SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fner10b06f
         (640 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC22F8.06 |pam1||20S proteasome component beta 6|Schizosacchar...   162   3e-41
SPCC63.12c |||20S proteasome component beta 3|Schizosaccharomyce...    83   2e-17
SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyc...    51   1e-07
SPAC23D3.07 |pup1||20S proteasome component beta 2|Schizosacchar...    45   8e-06
SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Sch...    30   0.25 
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce...    29   0.75 
SPBC646.03 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces...    28   1.3  
SPAC6C3.06c |||P-type ATPase, calcium transporting|Schizosacchar...    27   1.7  
SPAC56E4.06c |ggt2||gamma-glutamyltranspeptidase Ggt2|Schizosacc...    26   4.0  
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po...    26   4.0  
SPMIT.06 |||mitochondrial DNA binding endonuclease|Schizosacchar...    25   7.0  
SPBC365.02c |cox10||protoheme IX farnesyltransferase|Schizosacch...    25   7.0  
SPAC29B12.01 |ino80|SPAC3G6.12|SNF2 family helicase Ino80|Schizo...    25   7.0  
SPBC1289.01c |chr4|cfh3, SPBC1539.11c|chitin synthase regulatory...    25   9.2  
SPCC1183.08c |rpl101|rpl1-1, rpl10a-1|60S ribosomal protein L10a...    25   9.2  
SPBC577.09 |||ERCC-8 homolog |Schizosaccharomyces pombe|chr 2|||...    25   9.2  
SPCC23B6.04c |||sec14 cytosolic factor family|Schizosaccharomyce...    25   9.2  
SPAC17G6.05c |||Rhophilin-2 homolog|Schizosaccharomyces pombe|ch...    25   9.2  
SPBC26H8.12 |||cytochrome c heme lyase|Schizosaccharomyces pombe...    25   9.2  

>SPAC22F8.06 |pam1||20S proteasome component beta
           6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 225

 Score =  162 bits (394), Expect = 3e-41
 Identities = 73/159 (45%), Positives = 105/159 (66%)
 Frame = +2

Query: 131 QHRFEPYADNGGSIVAIAGDDYAVIGADTRLSTGFSIYTRDQKKLFKLSESTVLGATGCW 310
           Q +F+PY  NGG+ VAIAGD +A++  DTR   G++I TR Q ++ ++ +  V+GA+G  
Sbjct: 3   QSQFDPYVQNGGTTVAIAGDGFAILAGDTRSVNGYNINTRFQPRVHEVGDDLVIGASGFE 62

Query: 311 CDTLTLTRLLQARMQMYEHEHNKSMTTPAVAQMLSTMLYYKRFFPYYVSNVLAGLDSDGK 490
            D L L + +Q R+ +Y   H + M+  + A M+ T+LY KRFFPYYV   +AG+D +GK
Sbjct: 63  ADALALVKRIQQRIDLYHDNHERKMSAQSCACMVRTLLYGKRFFPYYVYTTVAGIDKEGK 122

Query: 491 GCVYSYDPIGHCARHNFRAGGSAAAQLQPLLDNQIGLKN 607
           G +YS+DP+G   R   RAGGSAA  + P LDNQ+ L N
Sbjct: 123 GEIYSFDPVGSYEREWCRAGGSAANFITPFLDNQVNLHN 161


>SPCC63.12c |||20S proteasome component beta 3|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 204

 Score = 83.4 bits (197), Expect = 2e-17
 Identities = 47/146 (32%), Positives = 75/146 (51%), Gaps = 2/146 (1%)
 Frame = +2

Query: 158 NGGSIVAIAGDDYAVIGADTRLSTGFSIYTRDQKKLFKLSESTVLGATGCWCDTLTLTRL 337
           NGGS VA+AG +   I +D RL       T +  K+F + + T LG TG   D  TL  L
Sbjct: 7   NGGSCVAMAGKNCVAIASDLRLGVQSISLTNNFPKVFAMGDKTYLGLTGLATDVQTLYEL 66

Query: 338 LQARMQMYEHEHNKSMTTPAVAQMLSTMLYYKRFFPYYVSNVLAGLDSDGKGCVYSYDPI 517
            + ++ +Y+    + +     A ++S+ LY KRF PY+   V+AG+ +D    +  +D I
Sbjct: 67  FRYKVNLYKFREERQIQPKTFANLVSSTLYEKRFGPYFSFPVVAGVSNDNTPFICGFDSI 126

Query: 518 GHCA--RHNFRAGGSAAAQLQPLLDN 589
           G C     +F   G+A  QL  + ++
Sbjct: 127 G-CIDFAEDFIVSGTATEQLYGMCES 151


>SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 226

 Score = 51.2 bits (117), Expect = 1e-07
 Identities = 38/142 (26%), Positives = 61/142 (42%)
 Frame = +2

Query: 161 GGSIVAIAGDDYAVIGADTRLSTGFSIYTRDQKKLFKLSESTVLGATGCWCDTLTLTRLL 340
           G +I A+   D  ++ AD+R + G  I  R   KL +L+++     +G   DT T+  LL
Sbjct: 24  GTTITALRYKDGVILAADSRTTMGAYIANRVTDKLTQLTDNIWCCRSGSAADTQTVADLL 83

Query: 341 QARMQMYEHEHNKSMTTPAVAQMLSTMLYYKRFFPYYVSNVLAGLDSDGKGCVYSYDPIG 520
           +  + MY  +     +    A + S M Y  +        ++AG D    G VYS    G
Sbjct: 84  KYYLSMYRIQFGHDPSVHTAATLASEMCYQNKNM-LSAGLIVAGYDEKTGGDVYSIPLGG 142

Query: 521 HCARHNFRAGGSAAAQLQPLLD 586
              +     GGS +A +    D
Sbjct: 143 SLHKQPLAIGGSGSAFIYGFCD 164


>SPAC23D3.07 |pup1||20S proteasome component beta
           2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 267

 Score = 45.2 bits (102), Expect = 8e-06
 Identities = 29/110 (26%), Positives = 52/110 (47%), Gaps = 1/110 (0%)
 Frame = +2

Query: 161 GGSIVAIAGDDYAVIGADTRLSTGFSIYTRDQKKLFKLSESTVLGATGCWCDTLTLTRLL 340
           G +IV +   D  V+GADTR + G  I  ++ KKL  +S +      G   DT  +T ++
Sbjct: 35  GTTIVGVIAKDCIVLGADTRATAGPIIADKNCKKLHLISPNIWCAGAGTAADTEFVTSMI 94

Query: 341 QARMQMYE-HEHNKSMTTPAVAQMLSTMLYYKRFFPYYVSNVLAGLDSDG 487
            + ++++  + + K     A+  +   +  Y+     Y+  VL G D  G
Sbjct: 95  SSNIELHSLYTNRKPRVVTALTMLKQHLFRYQGHIGAYL--VLGGYDCKG 142


>SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha
           3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 248

 Score = 30.3 bits (65), Expect = 0.25
 Identities = 30/162 (18%), Positives = 64/162 (39%), Gaps = 4/162 (2%)
 Frame = +2

Query: 155 DNGGSIVAIAGDDYAVIGADTRLSTGFSIYTRDQKKLFKLSESTVLGATGCWCDTLTLTR 334
           ++ G  + I   D  V+ A+ ++++         +KL+ + ++ +    G   D   L  
Sbjct: 29  NHAGVALGIVAKDGIVLAAEKKVTSKLLEQEESAEKLYHIGDNMLCAVAGLTADANILIN 88

Query: 335 LLQARMQMYEHEHNKSMTTPAVAQMLSTM----LYYKRFFPYYVSNVLAGLDSDGKGCVY 502
             +   Q Y    N+ M    + + +  +      Y    P+ VS + AG D      ++
Sbjct: 89  YARRVGQQYLQTFNEEMPCEQLVRRVCDLKQGYTQYGGLRPFGVSFLYAGWDHIRGYQLF 148

Query: 503 SYDPIGHCARHNFRAGGSAAAQLQPLLDNQIGLKNMQNVTEA 628
             +P G+       + G  +  +Q L+  +   K+  N+ EA
Sbjct: 149 QSNPSGNYGSWQANSIGGNSTSVQSLMRQE--YKDDINLDEA 188


>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 983

 Score = 28.7 bits (61), Expect = 0.75
 Identities = 11/33 (33%), Positives = 19/33 (57%)
 Frame = -3

Query: 380 ICYARVRTSASSLVEDASRLMCHTSSQSLPILY 282
           +CY+   T++  L+E    +  H  + SLPI+Y
Sbjct: 828 VCYSSFLTASPRLMEPVYMVEVHAPADSLPIIY 860


>SPBC646.03 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 471

 Score = 27.9 bits (59), Expect = 1.3
 Identities = 16/45 (35%), Positives = 22/45 (48%), Gaps = 1/45 (2%)
 Frame = +2

Query: 86  SVNENFPEYAVPGAKQHRFEPYADNGGSIVAIAGD-DYAVIGADT 217
           S N  F     P  K   ++    +GG+  AIA D  YA +G+DT
Sbjct: 118 SENNLFGRTVNPVVKDSNYDVGGSSGGAAAAIAADICYASVGSDT 162


>SPAC6C3.06c |||P-type ATPase, calcium
           transporting|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1033

 Score = 27.5 bits (58), Expect = 1.7
 Identities = 11/26 (42%), Positives = 15/26 (57%)
 Frame = +3

Query: 351 CRCTNTSITNL*PLRQSHRCSQLCCI 428
           CRCT T   N+  L Q  + + +CCI
Sbjct: 743 CRCTPTQKANMTRLIQEKKQASVCCI 768


>SPAC56E4.06c |ggt2||gamma-glutamyltranspeptidase
           Ggt2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 611

 Score = 26.2 bits (55), Expect = 4.0
 Identities = 12/28 (42%), Positives = 16/28 (57%)
 Frame = -1

Query: 253 VSGIYGKSSAETSVCSDNGIIIAGDGYN 170
           V G  G  ++E  VCSD G+ +  DG N
Sbjct: 77  VRGRRGAVASEVPVCSDIGVSMLADGGN 104


>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 646

 Score = 26.2 bits (55), Expect = 4.0
 Identities = 11/22 (50%), Positives = 14/22 (63%)
 Frame = +2

Query: 176 AIAGDDYAVIGADTRLSTGFSI 241
           AI GD Y+ IG++     GFSI
Sbjct: 321 AIVGDTYSTIGSNVEALPGFSI 342


>SPMIT.06 |||mitochondrial DNA binding
           endonuclease|Schizosaccharomyces pombe|chr
           mitochondrial|||Manual
          Length = 807

 Score = 25.4 bits (53), Expect = 7.0
 Identities = 10/28 (35%), Positives = 16/28 (57%)
 Frame = +2

Query: 506 YDPIGHCARHNFRAGGSAAAQLQPLLDN 589
           Y+P+ + A H FR G S  + L+ +  N
Sbjct: 332 YEPLFNTASHGFRPGRSCHSALRSIFTN 359


>SPBC365.02c |cox10||protoheme IX
           farnesyltransferase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 387

 Score = 25.4 bits (53), Expect = 7.0
 Identities = 14/58 (24%), Positives = 29/58 (50%)
 Frame = +3

Query: 57  FFHKNHTKC*ALTRTFLNMLCLVRNSIVSSPTRTTEVVL*PSPAMIMPLSEQTLVSAL 230
           F+H+++     L+RTF +   +  N + +  T T+     P P ++  LS++   + L
Sbjct: 23  FYHQHYEHTGKLSRTFFSPTHIKYNRLSTLDTSTSTANAAPDPQVLTFLSKRMQAAPL 80


>SPAC29B12.01 |ino80|SPAC3G6.12|SNF2 family helicase
           Ino80|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1604

 Score = 25.4 bits (53), Expect = 7.0
 Identities = 12/30 (40%), Positives = 17/30 (56%)
 Frame = +2

Query: 347 RMQMYEHEHNKSMTTPAVAQMLSTMLYYKR 436
           R Q    ++NK M T A   M  TM+++KR
Sbjct: 666 RWQFRTIKNNKDMQTKAKRAMRETMVFWKR 695


>SPBC1289.01c |chr4|cfh3, SPBC1539.11c|chitin synthase regulatory
           factor |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 633

 Score = 25.0 bits (52), Expect = 9.2
 Identities = 12/32 (37%), Positives = 18/32 (56%)
 Frame = +2

Query: 542 RAGGSAAAQLQPLLDNQIGLKNMQNVTEAPLP 637
           +AG S A +   LL+NQI     +N+T +  P
Sbjct: 540 KAGHSEAIERLALLENQIQEPEPENITSSQYP 571


>SPCC1183.08c |rpl101|rpl1-1, rpl10a-1|60S ribosomal protein
           L10a|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 216

 Score = 25.0 bits (52), Expect = 9.2
 Identities = 11/30 (36%), Positives = 18/30 (60%)
 Frame = -2

Query: 123 PGTAYSGKFSLTLNILYDFYGKISFIRFEI 34
           PG + +GKF   ++   D YGKI+ ++  I
Sbjct: 125 PGLSKAGKFPSPVSHADDLYGKITEVKSTI 154


>SPBC577.09 |||ERCC-8 homolog |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 404

 Score = 25.0 bits (52), Expect = 9.2
 Identities = 9/17 (52%), Positives = 12/17 (70%)
 Frame = +1

Query: 13  CMSLDSKYFEANKTNFS 63
           CM L +KY  +N+TN S
Sbjct: 228 CMDLHNKYLPSNQTNIS 244


>SPCC23B6.04c |||sec14 cytosolic factor family|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1008

 Score = 25.0 bits (52), Expect = 9.2
 Identities = 9/16 (56%), Positives = 11/16 (68%)
 Frame = +2

Query: 461 VLAGLDSDGKGCVYSY 508
           VL G D DG+ C+Y Y
Sbjct: 686 VLLGYDKDGRPCLYLY 701


>SPAC17G6.05c |||Rhophilin-2 homolog|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 775

 Score = 25.0 bits (52), Expect = 9.2
 Identities = 14/62 (22%), Positives = 27/62 (43%)
 Frame = +2

Query: 104 PEYAVPGAKQHRFEPYADNGGSIVAIAGDDYAVIGADTRLSTGFSIYTRDQKKLFKLSES 283
           P  A+P     +     D   +   I GDD       + ++T  S+Y+ +  K+F+  ++
Sbjct: 346 PLQALPPLPLEKLYGSQDGFETAKKIVGDDLFKAFVPSAVTTASSLYSEETAKVFRAEQA 405

Query: 284 TV 289
            V
Sbjct: 406 EV 407


>SPBC26H8.12 |||cytochrome c heme lyase|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 377

 Score = 25.0 bits (52), Expect = 9.2
 Identities = 21/73 (28%), Positives = 29/73 (39%)
 Frame = +2

Query: 356 MYEHEHNKSMTTPAVAQMLSTMLYYKRFFPYYVSNVLAGLDSDGKGCVYSYDPIGHCARH 535
           ++  E   S  TPAV    +  +      P  VS VL G+DS  +  V    P+    R 
Sbjct: 105 LFGREKQNSEATPAVQPPATCPMSNSNQKPAGVSEVLTGVDSKQQSYVPEGCPVATPKRG 164

Query: 536 NFRAGGSAAAQLQ 574
            F   G+   Q Q
Sbjct: 165 WFNWFGNNDDQKQ 177


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,771,090
Number of Sequences: 5004
Number of extensions: 58148
Number of successful extensions: 196
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 189
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 196
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 285732116
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -