BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fner10g09f (652 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 283 1e-77 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 276 2e-75 SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 144 1e-35 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 123 2e-29 SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 5.4 SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 5.4 SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 7.2 SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2 |Schizos... 25 9.5 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 283 bits (695), Expect = 1e-77 Identities = 125/184 (67%), Positives = 150/184 (81%) Frame = +2 Query: 101 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 280 MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60 Query: 281 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 460 +VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120 Query: 461 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTX 640 +IR++AD C+GLQGFL+FH LL+ERL+++Y KKSKL+F++YPAPQVST Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180 Query: 641 VVEP 652 VVEP Sbjct: 181 VVEP 184 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 276 bits (677), Expect = 2e-75 Identities = 126/189 (66%), Positives = 152/189 (80%), Gaps = 5/189 (2%) Frame = +2 Query: 101 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 265 MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59 Query: 266 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 445 TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++ Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119 Query: 446 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAP 625 D VL+RIR++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++YPAP Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179 Query: 626 QVSTXVVEP 652 QVST VVEP Sbjct: 180 QVSTSVVEP 188 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 144 bits (348), Expect = 1e-35 Identities = 71/184 (38%), Positives = 101/184 (54%) Frame = +2 Query: 101 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 280 MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58 Query: 281 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 460 +VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118 Query: 461 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTX 640 +R+ A+ C LQGF + H LL+ ++ +Y + F++ PAP+ S Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178 Query: 641 VVEP 652 VVEP Sbjct: 179 VVEP 182 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 123 bits (297), Expect = 2e-29 Identities = 68/186 (36%), Positives = 105/186 (56%), Gaps = 3/186 (1%) Frame = +2 Query: 104 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 283 RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++ Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60 Query: 284 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 457 +PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++ Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119 Query: 458 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VS 634 D I + AD L+GF + H L+ERL+ Y KK ++++P Q VS Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179 Query: 635 TXVVEP 652 VV+P Sbjct: 180 DVVVQP 185 >SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 534 Score = 25.8 bits (54), Expect = 5.4 Identities = 15/35 (42%), Positives = 19/35 (54%) Frame = +3 Query: 180 STASSLMARCPQTRPSGVETILSTLSSARPELAST 284 ST SSL + ++PS T ST SSA P S+ Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207 >SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1275 Score = 25.8 bits (54), Expect = 5.4 Identities = 13/22 (59%), Positives = 13/22 (59%) Frame = +1 Query: 199 WPDAHRQDHRGWRRFFQHFLQR 264 W A R D R R FQHFLQR Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611 >SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pombe|chr 3|||Manual Length = 534 Score = 25.4 bits (53), Expect = 7.2 Identities = 15/41 (36%), Positives = 21/41 (51%) Frame = +3 Query: 90 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 212 L+S +L+ P SR++ P S + STASSL P Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210 >SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 400 Score = 25.0 bits (52), Expect = 9.5 Identities = 10/23 (43%), Positives = 14/23 (60%) Frame = -1 Query: 154 ITDLDSGLANVYRDALTHFDLSF 86 +T +D GLA YRD TH + + Sbjct: 146 VTMIDFGLAKKYRDFKTHVHIPY 168 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,765,395 Number of Sequences: 5004 Number of extensions: 58603 Number of successful extensions: 204 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 159 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 200 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 293780908 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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