BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fner10g09r (696 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 378 e-106 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 377 e-105 SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 163 3e-41 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 94 2e-20 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 32 0.068 SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 31 0.16 SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 29 0.64 SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 29 0.84 SPCC18.03 |||shuttle craft like transcriptional regulator|Schizo... 28 1.1 SPCC965.04c |||mitochondrial inner membrane i-AAA protease compl... 28 1.1 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 28 1.5 SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 26 4.5 SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch... 26 4.5 SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 5.9 SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 26 5.9 SPAC890.02c |alp7|mia1|TACC homolog |Schizosaccharomyces pombe|c... 26 5.9 SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po... 25 7.8 SPAC23E2.02 |lsd2|swm2, saf140|histone demethylase SWIRM2 |Schiz... 25 7.8 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 378 bits (931), Expect = e-106 Identities = 169/210 (80%), Positives = 190/210 (90%) Frame = -1 Query: 696 LIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVA 517 LI Q+VSSITASLRF G+LNVDL EFQTNLVPYPRIHFPLVTY+P++SA KA+HE SV Sbjct: 234 LIAQVVSSITASLRFAGSLNVDLNEFQTNLVPYPRIHFPLVTYSPIVSAAKAFHESNSVQ 293 Query: 516 EITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPT 337 EITN CFEP NQMVKCDPR G+YMA C+LYRGDV+P+DV AA+ +IK++RTIQFVDWCPT Sbjct: 294 EITNQCFEPYNQMVKCDPRTGRYMATCLLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPT 353 Query: 336 GFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYV 157 GFK+GI Y+PP VPG +AKV RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHWYV Sbjct: 354 GFKIGICYEPPQHVPGSGIAKVNRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYV 413 Query: 156 GEGMEEGEFSEAREDLAALEKDYEEVGMDS 67 GEGMEEGEFSEAREDLAALE+DYEEVG DS Sbjct: 414 GEGMEEGEFSEAREDLAALERDYEEVGQDS 443 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 377 bits (927), Expect = e-105 Identities = 169/210 (80%), Positives = 189/210 (90%) Frame = -1 Query: 696 LIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVA 517 LI Q+VSSITASLRF+G+LNVDL EFQTNLVPYPRIHFPLVTYAP++SA KA+HE SV Sbjct: 230 LIAQVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIHFPLVTYAPIVSAAKAFHESNSVQ 289 Query: 516 EITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPT 337 EITN CFEP NQMVKCDPR G+YMA C+LYRGDV+P+DV AA+ TIK KRTIQFVDWCPT Sbjct: 290 EITNQCFEPYNQMVKCDPRAGRYMATCLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPT 349 Query: 336 GFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYV 157 GFK+GI +PP + G ++AKV RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHWYV Sbjct: 350 GFKIGICDRPPQHIEGSEIAKVDRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYV 409 Query: 156 GEGMEEGEFSEAREDLAALEKDYEEVGMDS 67 GEGMEEGEFSEAREDLAALE+DYEEVG DS Sbjct: 410 GEGMEEGEFSEAREDLAALERDYEEVGQDS 439 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 163 bits (395), Expect = 3e-41 Identities = 79/212 (37%), Positives = 125/212 (58%), Gaps = 3/212 (1%) Frame = -1 Query: 696 LIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVA 517 L+ +++ +T S RF G LN DL + N+VP+PR+HF +V +AP+ + + + +SV Sbjct: 228 LVSAVMAGVTTSFRFPGELNSDLRKLAVNMVPFPRLHFFMVGFAPLAAIGSSSFQAVSVP 287 Query: 516 EITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPT 337 E+T F+ N MV DPRHG+Y+ L+RG V K+V+ I +++TK + FV+W P Sbjct: 288 ELTQQMFDANNMMVAADPRHGRYLTVAALFRGKVSMKEVDEQIRSVQTKNSAYFVEWIPD 347 Query: 336 GFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYV 157 + PP DL + + + N+T+I E + RL +F M+ ++AF+HWY Sbjct: 348 NVLKAVCSVPPK-----DL---KMSATFIGNSTSIQEIFRRLGDQFSAMFRRKAFLHWYT 399 Query: 156 GEGMEEGEFSEAR---EDLAALEKDYEEVGMD 70 GEGM+E EF+EA DL + + Y+E G+D Sbjct: 400 GEGMDEMEFTEAESNMNDLVSEYQQYQEAGID 431 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 93.9 bits (223), Expect = 2e-20 Identities = 57/211 (27%), Positives = 107/211 (50%), Gaps = 7/211 (3%) Frame = -1 Query: 696 LIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVIS---AEKAYHEQL 526 L+ ++S+ T +LR+ G +N DL +L+P PR HF L +Y P + E + Sbjct: 231 LVSTVMSASTTTLRYPGYMNNDLVSIIASLIPSPRCHFLLTSYTPFTNQQVEEAKAIRKT 290 Query: 525 SVAEITNACFEPANQMVKCDP-RHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVD 349 +V ++ P NQMV +P + +++ + +G+ P DV+ ++ I+ +R F+ Sbjct: 291 TVLDVMRRLLLPKNQMVSVNPSKKSCFISILDIIQGEADPADVHKSLLRIRERRYASFIP 350 Query: 348 WCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFV 169 W P +V ++ + P + ++ + ML+N T+IA + R ++D + + AF+ Sbjct: 351 WGPASIQVALSKKSPYIKTNHRVSGL-----MLANHTSIASLFKRTLDQYDRLRKRNAFL 405 Query: 168 HWYVGEGMEE---GEFSEAREDLAALEKDYE 85 Y E + E EF +R+ +A L +YE Sbjct: 406 EQYKKEAIFEDDLNEFDSSRDVVADLINEYE 436 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 32.3 bits (70), Expect = 0.068 Identities = 29/112 (25%), Positives = 48/112 (42%), Gaps = 2/112 (1%) Frame = -2 Query: 632 TSPSSRLTWCLTPVSTSHWSRTRQSSLPRRPTM--NSFPSPRSQTHASSPPTRW*NATPV 459 TS S L+ + +++ T S+ P PT+ S +P + T+ ++ + +TPV Sbjct: 381 TSSSPLLSTSTSCTTSTSIPPTGNSTTPVTPTVPPTSSSTPLTTTNCTTSTSVPYTSTPV 440 Query: 458 MASTWLAVCCTVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHP 303 ++ CT TS P T + S P +N +T S TS P Sbjct: 441 TSTPLATTNCTTSTSVPY--TSTPVTSTPLTTTNCTTSTSIPYTSTPVTSTP 490 Score = 30.3 bits (65), Expect = 0.28 Identities = 29/105 (27%), Positives = 50/105 (47%) Frame = -2 Query: 623 SSRLTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMASTW 444 S+ + + TPV++S++ T SS P T P + T+ ++ + +TPV ++ Sbjct: 499 STSVPYTSTPVTSSNY--TISSSTPVTST------PVTTTNCTTSTSVLYTSTPVTSTPL 550 Query: 443 LAVCCTVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTS 309 CT TS P T P S ++ S++ PV+ + TTS Sbjct: 551 ATTNCTTSTSVPYTST-PVTSSNYTISSSTPVTSTPVTTTNCTTS 594 Score = 28.3 bits (60), Expect = 1.1 Identities = 27/107 (25%), Positives = 48/107 (44%) Frame = -2 Query: 629 SPSSRLTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMAS 450 +P+ T TP++T++ T +S+P T + +P + T+ ++ + +TPV ++ Sbjct: 410 TPTVPPTSSSTPLTTTNC--TTSTSVPYTSTPVT-STPLATTNCTTSTSVPYTSTPVTST 466 Query: 449 TWLAVCCTVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTS 309 CT TS P T + S P +N +T S TS Sbjct: 467 PLTTTNCTTSTSIPY--TSTPVTSTPLTTTNCTTSTSVPYTSTPVTS 511 Score = 25.8 bits (54), Expect = 5.9 Identities = 27/98 (27%), Positives = 49/98 (50%), Gaps = 1/98 (1%) Frame = -2 Query: 599 TPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMASTWLAVCCTVV 420 TP++T++ T +S+P T + +P + T+ ++ + +TPV +S + T V Sbjct: 466 TPLTTTNC--TTSTSIPYTSTPVT-STPLTTTNCTTSTSVPYTSTPVTSSNYTISSSTPV 522 Query: 419 TSYPRM*TRPSLPSKPSVLSNSSTGVQ-PVSRSVSTTS 309 TS P T + + SVL S+ P++ + TTS Sbjct: 523 TSTPV--TTTNCTTSTSVLYTSTPVTSTPLATTNCTTS 558 >SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 857 Score = 31.1 bits (67), Expect = 0.16 Identities = 24/89 (26%), Positives = 33/89 (37%) Frame = -2 Query: 557 SLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMASTWLAVCCTVVTSYPRM*TRPSLPS 378 S+P+ P P S + P P + S VV P + RP++P Sbjct: 513 SVPQPPAAPVVPEAPSVHQPPAAPV-----APEVPSAPQRPAAPVVPEAPSVPQRPAVPV 567 Query: 377 KPSVLSNSSTGVQPVSRSVSTTSHPPWCP 291 P LS V PV+ V + PP P Sbjct: 568 VPEALSVPQPPVAPVAPEVPSVPQPPVAP 596 Score = 25.4 bits (53), Expect = 7.8 Identities = 23/90 (25%), Positives = 31/90 (34%) Frame = -2 Query: 557 SLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMASTWLAVCCTVVTSYPRM*TRPSLPS 378 S+P+RP + P S P P + +A S P+ P P Sbjct: 558 SVPQRPAVPVVPEALSVPQPPVAPVA--PEVPSVPQPPVAPVVPEAPSVPQPPVAPVAPE 615 Query: 377 KPSVLSNSSTGVQPVSRSVSTTSHPPWCPE 288 PSV + V P + SV P PE Sbjct: 616 VPSVPQRPAVPVVPEAPSVPQPPAAPVVPE 645 >SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 534 Score = 29.1 bits (62), Expect = 0.64 Identities = 23/81 (28%), Positives = 41/81 (50%) Frame = -2 Query: 590 STSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMASTWLAVCCTVVTSY 411 STS + T SS RP +S S +H SS ++ ++ PV ++ + + +S+ Sbjct: 377 STSSSTLTSSSSSSSRPASSSSHSSSLSSHKSSSSSKS-SSAPVSSAFYHNSTSSRSSSH 435 Query: 410 PRM*TRPSLPSKPSVLSNSST 348 + SL SKP + ++SS+ Sbjct: 436 SSSHSLSSLSSKPILTASSSS 456 >SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 3227 Score = 28.7 bits (61), Expect = 0.84 Identities = 20/73 (27%), Positives = 34/73 (46%) Frame = +1 Query: 385 SDGRVHILGYDVTTVQHTASHVLAMTGVAFHHLVGGLEACVCDLGDGKLFMVGLLGRDDW 564 S G +LGY ++ A++V+A + V HL+ G D + K G D Sbjct: 407 SAGLTSLLGYHLSVKTPQATYVVARSIVMLDHLIDGYSMAFPDFSESK-------GLDML 459 Query: 565 RVRDQWEVDTGVR 603 R Q+E++ G++ Sbjct: 460 VDRVQYELEAGLQ 472 >SPCC18.03 |||shuttle craft like transcriptional regulator|Schizosaccharomyces pombe|chr 3|||Manual Length = 1077 Score = 28.3 bits (60), Expect = 1.1 Identities = 14/42 (33%), Positives = 18/42 (42%) Frame = -1 Query: 513 ITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAI 388 + ACFEP N C H K C L + KD N ++ Sbjct: 361 LCGACFEPINAKCYCG-LHSKTYPCSSLPSPSISKKDENGSV 401 >SPCC965.04c |||mitochondrial inner membrane i-AAA protease complex subunit Yme1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 709 Score = 28.3 bits (60), Expect = 1.1 Identities = 21/66 (31%), Positives = 29/66 (43%), Gaps = 2/66 (3%) Frame = -2 Query: 221 GLALTTSSTSCTPSVLSCTGTSVRVWRRESSPKPVRTWLPS--RRITKKSAWTPLKARVR 48 G+ TS TS T S S++ S+P P W P+ S+ TP+ V Sbjct: 148 GVPKFTSDTSSTVSSTPSLNHSLQNSMPPSTPTPPPVWAPTIVSSALGTSSKTPVYVVVD 207 Query: 47 EPKSTK 30 EP+ TK Sbjct: 208 EPRFTK 213 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 27.9 bits (59), Expect = 1.5 Identities = 17/49 (34%), Positives = 26/49 (53%) Frame = -2 Query: 626 PSSRLTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTR 480 P+++ P S + T +SS+P P N+ PSP S + AS+ P R Sbjct: 1232 PTAKAPPVPAPSSEAPSVSTPRSSVPS-PHSNASPSPTSSSMASAAPAR 1279 >SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1158 Score = 26.2 bits (55), Expect = 4.5 Identities = 11/28 (39%), Positives = 17/28 (60%) Frame = -2 Query: 563 QSSLPRRPTMNSFPSPRSQTHASSPPTR 480 + SLPRRP+ +P S T ++ PP + Sbjct: 738 RGSLPRRPSSALLTNPISITKSNPPPVK 765 >SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 628 Score = 26.2 bits (55), Expect = 4.5 Identities = 14/32 (43%), Positives = 21/32 (65%), Gaps = 3/32 (9%) Frame = -2 Query: 389 SLPSKPSV---LSNSSTGVQPVSRSVSTTSHP 303 +LP KPS+ +++S V+P S STTS+P Sbjct: 5 TLPPKPSISPSIASSFPTVKPFSSQNSTTSNP 36 >SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 751 Score = 25.8 bits (54), Expect = 5.9 Identities = 16/47 (34%), Positives = 26/47 (55%) Frame = -2 Query: 629 SPSSRLTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSP 489 SPS LT L S++ S + + + R+ T +S SP+ Q+ S+P Sbjct: 619 SPSKMLT-TLRNNSSTFPSLRKNAMIARKSTADSLSSPKRQSVPSTP 664 >SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosaccharomyces pombe|chr 1|||Manual Length = 157 Score = 25.8 bits (54), Expect = 5.9 Identities = 10/20 (50%), Positives = 11/20 (55%) Frame = +3 Query: 408 WVRRHHGTAYSKPCTCHDGG 467 W R H Y+KPC DGG Sbjct: 16 WRRDHPFGFYAKPCKSSDGG 35 >SPAC890.02c |alp7|mia1|TACC homolog |Schizosaccharomyces pombe|chr 1|||Manual Length = 474 Score = 25.8 bits (54), Expect = 5.9 Identities = 16/51 (31%), Positives = 31/51 (60%) Frame = -2 Query: 602 LTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMAS 450 LTP + S +S + +S +R ++N+ PS + +H+SS T + PV+++ Sbjct: 130 LTPKNPSLFSSSNAAS--QRGSLNTAPSNFAYSHSSSLQTSASSRPPVLSN 178 >SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces pombe|chr 1|||Manual Length = 665 Score = 25.4 bits (53), Expect = 7.8 Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 2/34 (5%) Frame = -2 Query: 392 PSLPSKPSVLSNSST--GVQPVSRSVSTTSHPPW 297 P PS+P+++SN ST G+Q V V + W Sbjct: 531 PISPSRPALISNISTKKGIQVVGNMVYDPTRLRW 564 >SPAC23E2.02 |lsd2|swm2, saf140|histone demethylase SWIRM2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1273 Score = 25.4 bits (53), Expect = 7.8 Identities = 22/84 (26%), Positives = 36/84 (42%) Frame = -2 Query: 596 PVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMASTWLAVCCTVVT 417 P+ SH S + S+ R + + H S PT+ +AT + S AV T Sbjct: 253 PLDASHLSSQQLLSMYRDQVSHGVTPSTFRNHESFMPTQLVSATELSKSVDNAVLPIPPT 312 Query: 416 SYPRM*TRPSLPSKPSVLSNSSTG 345 + P + P S P + S +++G Sbjct: 313 TAPAV-VSPPASSFPLMSSAATSG 335 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,958,899 Number of Sequences: 5004 Number of extensions: 65701 Number of successful extensions: 261 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 236 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 259 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 321151040 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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