Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner10d17r
(775 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g20960.1 68414.m02624 U5 small nuclear ribonucleoprotein heli... 57 1e-08
At2g42270.1 68415.m05232 U5 small nuclear ribonucleoprotein heli... 54 1e-07
At4g21180.1 68417.m03063 DNAJ heat shock N-terminal domain-conta... 38 0.006
At4g15210.2 68417.m02331 beta-amylase (BMY1) / 1,4-alpha-D-gluca... 32 0.37
At4g15210.1 68417.m02330 beta-amylase (BMY1) / 1,4-alpha-D-gluca... 32 0.37
At5g61140.1 68418.m07670 DEAD box RNA helicase, putative similar... 32 0.49
At1g22630.1 68414.m02826 expressed protein contains Pfam PF00684... 32 0.49
At3g28870.1 68416.m03604 hypothetical protein 31 0.85
At1g79940.1 68414.m09342 DNAJ heat shock N-terminal domain-conta... 30 2.0
At4g10670.1 68417.m01743 transcription elongation factor-related... 29 3.4
>At1g20960.1 68414.m02624 U5 small nuclear ribonucleoprotein helicase,
putative similar to SP|O75643 U5 small nuclear
ribonucleoprotein 200 kDa helicase {Homo sapiens};
contains Pfam profiles PF00270: DEAD/DEAH box helicase,
PF00271: Helicase conserved C-terminal domain, PF02889:
Sec63 domain
Length = 2171
Score = 56.8 bits (131), Expect = 1e-08
Identities = 32/94 (34%), Positives = 50/94 (53%)
Frame = -1
Query: 493 LEGRSKSSHPVHCPYFPQEKQEYWWCYICDRKSHTLLTAPAHVTALVDTHELQLRFTAPR 314
+EGR++ PV +P+ K+E WW + D K++ LL +L +++L FTAP
Sbjct: 2075 MEGRTEVG-PVDSLRYPKTKEEGWWLVVGDTKTNQLLAIKR--VSLQRKVKVKLDFTAPS 2131
Query: 313 WPGLYTLACCLRSDSYIGMDQQQDMKLDVKEAAA 212
PG + DSY+G DQ+ +DVK + A
Sbjct: 2132 EPGEKSYTLYFMCDSYLGCDQEYSFSVDVKGSGA 2165
>At2g42270.1 68415.m05232 U5 small nuclear ribonucleoprotein helicase,
putative
Length = 2172
Score = 54.0 bits (124), Expect = 1e-07
Identities = 30/99 (30%), Positives = 52/99 (52%)
Frame = -1
Query: 493 LEGRSKSSHPVHCPYFPQEKQEYWWCYICDRKSHTLLTAPAHVTALVDTHELQLRFTAPR 314
+EGR++ PV P +P+ K+E WW + + K++ L+ +L +++L F P
Sbjct: 2076 MEGRTEVG-PVDAPRYPKTKEEGWWLVVGEAKTNQLMAIKR--ISLQRKAQVKLEFAVPT 2132
Query: 313 WPGLYTLACCLRSDSYIGMDQQQDMKLDVKEAAAVPAEH 197
G + DSY+G DQ+ +DVK++ A A+H
Sbjct: 2133 ETGEKSYTLYFMCDSYLGCDQEYSFTVDVKDSDA--ADH 2169
>At4g21180.1 68417.m03063 DNAJ heat shock N-terminal
domain-containing protein / sec63 domain-containing
protein similar to SP|Q9UGP8 Translocation protein SEC63
homolog {Homo sapiens}; contains Pfam profiles PF00226
DnaJ domain, PF02889 Sec63 domain
Length = 661
Score = 38.3 bits (85), Expect = 0.006
Identities = 24/68 (35%), Positives = 36/68 (52%), Gaps = 1/68 (1%)
Frame = -1
Query: 331 RFTAPRWPGLYTLACCLRSDSYIGMDQQQDMKLDV-KEAAAVPAEHPQWDLSDSDTDNND 155
R AP G Y L C SD++IG DQ+ +K++V K V E+ + L + D D +
Sbjct: 567 RLLAPG-EGTYNLTCFCLSDTWIGCDQKTSLKVEVLKRTRDVEGENAEEGLEEED-DEIE 624
Query: 154 QGGNESEF 131
+ ESE+
Sbjct: 625 EEDYESEY 632
Score = 27.9 bits (59), Expect = 7.9
Identities = 9/19 (47%), Positives = 12/19 (63%)
Frame = -1
Query: 460 HCPYFPQEKQEYWWCYICD 404
H PYFP K+E +W + D
Sbjct: 488 HSPYFPFHKEENFWVLLAD 506
>At4g15210.2 68417.m02331 beta-amylase (BMY1) / 1,4-alpha-D-glucan
maltohydrolase identical to Beta-amylase (EC 3.2.1.2)
(1,4-alpha-D-glucan maltohydrolase) SP:P25853
[Arabidopsis thaliana]
Length = 420
Score = 32.3 bits (70), Expect = 0.37
Identities = 15/30 (50%), Positives = 20/30 (66%)
Frame = -1
Query: 241 MKLDVKEAAAVPAEHPQWDLSDSDTDNNDQ 152
+K D KEAAA A HP+WDL + + ND+
Sbjct: 216 LKKDFKEAAA-KAGHPEWDLPEDAGEYNDK 244
>At4g15210.1 68417.m02330 beta-amylase (BMY1) / 1,4-alpha-D-glucan
maltohydrolase identical to Beta-amylase (EC 3.2.1.2)
(1,4-alpha-D-glucan maltohydrolase) SP:P25853
[Arabidopsis thaliana]
Length = 498
Score = 32.3 bits (70), Expect = 0.37
Identities = 15/30 (50%), Positives = 20/30 (66%)
Frame = -1
Query: 241 MKLDVKEAAAVPAEHPQWDLSDSDTDNNDQ 152
+K D KEAAA A HP+WDL + + ND+
Sbjct: 216 LKKDFKEAAA-KAGHPEWDLPEDAGEYNDK 244
>At5g61140.1 68418.m07670 DEAD box RNA helicase, putative similar to
ASC-1 complex subunit P200 [Homo sapiens] GI:12061185;
contains Pfam profiles PF00270: DEAD/DEAH box helicase,
PF00271: Helicase conserved C-terminal domain, PF02889:
Sec63 domain
Length = 2146
Score = 31.9 bits (69), Expect = 0.49
Identities = 26/89 (29%), Positives = 36/89 (40%), Gaps = 2/89 (2%)
Frame = -1
Query: 496 RLEGRSK-SSHPVHCPYFPQEKQEYWWCYICDRKSHTLLTAPAHVTALVDTHELQLRF-T 323
RLE SK +S P FP+ K E WW + D + L V + T L R
Sbjct: 2056 RLEKTSKRNSSRALAPRFPKVKDEAWWLVLGDTSTSELFA----VKRVSFTGRLITRMEL 2111
Query: 322 APRWPGLYTLACCLRSDSYIGMDQQQDMK 236
P L SD Y+G +Q+ ++
Sbjct: 2112 PPNITSFQDTKLILVSDCYLGFEQEHSIE 2140
>At1g22630.1 68414.m02826 expressed protein contains Pfam PF00684 :
DnaJ central domain (4 repeats)
Length = 110
Score = 31.9 bits (69), Expect = 0.49
Identities = 13/53 (24%), Positives = 26/53 (49%), Gaps = 2/53 (3%)
Frame = +3
Query: 318 GAVKRSCNSCVSTSAVTCAGAVSSVCDLRSQM*HHQYSCFSCGKYG--QCTGC 470
G++ +SC +C + A+ C G + + ++ ++ CF C +G C C
Sbjct: 45 GSIFKSCETCGAKGAIECPGCKGTGKNKKNGNMFERWKCFDCQGFGMKSCPKC 97
>At3g28870.1 68416.m03604 hypothetical protein
Length = 355
Score = 31.1 bits (67), Expect = 0.85
Identities = 14/40 (35%), Positives = 21/40 (52%)
Frame = -1
Query: 343 ELQLRFTAPRWPGLYTLACCLRSDSYIGMDQQQDMKLDVK 224
+++L FT P PG + DSY+G DQ+ +D K
Sbjct: 306 KVKLDFTVPSEPGEKSYTLYFMCDSYLGCDQEYSFSVDDK 345
>At1g79940.1 68414.m09342 DNAJ heat shock N-terminal
domain-containing protein / sec63 domain-containing
protein similar to SP|Q9UGP8 Translocation protein SEC63
homolog {Homo sapiens}; contains Pfam profiles PF00226
DnaJ domain, PF02889 Sec63 domain
Length = 702
Score = 29.9 bits (64), Expect = 2.0
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = -1
Query: 460 HCPYFPQEKQEYWWCYICDRKSHTL 386
H PYFP K+E +W + D S+ +
Sbjct: 507 HAPYFPFHKEENYWVLLADSVSNNV 531
Score = 27.9 bits (59), Expect = 7.9
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = -1
Query: 307 GLYTLACCLRSDSYIGMDQQQDMKLDV 227
G Y L C D++IG D++Q +K+ V
Sbjct: 595 GTYNLTCFCLCDTWIGCDKKQALKVKV 621
>At4g10670.1 68417.m01743 transcription elongation factor-related
low similarity to chromatin-specific transcription
elongation factor FACT 140 kDa subunit [Homo sapiens]
GI:5499741
Length = 470
Score = 29.1 bits (62), Expect = 3.4
Identities = 11/36 (30%), Positives = 20/36 (55%)
Frame = -1
Query: 247 QDMKLDVKEAAAVPAEHPQWDLSDSDTDNNDQGGNE 140
+D+K DV +VP W+ + D +++ GG+E
Sbjct: 328 KDLKKDVLRVDSVPTNAGGWEFLNQDGSDSESGGSE 363
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,978,972
Number of Sequences: 28952
Number of extensions: 160074
Number of successful extensions: 679
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 651
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 677
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1726528800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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