SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fner10g02f
         (583 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyce...    35   0.008
SPCC364.04c |||CASP family protein|Schizosaccharomyces pombe|chr...    30   0.21 
SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr 1|...    30   0.21 
SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|...    30   0.28 
SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    30   0.28 
SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7 |S...    29   0.50 
SPBC646.09c |int6|yin6|translation initiation factor eIF3e|Schiz...    29   0.65 
SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces pomb...    28   0.87 
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos...    28   1.1  
SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78 |Schizosacch...    28   1.1  
SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pomb...    27   1.5  
SPCC417.07c |mto1|mbo1, mod20|MT organizer Mto1|Schizosaccharomy...    27   2.0  
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces...    27   2.6  
SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces po...    27   2.6  
SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase Swr1|Schizo...    27   2.6  
SPBC1709.09 |||mitochondrial translation termination factor|Schi...    26   3.5  
SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation...    26   3.5  
SPBC609.05 |pob3||FACT complex component Pob3|Schizosaccharomyce...    26   4.6  
SPAC23H4.11c |cnl2||centromere localized protein Cnl2|Schizosacc...    26   4.6  
SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces p...    26   4.6  
SPBC649.05 |cut12|stf1|spindle pole body protein Cut12 |Schizosa...    25   6.1  
SPCC188.08c |ubp22|ubp5|ubiquitin C-terminal hydrolase Ubp22|Sch...    25   6.1  
SPBC1718.03 |ker1||DNA-directed RNA polymerase I complex subunit...    25   8.1  
SPCC18B5.01c |bfr1|hba2, SPCPJ732.04c|brefeldin A efflux transpo...    25   8.1  
SPAC10F6.10 |||protein kinase, RIO family |Schizosaccharomyces p...    25   8.1  
SPBC27B12.12c |||CorA family magnesium ion transporter |Schizosa...    25   8.1  
SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces pomb...    25   8.1  

>SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 238

 Score = 35.1 bits (77), Expect = 0.008
 Identities = 28/107 (26%), Positives = 55/107 (51%), Gaps = 6/107 (5%)
 Frame = +1

Query: 187 TLEQQFNSLTKSKDAQD---FSKAWKDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQ 357
           T+E  ++SL KSK +     F +  ++  + +L   N + + L   + +A  KA+++LEQ
Sbjct: 8   TIELDYDSL-KSKISNFNSIFDRFLQEERKKLLNNKNEYLRQLS-EINEAQKKAEKSLEQ 65

Query: 358 SRQNIERTAEELRKAHPD---VEKNATALREKLQAAVQNTVQESQKL 489
           +    +   E L K H +    E+   + +EKL A ++   + S++L
Sbjct: 66  TEARKQNFTELLEKEHEEQAITEQEIFSFQEKLDAMLKRKQKLSEEL 112


>SPCC364.04c |||CASP family protein|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 633

 Score = 30.3 bits (65), Expect = 0.21
 Identities = 25/80 (31%), Positives = 38/80 (47%), Gaps = 8/80 (10%)
 Frame = +1

Query: 334 KAKEALE---QSRQNIERTAEELRKAHPDVEKNATALREKLQAAV-----QNTVQESQKL 489
           K KE +E   Q ++ +ER  E LRK   D  K+      + +AA+     + +  E QKL
Sbjct: 128 KEKEEMEGSLQGKEKLEREVENLRK-ELDKYKDLVETEAEKRAAITKEECEKSWLEQQKL 186

Query: 490 AKKVSSNVQETNEKLAPKIK 549
            K +      T +KL  KI+
Sbjct: 187 YKDMEQENASTIQKLTSKIR 206


>SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 419

 Score = 30.3 bits (65), Expect = 0.21
 Identities = 17/81 (20%), Positives = 38/81 (46%)
 Frame = +1

Query: 286 NAFAKSLQGALGDANGKAKEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQN 465
           N  + ++  AL +   KA + LE+  ++ E + EE+   H    +  T+ +   +   + 
Sbjct: 337 NLVSLAIYEALYEKFLKACKDLEEVSKSYEESREEIEALHETFTEEVTSFQSTKRLKEEK 396

Query: 466 TVQESQKLAKKVSSNVQETNE 528
            +QE  ++ K +    Q+ +E
Sbjct: 397 IIQEKSRVDKMIDEYRQKLSE 417


>SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 412

 Score = 29.9 bits (64), Expect = 0.28
 Identities = 26/109 (23%), Positives = 47/109 (43%)
 Frame = +1

Query: 106 LAQGAMVRRDAPDFFKDIEHHTKEFHKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQL 285
           L +   V R    F + +EH+     K+LE+Q + L +SKDA     A      S+    
Sbjct: 193 LKKSKSVPRLRGQFMEPVEHN-HPLSKSLEEQSSFLEQSKDASSNLTACNRSGSSLSSNF 251

Query: 286 NAFAKSLQGALGDANGKAKEALEQSRQNIERTAEELRKAHPDVEKNATA 432
            +   S + +L   N K++ +L+    ++ R         P+V  + +A
Sbjct: 252 YSSRLSKKTSLASLN-KSRASLQHKIMSLSRNIIRRVFHKPEVHLDPSA 299


>SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 168

 Score = 29.9 bits (64), Expect = 0.28
 Identities = 13/46 (28%), Positives = 25/46 (54%)
 Frame = +1

Query: 253 KDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEE 390
           KDG+++   +LN FAK L  +    + +A +   +    IE+++ E
Sbjct: 111 KDGTDAFANELNLFAKKLGFSKNSFDARALDTESEDETEIEKSSSE 156


>SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 272

 Score = 29.1 bits (62), Expect = 0.50
 Identities = 17/69 (24%), Positives = 38/69 (55%)
 Frame = +1

Query: 334 KAKEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSSNV 513
           K+++ LE S Q +E    E +   P+V++     +EK ++ V+   +E     +K+S N+
Sbjct: 132 KSEKPLETS-QKVEIETVETKPGEPEVKQETNLQKEKKESKVKLESKE-----EKISRNL 185

Query: 514 QETNEKLAP 540
           + ++  ++P
Sbjct: 186 RSSSRSISP 194


>SPBC646.09c |int6|yin6|translation initiation factor
           eIF3e|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 501

 Score = 28.7 bits (61), Expect = 0.65
 Identities = 18/54 (33%), Positives = 29/54 (53%), Gaps = 2/54 (3%)
 Frame = +1

Query: 232 QDFSKAWKDGSESVLQQLNAFAKSLQGALGD-ANGKAKEALEQSR-QNIERTAE 387
           +D + A+ +   SVLQ+L+   + +QG LG   N     AL Q + QN++   E
Sbjct: 79  EDMANAFAEKRRSVLQELSELEEEVQGILGVLENPDLIAALRQDKGQNLQHLQE 132


>SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 175

 Score = 28.3 bits (60), Expect = 0.87
 Identities = 24/88 (27%), Positives = 45/88 (51%)
 Frame = +1

Query: 157 IEHHTKEFHKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQGALGDANGK 336
           IE   K F K ++   NS  K+ +A  F     +G++  ++ + A  ++L   L +    
Sbjct: 73  IESSMKSF-KPVKIDLNSQLKAINA--FEAKASEGAKKNVELVKAELQNLSATLKN---- 125

Query: 337 AKEALEQSRQNIERTAEELRKAHPDVEK 420
               +EQ+R   E T E++++A P++EK
Sbjct: 126 ----IEQARPTEEITIEDMKQAVPEIEK 149


>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
           Plc1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 899

 Score = 27.9 bits (59), Expect = 1.1
 Identities = 18/70 (25%), Positives = 32/70 (45%), Gaps = 3/70 (4%)
 Frame = +1

Query: 127 RRDAPDFFKDIEHHTKEFHKTLEQQFNSLT---KSKDAQDFSKAWKDGSESVLQQLNAFA 297
           ++   DFFK +    ++ H TL ++ NSL+    +K        +  G   +  +LN   
Sbjct: 56  KKSEQDFFKMLSSRDRDAHSTLRKRSNSLSSFLSTKSTSASENKFHGGLNWLSLKLNLLL 115

Query: 298 KSLQGALGDA 327
           + LQG +  A
Sbjct: 116 R-LQGRMNSA 124


>SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 803

 Score = 27.9 bits (59), Expect = 1.1
 Identities = 24/74 (32%), Positives = 33/74 (44%), Gaps = 6/74 (8%)
 Frame = +1

Query: 187 TLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQGA------LGDANGKAKEA 348
           T++ +  SL K  D     +  ++  ES L  L      L  A      L D+  KAK  
Sbjct: 354 TIQIELESLRKETDTTSVER--REKLESKLTDLKEEQDKLSAAWEEERKLLDSIKKAKTE 411

Query: 349 LEQSRQNIERTAEE 390
           LEQ+R  +ERT  E
Sbjct: 412 LEQARIELERTQRE 425


>SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 631

 Score = 27.5 bits (58), Expect = 1.5
 Identities = 10/20 (50%), Positives = 13/20 (65%)
 Frame = -1

Query: 364 ASTVPKPPWPCRSRLRALPG 305
           AST+ K PWP  + L  +PG
Sbjct: 38  ASTLEKEPWPASTALLVMPG 57


>SPCC417.07c |mto1|mbo1, mod20|MT organizer Mto1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1115

 Score = 27.1 bits (57), Expect = 2.0
 Identities = 17/73 (23%), Positives = 38/73 (52%)
 Frame = +1

Query: 349 LEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSSNVQETNE 528
           ++   Q+IE T   L K   D+E++      +++   +  V + Q+  ++++  +Q+T E
Sbjct: 496 MKTQEQSIELT--RLYKQLQDIEEDYENKLMRMEQQWREDVDQLQEYVEEITQELQDTKE 553

Query: 529 KLAPKIKAAYDDF 567
            L+   K + DD+
Sbjct: 554 VLSKSSKES-DDY 565


>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1485

 Score = 26.6 bits (56), Expect = 2.6
 Identities = 12/42 (28%), Positives = 19/42 (45%), Gaps = 2/42 (4%)
 Frame = +1

Query: 133 DAPDFFKDIEHHTKEFHKTLEQ--QFNSLTKSKDAQDFSKAW 252
           D   +F D++ H K FH   E+  +   +  +K   D  K W
Sbjct: 665 DMKSYFSDLDRHMKYFHAMQEKDAELIEMAFAKKKADVRKEW 706


>SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 670

 Score = 26.6 bits (56), Expect = 2.6
 Identities = 17/57 (29%), Positives = 26/57 (45%), Gaps = 5/57 (8%)
 Frame = +1

Query: 301 SLQGALGDANGKAKEALEQSRQNIE-----RTAEELRKAHPDVEKNATALREKLQAA 456
           S++  L + N + KE +E     +      RT +E        EKN   LRE+L+ A
Sbjct: 520 SMKDDLTEMNQRLKEQIESYENEVNSEITSRTLKEFETLKTQYEKNLCNLREQLKTA 576


>SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase
           Swr1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1288

 Score = 26.6 bits (56), Expect = 2.6
 Identities = 22/74 (29%), Positives = 32/74 (43%)
 Frame = +1

Query: 262 SESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEELRKAHPDVEKNATALRE 441
           S  +   + A  K L GA      KAKE  ++ R   +RTA E+RK    +E+     R 
Sbjct: 143 SRRISGMILAHFKRLSGA---DEKKAKEEDKRIRLLAKRTAWEIRKKWKVIEREVRRRRA 199

Query: 442 KLQAAVQNTVQESQ 483
           +  A  Q    + Q
Sbjct: 200 ERAAEAQRVAGKEQ 213


>SPBC1709.09 |||mitochondrial translation termination
           factor|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 244

 Score = 26.2 bits (55), Expect = 3.5
 Identities = 18/71 (25%), Positives = 35/71 (49%)
 Frame = +1

Query: 364 QNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSSNVQETNEKLAPK 543
           +NI +  E+ R      + NA+ +  K Q   + T++  Q+LAK +    +++ E+L+  
Sbjct: 136 KNILKAIEDSRYPFVANKLNASTIEVKPQ---RTTLESRQQLAKVLEGYAKDSREQLSAM 192

Query: 544 IKAAYDDFAKN 576
                 + AKN
Sbjct: 193 RTELKKEIAKN 203


>SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation
           factor eIF2 beta subunit|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 321

 Score = 26.2 bits (55), Expect = 3.5
 Identities = 16/54 (29%), Positives = 25/54 (46%), Gaps = 1/54 (1%)
 Frame = +1

Query: 169 TKEFHKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQ-GALGDA 327
           +K+   + E+Q   +T      DFS   K   +     L+AF K L+  + GDA
Sbjct: 89  SKKSSASAEEQTEDITTESGELDFSSMKKKKKKKKSADLSAFEKELEASSTGDA 142


>SPBC609.05 |pob3||FACT complex component Pob3|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 512

 Score = 25.8 bits (54), Expect = 4.6
 Identities = 12/24 (50%), Positives = 17/24 (70%), Gaps = 2/24 (8%)
 Frame = +1

Query: 496 KVSSNVQET--NEKLAPKIKAAYD 561
           +V  N++ET   EK A K+KA+YD
Sbjct: 299 EVDLNIEETVLKEKYADKVKASYD 322


>SPAC23H4.11c |cnl2||centromere localized protein
           Cnl2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 188

 Score = 25.8 bits (54), Expect = 4.6
 Identities = 17/75 (22%), Positives = 36/75 (48%), Gaps = 2/75 (2%)
 Frame = +1

Query: 340 KEALEQSRQNIERTAEELRKAHPD--VEKNATALREKLQAAVQNTVQESQKLAKKVSSNV 513
           ++ L + R NI   ++ + K+  D  +  N   L+    A+  + V+E          ++
Sbjct: 53  QKRLAKLRANIHLESQVIGKSRIDRMLATNVEKLQTVSHASTLHDVEEFYTSHSAKPLDI 112

Query: 514 QETNEKLAPKIKAAY 558
            E NE+L+  +++AY
Sbjct: 113 SEINERLSEAVQSAY 127


>SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 394

 Score = 25.8 bits (54), Expect = 4.6
 Identities = 16/64 (25%), Positives = 34/64 (53%), Gaps = 1/64 (1%)
 Frame = +1

Query: 97  CIALAQGAMVRRDAPDFFKDIEHHTKEFH-KTLEQQFNSLTKSKDAQDFSKAWKDGSESV 273
           C  + +G  +RR  P +++ I      ++ KTL + FN+  + +++  + KA ++G   V
Sbjct: 13  CEYVFKGDGLRRVPPYYYEYITFAKLRWYGKTLLEVFNTEFRDRESGYYEKAIRNGQVKV 72

Query: 274 LQQL 285
             Q+
Sbjct: 73  NNQI 76


>SPBC649.05 |cut12|stf1|spindle pole body protein Cut12
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 548

 Score = 25.4 bits (53), Expect = 6.1
 Identities = 10/29 (34%), Positives = 18/29 (62%)
 Frame = +1

Query: 391 LRKAHPDVEKNATALREKLQAAVQNTVQE 477
           L  AH D+E   T++RE+L++ +    +E
Sbjct: 267 LDSAHSDLELELTSIRERLESLILEKQEE 295


>SPCC188.08c |ubp22|ubp5|ubiquitin C-terminal hydrolase
           Ubp22|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1108

 Score = 25.4 bits (53), Expect = 6.1
 Identities = 13/32 (40%), Positives = 19/32 (59%)
 Frame = +1

Query: 328 NGKAKEALEQSRQNIERTAEELRKAHPDVEKN 423
           N K KE  E++     +  EELR+A PD E++
Sbjct: 22  NEKLKEDFEENVSIDVKIHEELRRALPDYEES 53


>SPBC1718.03 |ker1||DNA-directed RNA polymerase I complex subunit
           subunit Ker1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 147

 Score = 25.0 bits (52), Expect = 8.1
 Identities = 12/20 (60%), Positives = 13/20 (65%)
 Frame = +1

Query: 253 KDGSESVLQQLNAFAKSLQG 312
           K GSESVL QLN     L+G
Sbjct: 40  KSGSESVLSQLNRVLMYLKG 59


>SPCC18B5.01c |bfr1|hba2, SPCPJ732.04c|brefeldin A efflux
           transporter Bfr1|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1530

 Score = 25.0 bits (52), Expect = 8.1
 Identities = 13/26 (50%), Positives = 16/26 (61%)
 Frame = -3

Query: 443 FSRRAVAFFSTSGWALRSSSAVRSMF 366
           FSR  V FFS    AL+S S + +MF
Sbjct: 570 FSRGGVLFFSILFCALQSLSEIANMF 595


>SPAC10F6.10 |||protein kinase, RIO family |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 521

 Score = 25.0 bits (52), Expect = 8.1
 Identities = 10/24 (41%), Positives = 15/24 (62%)
 Frame = +1

Query: 319 GDANGKAKEALEQSRQNIERTAEE 390
           G  NG+AKE  E+ R   ++T E+
Sbjct: 473 GKGNGRAKETPEEKRARKKKTKED 496


>SPBC27B12.12c |||CorA family magnesium ion transporter
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 803

 Score = 25.0 bits (52), Expect = 8.1
 Identities = 10/24 (41%), Positives = 14/24 (58%)
 Frame = -1

Query: 370 CSASTVPKPPWPCRSRLRALPGDS 299
           C  +T+PKPP+   + L  LP  S
Sbjct: 290 CPHTTIPKPPYQSDTDLTELPTKS 313


>SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 432

 Score = 25.0 bits (52), Expect = 8.1
 Identities = 13/51 (25%), Positives = 26/51 (50%)
 Frame = +1

Query: 355 QSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSS 507
           ++RQ  E  A+ +RK+ PDV      +   ++ +V +  +E   +   +SS
Sbjct: 284 ENRQFSEYVADRIRKSFPDVSFKNLHVLSCMEDSVMSPFRELGSVTSSMSS 334


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.313    0.124    0.340 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,794,723
Number of Sequences: 5004
Number of extensions: 30423
Number of successful extensions: 144
Number of sequences better than 10.0: 27
Number of HSP's better than 10.0 without gapping: 142
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 143
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 250133048
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (22.0 bits)

- SilkBase 1999-2023 -