Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner10c03f
(607 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g32720.1 68415.m04004 cytochrome b5, putative similar to Cyto... 52 2e-07
At1g77760.1 68414.m09053 nitrate reductase 1 (NR1) identical to ... 50 9e-07
At1g37130.1 68414.m04639 nitrate reductase 2 (NR2) identical to ... 50 1e-06
At5g48810.1 68418.m06039 cytochrome b5 identical to cytochrome b... 49 3e-06
At5g53560.1 68418.m06655 cytochrome b5 isoform 1 identical to SP... 42 2e-04
At1g26340.1 68414.m03212 cytochrome b5, putative similar to cyto... 42 2e-04
At5g09680.1 68418.m01120 cytochrome b5 domain-containing protein... 41 7e-04
At2g46210.1 68415.m05746 delta-8 sphingolipid desaturase, putati... 38 0.005
At2g46650.1 68415.m05820 cytochrome b5, putative similar to cyto... 37 0.012
At3g61580.1 68416.m06897 delta-8 sphingolipid desaturase (SLD1) ... 36 0.016
At1g60660.1 68414.m06829 cytochrome b5 domain-containing protein... 33 0.19
At4g22970.1 68417.m03315 peptidase C50 family protein contains P... 29 1.8
At1g57570.1 68414.m06533 jacalin lectin family protein similar t... 29 1.8
At5g22220.2 68418.m02588 E2F transcription factor-1 (E2F1) ident... 29 3.2
At5g22220.1 68418.m02587 E2F transcription factor-1 (E2F1) ident... 29 3.2
At5g10150.1 68418.m01175 expressed protein 28 4.2
At1g78730.1 68414.m09176 F-box family protein contains F-box dom... 28 4.2
At1g47920.1 68414.m05337 syntaxin-related family protein contain... 27 9.6
>At2g32720.1 68415.m04004 cytochrome b5, putative similar to
Cytochrome B5 SP:P49098 from [Nicotiana tabacum]
Length = 134
Score = 52.4 bits (120), Expect = 2e-07
Identities = 27/61 (44%), Positives = 35/61 (57%), Gaps = 1/61 (1%)
Frame = +1
Query: 205 AEGLWRVHNG-IYNFNDFLEKHPGGAEWLELSKGTDITEAFESHHLNSSVNKVLEKYYVR 381
A W V NG +YN FLE HPGG + L S G D T+ FE + S +++E+YYV
Sbjct: 19 AHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFEDVGHSESAREMMEQYYVG 78
Query: 382 E 384
E
Sbjct: 79 E 79
>At1g77760.1 68414.m09053 nitrate reductase 1 (NR1) identical to
SP|P11832 Nitrate reductase 1 (formerly EC 1.6.6.1)
(NR1){Arabidopsis thaliana}
Length = 917
Score = 50.4 bits (115), Expect = 9e-07
Identities = 26/64 (40%), Positives = 37/64 (57%), Gaps = 1/64 (1%)
Frame = +1
Query: 205 AEGLWR-VHNGIYNFNDFLEKHPGGAEWLELSKGTDITEAFESHHLNSSVNKVLEKYYVR 381
A+ W VH IY+ FL+ HPGG + + ++ GTD TE FE+ H + K+LE Y +
Sbjct: 559 ADSAWIIVHGHIYDCTRFLKDHPGGTDSILINAGTDCTEEFEAIH-SDKAKKLLEDYRIG 617
Query: 382 EAKT 393
E T
Sbjct: 618 ELIT 621
>At1g37130.1 68414.m04639 nitrate reductase 2 (NR2) identical to
SP|P11035 Nitrate reductase 2 (formerly EC 1.6.6.1)
(NR2) {Arabidopsis thaliana}
Length = 917
Score = 50.0 bits (114), Expect = 1e-06
Identities = 26/66 (39%), Positives = 38/66 (57%), Gaps = 1/66 (1%)
Frame = +1
Query: 199 DGAEGLWR-VHNGIYNFNDFLEKHPGGAEWLELSKGTDITEAFESHHLNSSVNKVLEKYY 375
+ A+ W VH IY+ FL HPGG++ + ++ GTD TE FE+ H + K+LE Y
Sbjct: 554 NSADSCWIIVHGHIYDCTRFLMDHPGGSDSILINAGTDCTEEFEAIH-SDKAKKMLEDYR 612
Query: 376 VREAKT 393
+ E T
Sbjct: 613 IGELIT 618
>At5g48810.1 68418.m06039 cytochrome b5 identical to cytochrome b5
[Arabidopsis thaliana] GI:4240122; strong similarity to
Cytochrome B5 SP:P49098 from [Nicotiana tabacum]
Length = 140
Score = 48.8 bits (111), Expect = 3e-06
Identities = 25/69 (36%), Positives = 38/69 (55%), Gaps = 1/69 (1%)
Frame = +1
Query: 190 SMDDGAEGLWRVHNG-IYNFNDFLEKHPGGAEWLELSKGTDITEAFESHHLNSSVNKVLE 366
S A+ W V +G +Y+ FL+ HPGG E + S G D T+ FE +S+ +L+
Sbjct: 14 SQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFEDVGHSSTAKAMLD 73
Query: 367 KYYVREAKT 393
+YYV + T
Sbjct: 74 EYYVGDIDT 82
>At5g53560.1 68418.m06655 cytochrome b5 isoform 1 identical to
SP|Q42342 Cytochrome b5 isoform 1 [Arabidopsis thaliana]
Length = 134
Score = 42.3 bits (95), Expect = 2e-04
Identities = 19/57 (33%), Positives = 32/57 (56%), Gaps = 1/57 (1%)
Frame = +1
Query: 217 WRVHNG-IYNFNDFLEKHPGGAEWLELSKGTDITEAFESHHLNSSVNKVLEKYYVRE 384
W + +G +Y+ F++ HPGG E L S G D T FE + + +++KY++ E
Sbjct: 23 WLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFEDVGHSDTARDMMDKYFIGE 79
>At1g26340.1 68414.m03212 cytochrome b5, putative similar to
cytochrome b5 GB:BAA74839 GI:4240120 from [Arabidopsis
thaliana]
Length = 135
Score = 42.3 bits (95), Expect = 2e-04
Identities = 18/57 (31%), Positives = 34/57 (59%), Gaps = 1/57 (1%)
Frame = +1
Query: 217 WRVHNG-IYNFNDFLEKHPGGAEWLELSKGTDITEAFESHHLNSSVNKVLEKYYVRE 384
W V +G +Y+ + ++++HPGG + L G D T+ FE + +++EKY++ E
Sbjct: 23 WVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHSKDARELMEKYFIGE 79
>At5g09680.1 68418.m01120 cytochrome b5 domain-containing protein
flavohemoprotein b5+b5R, Homo sapiens, EMBL:AF169803
Length = 211
Score = 40.7 bits (91), Expect = 7e-04
Identities = 20/58 (34%), Positives = 32/58 (55%), Gaps = 1/58 (1%)
Frame = +1
Query: 208 EGLWRVHNG-IYNFNDFLEKHPGGAEWLELSKGTDITEAFESHHLNSSVNKVLEKYYV 378
+ +W V G +YN + ++ HPGG + L + G D T F +H +V+ +LEK V
Sbjct: 141 DSMWTVLKGRVYNISPYMNFHPGGVDMLMKAVGRDGTLLFNKYHAWVNVDILLEKCLV 198
>At2g46210.1 68415.m05746 delta-8 sphingolipid desaturase, putative
similar to delta-8 sphingolipid desaturase GI:3819708
from [Brassica napus]
Length = 449
Score = 37.9 bits (84), Expect = 0.005
Identities = 18/61 (29%), Positives = 36/61 (59%), Gaps = 4/61 (6%)
Frame = +1
Query: 214 LW-RVHNGIYNFNDFLEKHPGGAEWLELSKGTDITEAFESHHLNSS---VNKVLEKYYVR 381
LW + +Y+ +D+++ HPGG + G D+T+AF ++H ++ + K+ Y+VR
Sbjct: 24 LWISIQGKVYDVSDWVKSHPGGEAAILNLAGQDVTDAFIAYHPGTAWHHLEKLHNGYHVR 83
Query: 382 E 384
+
Sbjct: 84 D 84
>At2g46650.1 68415.m05820 cytochrome b5, putative similar to
cytochome b5 GI:2695711 from [Olea europaea]
Length = 132
Score = 36.7 bits (81), Expect = 0.012
Identities = 14/52 (26%), Positives = 29/52 (55%)
Frame = +1
Query: 223 VHNGIYNFNDFLEKHPGGAEWLELSKGTDITEAFESHHLNSSVNKVLEKYYV 378
+H +Y+ + F+++HPGG L G D + FE + + ++++KY +
Sbjct: 23 IHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSKDAKELMKKYCI 74
>At3g61580.1 68416.m06897 delta-8 sphingolipid desaturase (SLD1)
identical to delta-8 sphingolipid desaturase GI:3819710
from [Arabidopsis thaliana]; contains Pfam profile
PF00487: Fatty acid desaturase; contains Pfam profile
PF00173: Heme/Steroid binding domain
Length = 449
Score = 36.3 bits (80), Expect = 0.016
Identities = 18/61 (29%), Positives = 35/61 (57%), Gaps = 4/61 (6%)
Frame = +1
Query: 214 LWRVHNG-IYNFNDFLEKHPGGAEWLELSKGTDITEAFESHHLNSS---VNKVLEKYYVR 381
LW G +YN +D+++ HPGG + G D+T+AF + H ++ ++ + Y++R
Sbjct: 24 LWIAIQGKVYNVSDWIKTHPGGDTVILNLVGQDVTDAFIAFHPGTAWHHLDHLFTGYHIR 83
Query: 382 E 384
+
Sbjct: 84 D 84
>At1g60660.1 68414.m06829 cytochrome b5 domain-containing protein
contains InterPro accession IPR001199: Cytochrome b5
Length = 121
Score = 32.7 bits (71), Expect = 0.19
Identities = 15/54 (27%), Positives = 29/54 (53%)
Frame = +1
Query: 223 VHNGIYNFNDFLEKHPGGAEWLELSKGTDITEAFESHHLNSSVNKVLEKYYVRE 384
+ + +Y+ ++E+HPGG L+ G D T+ F + V ++E +Y+ E
Sbjct: 67 IKDKVYDITSYVEEHPGGDAILD-HAGDDSTDGFFGPQHATRVFDMIEDFYIGE 119
>At4g22970.1 68417.m03315 peptidase C50 family protein contains Pfam
PF03568: Peptidase family C50
Length = 1773
Score = 29.5 bits (63), Expect = 1.8
Identities = 16/38 (42%), Positives = 22/38 (57%)
Frame = +1
Query: 316 EAFESHHLNSSVNKVLEKYYVREAKTPRNSPFTFEDDG 429
E ES LN+ V+++L Y+ + NS FTFE DG
Sbjct: 1160 EVTESGSLNNLVSRLLASLYLLSSS---NSEFTFESDG 1194
>At1g57570.1 68414.m06533 jacalin lectin family protein similar to
myrosinase-binding protein homolog [Arabidopsis
thaliana] GI:2997767; contains Pfam profile PF01419
jacalin-like lectin domain
Length = 614
Score = 29.5 bits (63), Expect = 1.8
Identities = 40/152 (26%), Positives = 67/152 (44%), Gaps = 21/152 (13%)
Frame = +1
Query: 184 GKSMDDGA--EGLWRVH-----NGI-YNFNDFLEK---HPGGAEWLELSKGTDITEAFES 330
G DDG +G+ ++H GI Y D++ K H G+ + G+ T+ FE
Sbjct: 183 GNQWDDGTNNDGVTKIHVRGGVEGIQYIKFDYVRKSGQHINGS--IHGLSGSGFTQTFEI 240
Query: 331 HHLNSSVNKVLEKYYVREAKTPRNSPF-----TFEDDGFYRTLKRAVVEELKKVPKHIPS 495
HLN+ +E YY E+ + F T E G+ + K ++V++ KK+
Sbjct: 241 DHLNNEHLVCVEGYYDDESGVIQALQFKTNIKTSELLGYKKGKKFSLVDKRKKI-VGFHG 299
Query: 496 YADMIID--GLFATLLISSAVSCWAXD---YW 576
YAD ++ G + T + + C+ YW
Sbjct: 300 YADKNLNSLGAYFTTVSPTKSECYGSSKGIYW 331
>At5g22220.2 68418.m02588 E2F transcription factor-1 (E2F1)
identical to E2F transcription factor-1 E2F1
[Arabidopsis thaliana] gi|10443849|gb|AAG17608
Length = 469
Score = 28.7 bits (61), Expect = 3.2
Identities = 24/102 (23%), Positives = 39/102 (38%)
Frame = +1
Query: 124 QLKYPSLRDEGLRDPVQWLIGKSMDDGAEGLWRVHNGIYNFNDFLEKHPGGAEWLELSKG 303
Q Y + D + ++ +D G + +R G + D G +W ++
Sbjct: 375 QRVYSDIESHDFVDGIMKIVPPDLDMGVDYWFRSEVGEVSITDMWPDE-SGPDWNQM--- 430
Query: 304 TDITEAFESHHLNSSVNKVLEKYYVREAKTPRNSPFTFEDDG 429
IT F+ H S NK+LE+ + TP S T G
Sbjct: 431 --IT--FDQDHAGPSDNKILEQPQTPSSPTPEESTATRSPTG 468
>At5g22220.1 68418.m02587 E2F transcription factor-1 (E2F1)
identical to E2F transcription factor-1 E2F1
[Arabidopsis thaliana] gi|10443849|gb|AAG17608
Length = 469
Score = 28.7 bits (61), Expect = 3.2
Identities = 24/102 (23%), Positives = 39/102 (38%)
Frame = +1
Query: 124 QLKYPSLRDEGLRDPVQWLIGKSMDDGAEGLWRVHNGIYNFNDFLEKHPGGAEWLELSKG 303
Q Y + D + ++ +D G + +R G + D G +W ++
Sbjct: 375 QRVYSDIESHDFVDGIMKIVPPDLDMGVDYWFRSEVGEVSITDMWPDE-SGPDWNQM--- 430
Query: 304 TDITEAFESHHLNSSVNKVLEKYYVREAKTPRNSPFTFEDDG 429
IT F+ H S NK+LE+ + TP S T G
Sbjct: 431 --IT--FDQDHAGPSDNKILEQPQTPSSPTPEESTATRSPTG 468
>At5g10150.1 68418.m01175 expressed protein
Length = 414
Score = 28.3 bits (60), Expect = 4.2
Identities = 14/50 (28%), Positives = 26/50 (52%), Gaps = 4/50 (8%)
Frame = +1
Query: 298 KGTDITEAFESHHLN----SSVNKVLEKYYVREAKTPRNSPFTFEDDGFY 435
KG++IT+ F+ H+N S+ + + +R P+N +F+D Y
Sbjct: 129 KGSEITDKFQEVHVNRPLSGSIQEAPKSRLLRSKLKPQNRTASFDDAELY 178
>At1g78730.1 68414.m09176 F-box family protein contains F-box domain
Pfam:PF00646
Length = 420
Score = 28.3 bits (60), Expect = 4.2
Identities = 12/28 (42%), Positives = 21/28 (75%)
Frame = +2
Query: 53 LKMLNILK*LIEEQQKRKLMSVFLNSNT 136
L+ ++ILK ++E++ + KLMS FL +T
Sbjct: 354 LEFVDILKPMVEKETELKLMSYFLEKST 381
>At1g47920.1 68414.m05337 syntaxin-related family protein contains a
novel domain similar to F-box that is shared among other
proteins in Arabidopsis;
Length = 379
Score = 27.1 bits (57), Expect = 9.6
Identities = 14/36 (38%), Positives = 21/36 (58%), Gaps = 1/36 (2%)
Frame = -2
Query: 108 SFLFCCSSMSYFN-IFNIFRRHINKIRAFTSICLLI 4
+F FCC SM FN + N+F NK + + + LL+
Sbjct: 224 AFYFCCKSMPVFNKLLNLFIES-NKDKGWQGMPLLL 258
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,357,608
Number of Sequences: 28952
Number of extensions: 251340
Number of successful extensions: 767
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 747
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 767
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1206913392
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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