BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fner10f13r (727 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein (H... 38 0.009 At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 35 0.063 At2g27100.1 68415.m03256 C2H2 zinc-finger protein SERRATE (SE) i... 34 0.11 At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 33 0.15 At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 33 0.15 At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 33 0.19 At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock pro... 30 1.4 At3g49540.1 68416.m05414 expressed protein 30 1.4 At4g14830.1 68417.m02280 expressed protein 29 3.1 At2g28680.1 68415.m03486 cupin family protein similar to legumin... 29 4.1 At2g20360.1 68415.m02377 expressed protein 28 5.5 At1g66720.1 68414.m07584 S-adenosyl-L-methionine:carboxyl methyl... 28 5.5 At5g07100.2 68418.m00807 WRKY family transcription factor SPF1 p... 28 7.2 At5g07100.1 68418.m00806 WRKY family transcription factor SPF1 p... 28 7.2 At4g17520.1 68417.m02621 nuclear RNA-binding protein, putative s... 28 7.2 At1g76770.1 68414.m08934 heat shock protein-related contains sim... 28 7.2 At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containi... 28 7.2 At1g62570.1 68414.m07059 flavin-containing monooxygenase family ... 28 7.2 At1g28060.1 68414.m03435 small nuclear ribonucleoprotein family ... 28 7.2 At5g18190.1 68418.m02135 protein kinase family protein contains ... 27 9.6 At3g56100.1 68416.m06235 leucine-rich repeat transmembrane prote... 27 9.6 >At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein (HSP22.0-ER) identical to endomembrane-localized small heat shock protein GI:511795 from [Arabidopsis thaliana] Length = 195 Score = 37.5 bits (83), Expect = 0.009 Identities = 20/48 (41%), Positives = 31/48 (64%) Frame = -3 Query: 290 QFVRRYALPEGAAPETVESRLSSDGVLTITAPRKVPDAVKGERKVPIA 147 +F R++ LP+ E+V+++L + GVLTI + P+ VKG R V IA Sbjct: 132 KFWRQFKLPDNVDMESVKAKLEN-GVLTINLTKLSPEKVKGPRVVNIA 178 >At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein (HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853) (GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res. 17 (19), 7995 (1989)) Length = 157 Score = 34.7 bits (76), Expect = 0.063 Identities = 18/50 (36%), Positives = 31/50 (62%) Frame = -3 Query: 296 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPRKVPDAVKGERKVPIA 147 S +F RR+ LPE A E +++ + +GVL++T P KVP+ + + I+ Sbjct: 109 SGKFTRRFRLPENAKMEEIKASM-ENGVLSVTVP-KVPEKKPEVKSIDIS 156 >At2g27100.1 68415.m03256 C2H2 zinc-finger protein SERRATE (SE) identical to C2H2 zinc-finger protein SERRATE GI:14486602 from [Arabidopsis thaliana] Length = 720 Score = 33.9 bits (74), Expect = 0.11 Identities = 16/32 (50%), Positives = 17/32 (53%) Frame = -2 Query: 189 GTRRRQGRAKGAHRTDRSRSQGDQGPERGNPG 94 G R R GRA G R DRS Q + GNPG Sbjct: 582 GRRERDGRANGNDRNDRSEDQQRGDNDGGNPG 613 >At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HSP18.1-CI) identical to 18.2 kDa class I heat shock protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana]; contains Pfam profile: PF00011 Hsp20/alpha crystallin family Length = 161 Score = 33.5 bits (73), Expect = 0.15 Identities = 18/50 (36%), Positives = 30/50 (60%) Frame = -3 Query: 296 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPRKVPDAVKGERKVPIA 147 S +F+RR+ LPE A E V++ + +GVLT+ P K P+ + + I+ Sbjct: 111 SGKFMRRFRLPENAKMEEVKATM-ENGVLTVVVP-KAPEKKPQVKSIDIS 158 >At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HSP17.4-CI) identical to 17.4 kDa class I heat shock protein SP:P19036 from [Arabidopsis thaliana] Length = 156 Score = 33.5 bits (73), Expect = 0.15 Identities = 19/50 (38%), Positives = 32/50 (64%) Frame = -3 Query: 296 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPRKVPDAVKGERKVPIA 147 S +F+RR+ LPE A E V++ + +GVL++T P KV ++ + V I+ Sbjct: 108 SGKFMRRFRLPENAKVEEVKASM-ENGVLSVTVP-KVQESKPEVKSVDIS 155 >At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein (HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha crystallin family; identified in Scharf, K-D., et al, Cell Stress & Chaperones (2001) 6: 225-237. Length = 153 Score = 33.1 bits (72), Expect = 0.19 Identities = 15/35 (42%), Positives = 23/35 (65%) Frame = -3 Query: 296 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPR 192 S QF RR+ LPE + V++ + +GVLT+T P+ Sbjct: 105 SGQFTRRFRLPENVKMDQVKAAM-ENGVLTVTVPK 138 >At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock protein (HSP26.5-P) contains Pfam profile: PF00011 Hsp20/alpha crystallin family: identified in Scharf, K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237. Length = 134 Score = 30.3 bits (65), Expect = 1.4 Identities = 18/79 (22%), Positives = 33/79 (41%) Frame = -3 Query: 422 DKFQVNLDVQHFSPEEISVKTADGYIVVXXXXXXXXXXXGYISRQFVRRYALPEGAAPET 243 D ++D+ EEI V+ D ++ + F R++ LPE Sbjct: 35 DSHTFSVDLPGLRKEEIKVEIEDSIYLIIRTEATPMSPPDQPLKTFKRKFRLPESIDMIG 94 Query: 242 VESRLSSDGVLTITAPRKV 186 + + DGVLT+ P+++ Sbjct: 95 ISAGYE-DGVLTVIVPKRI 112 >At3g49540.1 68416.m05414 expressed protein Length = 166 Score = 30.3 bits (65), Expect = 1.4 Identities = 20/65 (30%), Positives = 35/65 (53%), Gaps = 4/65 (6%) Frame = -3 Query: 263 EGAAPETVESRLSSDGVLTITAPRKVPDAVKGERKV---PI-AQTGPVRKEIKDQSEGTQ 96 E AA E E+++ + + + AP KV AV+ E+K P+ A+ PV+ E + E ++ Sbjct: 100 ENAATENAEAKVEA---VAVAAPEKVEVAVEAEKKAEAEPVKAEAEPVKAEAEPVKEESK 156 Query: 95 DAENK 81 E + Sbjct: 157 QEEKE 161 >At4g14830.1 68417.m02280 expressed protein Length = 152 Score = 29.1 bits (62), Expect = 3.1 Identities = 13/31 (41%), Positives = 16/31 (51%) Frame = -3 Query: 284 VRRYALPEGAAPETVESRLSSDGVLTITAPR 192 V R+ LPE PE V DG L +T P+ Sbjct: 109 VWRFRLPESTRPELVTVDCDGDGELIVTVPK 139 >At2g28680.1 68415.m03486 cupin family protein similar to legumin (11S-globulin) from Ginkgo biloba [GI:949869], 11S globulin from Avena sativa [GI:472867]; contains a 11-S plant seed storage protein signature (PS00305) Length = 356 Score = 28.7 bits (61), Expect = 4.1 Identities = 21/64 (32%), Positives = 35/64 (54%), Gaps = 3/64 (4%) Frame = -3 Query: 296 SRQFV-RRYALPEGAAPETVESRLSSDGVLTITAPRKVPDAVKGERK--VPIAQTGPVRK 126 S +FV R + L E + V S+ + +G++ + A K+P+ KG+RK V P+ Sbjct: 141 STEFVGRAWDLDETTVKKLVGSQ-TGNGIVKVDASLKMPEPKKGDRKGFVLNCLEAPLDV 199 Query: 125 EIKD 114 +IKD Sbjct: 200 DIKD 203 >At2g20360.1 68415.m02377 expressed protein Length = 402 Score = 28.3 bits (60), Expect = 5.5 Identities = 13/26 (50%), Positives = 16/26 (61%) Frame = +3 Query: 132 NGTGLCDGHLSLSLDGVGYLPRRGDG 209 NGT C SL+ GVG+L R+G G Sbjct: 36 NGTDNCRYSSSLATKGVGHLARKGTG 61 >At1g66720.1 68414.m07584 S-adenosyl-L-methionine:carboxyl methyltransferase family protein similar to defense-related protein cjs1 [Brassica carinata][GI:14009292][Mol Plant Pathol (2001) 2(3):159-169] Length = 352 Score = 28.3 bits (60), Expect = 5.5 Identities = 11/26 (42%), Positives = 16/26 (61%) Frame = -1 Query: 286 SSDVTRCLKARRLRLWNRDCHQTGFS 209 +S + + + R LWNRD H TGF+ Sbjct: 151 TSKIPKGISDRNSPLWNRDMHCTGFN 176 >At5g07100.2 68418.m00807 WRKY family transcription factor SPF1 protein - Ipomoea batatas (sweet potato), PIR:S51529 Length = 216 Score = 27.9 bits (59), Expect = 7.2 Identities = 11/40 (27%), Positives = 20/40 (50%) Frame = -2 Query: 228 VIRRGSHHHRAEEGTRRRQGRAKGAHRTDRSRSQGDQGPE 109 ++ +GSH+H + T+R A AH+ + D G + Sbjct: 72 IVYKGSHNHPKPQSTKRSSSTAIAAHQNSSNGDGKDIGED 111 >At5g07100.1 68418.m00806 WRKY family transcription factor SPF1 protein - Ipomoea batatas (sweet potato), PIR:S51529 Length = 309 Score = 27.9 bits (59), Expect = 7.2 Identities = 11/40 (27%), Positives = 20/40 (50%) Frame = -2 Query: 228 VIRRGSHHHRAEEGTRRRQGRAKGAHRTDRSRSQGDQGPE 109 ++ +GSH+H + T+R A AH+ + D G + Sbjct: 165 IVYKGSHNHPKPQSTKRSSSTAIAAHQNSSNGDGKDIGED 204 >At4g17520.1 68417.m02621 nuclear RNA-binding protein, putative similar to nuclear RNA binding protein GI:6492264 from [Arabidopsis thaliana] Length = 360 Score = 27.9 bits (59), Expect = 7.2 Identities = 15/44 (34%), Positives = 21/44 (47%) Frame = -2 Query: 216 GSHHHRAEEGTRRRQGRAKGAHRTDRSRSQGDQGPERGNPGCRK 85 G + R+EEG R+G G +R DR S + G + RK Sbjct: 95 GGGYRRSEEGDGARRGGPVGGYRGDRRGSYSNGGDSGDSERPRK 138 >At1g76770.1 68414.m08934 heat shock protein-related contains similarity to 17.9 kDa heat-shock protein [Helianthus annuus] gi|11990130|emb|CAB55634 Length = 244 Score = 27.9 bits (59), Expect = 7.2 Identities = 11/37 (29%), Positives = 24/37 (64%), Gaps = 1/37 (2%) Frame = -3 Query: 293 RQFVRRYALPEGAAPETVESRLSS-DGVLTITAPRKV 186 ++F + + +P+ + +++R + DG LT+T P+KV Sbjct: 93 KEFKKVFRIPDIVILDKIKARFNEEDGTLTVTMPKKV 129 >At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containing protein low similarity to fertility restorer [Petunia x hybrida] GI:22128587, post-transcriptional control of chloroplast gene expression CRP1 [Zea mays] GI:3289002; contains Pfam profile PF01535: PPR repeat Length = 991 Score = 27.9 bits (59), Expect = 7.2 Identities = 10/27 (37%), Positives = 15/27 (55%) Frame = +3 Query: 141 GLCDGHLSLSLDGVGYLPRRGDGENPV 221 G C G + L LD + P+ G ++PV Sbjct: 240 GWCAGKVDLDLDSIDDFPKNGSAQSPV 266 >At1g62570.1 68414.m07059 flavin-containing monooxygenase family protein / FMO family protein low similarity to flavin-containing monooxygenase FMO3 [Rattus norvegicus] GI:12006730; contains Pfam profile PF00743: Flavin-binding monooxygenase-like Length = 461 Score = 27.9 bits (59), Expect = 7.2 Identities = 15/33 (45%), Positives = 18/33 (54%) Frame = -1 Query: 235 RDCHQTGFSPSPRRGRYPTPSRESERCPSHRPV 137 R+C G+ P R PSR+S R PSHR V Sbjct: 83 REC--MGYKDFPFVPRGDDPSRDSRRYPSHREV 113 >At1g28060.1 68414.m03435 small nuclear ribonucleoprotein family protein / snRNP family protein contains similarity to U4/U6 small nuclear ribonucleoprotein hPrp3 [Homo sapiens] gi|2708307|gb|AAC51926 Length = 786 Score = 27.9 bits (59), Expect = 7.2 Identities = 12/33 (36%), Positives = 18/33 (54%) Frame = -2 Query: 222 RRGSHHHRAEEGTRRRQGRAKGAHRTDRSRSQG 124 R HH R+ EG++ ++ R+K R DR G Sbjct: 80 REKHHHERSHEGSKEKESRSK---RKDREEENG 109 >At5g18190.1 68418.m02135 protein kinase family protein contains Pfam domains, PF00069: Protein kinase domain Length = 691 Score = 27.5 bits (58), Expect = 9.6 Identities = 11/22 (50%), Positives = 11/22 (50%) Frame = -2 Query: 486 QTMETTRGCIS*PRFQHKGRQG 421 Q T GC P HKGRQG Sbjct: 185 QVYNTLNGCYGVPAVHHKGRQG 206 >At3g56100.1 68416.m06235 leucine-rich repeat transmembrane protein kinase, putative hypothetical proteins - Arabidopsis thaliana Length = 719 Score = 27.5 bits (58), Expect = 9.6 Identities = 13/25 (52%), Positives = 15/25 (60%) Frame = -1 Query: 217 GFSPSPRRGRYPTPSRESERCPSHR 143 G+S S P+PS E ER PSHR Sbjct: 342 GYSVSTPCPTLPSPSPEKERKPSHR 366 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,563,246 Number of Sequences: 28952 Number of extensions: 359501 Number of successful extensions: 1199 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 1144 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1199 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1584903024 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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