BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fner10e17f (585 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC4H3.10c |pyk1||pyruvate kinase |Schizosaccharomyces pombe|ch... 101 8e-23 SPAC25G10.04c |rec10|rec20|meiotic recombination protein Rec10 |... 27 2.0 SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated prote... 27 2.7 SPAC17G8.12 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 27 2.7 SPCC285.13c |||nucleoporin Nup60 |Schizosaccharomyces pombe|chr ... 27 2.7 SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual 26 4.7 SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 26 4.7 SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 25 8.1 SPAC1705.03c ||SPAC23H4.19|conserved fungal family|Schizosacchar... 25 8.1 >SPAC4H3.10c |pyk1||pyruvate kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 509 Score = 101 bits (242), Expect = 8e-23 Identities = 54/106 (50%), Positives = 64/106 (60%) Frame = +1 Query: 268 RLSGIICTIGPASRNVAVLEKMMETGMNVARMNFSHGSHEYHAETIRNCREAEKSYSAKL 447 R + IICTIGP S NV L K+ + GMN+ RMNFSHGS+EYH I N R+A + Sbjct: 26 RRTSIICTIGPKSNNVETLCKLRDAGMNIVRMNFSHGSYEYHQSVIDNARKASAT----- 80 Query: 448 GSPFSLAIALDTKGPEIRTGLLEGGGSAEVELKKGETIKLTTSSDY 585 F LAIALDTKGPEIRTGL GG + + G + TT Y Sbjct: 81 NPLFPLAIALDTKGPEIRTGLTVGG--TDYPISSGHEMIFTTDDAY 124 >SPAC25G10.04c |rec10|rec20|meiotic recombination protein Rec10 |Schizosaccharomyces pombe|chr 1|||Manual Length = 791 Score = 27.1 bits (57), Expect = 2.0 Identities = 11/40 (27%), Positives = 22/40 (55%) Frame = +1 Query: 178 GAVEKPTVANVGSQLQHMCGLDIDSKSSYIRLSGIICTIG 297 G + + ++ L+ + D+D+K +YI+LS T+G Sbjct: 15 GTLNESSIYKTYQILESLNPKDVDTKENYIKLSNTFSTLG 54 >SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated protein Mug36|Schizosaccharomyces pombe|chr 2|||Manual Length = 1646 Score = 26.6 bits (56), Expect = 2.7 Identities = 25/119 (21%), Positives = 53/119 (44%), Gaps = 1/119 (0%) Frame = +1 Query: 133 LKMVYPTIH-DGEIEAGAVEKPTVANVGSQLQHMCGLDIDSKSSYIRLSGIICTIGPASR 309 LK+ + I+ + ++ G + T A + S ++ +C DS S ++S P ++ Sbjct: 1151 LKVKFKDINVNWDLHDGYDWEATRATISSAIEKLC----DSSSQNDKIS-------PEAK 1199 Query: 310 NVAVLEKMMETGMNVARMNFSHGSHEYHAETIRNCREAEKSYSAKLGSPFSLAIALDTK 486 + ++++ V R+N +H S ++ + SY +LG S I ++ K Sbjct: 1200 TLLFQSIVIKSFSKVGRLNINHVSEPIDSDEFADYLSKSISYHLRLGKSKSKKIGIEIK 1258 >SPAC17G8.12 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 608 Score = 26.6 bits (56), Expect = 2.7 Identities = 23/89 (25%), Positives = 36/89 (40%), Gaps = 4/89 (4%) Frame = -2 Query: 524 PP--PSKSPVLISGPLVSNAMAKEKGEPSLAL*LFSASRQLRMVSAWYSWEPCEKFILAT 351 PP PS+ P + + P + EPS+ L LR + + +P K L T Sbjct: 430 PPKHPSRQPTVRATPSTGKQIEPPNDEPSIGNELLPLFESLRFENRKTTSKPKAKKTLVT 489 Query: 350 FIPVSIIFSSTATLRDAGPIVQI--IPER 270 IP +S + D + + IPE+ Sbjct: 490 SIPTKPHQASVEKVYDLNSKINLKNIPEQ 518 >SPCC285.13c |||nucleoporin Nup60 |Schizosaccharomyces pombe|chr 3|||Manual Length = 736 Score = 26.6 bits (56), Expect = 2.7 Identities = 15/38 (39%), Positives = 21/38 (55%) Frame = -2 Query: 554 SPFFSSTSAEPPPSKSPVLISGPLVSNAMAKEKGEPSL 441 SP ST+A P K+P + L+S +KEK PS+ Sbjct: 305 SPLHQSTTANHP-EKTPSRAAASLLSILDSKEKNTPSI 341 >SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual Length = 4717 Score = 25.8 bits (54), Expect = 4.7 Identities = 12/29 (41%), Positives = 17/29 (58%) Frame = +3 Query: 240 RH*LQVFVHPSLRNNLYYWTGIP*CSCAR 326 RH L++ V L N+Y WT +P C +R Sbjct: 2344 RHFLKLVV---LWKNIYVWTNVPDCDISR 2369 >SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 513 Score = 25.8 bits (54), Expect = 4.7 Identities = 10/17 (58%), Positives = 11/17 (64%) Frame = -2 Query: 554 SPFFSSTSAEPPPSKSP 504 SP FSS+S PPP P Sbjct: 231 SPIFSSSSTPPPPPPRP 247 >SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosaccharomyces pombe|chr 1|||Manual Length = 157 Score = 25.0 bits (52), Expect = 8.1 Identities = 9/28 (32%), Positives = 16/28 (57%) Frame = +2 Query: 452 HPSLWPSHWTLKDLRSELDSWKAAAQLK 535 HP+++PS + +E + WK A +K Sbjct: 83 HPNVYPSGTVCLSILNEEEGWKPAITIK 110 >SPAC1705.03c ||SPAC23H4.19|conserved fungal family|Schizosaccharomyces pombe|chr 1|||Manual Length = 421 Score = 25.0 bits (52), Expect = 8.1 Identities = 11/41 (26%), Positives = 23/41 (56%) Frame = +1 Query: 142 VYPTIHDGEIEAGAVEKPTVANVGSQLQHMCGLDIDSKSSY 264 V P + + + AG + +V +QLQ + G+ +DS +++ Sbjct: 123 VLPNLQELQFNAGLSDSDSVVIDDTQLQAIDGISLDSVTTF 163 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.315 0.131 0.373 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,292,209 Number of Sequences: 5004 Number of extensions: 44423 Number of successful extensions: 122 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 117 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 121 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 252150250 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 42 (22.0 bits)
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