BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fner10d12f (624 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_47771| Best HMM Match : Annexin (HMM E-Value=0) 103 2e-22 SB_37033| Best HMM Match : Annexin (HMM E-Value=0) 101 6e-22 SB_35578| Best HMM Match : Annexin (HMM E-Value=1e-19) 87 8e-18 SB_26806| Best HMM Match : Annexin (HMM E-Value=0) 68 7e-12 SB_6006| Best HMM Match : Annexin (HMM E-Value=2.4e-25) 61 6e-10 SB_7681| Best HMM Match : Annexin (HMM E-Value=0) 51 9e-07 SB_29977| Best HMM Match : IL8 (HMM E-Value=1) 30 1.3 SB_24047| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 4.1 SB_37480| Best HMM Match : rve (HMM E-Value=4.7e-22) 27 9.4 >SB_47771| Best HMM Match : Annexin (HMM E-Value=0) Length = 529 Score = 103 bits (246), Expect = 2e-22 Identities = 50/104 (48%), Positives = 70/104 (67%) Frame = +1 Query: 295 TVVGVPNFNAVEDAAALRAAMKGFGTDEQAIIDILTTRSNMQRQAISQAFTREYGRDLIE 474 TVV NF+A + A L+ AMKGFG+DE+AII++L + + QR AI+Q+F ++Y +DL+ Sbjct: 295 TVVAAENFDAGDCAEKLKKAMKGFGSDEEAIIEVLQSCNLEQRLAIAQSFKQQYDKDLVN 354 Query: 475 DLKSELGGHFEDVIVALMTPPEEYLCQELHRCMEGMGTDEDTLV 606 +LKSEL G FED IVAL+ PPE+ LH M+ D +V Sbjct: 355 ELKSELSGKFEDAIVALLLPPEQLDANALHGAMQAKRVSADEIV 398 Score = 50.0 bits (114), Expect = 2e-06 Identities = 23/99 (23%), Positives = 48/99 (48%) Frame = +1 Query: 328 EDAAALRAAMKGFGTDEQAIIDILTTRSNMQRQAISQAFTREYGRDLIEDLKSELGGHFE 507 +D L+ A++ + + + + +S + + Y +DL+E L ++ Sbjct: 47 DDITKLQKAIEKSDKKTATEVIVCNASDEGKLKKLSDEYNKRYEKDLVEQLDGKVADDVH 106 Query: 508 DVIVALMTPPEEYLCQELHRCMEGMGTDEDTLVEILCTR 624 + + +L+ P + + L+ M+G+GT E L+E LCTR Sbjct: 107 EAVTSLLLPATQADVRSLYYAMKGLGTKEAQLIETLCTR 145 Score = 48.8 bits (111), Expect = 4e-06 Identities = 27/69 (39%), Positives = 39/69 (56%), Gaps = 1/69 (1%) Frame = +1 Query: 322 AVEDAAAL-RAAMKGFGTDEQAIIDILTTRSNMQRQAISQAFTREYGRDLIEDLKSELGG 498 A EDA L A K +GTDE ILT RS++Q +A+ QA+ D++E + EL G Sbjct: 403 AYEDAKNLYEAGEKKWGTDESVFTKILTCRSDLQLRALYQAYQHVAKCDILETIDDELTG 462 Query: 499 HFEDVIVAL 525 + D + A+ Sbjct: 463 DYHDAVKAI 471 Score = 31.9 bits (69), Expect = 0.43 Identities = 15/28 (53%), Positives = 18/28 (64%) Frame = +1 Query: 331 DAAALRAAMKGFGTDEQAIIDILTTRSN 414 D +L AMKG GT E +I+ L TRSN Sbjct: 120 DVRSLYYAMKGLGTKEAQLIETLCTRSN 147 >SB_37033| Best HMM Match : Annexin (HMM E-Value=0) Length = 287 Score = 101 bits (241), Expect = 6e-22 Identities = 45/86 (52%), Positives = 64/86 (74%) Frame = +1 Query: 367 GTDEQAIIDILTTRSNMQRQAISQAFTREYGRDLIEDLKSELGGHFEDVIVALMTPPEEY 546 G D +A++ +L +R+N QRQ IS + +GRDLI+DLKSE+GG+FED ++ALMTPP EY Sbjct: 101 GCDNKALMYLLCSRTNSQRQRISLEYKTMFGRDLIKDLKSEVGGYFEDTVIALMTPPAEY 160 Query: 547 LCQELHRCMEGMGTDEDTLVEILCTR 624 L + ++G+GTDE L+E+L TR Sbjct: 161 DATLLRKAIKGLGTDEAVLIEVLTTR 186 Score = 69.3 bits (162), Expect = 2e-12 Identities = 41/111 (36%), Positives = 61/111 (54%), Gaps = 1/111 (0%) Frame = +1 Query: 295 TVVGVPNFNAVEDAAALRAAMKGFGTDEQAIIDILTTRSNMQRQAISQAFTREYGRDLIE 474 TV+ + A DA LR A+KG GTDE +I++LTTR+N + AI A+ + RDL + Sbjct: 149 TVIALMTPPAEYDATLLRKAIKGLGTDEAVLIEVLTTRTNDEIIAIRNAYNTLFSRDLEK 208 Query: 475 DLKSELGGHFEDVIVALMTPPEEYLCQELHRCMEG-MGTDEDTLVEILCTR 624 D+ + G F+ +++ LC L++ EG GTDE IL +R Sbjct: 209 DIAGDTSGKFKKFLIS--------LCNALYKAGEGRWGTDESKFNSILASR 251 >SB_35578| Best HMM Match : Annexin (HMM E-Value=1e-19) Length = 146 Score = 87.4 bits (207), Expect = 8e-18 Identities = 42/85 (49%), Positives = 57/85 (67%) Frame = +1 Query: 370 TDEQAIIDILTTRSNMQRQAISQAFTREYGRDLIEDLKSELGGHFEDVIVALMTPPEEYL 549 T+E +I IL RSN QR I + + YG+DL+ DLKSEL G+ E+ ++A+M P Y Sbjct: 2 TNEAELIGILANRSNAQRVEIRKRYKTMYGKDLMNDLKSELSGNLEECLLAMMEPSVLYD 61 Query: 550 CQELHRCMEGMGTDEDTLVEILCTR 624 + L R M G GTDE+TL++ILCTR Sbjct: 62 AKCLRRGMRGAGTDEETLIDILCTR 86 Score = 40.7 bits (91), Expect = 0.001 Identities = 18/28 (64%), Positives = 21/28 (75%) Frame = +1 Query: 331 DAAALRAAMKGFGTDEQAIIDILTTRSN 414 DA LR M+G GTDE+ +IDIL TRSN Sbjct: 61 DAKCLRRGMRGAGTDEETLIDILCTRSN 88 >SB_26806| Best HMM Match : Annexin (HMM E-Value=0) Length = 829 Score = 67.7 bits (158), Expect = 7e-12 Identities = 31/66 (46%), Positives = 44/66 (66%) Frame = +1 Query: 331 DAAALRAAMKGFGTDEQAIIDILTTRSNMQRQAISQAFTREYGRDLIEDLKSELGGHFED 510 D +L AMKG GT E +I+IL TRSN + Q I Q + ++YGR L +D+KSE G FE+ Sbjct: 517 DVRSLYYAMKGLGTKEAQLIEILCTRSNAEIQEIKQEYNKKYGRSLTDDMKSETSGDFEN 576 Query: 511 VIVALM 528 ++ L+ Sbjct: 577 LLETLL 582 Score = 49.2 bits (112), Expect = 3e-06 Identities = 24/99 (24%), Positives = 48/99 (48%) Frame = +1 Query: 328 EDAAALRAAMKGFGTDEQAIIDILTTRSNMQRQAISQAFTREYGRDLIEDLKSELGGHFE 507 +D L+ A+ I + + + ++ + + Y +DL+E L ++ Sbjct: 444 DDITKLQKAIDKSDKKTTTAIIVSNASDEGKLKKLNDEYNKRYKKDLVEQLDGKVAIDVH 503 Query: 508 DVIVALMTPPEEYLCQELHRCMEGMGTDEDTLVEILCTR 624 + + +L+ P + + L+ M+G+GT E L+EILCTR Sbjct: 504 EAVTSLLLPATKADVRSLYYAMKGLGTKEAQLIEILCTR 542 Score = 39.1 bits (87), Expect = 0.003 Identities = 24/70 (34%), Positives = 39/70 (55%), Gaps = 2/70 (2%) Frame = +1 Query: 328 EDAAAL-RAAMKGFGTDEQAIIDILTTRSNMQRQAISQAF-TREYGRDLIEDLKSELGGH 501 +DAAAL A + +GTDE I IL+ RS+ QA+ + T G L + ++ E+ G Sbjct: 599 KDAAALVSAGVARWGTDEDQFITILSQRSSAHVQAVLAEYKTLSGGVSLADTVRDEMKGD 658 Query: 502 FEDVIVALMT 531 D ++ L++ Sbjct: 659 LRDALLVLIS 668 Score = 37.5 bits (83), Expect = 0.009 Identities = 15/35 (42%), Positives = 25/35 (71%) Frame = +1 Query: 427 AISQAFTREYGRDLIEDLKSELGGHFEDVIVALMT 531 +I + + ++YG L +D+K E G FED++VALM+ Sbjct: 90 SIKEEYQKKYGCSLEDDVKEETSGDFEDLLVALMS 124 Score = 35.9 bits (79), Expect = 0.027 Identities = 20/44 (45%), Positives = 28/44 (63%), Gaps = 1/44 (2%) Frame = +1 Query: 322 AVEDAAAL-RAAMKGFGTDEQAIIDILTTRSNMQRQAISQAFTR 450 A +DA AL A +K +GTDE I+I TTRS Q QA+ +++ Sbjct: 171 AHKDAEALIEAGIKKWGTDEDCFIEIFTTRSYPQLQAMFPEYSK 214 Score = 32.3 bits (70), Expect = 0.33 Identities = 21/57 (36%), Positives = 32/57 (56%), Gaps = 2/57 (3%) Frame = +1 Query: 328 EDAAAL-RAAMKGFGTDEQAIIDILTTRSNMQRQAISQAF-TREYGRDLIEDLKSEL 492 +DAAAL A + +GT+E I ILT RS+ QA+ + T G L + ++ E+ Sbjct: 772 QDAAALVSAGVARWGTEEDQFITILTQRSSAHVQAVLAEYRTLSGGVTLADTIRDEM 828 >SB_6006| Best HMM Match : Annexin (HMM E-Value=2.4e-25) Length = 803 Score = 61.3 bits (142), Expect = 6e-10 Identities = 36/107 (33%), Positives = 61/107 (57%), Gaps = 6/107 (5%) Frame = +1 Query: 322 AVEDAAALRAAMKGFGTD--EQAIIDILTT----RSNMQRQAISQAFTREYGRDLIEDLK 483 A EDA L A++G ++ + AIIDIL RS + R + + ++ +D++E L+ Sbjct: 346 AKEDADELHQAIQGMVSEGNKDAIIDILARNCDERSKLDR--LKDKYKDQHDQDILEALQ 403 Query: 484 SELGGHFEDVIVALMTPPEEYLCQELHRCMEGMGTDEDTLVEILCTR 624 LG +D + + + + + LH ++G+GTDED L+EI+CTR Sbjct: 404 PHLGDDVKDPVESFFSSSSAFDAKCLHDAIKGLGTDEDLLIEIICTR 450 Score = 47.2 bits (107), Expect = 1e-05 Identities = 31/88 (35%), Positives = 48/88 (54%), Gaps = 4/88 (4%) Frame = +1 Query: 373 DEQAIIDILTTRSNMQRQ-AISQAFTREYGRDLIEDLKSELGGHFEDVIVALMTPPEEYL 549 D++ I+ IL S + + + Y +DL E+LKS L E+++ AL + EE Sbjct: 21 DKERIMGILGRTSEKDKMNKLEDKYKDLYKKDLNEELKSVLD---EEIMKALESLAEEAA 77 Query: 550 CQE---LHRCMEGMGTDEDTLVEILCTR 624 ++ L+ +G GTDED L+ ILCTR Sbjct: 78 MKDAKTLYEATKGFGTDEDALISILCTR 105 Score = 44.0 bits (99), Expect = 1e-04 Identities = 26/55 (47%), Positives = 34/55 (61%), Gaps = 4/55 (7%) Frame = +1 Query: 322 AVEDAAALRAAMKGFGTDEQAIIDILTTRSNM-QRQAISQA---FTREYGRDLIE 474 A++DA L A KGFGTDE A+I IL TRSN QR +A ++ +DL+E Sbjct: 77 AMKDAKTLYEATKGFGTDEDALISILCTRSNKGQRDESKEADIHLAKKDAKDLME 131 Score = 35.1 bits (77), Expect = 0.047 Identities = 15/28 (53%), Positives = 19/28 (67%) Frame = +1 Query: 331 DAAALRAAMKGFGTDEQAIIDILTTRSN 414 DA L A+KG GTDE +I+I+ TR N Sbjct: 425 DAKCLHDAIKGLGTDEDLLIEIICTRKN 452 >SB_7681| Best HMM Match : Annexin (HMM E-Value=0) Length = 426 Score = 50.8 bits (116), Expect = 9e-07 Identities = 30/102 (29%), Positives = 51/102 (50%), Gaps = 1/102 (0%) Frame = +1 Query: 322 AVEDAAAL-RAAMKGFGTDEQAIIDILTTRSNMQRQAISQAFTREYGRDLIEDLKSELGG 498 A ++A L +A K +GTDE +L +RS Q QA + + RD++ + E+ G Sbjct: 14 ATKEAQDLYKAGEKKWGTDESRFNVVLASRSFPQLQATFNEYIKISQRDIMNSIDREMSG 73 Query: 499 HFEDVIVALMTPPEEYLCQELHRCMEGMGTDEDTLVEILCTR 624 + + P EY L + M+G GTD+ L+ ++ +R Sbjct: 74 DLKAGLQCARN-PAEYFADRLWKSMKGAGTDDSLLIRVVVSR 114 Score = 41.5 bits (93), Expect = 5e-04 Identities = 18/67 (26%), Positives = 40/67 (59%) Frame = +1 Query: 334 AAALRAAMKGFGTDEQAIIDILTTRSNMQRQAISQAFTREYGRDLIEDLKSELGGHFEDV 513 A L +MKG GTD+ +I ++ +RS + I +F ++Y + + + ++ + G ++ + Sbjct: 90 ADRLWKSMKGAGTDDSLLIRVVVSRSEVDLVEIKASFLQKYHKTVYKMIEGDCSGDYKKL 149 Query: 514 IVALMTP 534 ++AL +P Sbjct: 150 LMALPSP 156 >SB_29977| Best HMM Match : IL8 (HMM E-Value=1) Length = 521 Score = 30.3 bits (65), Expect = 1.3 Identities = 19/59 (32%), Positives = 30/59 (50%) Frame = +1 Query: 130 EILYTALIKRNCFFYNSHTVSPS*KFGSAENRRSPFSIFDTNPRILTEMAYQRAPTVVG 306 +ILY KR ++S + + S FGSA N + + I T+ L+ +A R P +G Sbjct: 176 QILYIVHSKRAALLFSSQSPAVSSWFGSAYNTLAKYFILFTSREQLSCLAVSRQPFRLG 234 >SB_24047| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 2905 Score = 28.7 bits (61), Expect = 4.1 Identities = 12/24 (50%), Positives = 14/24 (58%) Frame = -3 Query: 589 PCPYPPYNGAALGTDTPQGESSEP 518 PCP P+ GT TPQG + EP Sbjct: 1712 PCPSQPHITICPGTGTPQGITIEP 1735 >SB_37480| Best HMM Match : rve (HMM E-Value=4.7e-22) Length = 900 Score = 27.5 bits (58), Expect = 9.4 Identities = 13/37 (35%), Positives = 21/37 (56%) Frame = +1 Query: 397 LTTRSNMQRQAISQAFTREYGRDLIEDLKSELGGHFE 507 L R M+R+ + Q + REY L+E + + G HF+ Sbjct: 340 LHARLMMKREQVWQRWRREYIHSLMESHRVKRGEHFD 376 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 20,340,855 Number of Sequences: 59808 Number of extensions: 434461 Number of successful extensions: 1088 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 995 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1087 length of database: 16,821,457 effective HSP length: 79 effective length of database: 12,096,625 effective search space used: 1548368000 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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