Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner10g05f
(615 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g05170.1 68415.m00544 vacuolar protein sorting 11 family prot... 31 0.61
At5g09400.1 68418.m01089 potassium transporter family protein si... 29 2.4
At1g77240.1 68414.m08996 AMP-binding protein, putative strong si... 29 2.4
At5g49160.1 68418.m06085 DNA (cytosine-5-)-methyltransferase (AT... 29 3.2
At4g13290.1 68417.m02078 cytochrome P450 71A19, putative (CYP71A... 29 3.2
At1g62250.2 68414.m07023 expressed protein 29 3.2
At1g62250.1 68414.m07022 expressed protein 29 3.2
At1g47310.1 68414.m05238 expressed protein 28 4.3
At3g62000.1 68416.m06963 O-methyltransferase family 3 protein se... 28 5.7
At3g12775.1 68416.m01595 ubiquitin-conjugating enzyme family pro... 28 5.7
At3g04160.1 68416.m00440 expressed protein ; expression supporte... 28 5.7
At2g25290.1 68415.m03025 octicosapeptide/Phox/Bem1p (PB1) domain... 28 5.7
At1g64150.1 68414.m07267 expressed protein contains Pfam profile... 28 5.7
At5g53950.1 68418.m06712 no apical meristem (NAM) family protein... 27 7.5
At4g32070.1 68417.m04564 octicosapeptide/Phox/Bem1p (PB1) domain... 27 7.5
At1g56660.1 68414.m06516 expressed protein 27 7.5
At3g47730.1 68416.m05200 ABC transporter family protein AbcA, Di... 27 9.9
At3g15380.1 68416.m01950 choline transporter-related contains we... 27 9.9
At2g36390.1 68415.m04466 1,4-alpha-glucan branching enzyme / sta... 27 9.9
At2g17033.2 68415.m01965 pentatricopeptide (PPR) repeat-containi... 27 9.9
At2g17033.1 68415.m01964 pentatricopeptide (PPR) repeat-containi... 27 9.9
>At2g05170.1 68415.m00544 vacuolar protein sorting 11 family protein
/ VPS11 family protein similar to Vacuolar protein
sorting 11 (hVPS11) (PP3476) (Swiss-Prot:Q9H270) [Homo
sapiens]; similar to Vacuolar biogenesis protein END1
(PEP5 protein) (Vacuolar protein sorting 11)
(Swiss-Prot:P12868) [Saccharomyces cerevisiae]
Length = 932
Score = 31.1 bits (67), Expect = 0.61
Identities = 21/85 (24%), Positives = 40/85 (47%), Gaps = 4/85 (4%)
Frame = +2
Query: 155 IGEYETAIAKCSEYLKEKKGEVIKEAVKRLIENGKRNTMDFAYQLWTKDG--KEIVKSYF 328
+G Y+ A+ S + G I++ K LIE+ + T+D +L T+ G + S
Sbjct: 498 LGNYDEALQYVSSLEPSQAGVTIEQYGKILIEHKPKETIDILMRLCTEQGIPNGVFLSML 557
Query: 329 --PIQFRVIFTEQTVKLINKRDHHA 397
P+ F +F + L++ + +A
Sbjct: 558 PSPVDFITVFVQHPHSLMHFLERYA 582
>At5g09400.1 68418.m01089 potassium transporter family protein
similar to K+ transporter HAK5 [Arabidopsis thaliana]
GI:7108597; contains Pfam profile PF02705: K+ potassium
transporter; KUP/HAK/KT Transporter family member,
PMID:11500563; Note: possible sequencing error causes a
frameshift in the 4th exon|15810448|gb|AY056263
Length = 858
Score = 29.1 bits (62), Expect = 2.4
Identities = 19/73 (26%), Positives = 35/73 (47%)
Frame = -1
Query: 237 FTASLITSPFFSFRYSEHLAIAVSYSPMTTLIYSCSASTSSVLGASVALEASAHTARTKA 58
F +S+ S F+ + ++A ++ MTT +SC S+ LG L+ HT+R
Sbjct: 411 FFSSVPGSAFWPVLFIANIAALIASRTMTTATFSC-IKQSTALGCFPRLKI-IHTSRKFM 468
Query: 57 NKVSLILAQWLSL 19
++ + + W L
Sbjct: 469 GQIYIPVLNWFLL 481
>At1g77240.1 68414.m08996 AMP-binding protein, putative strong
similarity to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501
Length = 545
Score = 29.1 bits (62), Expect = 2.4
Identities = 19/67 (28%), Positives = 32/67 (47%), Gaps = 1/67 (1%)
Frame = +2
Query: 314 VKSYFPIQFRVIFTEQTVKLINKR-DHHALKLIDQQNHNKIAFGDSKDKTSKKVSWKFTP 490
V S + +QF V + + IN R D HAL ++ + + +K+ F D + + F P
Sbjct: 76 VPSVYELQFAVPMSGAVLNNINPRLDAHALSVLLRHSESKLVFVDHHSSSLVLEAVSFLP 135
Query: 491 VLENNRV 511
E R+
Sbjct: 136 KDERPRL 142
>At5g49160.1 68418.m06085 DNA (cytosine-5-)-methyltransferase
(ATHIM) identical to SP|P34881 DNA
(cytosine-5)-methyltransferase AthI (EC 2.1.1.37)
{Arabidopsis thaliana}
Length = 1534
Score = 28.7 bits (61), Expect = 3.2
Identities = 11/23 (47%), Positives = 15/23 (65%)
Frame = -2
Query: 299 PLSITGRRSPWCSSCRFRSDASR 231
PLS GR S +C+SC+ R D +
Sbjct: 869 PLSDIGRSSGFCTSCKIREDEEK 891
>At4g13290.1 68417.m02078 cytochrome P450 71A19, putative (CYP71A19)
Identical to Cytochrome P450 (SP:Q9T0K0) [Arabidopsis
thaliana]; similar to cytochrome P450LXXIA1, Persea
americana, M32885
Length = 490
Score = 28.7 bits (61), Expect = 3.2
Identities = 11/31 (35%), Positives = 19/31 (61%)
Frame = -3
Query: 397 GVMVPFVYELDSLLGEDHSKLDGEVRFDDFL 305
G +P + +D + G+DH + + RFD+FL
Sbjct: 221 GEYIPSLSWIDKIRGQDHKMEEVDKRFDEFL 251
>At1g62250.2 68414.m07023 expressed protein
Length = 223
Score = 28.7 bits (61), Expect = 3.2
Identities = 11/43 (25%), Positives = 23/43 (53%)
Frame = +3
Query: 51 LCLPSSSPCVRWLLTPHLHQELMTYWQSSCI*VSSLVNTRPLS 179
LC P + +RW TP + E+++ W+ C +++ R ++
Sbjct: 181 LCTPQPT-VIRWSSTPSVSDEILSKWRGFCAVIANAYYIRGMA 222
>At1g62250.1 68414.m07022 expressed protein
Length = 267
Score = 28.7 bits (61), Expect = 3.2
Identities = 11/43 (25%), Positives = 23/43 (53%)
Frame = +3
Query: 51 LCLPSSSPCVRWLLTPHLHQELMTYWQSSCI*VSSLVNTRPLS 179
LC P + +RW TP + E+++ W+ C +++ R ++
Sbjct: 181 LCTPQPT-VIRWSSTPSVSDEILSKWRGFCAVIANAYYIRGMA 222
>At1g47310.1 68414.m05238 expressed protein
Length = 395
Score = 28.3 bits (60), Expect = 4.3
Identities = 19/67 (28%), Positives = 35/67 (52%)
Frame = +2
Query: 353 TEQTVKLINKRDHHALKLIDQQNHNKIAFGDSKDKTSKKVSWKFTPVLENNRVYFKIMST 532
T QTV + + A ++ Q + + G ++ SKKV+W+ P +E RV+F++ +
Sbjct: 286 TRQTVSSVTAK-LKASGMVRFQLEIERSIGKNESVISKKVAWRTKPKIE--RVWFEVTAK 342
Query: 533 EDKQYLK 553
+ LK
Sbjct: 343 IEGDKLK 349
>At3g62000.1 68416.m06963 O-methyltransferase family 3 protein
several O-methyltransferases - different species;
contains Pfam 01596 O-methyltransferase domain
Length = 278
Score = 27.9 bits (59), Expect = 5.7
Identities = 26/126 (20%), Positives = 56/126 (44%), Gaps = 2/126 (1%)
Frame = +2
Query: 122 VLAEQLYMSVVIGEYETAIAKCSEYLKEKKGEVIKEAVKRLIENGKRNTMDFAYQLWTKD 301
V E+ S+ + + +A S + K+G + E++K +I+NG+ + DFA+
Sbjct: 147 VACERDSNSLEVAKRYYELAGVSHKVNVKQG-LAAESLKSMIQNGEGASYDFAF---VDA 202
Query: 302 GKEIVKSYFPIQFRVIFTEQTVKLINKRDHHALK--LIDQQNHNKIAFGDSKDKTSKKVS 475
K + + YF + +++ + + N H + +++ I + K K+VS
Sbjct: 203 DKRMYQDYFELLLQLVRVGGVIVMDNVLWHGRVSDPMVNDAKTISIRNFNKKLMDDKRVS 262
Query: 476 WKFTPV 493
P+
Sbjct: 263 ISMVPI 268
>At3g12775.1 68416.m01595 ubiquitin-conjugating enzyme family
protein contains Pfam profile PF00179:
Ubiquitin-conjugating enzyme
Length = 362
Score = 27.9 bits (59), Expect = 5.7
Identities = 16/58 (27%), Positives = 31/58 (53%), Gaps = 1/58 (1%)
Frame = +2
Query: 443 DSKDKTSKKVSWKFTPVLENNRVYF-KIMSTEDKQYLKLDNTKGSSDDRIIYGDSTAD 613
D +++ KK+ WK E N+VY +I++++ K L+ K + YG S+++
Sbjct: 277 DMEEERDKKMFWKMYFAFEGNKVYCEQILNSDLKAELEKLKEKEYTLSDYYYGSSSSN 334
>At3g04160.1 68416.m00440 expressed protein ; expression supported
by MPSS
Length = 712
Score = 27.9 bits (59), Expect = 5.7
Identities = 11/37 (29%), Positives = 18/37 (48%)
Frame = +1
Query: 229 SREASDRKRQEEHHGLRLPVMDKGWKGNRQILLPHPV 339
SR ++E G +P DK WKG + + +P+
Sbjct: 563 SRSPIGNDQKESDFGYSIPSTDKQWKGENRADIEYPI 599
>At2g25290.1 68415.m03025 octicosapeptide/Phox/Bem1p (PB1)
domain-containing protein / tetratricopeptide repeat
(TPR)-containing protein low similarity to SP|Q99614
Tetratricopeptide repeat protein 1 {Homo sapiens};
contains Pfam profiles PF00564: PB1 domain, PF00515: TPR
Domain
Length = 697
Score = 27.9 bits (59), Expect = 5.7
Identities = 17/49 (34%), Positives = 25/49 (51%)
Frame = +2
Query: 140 YMSVVIGEYETAIAKCSEYLKEKKGEVIKEAVKRLIENGKRNTMDFAYQ 286
YM + +GEY AI +C+ L E K +KR N +DFA++
Sbjct: 100 YMQMGLGEYPNAINECNLAL-EASPRFSKALLKRARCYEALNKLDFAFR 147
>At1g64150.1 68414.m07267 expressed protein contains Pfam profile
PF01169: Uncharacterized protein family UPF0016
Length = 370
Score = 27.9 bits (59), Expect = 5.7
Identities = 12/32 (37%), Positives = 19/32 (59%)
Frame = -3
Query: 607 GAITVDDTVITRTFRVIELQVLFVFGGHDLEV 512
G +T+ V+ RTF ++ + F FGG DL +
Sbjct: 200 GIMTIISVVLGRTFHYVDEVLPFRFGGTDLPI 231
>At5g53950.1 68418.m06712 no apical meristem (NAM) family protein
identical to no apical meristem protein CUC2
(GI:1944132) [Arabidopsis thaliana]; contains Pfam
PF02365: No apical meristem (NAM) domain;
Length = 375
Score = 27.5 bits (58), Expect = 7.5
Identities = 18/56 (32%), Positives = 25/56 (44%), Gaps = 3/56 (5%)
Frame = +3
Query: 456 KPARKSPGSLPPCWKTTE---YTSRSCPPKTNST*SSITRKVLVMTVSSTVIAPLT 614
+P+ S SLPP T YT SC + ST +++T + VS P T
Sbjct: 214 QPSSPSSVSLPPLLDPTTTLGYTDSSCSYDSRSTNTTVTASAITEHVSCFSTVPTT 269
>At4g32070.1 68417.m04564 octicosapeptide/Phox/Bem1p (PB1)
domain-containing protein / tetratricopeptide repeat
(TPR)-containing protein similar to SP|Q99614
Tetratricopeptide repeat protein 1 {Homo sapiens};
contains Pfam profiles PF00564: PB1 domain, PF00515: TPR
Domain
Length = 811
Score = 27.5 bits (58), Expect = 7.5
Identities = 16/49 (32%), Positives = 26/49 (53%)
Frame = +2
Query: 140 YMSVVIGEYETAIAKCSEYLKEKKGEVIKEAVKRLIENGKRNTMDFAYQ 286
YM + +GEY AI++C+ L E K V+R N +D+A++
Sbjct: 99 YMQMGLGEYPNAISECNLAL-EASPRYSKALVRRSRCYEALNKLDYAFR 146
>At1g56660.1 68414.m06516 expressed protein
Length = 522
Score = 27.5 bits (58), Expect = 7.5
Identities = 16/74 (21%), Positives = 34/74 (45%)
Frame = +1
Query: 199 EGKEGRGYQGSREASDRKRQEEHHGLRLPVMDKGWKGNRQILLPHPV*SDLHRADCQAHK 378
+GK+ +G +G E D ++++EH + +K K N++ + + + K
Sbjct: 213 KGKKEKGEKGDLEKEDEEKKKEHDETDQEMKEKDSKKNKKKEKDESCAEEKKKKPDKEKK 272
Query: 379 QKGPSRPQVDRPTK 420
+K S + D+ K
Sbjct: 273 EKDESTEKEDKKLK 286
>At3g47730.1 68416.m05200 ABC transporter family protein AbcA,
Dictyostelium discoideum, DDU66526
Length = 983
Score = 27.1 bits (57), Expect = 9.9
Identities = 15/53 (28%), Positives = 25/53 (47%), Gaps = 2/53 (3%)
Frame = +2
Query: 398 LKLIDQQNHNKIAFGDSKDKTSK--KVSWKFTPVLENNRVYFKIMSTEDKQYL 550
+ ++ NHN A DS++ K K K P+ E N+ + + DK+ L
Sbjct: 762 ISFVESNNHNGEAGSDSREPVKKFFKDHLKVKPI-EENKAFMTFVIPHDKENL 813
>At3g15380.1 68416.m01950 choline transporter-related contains weak
similarity to CD92 protein [Homo sapiens]
gi|16945323|emb|CAC82175
Length = 700
Score = 27.1 bits (57), Expect = 9.9
Identities = 13/40 (32%), Positives = 18/40 (45%), Gaps = 2/40 (5%)
Frame = +3
Query: 468 KSPGSLPP--CWKTTEYTSRSCPPKTNST*SSITRKVLVM 581
K G++P W+ YTSR C T S+ R +M
Sbjct: 522 KVSGTIPDHWFWRMAHYTSRGCLKSVEWTIKSVNRNAYIM 561
>At2g36390.1 68415.m04466 1,4-alpha-glucan branching enzyme / starch
branching enzyme class II (SBE2-1) nearly identical to
starch branching enzyme class II [Arabidopsis thaliana]
GI:619939
Length = 858
Score = 27.1 bits (57), Expect = 9.9
Identities = 12/48 (25%), Positives = 22/48 (45%)
Frame = -3
Query: 193 FRAFGDSGLVFTNDDTHIQLLCQYVISSWCKCGVRSQRTHGEDEGKQS 50
F DS FT+D H C +++ + C+ V +D+ ++S
Sbjct: 799 FNRLDDSAEFFTSDGRHDDRPCSFMVYAPCRTAVVYAAVDDDDDDERS 846
>At2g17033.2 68415.m01965 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 505
Score = 27.1 bits (57), Expect = 9.9
Identities = 34/123 (27%), Positives = 53/123 (43%), Gaps = 9/123 (7%)
Frame = -1
Query: 366 TVCSVKITLNWMGK*DLTISFPSFVHNW*AKSMVFLLPFSIRRFTASLITSPFFSFRYSE 187
TVCS + L+ G D + P + +W K F +PFSIR + + L + P +
Sbjct: 314 TVCS-NMVLSSYGAHD---ALPQ-MGSWLQKLKGFNVPFSIRTYNSVLNSCPTIISMLKD 368
Query: 186 HLAIAVSYSPMTTLIYSCSA------STSSVLGASV---ALEASAHTARTKANKVSLILA 34
+ VS S + T + A + SSVL ++ A+E + LIL
Sbjct: 369 LDSCPVSLSELRTFLNEDEALLVHELTQSSVLDEAIEWNAVEGKLDLHGMHLSSSYLILL 428
Query: 33 QWL 25
QW+
Sbjct: 429 QWM 431
>At2g17033.1 68415.m01964 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 504
Score = 27.1 bits (57), Expect = 9.9
Identities = 34/123 (27%), Positives = 53/123 (43%), Gaps = 9/123 (7%)
Frame = -1
Query: 366 TVCSVKITLNWMGK*DLTISFPSFVHNW*AKSMVFLLPFSIRRFTASLITSPFFSFRYSE 187
TVCS + L+ G D + P + +W K F +PFSIR + + L + P +
Sbjct: 313 TVCS-NMVLSSYGAHD---ALPQ-MGSWLQKLKGFNVPFSIRTYNSVLNSCPTIISMLKD 367
Query: 186 HLAIAVSYSPMTTLIYSCSA------STSSVLGASV---ALEASAHTARTKANKVSLILA 34
+ VS S + T + A + SSVL ++ A+E + LIL
Sbjct: 368 LDSCPVSLSELRTFLNEDEALLVHELTQSSVLDEAIEWNAVEGKLDLHGMHLSSSYLILL 427
Query: 33 QWL 25
QW+
Sbjct: 428 QWM 430
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,777,110
Number of Sequences: 28952
Number of extensions: 301287
Number of successful extensions: 1112
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 1072
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1111
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1236350304
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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