFFDSQGLFLGKHR 162


>At5g06970.1 68418.m00789 expressed protein
          Length = 1101

 Score = 28.7 bits (61), Expect = 3.4
 Identities = 13/59 (22%), Positives = 27/59 (45%)
 Frame = -1

Query: 633  RCFPITFPVSLITTAVFQSMSECFSSLSNIEDTITIAYLMASSRRKVVVGPSSADSANS 457
            R   I  P + +      ++    S LS +ED++ + ++    R K+V+  S  + + S
Sbjct: 834  RSINIDVPATAMLCVQLNTLHYAVSQLSKLEDSMWLRWIAKKPREKIVIRKSMVEKSKS 892


>At2g39980.1 68415.m04913 transferase family protein contains Pfam
           profile PF02458 transferase family
          Length = 482

 Score = 27.9 bits (59), Expect = 6.0
 Identities = 12/28 (42%), Positives = 14/28 (50%)
 Frame = -2

Query: 608 CH*LQPQCSKVCPNASRPFLISRTRSPL 525
           CH +Q  C   CPN   P LIS  +  L
Sbjct: 39  CHYIQKGCLFTCPNLPLPALISHLKHSL 66


>At2g33010.1 68415.m04045 ubiquitin-associated (UBA)/TS-N
           domain-containing protein contains Pfam profile PF00627:
           UBA/TS-N domain
          Length = 649

 Score = 27.5 bits (58), Expect = 7.9
 Identities = 17/38 (44%), Positives = 25/38 (65%), Gaps = 2/38 (5%)
 Frame = -1

Query: 609 VSLITTAVFQSMSECFSSLSNIEDT-ITIAYL-MASSR 502
           + L + + FQ+    FSSL+NI+ T  T+A L MASS+
Sbjct: 482 IGLPSQSQFQASGSSFSSLTNIDPTDFTVANLPMASSQ 519


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,957,441
Number of Sequences: 28952
Number of extensions: 292446
Number of successful extensions: 892
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 860
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 888
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1305036432
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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