BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fner10d04f (555 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC576.07 |ret3||coatomer zeta subunit |Schizosaccharomyces pom... 101 7e-23 SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4 |Schizosac... 36 0.005 SPCC16C4.02c |||DUF1941 family protein|Schizosaccharomyces pombe... 25 5.7 SPBC530.06c |||translation initiation factor eIF3 alpha subunit ... 25 9.9 SPBC1703.14c |top1||DNA topoisomerase I|Schizosaccharomyces pomb... 25 9.9 >SPCC576.07 |ret3||coatomer zeta subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 190 Score = 101 bits (242), Expect = 7e-23 Identities = 53/146 (36%), Positives = 80/146 (54%), Gaps = 8/146 (5%) Frame = +1 Query: 112 TLYIVKGMCILDYEGNRILAKYY--------DKDVLPTTKEQKAFEKNLFNKTHRAXAEI 267 TLY V ILD G RI KYY + V + KE+K FEK LF KT + +I Sbjct: 4 TLYAVNAFLILDSSGKRIFTKYYAPPHLKEGEGGVFNSVKEEKTFEKGLFEKTWKTQNDI 63 Query: 268 IMLDGLTCVYKSXVDLFFYVMGSSHEXELILQSXLNALYXSVSLLLRRNMERRVLMENLD 447 + DG V + +D+ FY++G E E++L L ++ ++ LL + ++R L+EN D Sbjct: 64 LTYDGKLVVMLTVMDVIFYIVGGMEENEVMLYECLRSIRDALELLFKYVPDKRTLLENYD 123 Query: 448 AVMLAFDEICDGGVILDADPTSIVSR 525 +++ DE D GVIL+ +P I +R Sbjct: 124 QLVIVVDETIDDGVILETEPALIAAR 149 >SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4 |Schizosaccharomyces pombe|chr 1|||Manual Length = 446 Score = 35.5 bits (78), Expect = 0.005 Identities = 25/138 (18%), Positives = 59/138 (42%) Frame = +1 Query: 121 IVKGMCILDYEGNRILAKYYDKDVLPTTKEQKAFEKNLFNKTHRAXAEIIMLDGLTCVYK 300 ++ G+ I + +G+ ++ K + D+ + E F + T I+ + T +Y Sbjct: 1 MISGLFIFNLKGDTLICKTFRHDLKKSVTE--IFRVAILTNTDYRHP-IVSIGSSTYIYT 57 Query: 301 SXVDLFFYVMGSSHEXELILQSXLNALYXSVSLLLRRNMERRVLMENLDAVMLAFDEICD 480 DL+ + + +I+ L +L ++ + E V +N+ + DE+ D Sbjct: 58 KHEDLYVVAITKGNPNVMIVLEFLESLIQDLTHYFGKLNENTV-KDNVSFIFELLDEMID 116 Query: 481 GGVILDADPTSIVSRAAL 534 G+I +P ++ ++ Sbjct: 117 YGIIQTTEPDALARSVSI 134 >SPCC16C4.02c |||DUF1941 family protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 548 Score = 25.4 bits (53), Expect = 5.7 Identities = 9/21 (42%), Positives = 13/21 (61%) Frame = +2 Query: 413 TWSGGSSWRTWMLLCLPSMRS 475 +WS G + W+ + LPSM S Sbjct: 437 SWSNGIDYAKWISVALPSMLS 457 >SPBC530.06c |||translation initiation factor eIF3 alpha subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 1173 Score = 24.6 bits (51), Expect = 9.9 Identities = 7/15 (46%), Positives = 13/15 (86%) Frame = +1 Query: 130 GMCILDYEGNRILAK 174 G C++DY G+R++A+ Sbjct: 457 GTCVVDYAGHRVVAQ 471 >SPBC1703.14c |top1||DNA topoisomerase I|Schizosaccharomyces pombe|chr 2|||Manual Length = 814 Score = 24.6 bits (51), Expect = 9.9 Identities = 10/35 (28%), Positives = 21/35 (60%) Frame = +1 Query: 181 DKDVLPTTKEQKAFEKNLFNKTHRAXAEIIMLDGL 285 D+D +P K + + +KN+ N + + A+++ GL Sbjct: 55 DEDDIPIRKRRASSKKNMSNSSSKKRAKVMGNGGL 89 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,089,423 Number of Sequences: 5004 Number of extensions: 38570 Number of successful extensions: 89 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 86 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 88 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 231978230 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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