BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fner10b21r (767 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_14952| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 2.4 SB_3393| Best HMM Match : RNA_pol_Rpb1_R (HMM E-Value=0.012) 29 4.1 SB_50520| Best HMM Match : zf-DNL (HMM E-Value=3.4) 28 7.2 SB_11395| Best HMM Match : 7tm_1 (HMM E-Value=6.3e-06) 28 9.5 >SB_14952| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 451 Score = 29.9 bits (64), Expect = 2.4 Identities = 23/76 (30%), Positives = 35/76 (46%), Gaps = 6/76 (7%) Frame = +3 Query: 99 YTKIITLHIVTRISDITLISYSFFFKI------LPFIRKNTSNICTANTTMKHQKYLIAS 260 Y + H TR L++YSF K F RK ++ T + TMK+Q Y + + Sbjct: 185 YQYSLVTHSFTRKYQYPLVTYSFTRKYQYSLVTYSFTRKYQYSLVTHSFTMKYQ-YSLVT 243 Query: 261 LSTKKKSLNNFLIYSF 308 S +K + + YSF Sbjct: 244 YSFTRKYQYSLVTYSF 259 Score = 29.1 bits (62), Expect = 4.1 Identities = 25/89 (28%), Positives = 41/89 (46%), Gaps = 6/89 (6%) Frame = +3 Query: 99 YTKIITLHIVTRISDITLISYSFFFKI------LPFIRKNTSNICTANTTMKHQKYLIAS 260 Y + H TR +L++YSF K F RK ++ T + T K+Q Y + Sbjct: 341 YQYSLVTHSFTRKYQYSLVTYSFTRKYQYSLVTYSFTRKYQYSLVTHSFTRKYQ-YSLVK 399 Query: 261 LSTKKKSLNNFLIYSFIYIQKKRYYIFRF 347 S KK + + YSF +K +Y + ++ Sbjct: 400 YSFTKKYQYSLVKYSF--TRKYQYSLVKY 426 Score = 28.3 bits (60), Expect = 7.2 Identities = 22/76 (28%), Positives = 35/76 (46%), Gaps = 6/76 (7%) Frame = +3 Query: 99 YTKIITLHIVTRISDITLISYSFFFKI------LPFIRKNTSNICTANTTMKHQKYLIAS 260 Y + H TR +L++YSF K F RK ++ T + T K+Q Y + + Sbjct: 55 YQYSLVTHSFTRKYQYSLVTYSFTRKYQYSLVTYSFTRKYQYSLVTYSFTRKYQ-YSLVT 113 Query: 261 LSTKKKSLNNFLIYSF 308 S +K + + YSF Sbjct: 114 YSFTRKYQYSLVTYSF 129 Score = 28.3 bits (60), Expect = 7.2 Identities = 25/97 (25%), Positives = 44/97 (45%), Gaps = 6/97 (6%) Frame = +3 Query: 36 HGM*HTYLTPGYLFIGFQKFIYTKIITLHIVTRISDITLISYSFFFKI------LPFIRK 197 H Y P + +K+ Y+ + + TR +L+++SF K F RK Sbjct: 192 HSFTRKYQYPLVTYSFTRKYQYS--LVTYSFTRKYQYSLVTHSFTMKYQYSLVTYSFTRK 249 Query: 198 NTSNICTANTTMKHQKYLIASLSTKKKSLNNFLIYSF 308 ++ T + T K+Q YL+ + S +K + + YSF Sbjct: 250 YQYSLVTYSFTRKYQ-YLLVTYSFTRKYQYSLVTYSF 285 >SB_3393| Best HMM Match : RNA_pol_Rpb1_R (HMM E-Value=0.012) Length = 626 Score = 29.1 bits (62), Expect = 4.1 Identities = 16/44 (36%), Positives = 26/44 (59%), Gaps = 3/44 (6%) Frame = -2 Query: 256 AIKYFWCFIVVFAVHILDVF---FLINGNILKKKLYDINVISLI 134 A YF FI+V + +L++F + N N LKKK +IN + ++ Sbjct: 66 AYAYFVVFIIVGSFFVLNLFVGVIIDNFNTLKKKYEEINSMGML 109 >SB_50520| Best HMM Match : zf-DNL (HMM E-Value=3.4) Length = 277 Score = 28.3 bits (60), Expect = 7.2 Identities = 18/68 (26%), Positives = 39/68 (57%), Gaps = 3/68 (4%) Frame = +3 Query: 237 HQKYLIASLSTKKKSLNN--FLIYSFIYIQKKRYYIFRFMV*NIILSNTYNILNIFFSC- 407 H + + +S ++ L+ F I + I++Q + + R ++ II N Y+ +++FF+C Sbjct: 164 HARGEVKEISRREMLLHGDIFGILTGIFMQDGPFLVLRLVL--IIKYNLYSEMHLFFTCK 221 Query: 408 NVIAIHVI 431 N IA+ ++ Sbjct: 222 NAIALSLL 229 >SB_11395| Best HMM Match : 7tm_1 (HMM E-Value=6.3e-06) Length = 672 Score = 27.9 bits (59), Expect = 9.5 Identities = 13/43 (30%), Positives = 25/43 (58%), Gaps = 2/43 (4%) Frame = -2 Query: 265 DRDAIKYFWCFIVVFAVHIL--DVFFLINGNILKKKLYDINVI 143 ++D I+ F+ I VF + ++F+I N+LK Y +N++ Sbjct: 547 EKDLIRMFYIIIFVFVLFYFPYQIYFMIENNVLKDWYY-VNLL 588 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 17,073,711 Number of Sequences: 59808 Number of extensions: 272759 Number of successful extensions: 557 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 499 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 552 length of database: 16,821,457 effective HSP length: 81 effective length of database: 11,977,009 effective search space used: 2083999566 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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