Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner10f06f
(588 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g31300.1 68417.m04441 20S proteasome beta subunit A (PBA1) (P... 158 3e-39
At5g40580.2 68418.m04925 20S proteasome beta subunit B (PBB2) (P... 86 2e-17
At5g40580.1 68418.m04924 20S proteasome beta subunit B (PBB2) (P... 86 2e-17
At3g27430.2 68416.m03429 20S proteasome beta subunit B (PBB1) id... 85 3e-17
At3g27430.1 68416.m03428 20S proteasome beta subunit B (PBB1) id... 85 3e-17
At1g13060.1 68414.m01514 20S proteasome beta subunit E1 (PBE1) (... 64 7e-11
At3g26340.1 68416.m03286 20S proteasome beta subunit E, putative... 58 6e-09
At3g22110.1 68416.m02791 20S proteasome alpha subunit C (PAC1) (... 40 0.001
At4g14800.1 68417.m02275 20S proteasome beta subunit D2 (PBD2) (... 39 0.003
At3g60820.1 68416.m06804 20S proteasome beta subunit F1 (PBF1) 37 0.009
At4g02570.1 68417.m00351 cullin family protein similar to cullin... 28 5.3
At2g24450.1 68415.m02922 fasciclin-like arabinogalactan family p... 28 5.3
>At4g31300.1 68417.m04441 20S proteasome beta subunit A (PBA1)
(PRCD) identical to cDNA proteasome subunit prcd
GI:2511593
Length = 233
Score = 158 bits (383), Expect = 3e-39
Identities = 67/131 (51%), Positives = 96/131 (73%)
Frame = +1
Query: 145 MSAPHSTGTSIMACEFDGGVVIGADSRTTTGAYIANRVTDKLTKITDQIYCCRSGSAADT 324
+ APHS GT+I+ ++GGVV+GADSRT+TG Y+ANR +DK+T++TD +Y CRSGSAAD+
Sbjct: 5 LDAPHSMGTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRSGSAADS 64
Query: 325 QAIADIVTYHLNFHKMELGDPPLVETAAAIFRELCYNYRDSLMAGILVAGWDKKKGAQIY 504
Q ++D V Y L+ H ++ G P V+ +A + R L YN ++ L G++V GWDK +G +IY
Sbjct: 65 QVVSDYVRYFLHQHTIQHGQPATVKVSANLIRMLAYNNKNMLQTGLIVGGWDKYEGGKIY 124
Query: 505 SIPIGGMVQRQ 537
IP+GG V Q
Sbjct: 125 GIPLGGTVVEQ 135
>At5g40580.2 68418.m04925 20S proteasome beta subunit B (PBB2)
(PRCFC) identical to 20S proteasome beta subunit PBB2
[Arabidopsis thaliana] GI:3421104, cDNA proteasome
subunit prcfc GI:2511575
Length = 274
Score = 85.8 bits (203), Expect = 2e-17
Identities = 37/106 (34%), Positives = 66/106 (62%)
Frame = +1
Query: 163 TGTSIMACEFDGGVVIGADSRTTTGAYIANRVTDKLTKITDQIYCCRSGSAADTQAIADI 342
TGT+I+ F GV++GAD+R T G +A++ +K+ + IYCC +G+AADT+A+ D+
Sbjct: 38 TGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDM 97
Query: 343 VTYHLNFHKMELGDPPLVETAAAIFRELCYNYRDSLMAGILVAGWD 480
V+ L H+ + G V TA + ++ ++Y+ + A +++ G D
Sbjct: 98 VSSQLRLHRYQTGRDSRVVTALTLLKKHLFSYQGHVSAALVLGGVD 143
>At5g40580.1 68418.m04924 20S proteasome beta subunit B (PBB2)
(PRCFC) identical to 20S proteasome beta subunit PBB2
[Arabidopsis thaliana] GI:3421104, cDNA proteasome
subunit prcfc GI:2511575
Length = 274
Score = 85.8 bits (203), Expect = 2e-17
Identities = 37/106 (34%), Positives = 66/106 (62%)
Frame = +1
Query: 163 TGTSIMACEFDGGVVIGADSRTTTGAYIANRVTDKLTKITDQIYCCRSGSAADTQAIADI 342
TGT+I+ F GV++GAD+R T G +A++ +K+ + IYCC +G+AADT+A+ D+
Sbjct: 38 TGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDM 97
Query: 343 VTYHLNFHKMELGDPPLVETAAAIFRELCYNYRDSLMAGILVAGWD 480
V+ L H+ + G V TA + ++ ++Y+ + A +++ G D
Sbjct: 98 VSSQLRLHRYQTGRDSRVVTALTLLKKHLFSYQGHVSAALVLGGVD 143
>At3g27430.2 68416.m03429 20S proteasome beta subunit B (PBB1)
identical to 20S proteasome beta subunit PBB1 (PBB1)
GB:AAC32066 [Arabidopsis thaliana] (Genetics 149 (2),
677-692 (1998)); contains Pfam profile: PF00227
proteasome A-type and B-type;
Length = 273
Score = 85.4 bits (202), Expect = 3e-17
Identities = 37/106 (34%), Positives = 66/106 (62%)
Frame = +1
Query: 163 TGTSIMACEFDGGVVIGADSRTTTGAYIANRVTDKLTKITDQIYCCRSGSAADTQAIADI 342
TGT+I+ F GV++GAD+R T G +A++ +K+ + IYCC +G+AADT+A+ D+
Sbjct: 38 TGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDM 97
Query: 343 VTYHLNFHKMELGDPPLVETAAAIFRELCYNYRDSLMAGILVAGWD 480
V+ L H+ + G V TA + ++ ++Y+ + A +++ G D
Sbjct: 98 VSSQLRLHRYQTGRDSRVITALTLLKKHLFSYQGHVSAALVLGGVD 143
>At3g27430.1 68416.m03428 20S proteasome beta subunit B (PBB1)
identical to 20S proteasome beta subunit PBB1 (PBB1)
GB:AAC32066 [Arabidopsis thaliana] (Genetics 149 (2),
677-692 (1998)); contains Pfam profile: PF00227
proteasome A-type and B-type;
Length = 267
Score = 85.4 bits (202), Expect = 3e-17
Identities = 37/106 (34%), Positives = 66/106 (62%)
Frame = +1
Query: 163 TGTSIMACEFDGGVVIGADSRTTTGAYIANRVTDKLTKITDQIYCCRSGSAADTQAIADI 342
TGT+I+ F GV++GAD+R T G +A++ +K+ + IYCC +G+AADT+A+ D+
Sbjct: 38 TGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDM 97
Query: 343 VTYHLNFHKMELGDPPLVETAAAIFRELCYNYRDSLMAGILVAGWD 480
V+ L H+ + G V TA + ++ ++Y+ + A +++ G D
Sbjct: 98 VSSQLRLHRYQTGRDSRVITALTLLKKHLFSYQGHVSAALVLGGVD 143
>At1g13060.1 68414.m01514 20S proteasome beta subunit E1 (PBE1)
(PRCE) identical to GB:O23717; identical to cDNA
proteasome subunit prce GI:2511595
Length = 274
Score = 64.1 bits (149), Expect = 7e-11
Identities = 37/126 (29%), Positives = 62/126 (49%), Gaps = 1/126 (0%)
Frame = +1
Query: 157 HSTGTSIMACEFDGGVVIGADSRTTTGAYIANRVTDKLTKITDQIYCCRSGSAADTQAIA 336
H+ GT+ +A F GGV++ ADSR + G YI+++ K+ +I + +G AAD Q
Sbjct: 54 HAKGTTTLAFIFKGGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWH 113
Query: 337 DIVTYHLNFHKMELGDPPLVETAAAIFRELCYNYRD-SLMAGILVAGWDKKKGAQIYSIP 513
+ H++ V A+ + + Y+YR L G ++AGWD+ Y
Sbjct: 114 RNLGIKCRLHELANKRRISVSGASKLLANMLYSYRGMGLSVGTMIAGWDETGPGLYYVDN 173
Query: 514 IGGMVQ 531
GG ++
Sbjct: 174 EGGRLK 179
>At3g26340.1 68416.m03286 20S proteasome beta subunit E, putative
very strong similarity to SP|O23717 Proteasome subunit
beta type 5 precursor (EC 3.4.25.1) (20S proteasome
subunit E) (Proteasome epsilon chain) {Arabidopsis
thaliana}
Length = 273
Score = 57.6 bits (133), Expect = 6e-09
Identities = 35/123 (28%), Positives = 59/123 (47%), Gaps = 1/123 (0%)
Frame = +1
Query: 166 GTSIMACEFDGGVVIGADSRTTTGAYIANRVTDKLTKITDQIYCCRSGSAADTQAIADIV 345
GT+ +A F GV++ ADSR + G YI+++ K+ +I + +G AAD Q +
Sbjct: 57 GTTTLAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNL 116
Query: 346 TYHLNFHKMELGDPPLVETAAAIFRELCYNYRD-SLMAGILVAGWDKKKGAQIYSIPIGG 522
H++ V A+ + + Y+YR L G ++AGWD+ Y GG
Sbjct: 117 GIKCRLHELANKRRISVSGASKLLANMLYSYRGMGLSVGTMIAGWDETGPGLYYVDNEGG 176
Query: 523 MVQ 531
++
Sbjct: 177 RLK 179
>At3g22110.1 68416.m02791 20S proteasome alpha subunit C (PAC1)
(PRC9) identical to GB:AAC32057 from [Arabidopsis
thaliana] (Genetics (1998) 149 (2), 677-692); identical
to cDNA proteasome subunit prc9 GI:2511583
Length = 250
Score = 39.9 bits (89), Expect = 0.001
Identities = 33/131 (25%), Positives = 52/131 (39%), Gaps = 6/131 (4%)
Frame = +1
Query: 145 MSAPHSTGTSIMACEFDGGVVIGADSRTTTGAYIANRVTDKLTKITDQIYCCRSGSAADT 324
M A + G++I DG V+IG + + T+ + +K+ KI D + C +G +D
Sbjct: 25 MEAIGNAGSAIGILSKDGVVLIG-EKKVTSKLLQTSTSAEKMYKIDDHVACAVAGIMSDA 83
Query: 325 QAIADIVTYHLNFHKMELGDPPLVETAAAIFRELCYNYRD-----SLMAGILVAGWDKKK 489
+ + + +P VE + Y L AGWDK
Sbjct: 84 NILINTARVQAQRYTFMYQEPMPVEQLVQSLCDTKQGYTQFGGLRPFGVSFLFAGWDKHH 143
Query: 490 GAQIY-SIPIG 519
G Q+Y S P G
Sbjct: 144 GFQLYMSDPSG 154
>At4g14800.1 68417.m02275 20S proteasome beta subunit D2 (PBD2)
(PRCGA) identical to SP|O24633 Proteasome subunit beta
type 2-2 (EC 3.4.25.1) (20S proteasome alpha subunit D2)
{Arabidopsis thaliana}, cDNA proteasome subunit prcga
GI:2511571
Length = 199
Score = 38.7 bits (86), Expect = 0.003
Identities = 27/108 (25%), Positives = 45/108 (41%), Gaps = 2/108 (1%)
Frame = +1
Query: 193 DGGVVIGADSRTTTGAYIANRVTDKLTKITDQIYCCRSGSAADTQAIADIVTYHLNFHKM 372
+G ++ AD+ + DK+ + SG D + V +++ ++
Sbjct: 10 NGFAIVAADTSAVHSILLHKNKEDKIMTLDSHKLVAASGEPGDRVQFTEYVQKNVSLYQF 69
Query: 373 ELGDPPLVETAAAIFR-ELCYNYRDS-LMAGILVAGWDKKKGAQIYSI 510
G P AA R EL R + IL+AG+DK+ GA +Y I
Sbjct: 70 RNGIPLSTAAAANFTRGELATALRKNPYSVNILMAGYDKEAGASLYYI 117
>At3g60820.1 68416.m06804 20S proteasome beta subunit F1 (PBF1)
Length = 223
Score = 37.1 bits (82), Expect = 0.009
Identities = 25/90 (27%), Positives = 44/90 (48%), Gaps = 4/90 (4%)
Frame = +1
Query: 142 WMSAPHSTGTSIMACEFDGGVVIGADSRTTTGAYIANRVTDKLTKITDQIYCCRSGSAAD 321
W ++ GT + D VI AD+R +TG I +R K+ K+ D+ SG AD
Sbjct: 8 WSPYDNNGGTCVAIAGSDY-CVIAADTRMSTGYSILSRDYSKIHKLADRAVLSSSGFQAD 66
Query: 322 TQAIADIV-TYHLNF---HKMELGDPPLVE 399
+A+ ++ + HL + H ++ P + +
Sbjct: 67 VKALQKVLKSRHLIYQHQHNKQMSCPAMAQ 96
>At4g02570.1 68417.m00351 cullin family protein similar to cullin 3
[Homo sapiens] GI:3639052; contains Pfam profile
PF00888: Cullin family
Length = 738
Score = 27.9 bits (59), Expect = 5.3
Identities = 16/40 (40%), Positives = 22/40 (55%), Gaps = 1/40 (2%)
Frame = +1
Query: 385 PPLVETAAAIFRELCYNYRDSLMAGILVAGWDK-KKGAQI 501
PPL E FR+L YN S + ++A DK ++G QI
Sbjct: 126 PPLNEVGLTCFRDLVYNELHSKVKQAVIALVDKEREGEQI 165
>At2g24450.1 68415.m02922 fasciclin-like arabinogalactan family
protein similar to fasciclin-like
arabinogalactan-protein 1 [Arabidopsis thaliana]
gi|13377776|gb|AAK20857
Length = 280
Score = 27.9 bits (59), Expect = 5.3
Identities = 13/39 (33%), Positives = 22/39 (56%)
Frame = -2
Query: 302 RQQYIWSVIFVSLSVTRLAM*APVVVRESAPMTTPPSNS 186
R Y SV+ +S+ + + +PV V PM++PP+ S
Sbjct: 155 RNPYNLSVVQISMPIVAPGLGSPVKVPPPPPMSSPPAPS 193
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,591,614
Number of Sequences: 28952
Number of extensions: 227170
Number of successful extensions: 499
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 488
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 497
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1161268208
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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