BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner10a13f
(631 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g39010.1 68417.m05526 glycosyl hydrolase family 9 protein end... 34 0.089
At5g27730.1 68418.m03326 expressed protein 29 1.9
At3g13790.1 68416.m01742 beta-fructosidase (BFRUCT1) / beta-fruc... 29 2.5
At5g10370.1 68418.m01203 helicase domain-containing protein / IB... 29 3.4
At2g31250.1 68415.m03816 glutamyl-tRNA reductase, putative simil... 29 3.4
At4g33720.1 68417.m04788 pathogenesis-related protein, putative ... 28 5.9
At4g12750.1 68417.m02002 expressed protein 28 5.9
At4g26800.1 68417.m03860 pentatricopeptide (PPR) repeat-containi... 27 7.8
At4g26560.1 68417.m03828 calcineurin B-like protein, putative si... 27 7.8
At4g18600.1 68417.m02755 expressed protein 27 7.8
>At4g39010.1 68417.m05526 glycosyl hydrolase family 9 protein
endo-1,4-beta-glucanase precursor - Fragariax ananassa,
PID:g3549291
Length = 497
Score = 33.9 bits (74), Expect = 0.089
Identities = 22/85 (25%), Positives = 37/85 (43%)
Frame = +3
Query: 372 YTDMLLEPAMWAHNPTMLDDLVNISHFSWSPVGFIQNNESALAILNNVGQVDIIAPKENR 551
+ D LL A W T + +N + P G +N N VG ++++ KE
Sbjct: 240 FQDELLWGAAWLRKATGDESYLNYIESNREPFG-ANDNVDEFGWDNKVGGLNVLVSKEVI 298
Query: 552 WVNVFNLSPYLSHSLKIQASMSPQS 626
N++NL Y + + S+ P+S
Sbjct: 299 EGNMYNLEAYKASAESFMCSLVPES 323
>At5g27730.1 68418.m03326 expressed protein
Length = 472
Score = 29.5 bits (63), Expect = 1.9
Identities = 13/47 (27%), Positives = 26/47 (55%)
Frame = -2
Query: 258 ILN*VRIHTFVFLYHDERLARFFATISSIPLFY*LLLSVYEFQKVYF 118
++N +R H F+ ++H R+ I + LF+ L+ V+ K+Y+
Sbjct: 424 LINWIREHVFIRVWHSRRVGVLMYVIFAEILFWGLVTGVFHRFKIYW 470
>At3g13790.1 68416.m01742 beta-fructosidase (BFRUCT1) /
beta-fructofuranosidase / cell wall invertase identical
to beta-fructofuranosidase GI:402740 from [Arabidopsis
thaliana]
Length = 584
Score = 29.1 bits (62), Expect = 2.5
Identities = 11/36 (30%), Positives = 20/36 (55%)
Frame = +3
Query: 132 EIHRRSITINKIEESTISWQKNVLAVRHDKGIQMYE 239
+IHRR + I + + W+K+ + +D G M+E
Sbjct: 211 KIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGMWE 246
>At5g10370.1 68418.m01203 helicase domain-containing protein / IBR
domain-containing protein / zinc finger protein-related
similar to RNA-dependent ATPase/helicase Cdc28p
[Schizosaccharomyces pombe] GI:1439562; contains Pfam
profiles PF04408: Helicase associated domain (HA2),
PF00271: Helicase conserved C-terminal domain, weak hit
to PF00097: Zinc finger, C3HC4 type (RING finger),
PF01485: IBR domain
Length = 1775
Score = 28.7 bits (61), Expect = 3.4
Identities = 11/18 (61%), Positives = 15/18 (83%)
Frame = +1
Query: 523 LILLLPKKIDGSMCSIFR 576
L+ L KKIDGS+CSI++
Sbjct: 1085 LLTFLEKKIDGSICSIYK 1102
>At2g31250.1 68415.m03816 glutamyl-tRNA reductase, putative similar
to HEMA2 [SP|P49294], HEMA1 [SP|P42804]
Length = 524
Score = 28.7 bits (61), Expect = 3.4
Identities = 23/109 (21%), Positives = 50/109 (45%), Gaps = 4/109 (3%)
Frame = +3
Query: 297 PTTKTSPAEKLFSGQVFDRYTPNMLYTDMLLEPAMWAHNPTMLDDLVNISHFSWSPVGFI 476
P++ +SP +KL + DRYT + +++ + +P + + + I W P+
Sbjct: 57 PSSDSSPLDKLKNSPAIDRYTRER--SSIVVIGLSFHTSPLEMRERLAIPEAEW-PLAIT 113
Query: 477 Q----NNESALAILNNVGQVDIIAPKENRWVNVFNLSPYLSHSLKIQAS 611
Q N+ A+L+ +++I +R+ V ++ ++S I S
Sbjct: 114 QLCALNHIEEAAVLSTCNRIEIYVSALSRYRGVKEVTEWMSKRSGIPVS 162
>At4g33720.1 68417.m04788 pathogenesis-related protein, putative
similar to SP|P33154 Pathogenesis-related protein 1
precursor (PR-1) {Arabidopsis thaliana}; contains Pfam
profile PF00188: SCP-like extracellular protein
Length = 163
Score = 27.9 bits (59), Expect = 5.9
Identities = 8/13 (61%), Positives = 11/13 (84%)
Frame = +1
Query: 379 TCFWNRRCGHITQ 417
TC W+++CGH TQ
Sbjct: 112 TCAWDKQCGHYTQ 124
>At4g12750.1 68417.m02002 expressed protein
Length = 1108
Score = 27.9 bits (59), Expect = 5.9
Identities = 13/29 (44%), Positives = 21/29 (72%)
Frame = -1
Query: 430 SSSIVGLCAHIAGSKSMSVYNIFGVYLSN 344
SSSIVG A +AG+++ +V+ + VYL +
Sbjct: 723 SSSIVGAFAKLAGNRANNVHPMQSVYLGS 751
>At4g26800.1 68417.m03860 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 369
Score = 27.5 bits (58), Expect = 7.8
Identities = 16/51 (31%), Positives = 27/51 (52%)
Frame = -1
Query: 553 HLFSLGAIISTWPTLFKIAKADSLFCMKPTGDQEKWLMFTKSSSIVGLCAH 401
++ + A+I + K++K DS++ M + +FT SS I GLC H
Sbjct: 117 NVITFSALIDAYAKRGKLSKVDSVYKMMIQMSIDP-NVFTYSSLIYGLCMH 166
>At4g26560.1 68417.m03828 calcineurin B-like protein, putative
similar to calcineurin B-like protein 3 [Arabidopsis
thaliana] GI:3309086, calcineurin B-like protein 2
[Arabidopsis thaliana] GI:3309084; contains
INTERPRO:IPR002048 calcium-binding EF-hand domain
Length = 214
Score = 27.5 bits (58), Expect = 7.8
Identities = 20/79 (25%), Positives = 34/79 (43%), Gaps = 5/79 (6%)
Frame = +3
Query: 258 CIDKNIDCSEIHVPTTKTSPAEKLFSGQVFDRYTPN---MLYTDMLLEPAMWAHNPTMLD 428
C ++ + HV +T E LFS +VFD + N +L + H +D
Sbjct: 50 CYYGEMNKEQFHVAIFQTDKNESLFSERVFDLFDTNHDGLLGFEEFARALSVFHPSAPID 109
Query: 429 DLVNISH--FSWSPVGFIQ 479
D +++S + GFI+
Sbjct: 110 DKIDLSFQLYDLKQQGFIE 128
>At4g18600.1 68417.m02755 expressed protein
Length = 1907
Score = 27.5 bits (58), Expect = 7.8
Identities = 15/37 (40%), Positives = 18/37 (48%)
Frame = +3
Query: 237 EFSLNLECIDKNIDCSEIHVPTTKTSPAEKLFSGQVF 347
E SLN D+ I SE TT+T P K F + F
Sbjct: 1258 ENSLNEAVFDEKIPGSEASTSTTETGPHNKTFPEEPF 1294
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,181,694
Number of Sequences: 28952
Number of extensions: 270199
Number of successful extensions: 639
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 627
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 639
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1285411824
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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