BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fner10g02f (583 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyce... 35 0.008 SPCC364.04c |||CASP family protein|Schizosaccharomyces pombe|chr... 30 0.21 SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 30 0.21 SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 30 0.28 SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 30 0.28 SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7 |S... 29 0.50 SPBC646.09c |int6|yin6|translation initiation factor eIF3e|Schiz... 29 0.65 SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces pomb... 28 0.87 SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 28 1.1 SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78 |Schizosacch... 28 1.1 SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pomb... 27 1.5 SPCC417.07c |mto1|mbo1, mod20|MT organizer Mto1|Schizosaccharomy... 27 2.0 SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 27 2.6 SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces po... 27 2.6 SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase Swr1|Schizo... 27 2.6 SPBC1709.09 |||mitochondrial translation termination factor|Schi... 26 3.5 SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation... 26 3.5 SPBC609.05 |pob3||FACT complex component Pob3|Schizosaccharomyce... 26 4.6 SPAC23H4.11c |cnl2||centromere localized protein Cnl2|Schizosacc... 26 4.6 SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces p... 26 4.6 SPBC649.05 |cut12|stf1|spindle pole body protein Cut12 |Schizosa... 25 6.1 SPCC188.08c |ubp22|ubp5|ubiquitin C-terminal hydrolase Ubp22|Sch... 25 6.1 SPBC1718.03 |ker1||DNA-directed RNA polymerase I complex subunit... 25 8.1 SPCC18B5.01c |bfr1|hba2, SPCPJ732.04c|brefeldin A efflux transpo... 25 8.1 SPAC10F6.10 |||protein kinase, RIO family |Schizosaccharomyces p... 25 8.1 SPBC27B12.12c |||CorA family magnesium ion transporter |Schizosa... 25 8.1 SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces pomb... 25 8.1 >SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyces pombe|chr 3|||Manual Length = 238 Score = 35.1 bits (77), Expect = 0.008 Identities = 28/107 (26%), Positives = 55/107 (51%), Gaps = 6/107 (5%) Frame = +1 Query: 187 TLEQQFNSLTKSKDAQD---FSKAWKDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQ 357 T+E ++SL KSK + F + ++ + +L N + + L + +A KA+++LEQ Sbjct: 8 TIELDYDSL-KSKISNFNSIFDRFLQEERKKLLNNKNEYLRQLS-EINEAQKKAEKSLEQ 65 Query: 358 SRQNIERTAEELRKAHPD---VEKNATALREKLQAAVQNTVQESQKL 489 + + E L K H + E+ + +EKL A ++ + S++L Sbjct: 66 TEARKQNFTELLEKEHEEQAITEQEIFSFQEKLDAMLKRKQKLSEEL 112 >SPCC364.04c |||CASP family protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 633 Score = 30.3 bits (65), Expect = 0.21 Identities = 25/80 (31%), Positives = 38/80 (47%), Gaps = 8/80 (10%) Frame = +1 Query: 334 KAKEALE---QSRQNIERTAEELRKAHPDVEKNATALREKLQAAV-----QNTVQESQKL 489 K KE +E Q ++ +ER E LRK D K+ + +AA+ + + E QKL Sbjct: 128 KEKEEMEGSLQGKEKLEREVENLRK-ELDKYKDLVETEAEKRAAITKEECEKSWLEQQKL 186 Query: 490 AKKVSSNVQETNEKLAPKIK 549 K + T +KL KI+ Sbjct: 187 YKDMEQENASTIQKLTSKIR 206 >SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 419 Score = 30.3 bits (65), Expect = 0.21 Identities = 17/81 (20%), Positives = 38/81 (46%) Frame = +1 Query: 286 NAFAKSLQGALGDANGKAKEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQN 465 N + ++ AL + KA + LE+ ++ E + EE+ H + T+ + + + Sbjct: 337 NLVSLAIYEALYEKFLKACKDLEEVSKSYEESREEIEALHETFTEEVTSFQSTKRLKEEK 396 Query: 466 TVQESQKLAKKVSSNVQETNE 528 +QE ++ K + Q+ +E Sbjct: 397 IIQEKSRVDKMIDEYRQKLSE 417 >SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 412 Score = 29.9 bits (64), Expect = 0.28 Identities = 26/109 (23%), Positives = 47/109 (43%) Frame = +1 Query: 106 LAQGAMVRRDAPDFFKDIEHHTKEFHKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQL 285 L + V R F + +EH+ K+LE+Q + L +SKDA A S+ Sbjct: 193 LKKSKSVPRLRGQFMEPVEHN-HPLSKSLEEQSSFLEQSKDASSNLTACNRSGSSLSSNF 251 Query: 286 NAFAKSLQGALGDANGKAKEALEQSRQNIERTAEELRKAHPDVEKNATA 432 + S + +L N K++ +L+ ++ R P+V + +A Sbjct: 252 YSSRLSKKTSLASLN-KSRASLQHKIMSLSRNIIRRVFHKPEVHLDPSA 299 >SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 168 Score = 29.9 bits (64), Expect = 0.28 Identities = 13/46 (28%), Positives = 25/46 (54%) Frame = +1 Query: 253 KDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEE 390 KDG+++ +LN FAK L + + +A + + IE+++ E Sbjct: 111 KDGTDAFANELNLFAKKLGFSKNSFDARALDTESEDETEIEKSSSE 156 >SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7 |Schizosaccharomyces pombe|chr 2|||Manual Length = 272 Score = 29.1 bits (62), Expect = 0.50 Identities = 17/69 (24%), Positives = 38/69 (55%) Frame = +1 Query: 334 KAKEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSSNV 513 K+++ LE S Q +E E + P+V++ +EK ++ V+ +E +K+S N+ Sbjct: 132 KSEKPLETS-QKVEIETVETKPGEPEVKQETNLQKEKKESKVKLESKE-----EKISRNL 185 Query: 514 QETNEKLAP 540 + ++ ++P Sbjct: 186 RSSSRSISP 194 >SPBC646.09c |int6|yin6|translation initiation factor eIF3e|Schizosaccharomyces pombe|chr 2|||Manual Length = 501 Score = 28.7 bits (61), Expect = 0.65 Identities = 18/54 (33%), Positives = 29/54 (53%), Gaps = 2/54 (3%) Frame = +1 Query: 232 QDFSKAWKDGSESVLQQLNAFAKSLQGALGD-ANGKAKEALEQSR-QNIERTAE 387 +D + A+ + SVLQ+L+ + +QG LG N AL Q + QN++ E Sbjct: 79 EDMANAFAEKRRSVLQELSELEEEVQGILGVLENPDLIAALRQDKGQNLQHLQE 132 >SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces pombe|chr 2|||Manual Length = 175 Score = 28.3 bits (60), Expect = 0.87 Identities = 24/88 (27%), Positives = 45/88 (51%) Frame = +1 Query: 157 IEHHTKEFHKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQGALGDANGK 336 IE K F K ++ NS K+ +A F +G++ ++ + A ++L L + Sbjct: 73 IESSMKSF-KPVKIDLNSQLKAINA--FEAKASEGAKKNVELVKAELQNLSATLKN---- 125 Query: 337 AKEALEQSRQNIERTAEELRKAHPDVEK 420 +EQ+R E T E++++A P++EK Sbjct: 126 ----IEQARPTEEITIEDMKQAVPEIEK 149 >SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizosaccharomyces pombe|chr 1|||Manual Length = 899 Score = 27.9 bits (59), Expect = 1.1 Identities = 18/70 (25%), Positives = 32/70 (45%), Gaps = 3/70 (4%) Frame = +1 Query: 127 RRDAPDFFKDIEHHTKEFHKTLEQQFNSLT---KSKDAQDFSKAWKDGSESVLQQLNAFA 297 ++ DFFK + ++ H TL ++ NSL+ +K + G + +LN Sbjct: 56 KKSEQDFFKMLSSRDRDAHSTLRKRSNSLSSFLSTKSTSASENKFHGGLNWLSLKLNLLL 115 Query: 298 KSLQGALGDA 327 + LQG + A Sbjct: 116 R-LQGRMNSA 124 >SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78 |Schizosaccharomyces pombe|chr 2|||Manual Length = 803 Score = 27.9 bits (59), Expect = 1.1 Identities = 24/74 (32%), Positives = 33/74 (44%), Gaps = 6/74 (8%) Frame = +1 Query: 187 TLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQGA------LGDANGKAKEA 348 T++ + SL K D + ++ ES L L L A L D+ KAK Sbjct: 354 TIQIELESLRKETDTTSVER--REKLESKLTDLKEEQDKLSAAWEEERKLLDSIKKAKTE 411 Query: 349 LEQSRQNIERTAEE 390 LEQ+R +ERT E Sbjct: 412 LEQARIELERTQRE 425 >SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 631 Score = 27.5 bits (58), Expect = 1.5 Identities = 10/20 (50%), Positives = 13/20 (65%) Frame = -1 Query: 364 ASTVPKPPWPCRSRLRALPG 305 AST+ K PWP + L +PG Sbjct: 38 ASTLEKEPWPASTALLVMPG 57 >SPCC417.07c |mto1|mbo1, mod20|MT organizer Mto1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1115 Score = 27.1 bits (57), Expect = 2.0 Identities = 17/73 (23%), Positives = 38/73 (52%) Frame = +1 Query: 349 LEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSSNVQETNE 528 ++ Q+IE T L K D+E++ +++ + V + Q+ ++++ +Q+T E Sbjct: 496 MKTQEQSIELT--RLYKQLQDIEEDYENKLMRMEQQWREDVDQLQEYVEEITQELQDTKE 553 Query: 529 KLAPKIKAAYDDF 567 L+ K + DD+ Sbjct: 554 VLSKSSKES-DDY 565 >SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces pombe|chr 2|||Manual Length = 1485 Score = 26.6 bits (56), Expect = 2.6 Identities = 12/42 (28%), Positives = 19/42 (45%), Gaps = 2/42 (4%) Frame = +1 Query: 133 DAPDFFKDIEHHTKEFHKTLEQ--QFNSLTKSKDAQDFSKAW 252 D +F D++ H K FH E+ + + +K D K W Sbjct: 665 DMKSYFSDLDRHMKYFHAMQEKDAELIEMAFAKKKADVRKEW 706 >SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces pombe|chr 1|||Manual Length = 670 Score = 26.6 bits (56), Expect = 2.6 Identities = 17/57 (29%), Positives = 26/57 (45%), Gaps = 5/57 (8%) Frame = +1 Query: 301 SLQGALGDANGKAKEALEQSRQNIE-----RTAEELRKAHPDVEKNATALREKLQAA 456 S++ L + N + KE +E + RT +E EKN LRE+L+ A Sbjct: 520 SMKDDLTEMNQRLKEQIESYENEVNSEITSRTLKEFETLKTQYEKNLCNLREQLKTA 576 >SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase Swr1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1288 Score = 26.6 bits (56), Expect = 2.6 Identities = 22/74 (29%), Positives = 32/74 (43%) Frame = +1 Query: 262 SESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEELRKAHPDVEKNATALRE 441 S + + A K L GA KAKE ++ R +RTA E+RK +E+ R Sbjct: 143 SRRISGMILAHFKRLSGA---DEKKAKEEDKRIRLLAKRTAWEIRKKWKVIEREVRRRRA 199 Query: 442 KLQAAVQNTVQESQ 483 + A Q + Q Sbjct: 200 ERAAEAQRVAGKEQ 213 >SPBC1709.09 |||mitochondrial translation termination factor|Schizosaccharomyces pombe|chr 2|||Manual Length = 244 Score = 26.2 bits (55), Expect = 3.5 Identities = 18/71 (25%), Positives = 35/71 (49%) Frame = +1 Query: 364 QNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSSNVQETNEKLAPK 543 +NI + E+ R + NA+ + K Q + T++ Q+LAK + +++ E+L+ Sbjct: 136 KNILKAIEDSRYPFVANKLNASTIEVKPQ---RTTLESRQQLAKVLEGYAKDSREQLSAM 192 Query: 544 IKAAYDDFAKN 576 + AKN Sbjct: 193 RTELKKEIAKN 203 >SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation factor eIF2 beta subunit|Schizosaccharomyces pombe|chr 1|||Manual Length = 321 Score = 26.2 bits (55), Expect = 3.5 Identities = 16/54 (29%), Positives = 25/54 (46%), Gaps = 1/54 (1%) Frame = +1 Query: 169 TKEFHKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQ-GALGDA 327 +K+ + E+Q +T DFS K + L+AF K L+ + GDA Sbjct: 89 SKKSSASAEEQTEDITTESGELDFSSMKKKKKKKKSADLSAFEKELEASSTGDA 142 >SPBC609.05 |pob3||FACT complex component Pob3|Schizosaccharomyces pombe|chr 2|||Manual Length = 512 Score = 25.8 bits (54), Expect = 4.6 Identities = 12/24 (50%), Positives = 17/24 (70%), Gaps = 2/24 (8%) Frame = +1 Query: 496 KVSSNVQET--NEKLAPKIKAAYD 561 +V N++ET EK A K+KA+YD Sbjct: 299 EVDLNIEETVLKEKYADKVKASYD 322 >SPAC23H4.11c |cnl2||centromere localized protein Cnl2|Schizosaccharomyces pombe|chr 1|||Manual Length = 188 Score = 25.8 bits (54), Expect = 4.6 Identities = 17/75 (22%), Positives = 36/75 (48%), Gaps = 2/75 (2%) Frame = +1 Query: 340 KEALEQSRQNIERTAEELRKAHPD--VEKNATALREKLQAAVQNTVQESQKLAKKVSSNV 513 ++ L + R NI ++ + K+ D + N L+ A+ + V+E ++ Sbjct: 53 QKRLAKLRANIHLESQVIGKSRIDRMLATNVEKLQTVSHASTLHDVEEFYTSHSAKPLDI 112 Query: 514 QETNEKLAPKIKAAY 558 E NE+L+ +++AY Sbjct: 113 SEINERLSEAVQSAY 127 >SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces pombe|chr 1|||Manual Length = 394 Score = 25.8 bits (54), Expect = 4.6 Identities = 16/64 (25%), Positives = 34/64 (53%), Gaps = 1/64 (1%) Frame = +1 Query: 97 CIALAQGAMVRRDAPDFFKDIEHHTKEFH-KTLEQQFNSLTKSKDAQDFSKAWKDGSESV 273 C + +G +RR P +++ I ++ KTL + FN+ + +++ + KA ++G V Sbjct: 13 CEYVFKGDGLRRVPPYYYEYITFAKLRWYGKTLLEVFNTEFRDRESGYYEKAIRNGQVKV 72 Query: 274 LQQL 285 Q+ Sbjct: 73 NNQI 76 >SPBC649.05 |cut12|stf1|spindle pole body protein Cut12 |Schizosaccharomyces pombe|chr 2|||Manual Length = 548 Score = 25.4 bits (53), Expect = 6.1 Identities = 10/29 (34%), Positives = 18/29 (62%) Frame = +1 Query: 391 LRKAHPDVEKNATALREKLQAAVQNTVQE 477 L AH D+E T++RE+L++ + +E Sbjct: 267 LDSAHSDLELELTSIRERLESLILEKQEE 295 >SPCC188.08c |ubp22|ubp5|ubiquitin C-terminal hydrolase Ubp22|Schizosaccharomyces pombe|chr 3|||Manual Length = 1108 Score = 25.4 bits (53), Expect = 6.1 Identities = 13/32 (40%), Positives = 19/32 (59%) Frame = +1 Query: 328 NGKAKEALEQSRQNIERTAEELRKAHPDVEKN 423 N K KE E++ + EELR+A PD E++ Sbjct: 22 NEKLKEDFEENVSIDVKIHEELRRALPDYEES 53 >SPBC1718.03 |ker1||DNA-directed RNA polymerase I complex subunit subunit Ker1|Schizosaccharomyces pombe|chr 2|||Manual Length = 147 Score = 25.0 bits (52), Expect = 8.1 Identities = 12/20 (60%), Positives = 13/20 (65%) Frame = +1 Query: 253 KDGSESVLQQLNAFAKSLQG 312 K GSESVL QLN L+G Sbjct: 40 KSGSESVLSQLNRVLMYLKG 59 >SPCC18B5.01c |bfr1|hba2, SPCPJ732.04c|brefeldin A efflux transporter Bfr1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1530 Score = 25.0 bits (52), Expect = 8.1 Identities = 13/26 (50%), Positives = 16/26 (61%) Frame = -3 Query: 443 FSRRAVAFFSTSGWALRSSSAVRSMF 366 FSR V FFS AL+S S + +MF Sbjct: 570 FSRGGVLFFSILFCALQSLSEIANMF 595 >SPAC10F6.10 |||protein kinase, RIO family |Schizosaccharomyces pombe|chr 1|||Manual Length = 521 Score = 25.0 bits (52), Expect = 8.1 Identities = 10/24 (41%), Positives = 15/24 (62%) Frame = +1 Query: 319 GDANGKAKEALEQSRQNIERTAEE 390 G NG+AKE E+ R ++T E+ Sbjct: 473 GKGNGRAKETPEEKRARKKKTKED 496 >SPBC27B12.12c |||CorA family magnesium ion transporter |Schizosaccharomyces pombe|chr 2|||Manual Length = 803 Score = 25.0 bits (52), Expect = 8.1 Identities = 10/24 (41%), Positives = 14/24 (58%) Frame = -1 Query: 370 CSASTVPKPPWPCRSRLRALPGDS 299 C +T+PKPP+ + L LP S Sbjct: 290 CPHTTIPKPPYQSDTDLTELPTKS 313 >SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces pombe|chr 1|||Manual Length = 432 Score = 25.0 bits (52), Expect = 8.1 Identities = 13/51 (25%), Positives = 26/51 (50%) Frame = +1 Query: 355 QSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSS 507 ++RQ E A+ +RK+ PDV + ++ +V + +E + +SS Sbjct: 284 ENRQFSEYVADRIRKSFPDVSFKNLHVLSCMEDSVMSPFRELGSVTSSMSS 334 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.313 0.124 0.340 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,794,723 Number of Sequences: 5004 Number of extensions: 30423 Number of successful extensions: 144 Number of sequences better than 10.0: 27 Number of HSP's better than 10.0 without gapping: 142 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 143 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 250133048 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.2 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 42 (22.0 bits)
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