Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner10e17r
(750 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g08570.1 68418.m01020 pyruvate kinase, putative similar to py... 50 1e-06
At3g55810.1 68416.m06201 pyruvate kinase, putative similar to py... 49 4e-06
At3g25960.1 68416.m03235 pyruvate kinase, putative similar to py... 48 9e-06
At3g22960.1 68416.m02895 pyruvate kinase, putative similar to py... 48 9e-06
At3g04050.1 68416.m00427 pyruvate kinase, putative similar to py... 48 9e-06
At5g63680.1 68418.m07994 pyruvate kinase, putative similar to py... 47 2e-05
At1g32440.1 68414.m04004 pyruvate kinase, putative similar to py... 46 2e-05
At4g26390.1 68417.m03797 pyruvate kinase, putative identical to ... 46 3e-05
At3g55650.1 68416.m06183 pyruvate kinase, putative simlar to pyr... 46 3e-05
At5g56350.1 68418.m07033 pyruvate kinase, putative similar to py... 45 6e-05
At2g36580.1 68415.m04486 pyruvate kinase, putative similar to py... 44 8e-05
At5g52920.1 68418.m06567 pyruvate kinase, putative similar to py... 43 3e-04
At3g52990.1 68416.m05841 pyruvate kinase, putative similar to py... 42 4e-04
>At5g08570.1 68418.m01020 pyruvate kinase, putative similar to
pyruvate kinase, cytosolic isozyme [Glycine max]
SWISS-PROT:Q42806
Length = 510
Score = 50.4 bits (115), Expect = 1e-06
Identities = 33/135 (24%), Positives = 62/135 (45%), Gaps = 13/135 (9%)
Frame = -1
Query: 744 HRQLFNDLVSEVKPPIDPXXXXXXXXXXXATKCLASAIVVITTSGKSAHLLSKYRPRCPI 565
+ +F +++ P+ P A K A I+V+T G +A+L++KYRP PI
Sbjct: 361 YNTIFKEMIRATPLPMSPLESLASSAVRTANKARAKLIIVLTRGGSTANLVAKYRPAVPI 420
Query: 564 IAVTRHPQT-------------ARQVHLYRGVLPIVYQEPTASDWLKDVDNRVQSGLRFG 424
++V T AR +YRG++P++ + + + + +++ L+
Sbjct: 421 LSVVVPVMTTDSFDWSCSDESPARHSLIYRGLIPMLAEGSAKATDSEATEVIIEAALKSA 480
Query: 423 RQRGFVHPGDNAVVV 379
QRG + GD V +
Sbjct: 481 TQRGLCNRGDAIVAL 495
>At3g55810.1 68416.m06201 pyruvate kinase, putative similar to
pyruvate kinase, cytosolic isozyme [Nicotiana tabacum]
SWISS-PROT:Q42954
Length = 492
Score = 48.8 bits (111), Expect = 4e-06
Identities = 36/125 (28%), Positives = 56/125 (44%), Gaps = 17/125 (13%)
Frame = -1
Query: 702 PIDPXXXXXXXXXXXATKCLASAIVVITTSGKSAHLLSKYRPRCPIIAV----------- 556
P+ P A ASAIVV+T G +A L++KYRP PI++V
Sbjct: 353 PLSPIESLAASVVSTAQSVFASAIVVLTKGGYTAELVAKYRPSVPILSVIVPEIAQGNDI 412
Query: 555 -----TRHPQTARQVHLYRGVLPIVYQEPTASDWLKD-VDNRVQSGLRFGRQRGFVHPGD 394
AR+ +YRG++P+V +A D KD + + + F + +G GD
Sbjct: 413 EMSCSDSVAHVARRGLIYRGIIPVVATGSSARDSNKDATEEMINLAIGFAKTKGICKNGD 472
Query: 393 NAVVV 379
+ V +
Sbjct: 473 SIVAL 477
>At3g25960.1 68416.m03235 pyruvate kinase, putative similar to
pyruvate kinase, cytosolic isozyme [Nicotiana tabacum]
SWISS-PROT:Q42954
Length = 497
Score = 47.6 bits (108), Expect = 9e-06
Identities = 35/120 (29%), Positives = 56/120 (46%), Gaps = 9/120 (7%)
Frame = -1
Query: 711 VKPPIDPXXXXXXXXXXXATKCLASAIVVITTSGKSAHLLSKYRPRCPIIAVTRH----- 547
V P+ P A ASAIVV+T G +A L++KYRP PI++V
Sbjct: 368 VSLPLSPIESLAASAVSTARSVFASAIVVLTRGGYTAELVAKYRPSVPILSVIMPEIAEC 427
Query: 546 ----PQTARQVHLYRGVLPIVYQEPTASDWLKDVDNRVQSGLRFGRQRGFVHPGDNAVVV 379
AR+ +YRG++P+V +A D + ++ + F + +G GD+ V +
Sbjct: 428 SDSVAHVARRGLIYRGIIPVV--GCSARD---STEEMIRLAIGFAKTKGICKTGDSIVAL 482
>At3g22960.1 68416.m02895 pyruvate kinase, putative similar to
pyruvate kinase isozyme A, chloroplast precursor
[Ricinus communis] SWISS-PROT:Q43117
Length = 596
Score = 47.6 bits (108), Expect = 9e-06
Identities = 29/91 (31%), Positives = 46/91 (50%)
Frame = -1
Query: 636 AIVVITTSGKSAHLLSKYRPRCPIIAVTRHPQTARQVHLYRGVLPIVYQEPTASDWLKDV 457
A+ V TTSG A L+S+ RP CPI A T R+++L G++P + SD D+
Sbjct: 504 AVFVYTTSGHMASLVSRCRPDCPIFAFTTTTSVRRRLNLQWGLIPF---RLSFSD---DM 557
Query: 456 DNRVQSGLRFGRQRGFVHPGDNAVVVTGWKQ 364
++ + + RG + GD + V+ Q
Sbjct: 558 ESNLNKTFSLLKSRGMIKSGDLVIAVSDMLQ 588
>At3g04050.1 68416.m00427 pyruvate kinase, putative similar to
pyruvate kinase, cytosolic isozyme [Nicotiana tabacum]
SWISS-PROT:Q42954
Length = 510
Score = 47.6 bits (108), Expect = 9e-06
Identities = 36/128 (28%), Positives = 59/128 (46%), Gaps = 17/128 (13%)
Frame = -1
Query: 711 VKPPIDPXXXXXXXXXXXATKCLASAIVVITTSGKSAHLLSKYRPRCPIIAV-----TR- 550
V P+ P A A+AIVV+T G + L++KYRP PI++V TR
Sbjct: 368 VSLPLSPIESLAASAVSTARSLCAAAIVVLTKGGYTVELVAKYRPSVPILSVIVPEITRT 427
Query: 549 ----------HPQTARQVHLYRGVLPIVYQEPTASDWLKD-VDNRVQSGLRFGRQRGFVH 403
AR+ +YRG++P++ +A KD + +Q + F +++G
Sbjct: 428 DDFEWSCSESAAHVARRGLIYRGIVPVMATGASARSSNKDSTEETIQFAIEFAKKKGICK 487
Query: 402 PGDNAVVV 379
GD+ V +
Sbjct: 488 TGDSIVAL 495
>At5g63680.1 68418.m07994 pyruvate kinase, putative similar to
pyruvate kinase, cytosolic isozyme [Glycine max]
SWISS-PROT:Q42806
Length = 510
Score = 46.8 bits (106), Expect = 2e-05
Identities = 30/135 (22%), Positives = 59/135 (43%), Gaps = 13/135 (9%)
Frame = -1
Query: 744 HRQLFNDLVSEVKPPIDPXXXXXXXXXXXATKCLASAIVVITTSGKSAHLLSKYRPRCPI 565
+ +F +++ P+ A K A I+V+T G +A L++KYRP PI
Sbjct: 361 YNTIFKEMIRATPLPMSTLESLASSAVRTANKAKAKLIIVLTRGGTTAKLVAKYRPAVPI 420
Query: 564 IAVT-------------RHPQTARQVHLYRGVLPIVYQEPTASDWLKDVDNRVQSGLRFG 424
++V AR +YRG++P++ + + + + ++S L+
Sbjct: 421 LSVVVPVFTSDTFNWSCSDESPARHSLIYRGLIPVLGEGSAKATDSESTEEIIESALKSA 480
Query: 423 RQRGFVHPGDNAVVV 379
++G + GD V +
Sbjct: 481 TEKGLCNHGDAVVAL 495
>At1g32440.1 68414.m04004 pyruvate kinase, putative similar to
pyruvate kinase isozyme G, chloroplast precursor
[Nicotiana tabacum] SWISS-PROT:Q40546
Length = 571
Score = 46.4 bits (105), Expect = 2e-05
Identities = 32/107 (29%), Positives = 52/107 (48%)
Frame = -1
Query: 645 LASAIVVITTSGKSAHLLSKYRPRCPIIAVTRHPQTARQVHLYRGVLPIVYQEPTASDWL 466
L+S ++V T +G A LLS YRP I A T + +++ LY+GV+PI Y E SD
Sbjct: 464 LSSPLIVFTRTGSMAVLLSHYRPSATIFAFTNQRRIMQRLALYQGVMPI-YME--FSD-- 518
Query: 465 KDVDNRVQSGLRFGRQRGFVHPGDNAVVVTGWKQGSGFTNTVRVIQL 325
D ++ L+ + + G + +V Q + +IQ+
Sbjct: 519 -DAEDTYARSLKLLQDENMLKEGQHVTLVQSGSQPIWREESTHLIQV 564
>At4g26390.1 68417.m03797 pyruvate kinase, putative identical to
probable pyruvate kinase, cytosolic isozyme (EC
2.7.1.40) [Arabidopsis thaliana] SWISS-PROT:O65595
Length = 497
Score = 46.0 bits (104), Expect = 3e-05
Identities = 29/132 (21%), Positives = 59/132 (44%), Gaps = 13/132 (9%)
Frame = -1
Query: 735 LFNDLVSEVKPPIDPXXXXXXXXXXXATKCLASAIVVITTSGKSAHLLSKYRPRCPIIAV 556
+F ++ P+ P AT A+ ++V+T G +A L++KYRP PI++V
Sbjct: 351 IFKRIMLHAAVPMSPMESLASSAVRTATSSRATLMMVLTRGGSTARLVAKYRPGIPILSV 410
Query: 555 T-------------RHPQTARQVHLYRGVLPIVYQEPTASDWLKDVDNRVQSGLRFGRQR 415
+ AR +YRG++P++Y + + + ++ +G+++
Sbjct: 411 VVPEITSDSFDWACSNEAPARHSLIYRGLVPVLYAGSARASIDESTEETLEFASEYGKKK 470
Query: 414 GFVHPGDNAVVV 379
GD+ V +
Sbjct: 471 QLCKTGDSVVAL 482
>At3g55650.1 68416.m06183 pyruvate kinase, putative simlar to
pyruvate kinase, cytosolic isozyme [Nicotiana tabacum]
SWISS-PROT:Q42954
Length = 510
Score = 46.0 bits (104), Expect = 3e-05
Identities = 36/128 (28%), Positives = 56/128 (43%), Gaps = 17/128 (13%)
Frame = -1
Query: 711 VKPPIDPXXXXXXXXXXXATKCLASAIVVITTSGKSAHLLSKYRPRCPIIAV-------- 556
V P+ P A ASAIVV+T G +A L++KYRP PI++V
Sbjct: 368 VSLPLSPIESLAASVVSTAQSVFASAIVVLTKGGYTAELVAKYRPSVPILSVIVPEIAQG 427
Query: 555 --------TRHPQTARQVHLYRGVLPIVYQEPTASDWLKD-VDNRVQSGLRFGRQRGFVH 403
AR+ +YR ++P+V +A D KD + + + F + +G
Sbjct: 428 NDMEMSCSDSVAHAARRGLIYRRIIPVVATGSSARDSNKDATEEMINLAIGFAKTKGICK 487
Query: 402 PGDNAVVV 379
GD+ V +
Sbjct: 488 NGDSIVAL 495
>At5g56350.1 68418.m07033 pyruvate kinase, putative similar to
pyruvate kinase, cytosolic isozyme [Nicotiana tabacum]
SWISS-PROT:Q42954
Length = 498
Score = 44.8 bits (101), Expect = 6e-05
Identities = 27/121 (22%), Positives = 54/121 (44%), Gaps = 13/121 (10%)
Frame = -1
Query: 702 PIDPXXXXXXXXXXXATKCLASAIVVITTSGKSAHLLSKYRPRCPIIAVT---------- 553
P+ P A A+ I+V+T G +A L++KYRP PI++V
Sbjct: 363 PMSPLESLASSAVRTANSARATLIMVLTRGGSTARLVAKYRPGMPILSVVVPEIKTDFFD 422
Query: 552 ---RHPQTARQVHLYRGVLPIVYQEPTASDWLKDVDNRVQSGLRFGRQRGFVHPGDNAVV 382
AR ++RG++P++Y + + + ++ ++G+++ GD+ V
Sbjct: 423 WSCSDESPARHSLIFRGLIPVLYAGSARASHDESTEEAIEFATQYGKEKELCKTGDSVVA 482
Query: 381 V 379
+
Sbjct: 483 L 483
>At2g36580.1 68415.m04486 pyruvate kinase, putative similar to
pyruvate kinase, cytosolic isozyme [Glycine max]
SWISS-PROT:Q42806
Length = 527
Score = 44.4 bits (100), Expect = 8e-05
Identities = 36/124 (29%), Positives = 59/124 (47%), Gaps = 14/124 (11%)
Frame = -1
Query: 651 KCLASAIVVITTSGKSAHLLSKYRPRCPIIAVTRHPQT-------------ARQVHLYRG 511
K AS I+ T+SG++A L++KYRP P+++V T ARQ + RG
Sbjct: 407 KVKASVIICFTSSGRAARLIAKYRPTMPVLSVVIPRLTTNQLKWSFSGAFEARQSLIVRG 466
Query: 510 VLPIVYQEPTASDWLKDVDNRV-QSGLRFGRQRGFVHPGDNAVVVTGWKQGSGFTNTVRV 334
+ P++ ++ + V + L G+Q G + D VV Q G + V++
Sbjct: 467 LFPMLADPRHPAESTSATNESVLKVALDHGKQAGVIKSHDRVVVC----QKVGDASVVKI 522
Query: 333 IQLE 322
I+LE
Sbjct: 523 IELE 526
>At5g52920.1 68418.m06567 pyruvate kinase, putative similar to
pyruvate kinase isozyme G, chloroplast precursor
[Nicotiana tabacum] SWISS-PROT:Q40546
Length = 579
Score = 42.7 bits (96), Expect = 3e-04
Identities = 31/94 (32%), Positives = 45/94 (47%)
Frame = -1
Query: 645 LASAIVVITTSGKSAHLLSKYRPRCPIIAVTRHPQTARQVHLYRGVLPIVYQEPTASDWL 466
L ++ VV T +G A LLS YRP I A T + +++ LY+GV PI Y E T
Sbjct: 476 LGTSTVVFTRTGFMAILLSHYRPSGTIYAFTNEKKIQQRLALYQGVCPI-YMEFT----- 529
Query: 465 KDVDNRVQSGLRFGRQRGFVHPGDNAVVVTGWKQ 364
D + + L ++G V G+ +V Q
Sbjct: 530 DDAEETFANALATLLKQGMVKKGEEIAIVQSGTQ 563
>At3g52990.1 68416.m05841 pyruvate kinase, putative similar to
pyruvate kinase, cytosolic isozyme [Glycine max]
SWISS-PROT:Q42806
Length = 527
Score = 41.9 bits (94), Expect = 4e-04
Identities = 36/124 (29%), Positives = 59/124 (47%), Gaps = 14/124 (11%)
Frame = -1
Query: 651 KCLASAIVVITTSGKSAHLLSKYRPRCPIIAV----TRHPQ---------TARQVHLYRG 511
K AS I+ T+SG++A L++KYRP P+I+V + Q ARQ + RG
Sbjct: 407 KVKASVIICFTSSGRAARLIAKYRPTMPVISVVIPRVKTNQLKWSFSGAFEARQSLIVRG 466
Query: 510 VLPIVYQEPTASDWLKDVDNRV-QSGLRFGRQRGFVHPGDNAVVVTGWKQGSGFTNTVRV 334
+ P++ ++ + V + L G+ G + D VV Q G + V++
Sbjct: 467 LFPMLADPRHPAESTSATNESVLKVALDHGKHAGVIKSHDRVVVC----QKVGDASVVKI 522
Query: 333 IQLE 322
I+LE
Sbjct: 523 IELE 526
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,730,979
Number of Sequences: 28952
Number of extensions: 241783
Number of successful extensions: 559
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 537
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 557
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1663169840
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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