BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fner10b06f (640 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC22F8.06 |pam1||20S proteasome component beta 6|Schizosacchar... 162 3e-41 SPCC63.12c |||20S proteasome component beta 3|Schizosaccharomyce... 83 2e-17 SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyc... 51 1e-07 SPAC23D3.07 |pup1||20S proteasome component beta 2|Schizosacchar... 45 8e-06 SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Sch... 30 0.25 SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 29 0.75 SPBC646.03 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces... 28 1.3 SPAC6C3.06c |||P-type ATPase, calcium transporting|Schizosacchar... 27 1.7 SPAC56E4.06c |ggt2||gamma-glutamyltranspeptidase Ggt2|Schizosacc... 26 4.0 SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 26 4.0 SPMIT.06 |||mitochondrial DNA binding endonuclease|Schizosacchar... 25 7.0 SPBC365.02c |cox10||protoheme IX farnesyltransferase|Schizosacch... 25 7.0 SPAC29B12.01 |ino80|SPAC3G6.12|SNF2 family helicase Ino80|Schizo... 25 7.0 SPBC1289.01c |chr4|cfh3, SPBC1539.11c|chitin synthase regulatory... 25 9.2 SPCC1183.08c |rpl101|rpl1-1, rpl10a-1|60S ribosomal protein L10a... 25 9.2 SPBC577.09 |||ERCC-8 homolog |Schizosaccharomyces pombe|chr 2|||... 25 9.2 SPCC23B6.04c |||sec14 cytosolic factor family|Schizosaccharomyce... 25 9.2 SPAC17G6.05c |||Rhophilin-2 homolog|Schizosaccharomyces pombe|ch... 25 9.2 SPBC26H8.12 |||cytochrome c heme lyase|Schizosaccharomyces pombe... 25 9.2 >SPAC22F8.06 |pam1||20S proteasome component beta 6|Schizosaccharomyces pombe|chr 1|||Manual Length = 225 Score = 162 bits (394), Expect = 3e-41 Identities = 73/159 (45%), Positives = 105/159 (66%) Frame = +2 Query: 131 QHRFEPYADNGGSIVAIAGDDYAVIGADTRLSTGFSIYTRDQKKLFKLSESTVLGATGCW 310 Q +F+PY NGG+ VAIAGD +A++ DTR G++I TR Q ++ ++ + V+GA+G Sbjct: 3 QSQFDPYVQNGGTTVAIAGDGFAILAGDTRSVNGYNINTRFQPRVHEVGDDLVIGASGFE 62 Query: 311 CDTLTLTRLLQARMQMYEHEHNKSMTTPAVAQMLSTMLYYKRFFPYYVSNVLAGLDSDGK 490 D L L + +Q R+ +Y H + M+ + A M+ T+LY KRFFPYYV +AG+D +GK Sbjct: 63 ADALALVKRIQQRIDLYHDNHERKMSAQSCACMVRTLLYGKRFFPYYVYTTVAGIDKEGK 122 Query: 491 GCVYSYDPIGHCARHNFRAGGSAAAQLQPLLDNQIGLKN 607 G +YS+DP+G R RAGGSAA + P LDNQ+ L N Sbjct: 123 GEIYSFDPVGSYEREWCRAGGSAANFITPFLDNQVNLHN 161 >SPCC63.12c |||20S proteasome component beta 3|Schizosaccharomyces pombe|chr 3|||Manual Length = 204 Score = 83.4 bits (197), Expect = 2e-17 Identities = 47/146 (32%), Positives = 75/146 (51%), Gaps = 2/146 (1%) Frame = +2 Query: 158 NGGSIVAIAGDDYAVIGADTRLSTGFSIYTRDQKKLFKLSESTVLGATGCWCDTLTLTRL 337 NGGS VA+AG + I +D RL T + K+F + + T LG TG D TL L Sbjct: 7 NGGSCVAMAGKNCVAIASDLRLGVQSISLTNNFPKVFAMGDKTYLGLTGLATDVQTLYEL 66 Query: 338 LQARMQMYEHEHNKSMTTPAVAQMLSTMLYYKRFFPYYVSNVLAGLDSDGKGCVYSYDPI 517 + ++ +Y+ + + A ++S+ LY KRF PY+ V+AG+ +D + +D I Sbjct: 67 FRYKVNLYKFREERQIQPKTFANLVSSTLYEKRFGPYFSFPVVAGVSNDNTPFICGFDSI 126 Query: 518 GHCA--RHNFRAGGSAAAQLQPLLDN 589 G C +F G+A QL + ++ Sbjct: 127 G-CIDFAEDFIVSGTATEQLYGMCES 151 >SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 51.2 bits (117), Expect = 1e-07 Identities = 38/142 (26%), Positives = 61/142 (42%) Frame = +2 Query: 161 GGSIVAIAGDDYAVIGADTRLSTGFSIYTRDQKKLFKLSESTVLGATGCWCDTLTLTRLL 340 G +I A+ D ++ AD+R + G I R KL +L+++ +G DT T+ LL Sbjct: 24 GTTITALRYKDGVILAADSRTTMGAYIANRVTDKLTQLTDNIWCCRSGSAADTQTVADLL 83 Query: 341 QARMQMYEHEHNKSMTTPAVAQMLSTMLYYKRFFPYYVSNVLAGLDSDGKGCVYSYDPIG 520 + + MY + + A + S M Y + ++AG D G VYS G Sbjct: 84 KYYLSMYRIQFGHDPSVHTAATLASEMCYQNKNM-LSAGLIVAGYDEKTGGDVYSIPLGG 142 Query: 521 HCARHNFRAGGSAAAQLQPLLD 586 + GGS +A + D Sbjct: 143 SLHKQPLAIGGSGSAFIYGFCD 164 >SPAC23D3.07 |pup1||20S proteasome component beta 2|Schizosaccharomyces pombe|chr 1|||Manual Length = 267 Score = 45.2 bits (102), Expect = 8e-06 Identities = 29/110 (26%), Positives = 52/110 (47%), Gaps = 1/110 (0%) Frame = +2 Query: 161 GGSIVAIAGDDYAVIGADTRLSTGFSIYTRDQKKLFKLSESTVLGATGCWCDTLTLTRLL 340 G +IV + D V+GADTR + G I ++ KKL +S + G DT +T ++ Sbjct: 35 GTTIVGVIAKDCIVLGADTRATAGPIIADKNCKKLHLISPNIWCAGAGTAADTEFVTSMI 94 Query: 341 QARMQMYE-HEHNKSMTTPAVAQMLSTMLYYKRFFPYYVSNVLAGLDSDG 487 + ++++ + + K A+ + + Y+ Y+ VL G D G Sbjct: 95 SSNIELHSLYTNRKPRVVTALTMLKQHLFRYQGHIGAYL--VLGGYDCKG 142 >SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Schizosaccharomyces pombe|chr 1|||Manual Length = 248 Score = 30.3 bits (65), Expect = 0.25 Identities = 30/162 (18%), Positives = 64/162 (39%), Gaps = 4/162 (2%) Frame = +2 Query: 155 DNGGSIVAIAGDDYAVIGADTRLSTGFSIYTRDQKKLFKLSESTVLGATGCWCDTLTLTR 334 ++ G + I D V+ A+ ++++ +KL+ + ++ + G D L Sbjct: 29 NHAGVALGIVAKDGIVLAAEKKVTSKLLEQEESAEKLYHIGDNMLCAVAGLTADANILIN 88 Query: 335 LLQARMQMYEHEHNKSMTTPAVAQMLSTM----LYYKRFFPYYVSNVLAGLDSDGKGCVY 502 + Q Y N+ M + + + + Y P+ VS + AG D ++ Sbjct: 89 YARRVGQQYLQTFNEEMPCEQLVRRVCDLKQGYTQYGGLRPFGVSFLYAGWDHIRGYQLF 148 Query: 503 SYDPIGHCARHNFRAGGSAAAQLQPLLDNQIGLKNMQNVTEA 628 +P G+ + G + +Q L+ + K+ N+ EA Sbjct: 149 QSNPSGNYGSWQANSIGGNSTSVQSLMRQE--YKDDINLDEA 188 >SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces pombe|chr 2|||Manual Length = 983 Score = 28.7 bits (61), Expect = 0.75 Identities = 11/33 (33%), Positives = 19/33 (57%) Frame = -3 Query: 380 ICYARVRTSASSLVEDASRLMCHTSSQSLPILY 282 +CY+ T++ L+E + H + SLPI+Y Sbjct: 828 VCYSSFLTASPRLMEPVYMVEVHAPADSLPIIY 860 >SPBC646.03 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces pombe|chr 2|||Manual Length = 471 Score = 27.9 bits (59), Expect = 1.3 Identities = 16/45 (35%), Positives = 22/45 (48%), Gaps = 1/45 (2%) Frame = +2 Query: 86 SVNENFPEYAVPGAKQHRFEPYADNGGSIVAIAGD-DYAVIGADT 217 S N F P K ++ +GG+ AIA D YA +G+DT Sbjct: 118 SENNLFGRTVNPVVKDSNYDVGGSSGGAAAAIAADICYASVGSDT 162 >SPAC6C3.06c |||P-type ATPase, calcium transporting|Schizosaccharomyces pombe|chr 1|||Manual Length = 1033 Score = 27.5 bits (58), Expect = 1.7 Identities = 11/26 (42%), Positives = 15/26 (57%) Frame = +3 Query: 351 CRCTNTSITNL*PLRQSHRCSQLCCI 428 CRCT T N+ L Q + + +CCI Sbjct: 743 CRCTPTQKANMTRLIQEKKQASVCCI 768 >SPAC56E4.06c |ggt2||gamma-glutamyltranspeptidase Ggt2|Schizosaccharomyces pombe|chr 1|||Manual Length = 611 Score = 26.2 bits (55), Expect = 4.0 Identities = 12/28 (42%), Positives = 16/28 (57%) Frame = -1 Query: 253 VSGIYGKSSAETSVCSDNGIIIAGDGYN 170 V G G ++E VCSD G+ + DG N Sbjct: 77 VRGRRGAVASEVPVCSDIGVSMLADGGN 104 >SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 646 Score = 26.2 bits (55), Expect = 4.0 Identities = 11/22 (50%), Positives = 14/22 (63%) Frame = +2 Query: 176 AIAGDDYAVIGADTRLSTGFSI 241 AI GD Y+ IG++ GFSI Sbjct: 321 AIVGDTYSTIGSNVEALPGFSI 342 >SPMIT.06 |||mitochondrial DNA binding endonuclease|Schizosaccharomyces pombe|chr mitochondrial|||Manual Length = 807 Score = 25.4 bits (53), Expect = 7.0 Identities = 10/28 (35%), Positives = 16/28 (57%) Frame = +2 Query: 506 YDPIGHCARHNFRAGGSAAAQLQPLLDN 589 Y+P+ + A H FR G S + L+ + N Sbjct: 332 YEPLFNTASHGFRPGRSCHSALRSIFTN 359 >SPBC365.02c |cox10||protoheme IX farnesyltransferase|Schizosaccharomyces pombe|chr 2|||Manual Length = 387 Score = 25.4 bits (53), Expect = 7.0 Identities = 14/58 (24%), Positives = 29/58 (50%) Frame = +3 Query: 57 FFHKNHTKC*ALTRTFLNMLCLVRNSIVSSPTRTTEVVL*PSPAMIMPLSEQTLVSAL 230 F+H+++ L+RTF + + N + + T T+ P P ++ LS++ + L Sbjct: 23 FYHQHYEHTGKLSRTFFSPTHIKYNRLSTLDTSTSTANAAPDPQVLTFLSKRMQAAPL 80 >SPAC29B12.01 |ino80|SPAC3G6.12|SNF2 family helicase Ino80|Schizosaccharomyces pombe|chr 1|||Manual Length = 1604 Score = 25.4 bits (53), Expect = 7.0 Identities = 12/30 (40%), Positives = 17/30 (56%) Frame = +2 Query: 347 RMQMYEHEHNKSMTTPAVAQMLSTMLYYKR 436 R Q ++NK M T A M TM+++KR Sbjct: 666 RWQFRTIKNNKDMQTKAKRAMRETMVFWKR 695 >SPBC1289.01c |chr4|cfh3, SPBC1539.11c|chitin synthase regulatory factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 633 Score = 25.0 bits (52), Expect = 9.2 Identities = 12/32 (37%), Positives = 18/32 (56%) Frame = +2 Query: 542 RAGGSAAAQLQPLLDNQIGLKNMQNVTEAPLP 637 +AG S A + LL+NQI +N+T + P Sbjct: 540 KAGHSEAIERLALLENQIQEPEPENITSSQYP 571 >SPCC1183.08c |rpl101|rpl1-1, rpl10a-1|60S ribosomal protein L10a|Schizosaccharomyces pombe|chr 3|||Manual Length = 216 Score = 25.0 bits (52), Expect = 9.2 Identities = 11/30 (36%), Positives = 18/30 (60%) Frame = -2 Query: 123 PGTAYSGKFSLTLNILYDFYGKISFIRFEI 34 PG + +GKF ++ D YGKI+ ++ I Sbjct: 125 PGLSKAGKFPSPVSHADDLYGKITEVKSTI 154 >SPBC577.09 |||ERCC-8 homolog |Schizosaccharomyces pombe|chr 2|||Manual Length = 404 Score = 25.0 bits (52), Expect = 9.2 Identities = 9/17 (52%), Positives = 12/17 (70%) Frame = +1 Query: 13 CMSLDSKYFEANKTNFS 63 CM L +KY +N+TN S Sbjct: 228 CMDLHNKYLPSNQTNIS 244 >SPCC23B6.04c |||sec14 cytosolic factor family|Schizosaccharomyces pombe|chr 3|||Manual Length = 1008 Score = 25.0 bits (52), Expect = 9.2 Identities = 9/16 (56%), Positives = 11/16 (68%) Frame = +2 Query: 461 VLAGLDSDGKGCVYSY 508 VL G D DG+ C+Y Y Sbjct: 686 VLLGYDKDGRPCLYLY 701 >SPAC17G6.05c |||Rhophilin-2 homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 775 Score = 25.0 bits (52), Expect = 9.2 Identities = 14/62 (22%), Positives = 27/62 (43%) Frame = +2 Query: 104 PEYAVPGAKQHRFEPYADNGGSIVAIAGDDYAVIGADTRLSTGFSIYTRDQKKLFKLSES 283 P A+P + D + I GDD + ++T S+Y+ + K+F+ ++ Sbjct: 346 PLQALPPLPLEKLYGSQDGFETAKKIVGDDLFKAFVPSAVTTASSLYSEETAKVFRAEQA 405 Query: 284 TV 289 V Sbjct: 406 EV 407 >SPBC26H8.12 |||cytochrome c heme lyase|Schizosaccharomyces pombe|chr 2|||Manual Length = 377 Score = 25.0 bits (52), Expect = 9.2 Identities = 21/73 (28%), Positives = 29/73 (39%) Frame = +2 Query: 356 MYEHEHNKSMTTPAVAQMLSTMLYYKRFFPYYVSNVLAGLDSDGKGCVYSYDPIGHCARH 535 ++ E S TPAV + + P VS VL G+DS + V P+ R Sbjct: 105 LFGREKQNSEATPAVQPPATCPMSNSNQKPAGVSEVLTGVDSKQQSYVPEGCPVATPKRG 164 Query: 536 NFRAGGSAAAQLQ 574 F G+ Q Q Sbjct: 165 WFNWFGNNDDQKQ 177 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,771,090 Number of Sequences: 5004 Number of extensions: 58148 Number of successful extensions: 196 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 189 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 196 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 285732116 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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