BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV1e12r
(780 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi... 30 0.43
SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit ... 28 1.7
SPBC1E8.03c |||conserved fungal protein|Schizosaccharomyces pomb... 27 2.3
SPBC25H2.02 |ths1||threonine-tRNA ligase Ths1 |Schizosaccharomyc... 25 9.2
>SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase
Lkh1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 690
Score = 29.9 bits (64), Expect = 0.43
Identities = 15/47 (31%), Positives = 21/47 (44%), Gaps = 2/47 (4%)
Frame = -3
Query: 442 PAQHASSFHTPLSYTISSLT--PPLTNIVFH*VWWAPKNTPNPNLAH 308
P H H PL + + PP+ + H W P + P+PNL H
Sbjct: 134 PHSHHPPLHNPLPVSCQPVLRPPPVPQVPSH---WYPVSLPSPNLPH 177
>SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit
Sec63 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 611
Score = 27.9 bits (59), Expect = 1.7
Identities = 14/33 (42%), Positives = 18/33 (54%), Gaps = 1/33 (3%)
Frame = +1
Query: 433 VVP-APGDWEKGWRMMMMKYVHVPPGVKWFPKL 528
V+P A G W G R +VHV +WFPK+
Sbjct: 206 VLPYAVGKWWYGSRTYTRDHVHVDTVDEWFPKM 238
>SPBC1E8.03c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 477
Score = 27.5 bits (58), Expect = 2.3
Identities = 13/51 (25%), Positives = 25/51 (49%)
Frame = -2
Query: 509 TPGGT*TYFIIIILQPFSQSPGAGTTCFFLPYSSIIYHFFAHSPSYKYRLS 357
TP TY +++ LQP S + +L Y ++ ++ S +YK ++
Sbjct: 327 TPPNDYTYALVVDLQPDSPPQLSSNLITWLKYKILLIYYHKSSSTYKNNIA 377
>SPBC25H2.02 |ths1||threonine-tRNA ligase Ths1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 703
Score = 25.4 bits (53), Expect = 9.2
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = -2
Query: 452 SPGAGTTCFFLPYSSIIYHFFAHSPSYKY 366
SPG+ CFFLP+ + IY+ Y+Y
Sbjct: 307 SPGS---CFFLPHGARIYNTLLKYMRYQY 332
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,183,659
Number of Sequences: 5004
Number of extensions: 64428
Number of successful extensions: 141
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 139
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 141
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 377352472
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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